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Team:Alberta/Tour/biobytes
From 2010.igem.org
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The method has three main components: | The method has three main components: | ||
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==The Anchor== | ==The Anchor== | ||
A ferro-magnetic bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct. The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier. | A ferro-magnetic bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct. The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier. | ||
| - | + | [[Image:team-alberta-anchor-tour.jpg|center]] | |
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| - | [[Image:team-alberta- | + | |
==The bytes== | ==The bytes== | ||
DNA fragments that can be attached together to build up a larger construct. There are two types of pieces, AB and BA. The A end can join only with another A end and the B end can join only with another B end. As a result pieces can only be joined in a single orientation. | DNA fragments that can be attached together to build up a larger construct. There are two types of pieces, AB and BA. The A end can join only with another A end and the B end can join only with another B end. As a result pieces can only be joined in a single orientation. | ||
| - | + | [[Image:team-alberta-bytes-tour.jpg|center]] | |
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| - | [[Image:team-alberta- | + | |
==The cap== | ==The cap== | ||
A DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid. | A DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid. | ||
| - | + | [[Image:team-alberta-cap-tour.jpg|center]] | |
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| - | [[Image:team-alberta- | + | ==Putting the pieces together== |
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The process of building a plasmid is more elegant and more rapid than the current biobyte system! | The process of building a plasmid is more elegant and more rapid than the current biobyte system! | ||
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[[Image:team-alberta-biobyteprocess-tour.jpg]] | [[Image:team-alberta-biobyteprocess-tour.jpg]] | ||
| - | + | Easy! And fast too, each step takes about 7 minutes. | |
| - | + | [[Image:team-alberta-building-tour.jpg|center]] | |
| - | + | Compare that to the standard biobrick method: | |
| - | + | [[Image:team-alberta-biobrick-tour.jpg|center]] | |
| - | + | Using this process we were able to assemble eight pieces in an afternoon! | |
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{{Team:Alberta/endMainContent|class=not-top}} | {{Team:Alberta/endMainContent|class=not-top}} | ||
Revision as of 15:42, 26 October 2010
The Anchor
A ferro-magnetic bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct. The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier.
The bytes
DNA fragments that can be attached together to build up a larger construct. There are two types of pieces, AB and BA. The A end can join only with another A end and the B end can join only with another B end. As a result pieces can only be joined in a single orientation.
The cap
A DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.
Putting the pieces together
The process of building a plasmid is more elegant and more rapid than the current biobyte system!
Easy! And fast too, each step takes about 7 minutes.
Compare that to the standard biobrick method:
Using this process we were able to assemble eight pieces in an afternoon!
