Team:Alberta/Tour/biobytes

From 2010.igem.org

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[[Image:team-alberta-cap-tour.jpg]]  
[[Image:team-alberta-cap-tour.jpg]]  
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The cap: a DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.
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The cap: a DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.
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Starting with an anchor, add the first piece and ligate.
Starting with an anchor, add the first piece and ligate.
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Then hold the single piece construct in the tube by placing it on the magnetic rack now you can wash away most of the excess of piece 1.
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Then hold the single piece construct in the tube by placing it on the magnetic rack.  Now you can wash away most of the excess of piece 1.
Add the next piece and repeat until you have added all the pieces you want.
Add the next piece and repeat until you have added all the pieces you want.
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Then add the cap.
Then add the cap.
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Now just heat to release the the anchor and open up the cap and upon cooling the construct will circularize.   
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Now just heat to release the anchor and open up the cap, upon cooling the construct will circularize.   
Easy!
Easy!
Using this process we were able to assemble eight pieces in an afternoon!
Using this process we were able to assemble eight pieces in an afternoon!

Revision as of 03:47, 26 October 2010

TEAM ALBERTA

We continued to develop the biobytes assembly method developed by last year’s team. The method has three main components:

Team-alberta-anchor-tour.jpg

The Anchor: a ferro-magnetic bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct. The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier.

Team-alberta-bytes-tour.jpg

The bytes: DNA fragments that can be attached together to build up a larger construct. There are two types of pieces, AB and BA. The A end can join only with another A end and the B end can join only with another B end. As a result pieces can only be joined in a single orientation.


Team-alberta-cap-tour.jpg

The cap: a DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.


Team-alberta-biobyteprocess-tour.jpg

The process of building a plasmid is elegant and rapid!

Team-alberta-building-tour.jpg

Team-alberta-biobrick-tour.jpg


Starting with an anchor, add the first piece and ligate.

Then hold the single piece construct in the tube by placing it on the magnetic rack. Now you can wash away most of the excess of piece 1.

Add the next piece and repeat until you have added all the pieces you want.

Then add the cap.

Now just heat to release the anchor and open up the cap, upon cooling the construct will circularize.

Easy!

Using this process we were able to assemble eight pieces in an afternoon!