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Team:Alberta/Tour/biobytes
From 2010.igem.org
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{{Team:Alberta/beginMainContent|class=not-top}} | {{Team:Alberta/beginMainContent|class=not-top}} | ||
| - | We continued to develop the | + | We continued to develop the BioBytes assembly method developed by last year’s team. |
The method has three main components: | The method has three main components: | ||
==The Anchor== | ==The Anchor== | ||
| - | + | An iron micro bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct. The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier. | |
[[Image:team-alberta-anchor-tour.jpg|center]] | [[Image:team-alberta-anchor-tour.jpg|center]] | ||
| - | ==The | + | ==The Bytes== |
| - | DNA fragments that can be attached together to build up a larger construct. There are two types | + | DNA fragments that can be attached together to build up a larger construct. There are two types of pieces, AB and BA. The A end can join only with another A end and the B end can join only with another B end. As a result, pieces can only be joined in a single orientation. |
[[Image:team-alberta-bytes-tour.jpg|center]] | [[Image:team-alberta-bytes-tour.jpg|center]] | ||
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==Putting the pieces together== | ==Putting the pieces together== | ||
| - | The process of building a plasmid is more elegant and more rapid than the current | + | The process of building a plasmid is more elegant and more rapid than the current BioByte system! |
[[Image:team-alberta-biobyteprocess-tour.jpg]] | [[Image:team-alberta-biobyteprocess-tour.jpg]] | ||
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[[Image:team-alberta-building-tour.jpg|center]] | [[Image:team-alberta-building-tour.jpg|center]] | ||
| - | Compare that to the standard | + | Compare that to the standard BioBrick method: |
[[Image:team-alberta-biobrick-tour.jpg|center]] | [[Image:team-alberta-biobrick-tour.jpg|center]] | ||
Revision as of 00:36, 27 October 2010
The Anchor
An iron micro bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct. The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier.
The Bytes
DNA fragments that can be attached together to build up a larger construct. There are two types of pieces, AB and BA. The A end can join only with another A end and the B end can join only with another B end. As a result, pieces can only be joined in a single orientation.
The cap
A DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.
Putting the pieces together
The process of building a plasmid is more elegant and more rapid than the current BioByte system!
Easy! And fast too, each step takes about 7 minutes.
Compare that to the standard BioBrick method:
Using this process we were able to assemble eight pieces in an afternoon!
