Team:Alberta/Notebook/protocols/miniprep

From 2010.igem.org

Revision as of 17:06, 26 October 2010 by Wiltshir (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

TEAM ALBERTA

Protocol 4: Plasmid Miniprep


Reagents:

  • Overnight culture
  • Qiagen plasmid miniprep kit


Procedure:

  • Transfer 1.5ml of culture to Eppendorf.
  • Spin down at 13 000 rpm.
  • Discard supernatant and follow procedure on p. 22 of QIAprep Miniprep Handbook.
  • Determine concentration of purified plasmid with nanodrop.


Notes:

  • Low speed pellet is easier to resuspend.
  • To resuspend, take an empty micro centrifuge rack and rub the tube quickly over the apparatus and vortex.
  • Step 2 in Qiagen, the alkaline lysis with buffer P2, should be brief to avoid damaging DNA.


Back

Retrieved from "http://2010.igem.org/Team:Alberta/Notebook/protocols/miniprep"