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Team:Alberta/Notebook/protocols/miniprep
From 2010.igem.org
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{{Team:Alberta/beginMainContent}} | {{Team:Alberta/beginMainContent}} | ||
| - | Plasmid Miniprep | + | ==Plasmid Miniprep== |
| - | Reagents: | + | ===Reagents:=== |
* Overnight culture | * Overnight culture | ||
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| - | Procedure: | + | ===Procedure:=== |
* Transfer 1.5ml of culture to Eppendorf. | * Transfer 1.5ml of culture to Eppendorf. | ||
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| - | Notes: | + | ===Notes:=== |
* Low speed pellet is easier to resuspend. | * Low speed pellet is easier to resuspend. | ||
* To resuspend, take an empty micro centrifuge rack and rub the tube quickly over the apparatus and vortex. | * To resuspend, take an empty micro centrifuge rack and rub the tube quickly over the apparatus and vortex. | ||
Revision as of 00:40, 27 October 2010
Plasmid Miniprep
Reagents:
- Overnight culture
- Qiagen plasmid miniprep kit
Procedure:
- Transfer 1.5ml of culture to Eppendorf.
- Spin down at 13 000 rpm.
- Discard supernatant and follow procedure on p. 22 of QIAprep Miniprep Handbook.
- Determine concentration of purified plasmid with nanodrop.
Notes:
- Low speed pellet is easier to resuspend.
- To resuspend, take an empty micro centrifuge rack and rub the tube quickly over the apparatus and vortex.
- Step 2 in Qiagen, the alkaline lysis with buffer P2, should be brief to avoid damaging DNA.
