Team:Alberta/Notebook/protocols/gel extraction

From 2010.igem.org

Revision as of 17:07, 26 October 2010 by Wiltshir (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

TEAM ALBERTA

Protocol 7: Gel Extraction


Reagents:

  • Clean scalpel
  • Transilluminator
  • Eppendorf tube
  • Gel extraction kit
  • 55oC water bath
  • Vortex
  • Microfuge


Procedure:

  • Place the gel on the transilluminator. Put on face shield and gloves.
  • Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
  • Pry out the gel ‘cube’ and place it in an eppendorf tube.
  • Follow instructions on pp. 25 of QIAQuick Spin Handbook.


Back