Team:Alberta/Notebook/protocols/gel extraction

From 2010.igem.org

(Difference between revisions)
 
Line 1: Line 1:
{{Team:Alberta/Head}}
{{Team:Alberta/Head}}
-
{{Team:Alberta/navbar|project=selected}}
+
{{Team:Alberta/navbar|notebook=selected}}
 +
 
 +
{{Team:Alberta/beginLeftSideBar|toc=NO}}
 +
 
 +
 
 +
==General Protocols==
 +
-----------------------------
 +
*[[Team:Alberta/Notebook/protocols/invitro_biobyte_assembly | In Vitro BioByte Assembly]]
 +
-----------------------------
 +
*[[Team:Alberta/Notebook/protocols/LB | LB Plates and Broth]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/transformations |Transformations]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/overnight |5mL Overnight ]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/glycerol | Glycerol Stock ]]
 +
-----------------------------
 +
*[[Team:Alberta/Notebook/protocols/miniprep | Plasmid Miniprep ]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/digest | Restriction Digest ]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/vector_dephos | Vector Dephosphorylation]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/ligation | Ligation ]]
 +
-----------------------------
 +
*[[Team:Alberta/Notebook/protocols/agarose_gel | Agarose Gel Electrophoresis ]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/gel_extraction | Gel Extraction ]]
 +
-----------------------------
 +
*[[Team:Alberta/Notebook/protocols/pcr | PCR]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/colony_pcr | Colony PCR ]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/pcr_purification | PCR Purification ]]
 +
-----------------------------
 +
*[[Team:Alberta/Notebook/protocols/labelling | Sample Labelling Conventions]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/sequencing | Fluorescent Sequencing Reaction]]
 +
 
 +
*[[Team:Alberta/Notebook/protocols/primer_design | Primer Design]]
 +
-----------------------------
 +
 
 +
 
 +
{{Team:Alberta/endMainContent}}
 +
 
-
{{Team:Alberta/beginLeftSideBar}}
 
{{Team:Alberta/endLeftSideBar}}
{{Team:Alberta/endLeftSideBar}}

Latest revision as of 02:29, 27 October 2010

TEAM ALBERTA


Gel Extraction

Reagents:

  • Clean scalpel
  • Transilluminator
  • Eppendorf tube
  • Gel extraction kit
  • 55oC water bath
  • Vortex
  • Microfuge


Procedure:

  • Place the gel on the transilluminator. Put on face shield and gloves.
  • Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
  • Pry out the gel ‘cube’ and place it in an eppendorf tube.
  • Follow instructions on pp. 25 of QIAQuick Spin Handbook.