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Team:Alberta/Notebook/TransformingCells
From 2010.igem.org
Transformation typically requires expensive equipment and storing competent cells is difficult for many high schools who do not have -80C freezers. We tried a few methods of transforming cells and making them competent to attempt to solve this problem in our kit.
TSS Transformation Protocol
The TSS transformation was tested. The procedure is listed here.
One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution
http://www.pnas.org/content/86/7/2172.abstract
Acquistion of reagents- June 3, 2010 Preparation of buffer - June 7, 2010 Cell preparation - June 7, 2010 Competency - June 10, 2010
Rubidium chloride competent cells and lyophilization
This method was adapted from a method used by Justin Fedor.
Preparation of transformation buffer - July 6, 2010 Preparation of cells - July 10, 2010 Preparing competent cells - July 11, 2010 Lyophilization - July 13, 2010 Transformation of FD cells - July 14, 2010 Preparation of cells 2 - July 30, 2010 Lyophilization - August 2, 2010 Transformation - August 5, 2010
