Team:Alberta/Kit components

From 2010.igem.org

(Difference between revisions)
Line 26: Line 26:
! Resistance Marker
! Resistance Marker
|-
|-
-
| Green Fluorescent Protein
+
| GFP
| CrtE
| CrtE
| VioA
| VioA
Line 32: Line 32:
| Ampicillin
| Ampicillin
|-
|-
-
| mRed Fluorescent Protein
+
| mRFP
| CrtB
| CrtB
| VioB
| VioB
Line 38: Line 38:
|Chloramphenicol
|Chloramphenicol
|-
|-
-
| Cyan Fluorescent Protein
+
| CFP
| CrtI
| CrtI
| VioD
| VioD
Line 44: Line 44:
| Kanamycin
| Kanamycin
|-
|-
-
| Yellow Fluorescent Protein
+
| YFP
| CrtY
| CrtY
| VioE
| VioE

Revision as of 02:23, 27 October 2010

TEAM ALBERTA

The DNA Components

Open Reading Frame Parts

Fluorescent Proteins Carotenoid Pathway Violacein Pathway Transcription Factors Resistance Marker
GFP CrtE VioA LacI Ampicillin
mRFP CrtB VioB AraC Chloramphenicol
CFP CrtI VioD LambdaCI Kanamycin
YFP CrtY VioE TetR Tetramycin


Linker Parts
GENOMIKON's Parts
  • Transcription
  • 5 Anderson Promoters
  • Operon Linker
  • Anti-linker as a negative control
  • Short null-linker for inversion of ends
  • Fusion Protein Link

Other Parts

  • High, medium and low copy number Oris
  • Caps
  • Anchors

Other Components

These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON.

  • Precision Droppers to accurately dispense small volumes of liquids
  • Magnetic Racks
  • Eppendorf Tubes
  • Wash Buffer
  • Elution Buffer
  • Lyophilized Competent Cells
  • LB media
  • Plates
  • Iron Micro Beads