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Team:Alberta/Kit components
From 2010.igem.org
(Difference between revisions)
| Line 32: | Line 32: | ||
| Ampicillin | | Ampicillin | ||
|- | |- | ||
| - | | | + | | mRed Fluorescent Protein |
| CrtB | | CrtB | ||
| VioB | | VioB | ||
| Line 55: | Line 55: | ||
'''Linker Parts''' | '''Linker Parts''' | ||
*Transcription | *Transcription | ||
| + | *5 Anderson Promoters | ||
| + | *Operon Linker | ||
| + | *Anti-linker as a negative control | ||
| + | *Short null-linker for inversion of ends | ||
| + | *Fusion Protein Link | ||
'''Other Parts''' | '''Other Parts''' | ||
| - | + | *High, medium and low copy number Oris | |
| + | *Caps | ||
| + | *Anchors | ||
==Other Components== | ==Other Components== | ||
<div id="horiz-line"></div> | <div id="horiz-line"></div> | ||
| Line 68: | Line 75: | ||
*Elution Buffer | *Elution Buffer | ||
*Lyophilized Competent Cells | *Lyophilized Competent Cells | ||
| - | * | + | *LB media |
*Plates | *Plates | ||
*Iron Micro Beads | *Iron Micro Beads | ||
Revision as of 02:20, 27 October 2010
The DNA Components
Open Reading Frame Parts
| Fluorescent Proteins | Carotenoid Pathway | Violacein Pathway | Transcription Factors | Resistance Marker |
|---|---|---|---|---|
| Green Fluorescent Protein | CrtE | VioA | LacI | Ampicillin |
| mRed Fluorescent Protein | CrtB | VioB | AraC | Chloramphenicol |
| Cyan Fluorescent Protein | CrtI | VioD | LambdaCI | Kanamycin |
| Yellow Fluorescent Protein | CrtY | VioE | TetR | Tetramycin |
Linker Parts
- Transcription
- 5 Anderson Promoters
- Operon Linker
- Anti-linker as a negative control
- Short null-linker for inversion of ends
- Fusion Protein Link
Other Parts
- High, medium and low copy number Oris
- Caps
- Anchors
Other Components
These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON.
- Precision Droppers to accurately dispense small volumes of liquids
- Magnetic Racks
- Eppendorf Tubes
- Wash Buffer
- Elution Buffer
- Lyophilized Competent Cells
- LB media
- Plates
- Iron Micro Beads
