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Team:Alberta/Kit
From 2010.igem.org
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GENOMIKON evolved from a team objective to bring synthetic biology to high school students. This proved to be a tall task as a high school environment has many limitations that don't apply to university research labs. </p> | GENOMIKON evolved from a team objective to bring synthetic biology to high school students. This proved to be a tall task as a high school environment has many limitations that don't apply to university research labs. </p> | ||
<p>To work GENOMIKON must be: | <p>To work GENOMIKON must be: | ||
| - | |||
*Require very little expensive equipment (autoclaves, centrifuges, -70C freezers) | *Require very little expensive equipment (autoclaves, centrifuges, -70C freezers) | ||
*Fast | *Fast | ||
*Efficicent | *Efficicent | ||
*Easily shipped | *Easily shipped | ||
| + | *Inexpensive | ||
</p> | </p> | ||
'''The Innovation'''<p> | '''The Innovation'''<p> | ||
| - | The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, [[Team:Alberta/BioByte 2 |BioBytes 2.0]] | + | The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, [[Team:Alberta/BioByte 2 |BioBytes 2.0]]. The BioByte 2.0 standard solved most of the problems.</p><p>BioBytes 2.0 is: |
| + | *Fast-Parts can be added to a growing construct in 7 minutes. | ||
| + | *Efficient-It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into ''E. coli''. | ||
| + | *Equipment-It does not require centrifugation or any other expensive equipment inherently. | ||
</p> | </p> | ||
| + | '''The Kit'''<p> | ||
| + | The other limitations were solved by using innovative protocols and products: | ||
| + | *Equipment-Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions. | ||
| + | *Easily Shipped-We will utilize lyophilized competent cells so that cold temperatures are not neccesary. | ||
| + | *Inexpensive-Based on our [[Team:Alberta/Distribution analysis|distribution analysis]] the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs. | ||
| + | '''[picture of kit buddi]''' | ||
| + | |||
| + | </p> | ||
{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} | ||
Revision as of 02:16, 26 October 2010
GENOMIKON
The Idea
GENOMIKON evolved from a team objective to bring synthetic biology to high school students. This proved to be a tall task as a high school environment has many limitations that don't apply to university research labs.
To work GENOMIKON must be:
- Require very little expensive equipment (autoclaves, centrifuges, -70C freezers)
- Fast
- Efficicent
- Easily shipped
- Inexpensive
The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, BioBytes 2.0. The BioByte 2.0 standard solved most of the problems.
BioBytes 2.0 is:
- Fast-Parts can be added to a growing construct in 7 minutes.
- Efficient-It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into E. coli.
- Equipment-It does not require centrifugation or any other expensive equipment inherently.
The other limitations were solved by using innovative protocols and products:
- Equipment-Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
- Easily Shipped-We will utilize lyophilized competent cells so that cold temperatures are not neccesary.
- Inexpensive-Based on our distribution analysis the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.
