http://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&feed=atom&action=historyTeam:Aberdeen Scotland - Revision history2024-03-29T08:59:43ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=193592&oldid=prevBrychan at 19:31, 27 October 20102010-10-27T19:31:56Z<p></p>
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</table>Brychanhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=182056&oldid=prevSlam at 13:12, 27 October 20102010-10-27T13:12:15Z<p></p>
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</table>Slamhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=181765&oldid=prevSlam at 13:01, 27 October 20102010-10-27T13:01:18Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Over the course of the summer, the University of Aberdeen iGEM team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters. The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential. These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Over the course of the summer, the University of Aberdeen iGEM team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters. The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential. These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.</div></td></tr>
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</table>Slamhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=158668&oldid=prevPorter at 12:11, 26 October 20102010-10-26T12:11:11Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h1>Why <del class="diffchange diffchange-inline">AyeSwitch</del>?</h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h1>Why <ins class="diffchange diffchange-inline">ayeSwitch</ins>?</h1></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Over the course of the summer, the University of Aberdeen iGEM team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters. The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential. These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Over the course of the summer, the University of Aberdeen iGEM team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters. The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential. These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.</div></td></tr>
</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=150022&oldid=prevPorter at 21:53, 25 October 20102010-10-25T21:53:01Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Over the course of the summer, the University of Aberdeen <del class="diffchange diffchange-inline">iGem </del>team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters. The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential. These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Over the course of the summer, the University of Aberdeen <ins class="diffchange diffchange-inline">iGEM </ins>team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters. The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential. These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.</div></td></tr>
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</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=138598&oldid=prevPorter at 23:01, 24 October 20102010-10-24T23:01:00Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:Aberdeen_Scotland/Project_Overview">Your ayeSwitch experience begins here<del class="diffchange diffchange-inline">;</del>&nbsp;<img src="https://static.igem.org/mediawiki/2010/3/36/Right_arrow.png"></a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:Aberdeen_Scotland/Project_Overview">Your ayeSwitch experience begins here&nbsp;<img src="https://static.igem.org/mediawiki/2010/3/36/Right_arrow.png"></a></div></td></tr>
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</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=138029&oldid=prevPorter at 22:04, 24 October 20102010-10-24T22:04:39Z<p></p>
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</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=134036&oldid=prevPorter at 16:17, 24 October 20102010-10-24T16:17:11Z<p></p>
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</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=126998&oldid=prevPorter at 19:47, 23 October 20102010-10-23T19:47:35Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h1>AyeSwitch <del class="diffchange diffchange-inline">- project abstract</del></h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h1><ins class="diffchange diffchange-inline">Why </ins>AyeSwitch<ins class="diffchange diffchange-inline">?</ins></h1></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Over the course of the summer, the University of Aberdeen iGem team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters. The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential. These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Over the course of the summer, the University of Aberdeen iGem team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters. The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential. These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.</div></td></tr>
</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland&diff=126993&oldid=prevPorter at 19:47, 23 October 20102010-10-23T19:47:05Z<p></p>
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