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Talk:Team:IvyTech-South Bend/Notebook/21 September 2010
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Revision as of 19:26, 21 September 2010 by Rchamberlin (Talk | contribs)
Electroporation of AT BBa_T9002
thawed electro comp cells from -80C freezer
set up 4 1.5 ul sterile centrifuge tubes with 90 ul SOB
two electro porations with at , one with 20ul and one with 3 ul BBa_T9002
chilled cuvette ince bath
reading for 20ul was 2.2kr @ 0.70ms
removed with 1ul SOB media
placed into 1.5 ml sterile centrifuge tube at room tem for 3 hour Prof T will streak
--Rchamberlin Jhull 19:25, 21 September 2010 (UTC)
