Talk:Team:IvyTech-South Bend/5 October 2010

From 2010.igem.org

Latest revision as of 16:48, 27 October 2010

10/5/2010

pTet GFP

Untagged GFP behind a constitutive promoter. Usage and Biology

GFP only control Spring 2010 Distribution

2010 Kit Plate 2 8A pSB1A2

Part:BBa_I732017

Extracting DNA from I732017 using protocol from p 19 after extraction placing in freezer.

I13511

H.Y Electroporated a part I13511 with E-Coli,

After waiting 45 min, I streaked on an Amp/Agar plate and placed into incubator @ 37C overnight placing the rest of the spl. in incubator @ 37C.

--for JHull 5 October 2010 Rchamberlin 20:10, 17 October 2010 (UTC)

10/5/10

Suspension of A.tumefaciens and A.T9002 in LB amp

I’m suspending Agro Tumefaciens +T9002 in 20 ml of LB/Broth w/ 20 ul of Amp. in the A.T9002 suspension only 1- first I poured 20 ml of LB Broth (prepared by JH) into 6 - .45 ml steril tubes

2- I then added 20 ul of Amp into the tube, (only one containing Agro T9002) I pulled a sample of Agro + T9002 from a colony and suspended it into my LB/Amp Broth (plate streaked by IF)

3- I pulled cells from the colony A.Tume. and suspended them in LB Broth (20 ml)

4- Next, I pulled cells from a colony B.Thurlgensis (made by AM) and suspended in 20 ml of LB Broth

5- I pulled cells from plate containing B. Magetarium and suspended in Broth. B. Magetarium made by AM

6- I pulled cells from the plate containing S.Lactis(prepared by AM) suspended in 20ml of LB broth

7- I pulled cells from the Mycobacterium plate (prepared by AM) and suspended in 20 ml – I performed this in a sterile hood.

8- each time of suspension, I sterilized my loop before and after pulling cells from plates.

9- I’m going to leave tubes and cells on a shaker over night to stimulate growth, then I will restreak all cells except for the Agro T9002 cells.

--for DGarvey 5 October 2010 Rchamberlin 16:41, 27 October 2010 (UTC)