http://2010.igem.org/wiki/index.php?title=Special:Contributions/Pmboyle&feed=atom&limit=50&target=Pmboyle&year=&month=2010.igem.org - User contributions [en]2024-03-29T02:16:42ZFrom 2010.igem.orgMediaWiki 1.16.5http://2010.igem.org/Team:Harvard/safetyTeam:Harvard/safety2010-10-27T19:23:55Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<html><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<br><br />
<h1>safety</h1><br />
<br />
<p>To address potential safety issues with our project, we were asked by iGEM to answer the following questions:</p><br />
<table border="0"><br />
<tr><br />
<td valign=Top> <br />
<ol><br />
<li><strong>Would any of your project ideas raise safety issues in terms of:<br />
</strong><br />
<ul><br />
<li> researcher safety, </li><br />
<li> public safety, or </li><br />
<li> environmental safety? </li><br />
</ul><br />
<br><br />
<p>In terms of researcher and public safety, all research was performed in Biosafety Level 1 (BSL1) facilities, following all local and federal regulations with regards to BSL1 research. Any version of the iGarden toolkit meant for use by the general public would require additional precautions to prevent spread of genetically modified plants into the environment. To this end, we have considered <a href="https://2010.igem.org/Team:Harvard/fences/safety">best practices for public safety</a> and decided that any finalized toolkit would require a robust <a <br />
href="https://2010.igem.org/Team:Harvard/fences">Genetic Fence</a>.</p><br />
<br><br />
<br><br />
</li><br />
<li><strong> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
</strong><br />
<ul><br />
<li> did you document these issues in the Registry? </li><br />
<li> how did you manage to handle the safety issue? </li><br />
<li> How could other teams learn from your experience? </li><br />
</ul><br />
<br><br />
<p>None of our parts raise any safety issues, but we recommend that any groups that intend to utilize our parts and devices follow established guidelines for the research of transgenic plants. For our project, all arabidopsis research was performed indoors in a BSL1 laboratory setting. In the future, we would like to deploy the iGarden kit to a wider audience, but that will require the inclusion of the completed genetic fence, along with regulatory approval.</p><br />
<p>To address concerns with the use of the Barnase enzyme for the Genetic Fence, we have put together a separate page on the safety of this device: <a href="https://2010.igem.org/Team:Harvard/fences/safety">Genetic Fence Safety</a>.<br />
<br><br />
<br><br />
</li><br />
<li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong><br />
<ul><br />
<li> If yes, what does your local biosafety group think about your project? </li><br />
<li> If no, which specific biosafety rules or guidelines do you have to consider in your country? </li><br />
</ul><br />
</li><br />
<br />
<br><br />
<p>The <a href="http://www.uos.harvard.edu/ehs/">Harvard University Operations Services (UOS) Environmental Health and Safety Department</a> has guidelines primarily focused on minimizing exposure to and properly disposing of biohazardous materials. Although we have not consulted these groups directly regarding specific aspects of our project, our work involves standard cloning techniques that are relevant to the safety guidelines already in place at Harvard. All team members who worked at the bench this summer have had proper safety training. Guidelines were strictly enforced by the Teaching Fellows.</p><br><br />
<br />
<li><strong>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong></li><br />
<br><br />
<br />
<p>The Registry of Standard Biological Parts could compose a set of safety regulations (e.g., proper disposal, safe lab practices, etc.) and a contract that users are required to read and agree with before receiving parts from the registry. Simple practices to prevent the spread of potentially harmful substances into the environment is an inexpensive practical alternative to complicated biosafety engineering; this approach has been effective over the past several decades of molecular cloning.</p><br />
<br />
<br />
<br />
<br />
</ol></td><br />
<td><p>&nbsp;</p> </td><br />
</tr><br />
</table><br />
</div><br />
<br />
<br />
<!-- Hidden content<br />
<br />
<div id="maincontent"><br />
<div id="abstract"><br />
<br />
<table border="0"><br />
<tr><br />
<td valign=Top bgcolor="#CCCCCC"> <br />
<ol><br />
<li>In terms of researcher and public safety, all research was performed in Biosafety Level 1 (BSL1) facilities, following all local and federal regulations with regards to BSL1 research. Any version of the iGarden toolkit meant for use by the general public would require additional precautions to prevent spread of genetically modified plants into the environment. To this end, we decided that any finalized toolkit would require a robust <a <br />
href="https://2010.igem.org/Team:Harvard/fences">Genetic Fence</a>.<br />
</li><br />
<li>We recommend that any groups that intend to utilize our parts and devices follow established guidelines for the research of transgenic plants. For our project, all arabidopsis research was performed indoors in a BSL1 laboratory setting. All parts in agrobacterium integration vectors submitted to the registry will be annotated as such, with the recommendation that they only be integrated into plants by researchers familiar with plant genetic techniques. In the future, we would like to deploy the iGarden kit to a wider audience, but that will require the inclusion of the completed genetic fence, along with regulatory approval.<br />
</li><br />
<li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong><br />
<ul><br />
<li> If yes, what does your local biosafety group think about your project? </li><br />
<li> If no, which specific biosafety rules or guidelines do you have to consider in your country? </li><br />
</ul><br />
</li><br />
<li><strong>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong></li><br />
</ol></td><br />
<td><p>&nbsp;</p> </td><br />
</tr><br />
</table><br />
</div><br />
<br />
end of hidden content --></div>Pmboylehttp://2010.igem.org/Team:Harvard/safetyTeam:Harvard/safety2010-10-27T19:17:43Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<html><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<br><br />
<h1>safety</h1><br />
<br />
<p>To address potential safety issues with our project, we were asked by iGEM to answer the following questions:</p><br />
<table border="0"><br />
<tr><br />
<td valign=Top> <br />
<ol><br />
<li><strong>Would any of your project ideas raise safety issues in terms of:<br />
</strong><br />
<ul><br />
<li> researcher safety, </li><br />
<li> public safety, or </li><br />
<li> environmental safety? </li><br />
</ul><br />
<br><br />
<p>In terms of researcher and public safety, all research was performed in Biosafety Level 1 (BSL1) facilities, following all local and federal regulations with regards to BSL1 research. Any version of the iGarden toolkit meant for use by the general public would require additional precautions to prevent spread of genetically modified plants into the environment. To this end, we have considered <a href="https://2010.igem.org/Team:Harvard/fences/safety">best practices for public safety</a> and decided that any finalized toolkit would require a robust <a <br />
href="https://2010.igem.org/Team:Harvard/fences">Genetic Fence</a>.</p><br />
<br><br />
<br><br />
</li><br />
<li><strong> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
</strong><br />
<ul><br />
<li> did you document these issues in the Registry? </li><br />
<li> how did you manage to handle the safety issue? </li><br />
<li> How could other teams learn from your experience? </li><br />
</ul><br />
<br><br />
<p>None of our parts raise any safety issues, but we recommend that any groups that intend to utilize our parts and devices follow established guidelines for the research of transgenic plants. For our project, all arabidopsis research was performed indoors in a BSL1 laboratory setting. In the future, we would like to deploy the iGarden kit to a wider audience, but that will require the inclusion of the completed genetic fence, along with regulatory approval.</p><br />
<br><br />
<br><br />
</li><br />
<li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong><br />
<ul><br />
<li> If yes, what does your local biosafety group think about your project? </li><br />
<li> If no, which specific biosafety rules or guidelines do you have to consider in your country? </li><br />
</ul><br />
</li><br />
<br />
<br><br />
<p>The <a href="http://www.uos.harvard.edu/ehs/">Harvard University Operations Services (UOS) Environmental Health and Safety Department</a> has guidelines primarily focused on minimizing exposure to and properly disposing of biohazardous materials. Although we have not consulted these groups directly regarding specific aspects of our project, our work involves standard cloning techniques that are relevant to the safety guidelines already in place at Harvard. All team members who worked at the bench this summer have had proper safety training. Guidelines were strictly enforced by the Teaching Fellows.</p><br><br />
<br />
<li><strong>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong></li><br />
<br><br />
<br />
<p>The Registry of Standard Biological Parts could compose a set of safety regulations (e.g., proper disposal, safe lab practices, etc.) and a contract that users are required to read and agree with before receiving parts from the registry. Simple practices to prevent the spread of potentially harmful substances into the environment is an inexpensive practical alternative to complicated biosafety engineering; this approach has been effective over the past several decades of molecular cloning.</p><br />
<br />
<br />
<br />
<br />
</ol></td><br />
<td><p>&nbsp;</p> </td><br />
</tr><br />
</table><br />
</div><br />
<br />
<br />
<!-- Hidden content<br />
<br />
<div id="maincontent"><br />
<div id="abstract"><br />
<br />
<table border="0"><br />
<tr><br />
<td valign=Top bgcolor="#CCCCCC"> <br />
<ol><br />
<li>In terms of researcher and public safety, all research was performed in Biosafety Level 1 (BSL1) facilities, following all local and federal regulations with regards to BSL1 research. Any version of the iGarden toolkit meant for use by the general public would require additional precautions to prevent spread of genetically modified plants into the environment. To this end, we decided that any finalized toolkit would require a robust <a <br />
href="https://2010.igem.org/Team:Harvard/fences">Genetic Fence</a>.<br />
</li><br />
<li>We recommend that any groups that intend to utilize our parts and devices follow established guidelines for the research of transgenic plants. For our project, all arabidopsis research was performed indoors in a BSL1 laboratory setting. All parts in agrobacterium integration vectors submitted to the registry will be annotated as such, with the recommendation that they only be integrated into plants by researchers familiar with plant genetic techniques. In the future, we would like to deploy the iGarden kit to a wider audience, but that will require the inclusion of the completed genetic fence, along with regulatory approval.<br />
</li><br />
<li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong><br />
<ul><br />
<li> If yes, what does your local biosafety group think about your project? </li><br />
<li> If no, which specific biosafety rules or guidelines do you have to consider in your country? </li><br />
</ul><br />
</li><br />
<li><strong>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong></li><br />
</ol></td><br />
<td><p>&nbsp;</p> </td><br />
</tr><br />
</table><br />
</div><br />
<br />
end of hidden content --></div>Pmboylehttp://2010.igem.org/Team:Harvard/safetyTeam:Harvard/safety2010-10-27T19:11:42Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<html><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<br><br />
<h1>safety</h1><br />
<br />
<p>To address potential safety issues with our project, we were asked by iGEM to answer the following questions:</p><br />
<table border="0"><br />
<tr><br />
<td valign=Top> <br />
<ol><br />
<li><strong>Would any of your project ideas raise safety issues in terms of:<br />
</strong><br />
<ul><br />
<li> researcher safety, </li><br />
<li> public safety, or </li><br />
<li> environmental safety? </li><br />
</ul><br />
<br><br />
<p>In terms of researcher and public safety, all research was performed in Biosafety Level 1 (BSL1) facilities, following all local and federal regulations with regards to BSL1 research. Any version of the iGarden toolkit meant for use by the general public would require additional precautions to prevent spread of genetically modified plants into the environment. To this end, we have considered <a href="https://2010.igem.org/Team:Harvard/fences/safety">best practices for public safety</a> and decided that any finalized toolkit would require a robust <a <br />
href="https://2010.igem.org/Team:Harvard/fences">Genetic Fence</a>.</p><br />
<br><br />
<br><br />
</li><br />
<li><strong> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
</strong><br />
<ul><br />
<li> did you document these issues in the Registry? </li><br />
<li> how did you manage to handle the safety issue? </li><br />
<li> How could other teams learn from your experience? </li><br />
</ul><br />
<br><br />
<p>We recommend that any groups that intend to utilize our parts and devices follow established guidelines for the research of transgenic plants. For our project, all arabidopsis research was performed indoors in a BSL1 laboratory setting. In the future, we would like to deploy the iGarden kit to a wider audience, but that will require the inclusion of the completed genetic fence, along with regulatory approval.</p><br />
<br><br />
<br><br />
</li><br />
<li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong><br />
<ul><br />
<li> If yes, what does your local biosafety group think about your project? </li><br />
<li> If no, which specific biosafety rules or guidelines do you have to consider in your country? </li><br />
</ul><br />
</li><br />
<br />
<br><br />
<p>The <a href="http://www.uos.harvard.edu/ehs/">Harvard University Operations Services (UOS) Environmental Health and Safety Department</a> has guidelines primarily focused on minimizing exposure to and properly disposing of biohazardous materials. Although we have not consulted these groups directly regarding specific aspects of our project, our work involves standard cloning techniques that are relevant to the safety guidelines already in place at Harvard. All team members who worked at the bench this summer have had proper safety training. Guidelines were strictly enforced by the Teaching Fellows.</p><br><br />
<br />
<li><strong>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong></li><br />
<br><br />
<br />
<p>The Registry of Standard Biological Parts could compose a set of safety regulations (e.g., proper disposal, safe lab practices, etc.) and a contract that users are required to read and agree with before receiving parts from the registry. Simple practices to prevent the spread of potentially harmful substances into the environment is an inexpensive practical alternative to complicated biosafety engineering; this approach has been effective over the past several decades of molecular cloning.</p><br />
<br />
<br />
<br />
<br />
</ol></td><br />
<td><p>&nbsp;</p> </td><br />
</tr><br />
</table><br />
</div><br />
<br />
<br />
<!-- Hidden content<br />
<br />
<div id="maincontent"><br />
<div id="abstract"><br />
<br />
<table border="0"><br />
<tr><br />
<td valign=Top bgcolor="#CCCCCC"> <br />
<ol><br />
<li>In terms of researcher and public safety, all research was performed in Biosafety Level 1 (BSL1) facilities, following all local and federal regulations with regards to BSL1 research. Any version of the iGarden toolkit meant for use by the general public would require additional precautions to prevent spread of genetically modified plants into the environment. To this end, we decided that any finalized toolkit would require a robust <a <br />
href="https://2010.igem.org/Team:Harvard/fences">Genetic Fence</a>.<br />
</li><br />
<li>We recommend that any groups that intend to utilize our parts and devices follow established guidelines for the research of transgenic plants. For our project, all arabidopsis research was performed indoors in a BSL1 laboratory setting. All parts in agrobacterium integration vectors submitted to the registry will be annotated as such, with the recommendation that they only be integrated into plants by researchers familiar with plant genetic techniques. In the future, we would like to deploy the iGarden kit to a wider audience, but that will require the inclusion of the completed genetic fence, along with regulatory approval.<br />
</li><br />
<li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong><br />
<ul><br />
<li> If yes, what does your local biosafety group think about your project? </li><br />
<li> If no, which specific biosafety rules or guidelines do you have to consider in your country? </li><br />
</ul><br />
</li><br />
<li><strong>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong></li><br />
</ol></td><br />
<td><p>&nbsp;</p> </td><br />
</tr><br />
</table><br />
</div><br />
<br />
end of hidden content --></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T19:04:31Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<a href="http://www.huh.harvard.edu/research/mathews-lab/">the mathews lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Sub-projects were planned and executed by the following team members:</p><br />
<br><br />
<ul><b>Plant Vectors and Color BioBricks</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Allergy Knock-down BioBricks</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Flavor BioBricks</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Genetic Fence Circuit</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p><br />
<br><br />
<p>Web design was led by Jackie Quinn and Aaron Deardon.</p><br />
<br><br />
<p>Virtual iGarden was created by Aaron Deardon.</p><br />
<br><br />
<p>The iGarden box was constructed by Jonathan deWerd and Oliver Medvedik.</p><br />
<br><br />
<p>Survey and human practices research was performed by the entire team and survey data was analyzed by Lu Wang.</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:59:41Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<a href="http://www.huh.harvard.edu/research/mathews-lab/">the mathews lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Sub-projects were planned and executed by the following team members:</p><br />
<br><br />
<ul><b>Plant Vectors and Color BioBricks</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Allergy Knock-down BioBricks</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Flavor BioBricks</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Genetic Fence Circuit</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p><br />
<br><br />
<p>Web design was led by Jackie Quinn and Aaron Deardon.</p><br />
<br><br />
<p>Virtual iGarden was created by Aaron Deardon.</p><br />
<br><br />
<p>The iGarden box was constructed by Jonathan deWerd and Oliver Medvedik.</p><br />
<br><br />
<p>Survey and human practices research was performed by the entire team and analyzed by <br><br />
Lu Wang.</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:57:17Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<a href="http://www.huh.harvard.edu/research/mathews-lab/">the mathews lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Sub-projects were planned and executed by the following team members:</p><br />
<br><br />
<ul><b>Plant Vectors and Color BioBricks</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Allergy Knock-down BioBricks</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Flavor BioBricks</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Genetic Fence Circuit</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p><br />
<br><br />
<p>Web design was led by Jackie Quinn and Aaron Deardon.</p><br />
<br><br />
<p>Virtual iGarden was created by Aaron Deardon</p><br />
<br><br />
<p>The iGarden box was constructed by Jonathan deWerd and Oliver Medvedik.</p><br />
<br><br />
<p>Survey and human practices research was performed by the entire team and analyzed by <br><br />
Lu Wang</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:56:46Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<a href="http://www.huh.harvard.edu/research/mathews-lab/">the mathews lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Sub-projects were planned and executed by the following team members:</p><br />
<br><br />
<ul><b>Plant Vectors and Color BioBricks</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Allergy Knock-down BioBricks</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Flavor BioBricks</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Genetic Fence Circuit</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p><br />
<br><br />
<p>Web design was led by Jackie Quinn and Aaron Deardon.</p><br />
<br><br />
<p>Virtual iGarden was created by Aaron Deardon</p><br />
<br><br />
<p>The iGarden box was constructed by Jonathan deWerd and Oliver Medvedik.</p><br />
<br><br />
<p>Survey and human practices research was performed by the entire team and analyzed by Lu Wang</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:55:58Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<a href="http://www.huh.harvard.edu/research/mathews-lab/">the mathews lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Sub-projects were planned and executed by the following team members:</p><br />
<br><br />
<ul><b>Plant Vectors and Color BioBricks</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Allergy Knock-down BioBricks</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Flavor BioBricks</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Genetic Fence Circuit</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p><br />
<br><br />
<p>Web design was led by Jackie Quinn and Aaron Deardon.</p><br />
<br><br />
<p>Virtual iGarden was created by Aaron Deardon</p><br />
<br><br />
<p>The iGarden box was constructed by Jonathan deWerd and Oliver Medvedik.</p><br />
<br><br />
<p>Survey and human practices research was performed by the entire team.</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:54:28Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<a href="http://www.huh.harvard.edu/research/mathews-lab/">the mathews lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Sub-projects were planned and executed by the following team members:</p><br />
<br><br />
<ul><b>Plant Vectors and Color BioBricks</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Allergy Knock-down BioBricks</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Flavor BioBricks</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Genetic Fence Circuit</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p><br />
<br><br />
<p>Web design was led by Jackie Quinn and Aaron Deardon.</p><br />
<br><br />
<p>Virtual iGarden was created by Aaron Deardon</p><br />
<br><br />
<p>The iGarden box was constructed by Jonathan deWerd and Oliver Medvedik.</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:54:04Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<br><br />
<a href="http://www.huh.harvard.edu/research/mathews-lab/">the mathews lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Sub-projects were planned and executed by the following team members:</p><br />
<br><br />
<ul><b>Plant Vectors and Color BioBricks</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Allergy Knock-down BioBricks</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Flavor BioBricks</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Genetic Fence Circuit</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p><br />
<br><br />
<p>Web design was led by Jackie Quinn and Aaron Deardon.</p><br />
<br><br />
<p>Virtual iGarden was created by Aaron Deardon</p><br />
<br><br />
<p>The iGarden box was constructed by Jonathan deWerd and Oliver Medvedik.</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:45:40Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Sub-projects were designed and executed by the following team members:</p><br />
<br><br />
<ul><b>Team Vector/Color</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Allergy</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Flavor</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Fence</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p><br />
<br><br />
<p>Web design was led by Jackie Quinn and Aaron Deardon.</p><br />
<br><br />
<p>The iGarden box was constructed by Jonathan deWerd and Oliver Medvedik.</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:40:32Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Each team member worked on a specific sub-team:</p><br />
<br><br />
<ul><b>Team Vector/Color</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Allergy</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Flavor</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Fence</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:39:58Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Each team member worked on a specific subteam:</p><br />
<br><br />
<ul><b>Team Vector/Color</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Allergy</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Flavor</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Fence</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br><br />
<p>Plant transformations were performed by members of each sub-team in conjunction with Kurt Schellenberg in both the Mathews and Silver labs.</p></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:38:13Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Each team member worked on a specific subteam:</p><br />
<br><br />
<ul><b>Team Vector/Color</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Allergy</b><br />
<li>Jonathan deWerd</li><br />
<li>Anu Raman</li><br />
<li>Lu Wang</li><br />
<li>Christina Agapakis [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Flavor</b><br />
<li>Alex Gedeon</li><br />
<li>Mark Theilmann</li><br />
<li>Patrick Boyle [TF]</li><br />
</ul><br />
<br><br />
<ul><b>Team Fence</b><br />
<li>Morgan Paull</li><br />
<li>Julia Winn</li><br />
<li>Oliver Medvedik [TF]</li><br />
</ul><br />
<br></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:34:26Z<p>Pmboyle: </p>
<hr />
<div>{{harvardmain}}<br />
<br />
<br />
<html><br />
<div id="sidebar"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9e/Harvard2010teamphoto.jpg" width="300px"><br />
</div><br />
<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Each team member worked on a specific subteam:</p><br />
<br><br />
<ul><b>Team Vector/Color</b><br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br></div>Pmboylehttp://2010.igem.org/Team:Harvard/creditsTeam:Harvard/credits2010-10-27T18:34:00Z<p>Pmboyle: </p>
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<div>{{harvardmain}}<br />
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<div id="maincontent"><br />
<div id="abstract"><br />
<h2>many thanks!</h2><br />
<p>Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and <a href="http://www.huh.harvard.edu/research/mathews-lab/">Sarah Mathews</a> for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of <a href="http://wmd.weigelworld.org/">artificial microRNAs,</a> <a href="http://www.pi.csiro.au/rnai/vectors.htm">CSIRO</a> for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and <a href="http://www.arabidopsis.org/">TAIR,</a> for the pORE vector series.</p><br />
<br><br />
<h2>sponsors and supporters</h2><br />
<a href="http://wyss.harvard.edu/"><img src="https://static.igem.org/mediawiki/2010/b/bc/Wyss_HarvardiGEM.png" height=45></a>&nbsp;<br />
<a href="http://www.hhmi.org/"><img src="https://static.igem.org/mediawiki/2010/1/17/HHMI_HarvardiGEM.png" height=40></a>&nbsp;<br />
<a href="http://www.arabidopsis.org/"><img src="https://static.igem.org/mediawiki/2010/8/8d/TAIR_HarvardiGEM.gif" height=55></a>&nbsp;<br />
<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/"><img src="https://static.igem.org/mediawiki/2010/4/47/MrGene_HarvardiGEM.gif" height=60</a>&nbsp;<br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/f/f0/GENEART_HarvardiGEM.gif" height=30></a><br /><br /><br />
the harvard undergrad biolabs<br /><br /><br />
<a href="http://silver.med.harvard.edu/">the silver lab</a><br /><br /><br />
<br><br />
<h2>images</h2><br />
<p>Images used on this site were taken by us or licensed under the Creative Commons Share-alike license by the following Flickr users:</p><br />
<ul><li><a href="http://www.flickr.com/photos/blueridgekitties/3822413363/">blueridgekitties</a></li><br />
<li><a href="http://www.flickr.com/photos/ambernussbaum/2467940016/">ambernussbaum</a></li><br />
<li><a href="http://www.flickr.com/photos/bored-now/2255535425/">bored-now</a></li><br />
<li><a href="http://www.flickr.com/photos/bigberto/2404525663/">bigberto</a></li><br />
<li><a href="http://www.flickr.com/photos/22863752@N06/2669689192/sizes/o/">di_the_huntress</a</li><br />
<li><a href="http://www.flickr.com/photos/red_devil/63813182/">SeenyaRita</a></li><br />
</ul><br />
<br><br />
<h2>attributions</h2><br />
<p>The iGarden project was conceived and designed by the 2010 Harvard iGEM Team. Each team member worked on a specific subteam:</p><br />
<br><br />
<ul>Team Vector/Color<br />
<li>Aaron Deardon</li><br />
<li>Jackie Quinn</li><br />
<li>Devin Burrill [TF]</li><br />
<li>Mara Inniss [TF]</li><br />
</ul><br />
<br></div>Pmboylehttp://2010.igem.org/Team:Harvard/fences/resultsTeam:Harvard/fences/results2010-10-27T18:09:10Z<p>Pmboyle: </p>
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<div>{{HarvardFancybox}}<br />
{{harvard_fence}}<br />
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<h2>induction of barnase (death gene) reduces cell growth</h2><br />
<br />
<p><br />
<p>We characterized the activity of Barnase on an inducible plasmid constructed by <a href="https://2007.igem.org/BerkiGEM2007Present5">UC Berkeley for iGEM 2007</a> (parts BBa_I716408C and BBa_I716211). This construct works by expressing background levels of Barstar with in the presence of an inducible Barnase. When induced, Barnase will overwhelm Barstar. Higher levels of Barnase expression resulted in lower rates of growth in the bacteria, affirming the principle of Barnase-based growth control for the genetic fence, and confirming the results from Berkeley 2008. We characterized the growth repression of Barnase under a range of arabinose inducer concentrations.<br><br><br />
Our results show that expression of Barnase is effective in reducing cell growth, suggesting that Barnase will enable the genetic fence to prevent growth of iGarden plants outside of their designated areas.<br />
</p><br />
<br><br />
<div><b>barnase growth control in <i>E. Coli</i></b> &nbsp; <a href="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" style="font-size:12px">[click to enlarge]</a></div><hr/><br />
<a href="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" id="single_image"><br />
<img src="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" width="600px" border=0><br />
</a> </div><br />
<br><br />
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</html></div>Pmboylehttp://2010.igem.org/Team:HarvardTeam:Harvard2010-10-27T15:30:28Z<p>Pmboyle: </p>
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<p>team updates<a href="http://twitter.com/harvardigem" id="twitter-link">follow us on twitter</a></p><br />
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statusHTML.push('<li><img src="https://static.igem.org/mediawiki/2010/1/11/Harvard2010leaficon.png"/><span>'+status+'</span> <a style="font-size:85%" href="http://twitter.com/'+username+'/statuses/'+twitters[i].id+'">'+relative_time(twitters[i].created_at)+'</a></li>');<br />
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</div><br />
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<div id="maincontent"><br />
<div id="abstract"><br />
<h1>an open source toolkit for plant engineering</h1><br />
<img src="https://static.igem.org/mediawiki/2010/1/15/Leaf_dark-green.png" width="190" style="float: left; margin-top: 10px; margin-right:40px"/><br />
<br />
<div style="width: 600px"><br />
<p>The Harvard iGarden is a venture into plant engineering. Our aim is to create a toolkit for the cultivation of a personalized garden containing features introduced through synthetic biology. In addition to a "genetic fence" designed to prevent the spread of introduced genetic material, we have developed three independent features to be included in this toolkit - inclusion of novel flavors, knockdown of plant allergens, and modification of petal color.</p><br />
<br />
<p>All parts are BioBrick compatible and introduced into plants through agrobacterium-mediated transformation, using existing plant vectors modified with the BioBrick multiple cloning site.</p><br />
<br />
<p>The Harvard iGarden, beyond being an application of the BioBrick system to plant engineering, is an effort to raise public awareness of synthetic biology, production of food, and how the two can intertwine. We envision the iGarden as a medium through which the non-scientist can see the power and potential of synthetic biology and apply it to everyday life. </p><br />
</div><br />
<div><br />
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<td colspan=6><h2>iGarden development</h2><br />
</tr><br />
<tr><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/vectors"><img class="icon" src="https://static.igem.org/mediawiki/2010/f/fe/Harvard2010vectorheading2.png"></a></td><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/allergy"><img class="icon" src="https://static.igem.org/mediawiki/2010/9/9f/Harvard2010allergyheading2.jpg"></a></td><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/flavor"><img class="icon" src="https://static.igem.org/mediawiki/2010/d/d2/Harvard2010flavorheading2.jpg"></a></td><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/color"><img class="icon" src="https://static.igem.org/mediawiki/2010/5/58/Harvard2010colorheading2.png"></a></td><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/fences"><img class="icon" src="https://static.igem.org/mediawiki/2010/e/ed/Harvard2010fenceheading2.jpg"></a></td><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/parts"><img class="icon" src="https://static.igem.org/mediawiki/2010/d/d7/Harvard2010partsheading2.jpg"></a></td><br />
</tr><br />
<tr><br />
<td>vectors</td><br />
<td>allergy</td><br />
<td>flavor</td><br />
<td>color</td><br />
<td>genetic fence</td><br />
<td>submitted parts</td><br />
</tr><br />
<tr style="height:20px"></tr><br />
<tr><br />
<td colspan=6><h2>beyond the bench</h2></td><br />
</tr><br />
<tr><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/human_practices"><img class="icon" src="https://static.igem.org/mediawiki/2010/f/f9/Harvard2010hpheading2.png"></a></td><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/human_practices/vision"><img class="icon" src="https://static.igem.org/mediawiki/2010/d/d7/EarthEYE.jpg" height="102px"></a></td><br />
<td><a class="iconlink" href="http://www.hcs.harvard.edu/usundergrad/test/test4.php"><img class="icon" src="https://static.igem.org/mediawiki/2010/5/5c/Harvard2010virtualheadingsmall.png"></a></td><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/recycling"><img class="icon" src="https://static.igem.org/mediawiki/2010/7/7a/Harvard2010recyclingheading.jpg"></a></td><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/gallery"><img class="icon" src="https://static.igem.org/mediawiki/2010/6/6f/Camera.tiff"></a></td><br />
<td><a class="iconlink" href="https://2010.igem.org/Team:Harvard/credits"><img class="icon" src="https://static.igem.org/mediawiki/2010/7/74/Nobelprizemedal.jpeg" height="102px"></a></td><br />
</tr><br />
<tr><br />
<td>human practices</td><br />
<td>our vision</td><br />
<td>virtual iGarden</td><br />
<td>recycling columns</td><br />
<td>pictures & video</td><br />
<td>credits</td><br />
</tr><br />
<br />
</table><br />
<br /><br /><br />
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</div><br />
</div><br />
<p><br />
<!-- !END HARVARD --><br />
</html></div>Pmboylehttp://2010.igem.org/File:Leaf_dark-green.pngFile:Leaf dark-green.png2010-10-27T15:29:35Z<p>Pmboyle: </p>
<hr />
<div></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:33:43Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
<br />
<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb">link</a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb">link</a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb">link</a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb">link</a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb">link</a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb">link</a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">link</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">link</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">link</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt">link</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">link</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">link</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href="http://partsregistry.org/Part:BBa_K382018">link</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">link</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f12">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt">link</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">link</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:29:40Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
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<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href="http://partsregistry.org/Part:BBa_K382018">link</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f12">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/File:LYC.txtFile:LYC.txt2010-10-27T02:28:27Z<p>Pmboyle: </p>
<hr />
<div></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:24:32Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
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<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href="http://partsregistry.org/Part:BBa_K382018">link</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f12">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/File:Beta-ophase.txtFile:Beta-ophase.txt2010-10-27T02:23:17Z<p>Pmboyle: </p>
<hr />
<div></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:20:52Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
<br />
<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href="http://partsregistry.org/Part:BBa_K382018">link</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f12">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/File:Lut2.txtFile:Lut2.txt2010-10-27T02:19:36Z<p>Pmboyle: </p>
<hr />
<div></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:16:56Z<p>Pmboyle: </p>
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<body><br />
<br />
<br><br><br />
<h1>parts</h1><br />
<br />
<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href="http://partsregistry.org/Part:BBa_K382018">link</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f12">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:13:28Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
<br />
<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href="http://partsregistry.org/Part:BBa_K382018">link</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f12">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:10:53Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
<br />
<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href="http://partsregistry.org/Part:BBa_K382018">link</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:10:31Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
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<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href="http://partsregistry.org/Part:BBa_K382018"></a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:08:13Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
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<table cellspacing="0" width="900"><br />
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<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href=</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:06:20Z<p>Pmboyle: </p>
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<br><br><br />
<h1>parts</h1><br />
<br />
<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href=</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T02:02:44Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
<br />
<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href=</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T01:50:16Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
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<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href=</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/Team:Harvard/partsTeam:Harvard/parts2010-10-27T01:42:56Z<p>Pmboyle: </p>
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<h1>parts</h1><br />
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<table cellspacing="0" width="900"><br />
<br />
<tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> <br />
<br />
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr><br />
<br />
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr><br />
<br />
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr><br />
<br />
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr><br />
<br />
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr><br />
<br />
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr><br />
<br />
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href=</a></td></tr><br />
<br />
<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<br />
<tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<br />
<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<br />
<tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>ampicillin</td><td>-</td><td><a href="">link</a></td></tr><br />
<br />
<tr><td>GF3</td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<br />
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<br />
<tr><td>GF5</td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<br />
<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>ampicillin</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<br />
<tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr><br />
<br />
<tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<br />
<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
<br />
<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr><br />
</table></div>Pmboylehttp://2010.igem.org/File:Enter_the_virtual_igarden.pngFile:Enter the virtual igarden.png2010-10-27T01:37:30Z<p>Pmboyle: uploaded a new version of "Image:Enter the virtual igarden.png"</p>
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<div></div>Pmboylehttp://2010.igem.org/Team:Harvard/fences/resultsTeam:Harvard/fences/results2010-10-27T01:24:54Z<p>Pmboyle: </p>
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<div>{{HarvardFancybox}}<br />
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<h2>Induction of Barnase (death gene) reduces cell growth</h2><br />
<br />
<p><br />
<p>We characterized the activity of Barnase on an inducible plasmid constructed by <a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present5">UC Berkeley for iGEM 2007</a> (part I716408C). This construct works by expressing background levels of Barstar with in the presence of an inducible Barnase. When induced, Barnase will overwhelm Barstar. Higher levels of Barnase expression resulted in lower rates of growth in the bacteria, affirming the principle of Barnase-based growth control for the genetic fence, and confirming the results from Berkeley 2008. We characterized the growth repression of Barnase under a range of arabinose inducer concentrations.<br><br><br />
Our results show that expression of Barnase is effective in reducing cell growth, suggesting that Barnase will enable the genetic fence to prevent growth of iGarden plants outside of their designated areas.<br />
</p><br />
<br><br />
<div><b>barnase growth control in <i>E. Coli</i></b> &nbsp; <a href="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" style="font-size:12px">[click to enlarge]</a></div><hr/><br />
<a href="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" id="single_image"><br />
<img src="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" width="600px" border=0><br />
</a> </div><br />
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</html></div>Pmboylehttp://2010.igem.org/Team:Harvard/fences/resultsTeam:Harvard/fences/results2010-10-27T01:22:28Z<p>Pmboyle: </p>
<hr />
<div>{{HarvardFancybox}}<br />
{{harvard_fence}}<br />
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<br />
<br />
<html><br />
<div id="abstract"><br />
<h2>Induction of Barnase (death gene) reduces cell growth</h2><br />
<br />
<p><br />
<p>This construct works by expressing background levels of Barstar with in the presence of an inducible Barnase. When induced, Barnase will overwhelm Barstar. Higher levels of Barnase expression resulted in lower rates of growth in the bacteria, affirming the principle of Barnase-based growth control for the genetic fence, and confirming the results from Berkeley 2008. We characterized the growth repression of Barnase under a range of arabinose inducer concentrations.<br><br><br />
Our results show that expression of Barnase is effective in reducing cell growth, suggesting that Barnase will enable the genetic fence to prevent growth of iGarden plants outside of their designated areas.<br />
</p><br />
<br><br />
<div><b>barnase growth control in <i>E. Coli</i></b> &nbsp; <a href="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" style="font-size:12px">[click to enlarge]</a></div><hr/><br />
<a href="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" id="single_image"><br />
<img src="http://openwetware.org/images/2/2b/M4-growth_curves-10-4-10.jpg" width="600px" border=0><br />
</a> </div><br />
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</html></div>Pmboylehttp://2010.igem.org/Template:Harvard_cssTemplate:Harvard css2010-10-27T01:16:29Z<p>Pmboyle: Undo revision 170366 by Pmboyle (Talk)</p>
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</html></div>Pmboylehttp://2010.igem.org/Template:Harvard_cssTemplate:Harvard css2010-10-27T01:16:15Z<p>Pmboyle: Undo revision 170379 by Pmboyle (Talk)</p>
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<div><html><br />
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</html></div>Pmboylehttp://2010.igem.org/Template:Harvard_cssTemplate:Harvard css2010-10-27T01:15:38Z<p>Pmboyle: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
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#allcontent {<br />
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}<br />
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.firstHeading {<br />
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<br />
background-image:none;<br />
background-position: center;<br />
background-repeat: no-repeat;<br />
background-attachment: fixed; <br />
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</html></div>Pmboylehttp://2010.igem.org/Team:Harvard/fences/partsTeam:Harvard/fences/parts2010-10-27T01:12:15Z<p>Pmboyle: </p>
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<div>{{harvard_fence}}<br />
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<h1>parts and primers</h1><br />
<h2>Parts</h2><br />
<table cellspacing="0"><br />
<tr><td><b>Name</b></td><td><b>Biobrick Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>Barnase</td><td><center>-</center></td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f1">link</a></td></tr><br />
<tr><td>Barstar</td><td><center>-</center></td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f2">link</a></td></tr><br />
<tr><td>LacIN</td><td>BBa_K382035</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<tr><td>RXRLc</td><td>BBa_K382031</td><td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<tr><td>RXRHm</td><td>BBa_K382036</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<tr><td>VP16</td><td><center>-</center></td><td> Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f6">link</a></td></tr><br />
<tr><td>Ecdysone Receptor</td><td>BBa_K382033</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<tr><td>Gal4 DNA binding domain</td><td><center>-</center></td><td> Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f8">link</a></td></tr><br />
</table><br />
<br />
<br><br />
<br />
<h2>Promoters</h2><br />
<table cellspacing="0"><br />
<tr><td><b>Name</b></td><td><b>Description</b></td><td>Active conditions</td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>pENT CUP</td><td> Constitutive Plant Promoter</td><td>Constitutive</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f10">link</a></td></tr><br />
<tr><td>5xGal UAS</td><td> 5 time repeat of Gal4 upstream activating sequence</td><td>On in presense of dimerized EcR-Gal and RXRLc-VP16</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f11">link</a></td></tr><br />
<tr><td>Act2lacO</td><td>Actin2 promoter with Lac Inhibitor responsive sites (Lac O sites</td><td>Constitutive, inhibited by LacIN</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f12">link</a></td></tr><br />
<br />
<br />
<br />
</table><br />
<br /><br /><br />
<br />
<h2>Primers</h2><br />
<table cellspacing="0"><br />
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr><br />
<tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr><br />
<tr><td>Exp2MutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CCT ACA CAC GGA ACT CCT GGA AAT TAA GAC CG-3'</td></tr><br />
<tr><td>Exp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CGG TCT TAA TTT CCA GGA ACT CCG TGT GTA GG-3'</td></tr><br />
<tr><td>Bam.Gal4.Fwd</td><td>Attaching BamH1 sites to Gal4 in preparation for yeast vector ligation</td><td>5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'</td></tr><br />
<tr><td>HmRXR.Hind.Rev</td><td>Attaching HindIII sites to HmRXR in preparation for yeast vector ligation</td><td>5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'</td></tr><br />
<tr><td>LcRXR.Hind.Rev</td><td>Attaching HindIII sites to LcRXR in preparation for yeast vector ligation</td><td>5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3'</td></tr><br />
<tr><td>VP16.Hind.Fwd</td><td>Attaching HindIII sites to VP16 in preparation for yeast vector ligation</td><td>5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3'</td></tr><br />
<tr><td>VP16.Rev</td><td>Reverse primer for VP16.Hind.Fwd PCR - no modification</td><td>5'-CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3'</td></tr><br />
<tr><td>NLS.serine.BB.Fwd</td><td>Oligo to anneal NLS, with one bp difference to avoid self-complementarity </td><td>5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'</td></tr><br />
<tr><td>NLS.serine.BB.Rev</td><td>Complement to NLS.serine.BB.Fwd. for NLS annealing</td><td>5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3'</td></tr><br />
<tr><td>Fwd.BB.AtEXP2</td><td>for amplification/extraction of an arabidopsis germination-specific promoter</td><td>5'-CCT TTC TAG ACC TGG TGT GGT TTC TTT G-3'</td></tr><br />
<tr><td>Rev.BB.AtEXP2</td><td>for amplification/extraction of an arabidopsis germination-specific promoter</td><td>5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3'</td></tr><br />
<tr><td>Fwd.BB.AtARP2</td><td>for amplification/extraction of the arabidopsis ARP2 promoter</td><td>5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3'</td></tr><br />
<tr><td>Rev.BB.AtARP2</td><td>for amplification/extraction of the arabidopsis ARP2 promoter</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT CTT CTC CGA TTT CTA TAG AG-3'</td></tr><br />
<tr><td>barnase2.BB.fwd</td><td>addition of biobrick sites/ends to barnase gene</td><td>5'-CCT TTC TAG AAT GGT ACC GGT TAT CAA CAC G-3'</td></tr><br />
<tr><td>BB.Barnase.Rev</td><td>addition of biobrick sites/ends to barnase gene</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA TCT GAT TTT TGT AAA GGT CTG-3'</td></tr><br />
<tr><td>NLS.BB.Fwd</td><td>addition of biobrick sites/ends to the arabidopsis NLS</td><td>5'-/5Phos/CTA GAT CTT CTG TTG TTC ATC CAT AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'</td></tr><br />
<tr><td>NLS.BB.Rev</td><td>addition of biobrick sites/ends to the arabidopsis NLS</td><td>5'/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA-3'</td></tr><br />
<tr><td>LacIn.BB.Fwd</td><td>Addition of NLS to the Lac Inhibitor gene to produce LacIN</td><td>5'-CCT TGA ATT CGC GGC CGC ATC TAG AAT GAA AC AGT AAC GTT ATA CGA TGT C-3'</td></tr><br />
<tr><td>LacIN.BB.Rev</td><td>Addition of NLS to the Lac Inhibitor gene to produce LacIN</td><td>5' AAG GTC GCA GCA GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA CTG CCC GCT TTC CAG TCG GGA AA-3'</td></tr><br />
<tr><td>BB.Gal4DBD.Fwd</td><td>Adding biobrick sites to Gal4 DNA Binding Domain</td><td>5'-CTT TTC TAG AAT GAA GCT ACT GTC TTC TAT C-3'</td></tr><br />
<tr><td>BB.Gal4DBD.Rev</td><td>Adding biobrick sites to Gal4 DNA Binding Domain</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT CGA TAC AGT CAA CTG TCT TTG-3'</td></tr><br />
<tr><td>BB.Barstar.Fwd</td><td>addition of biobrick sites/ends to barstar gene</td><td>5'CCT TTC TAG AAT GAA AAA AGC AGT CAT TAA C-3'</td></tr><br />
<tr><td>BB.Barstar.Rev</td><td>addition of biobrick sites/ends to barstar gene</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA AGA AAG TATA GAT GGT GAT GTC-3'</td></tr><br />
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<h1>parts and primers</h1><br />
<h2>Parts</h2><br />
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<tr><td><b>Name</b></td><td><b>Biobrick Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>Barnase</td><td><center>-</center></td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f1">link</a></td></tr><br />
<tr><td>Barstar</td><td><center>-</center></td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f2">link</a></td></tr><br />
<tr><td>LacIN</td><td> BBa_K382035 </td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr><br />
<tr><td>RXRLc</td><td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr><br />
<tr><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr><br />
<tr><td>VP16</td><td> Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f6">link</a></td></tr><br />
<tr><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr><br />
<tr><td>Gal4 DNA binding domain</td><td> Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f8">link</a></td></tr><br />
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<h2>Promoters</h2><br />
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<tr><td><b>Name</b></td><td><b>Description</b></td><td>Active conditions</td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>pENT CUP</td><td> Constitutive Plant Promoter</td><td>Constitutive</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f10">link</a></td></tr><br />
<tr><td>5xGal UAS</td><td> 5 time repeat of Gal4 upstream activating sequence</td><td>On in presense of dimerized EcR-Gal and RXRLc-VP16</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f11">link</a></td></tr><br />
<tr><td>Act2lacO</td><td>Actin2 promoter with Lac Inhibitor responsive sites (Lac O sites</td><td>Constitutive, inhibited by LacIN</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f12">link</a></td></tr><br />
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<h2>Primers</h2><br />
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr><br />
<tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr><br />
<tr><td>Exp2MutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CCT ACA CAC GGA ACT CCT GGA AAT TAA GAC CG-3'</td></tr><br />
<tr><td>Exp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CGG TCT TAA TTT CCA GGA ACT CCG TGT GTA GG-3'</td></tr><br />
<tr><td>Bam.Gal4.Fwd</td><td>Attaching BamH1 sites to Gal4 in preparation for yeast vector ligation</td><td>5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'</td></tr><br />
<tr><td>HmRXR.Hind.Rev</td><td>Attaching HindIII sites to HmRXR in preparation for yeast vector ligation</td><td>5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'</td></tr><br />
<tr><td>LcRXR.Hind.Rev</td><td>Attaching HindIII sites to LcRXR in preparation for yeast vector ligation</td><td>5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3'</td></tr><br />
<tr><td>VP16.Hind.Fwd</td><td>Attaching HindIII sites to VP16 in preparation for yeast vector ligation</td><td>5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3'</td></tr><br />
<tr><td>VP16.Rev</td><td>Reverse primer for VP16.Hind.Fwd PCR - no modification</td><td>5'-CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3'</td></tr><br />
<tr><td>NLS.serine.BB.Fwd</td><td>Oligo to anneal NLS, with one bp difference to avoid self-complementarity </td><td>5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'</td></tr><br />
<tr><td>NLS.serine.BB.Rev</td><td>Complement to NLS.serine.BB.Fwd. for NLS annealing</td><td>5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3'</td></tr><br />
<tr><td>Fwd.BB.AtEXP2</td><td>for amplification/extraction of an arabidopsis germination-specific promoter</td><td>5'-CCT TTC TAG ACC TGG TGT GGT TTC TTT G-3'</td></tr><br />
<tr><td>Rev.BB.AtEXP2</td><td>for amplification/extraction of an arabidopsis germination-specific promoter</td><td>5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3'</td></tr><br />
<tr><td>Fwd.BB.AtARP2</td><td>for amplification/extraction of the arabidopsis ARP2 promoter</td><td>5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3'</td></tr><br />
<tr><td>Rev.BB.AtARP2</td><td>for amplification/extraction of the arabidopsis ARP2 promoter</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT CTT CTC CGA TTT CTA TAG AG-3'</td></tr><br />
<tr><td>barnase2.BB.fwd</td><td>addition of biobrick sites/ends to barnase gene</td><td>5'-CCT TTC TAG AAT GGT ACC GGT TAT CAA CAC G-3'</td></tr><br />
<tr><td>BB.Barnase.Rev</td><td>addition of biobrick sites/ends to barnase gene</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA TCT GAT TTT TGT AAA GGT CTG-3'</td></tr><br />
<tr><td>NLS.BB.Fwd</td><td>addition of biobrick sites/ends to the arabidopsis NLS</td><td>5'-/5Phos/CTA GAT CTT CTG TTG TTC ATC CAT AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'</td></tr><br />
<tr><td>NLS.BB.Rev</td><td>addition of biobrick sites/ends to the arabidopsis NLS</td><td>5'/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA-3'</td></tr><br />
<tr><td>LacIn.BB.Fwd</td><td>Addition of NLS to the Lac Inhibitor gene to produce LacIN</td><td>5'-CCT TGA ATT CGC GGC CGC ATC TAG AAT GAA AC AGT AAC GTT ATA CGA TGT C-3'</td></tr><br />
<tr><td>LacIN.BB.Rev</td><td>Addition of NLS to the Lac Inhibitor gene to produce LacIN</td><td>5' AAG GTC GCA GCA GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA CTG CCC GCT TTC CAG TCG GGA AA-3'</td></tr><br />
<tr><td>BB.Gal4DBD.Fwd</td><td>Adding biobrick sites to Gal4 DNA Binding Domain</td><td>5'-CTT TTC TAG AAT GAA GCT ACT GTC TTC TAT C-3'</td></tr><br />
<tr><td>BB.Gal4DBD.Rev</td><td>Adding biobrick sites to Gal4 DNA Binding Domain</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT CGA TAC AGT CAA CTG TCT TTG-3'</td></tr><br />
<tr><td>BB.Barstar.Fwd</td><td>addition of biobrick sites/ends to barstar gene</td><td>5'CCT TTC TAG AAT GAA AAA AGC AGT CAT TAA C-3'</td></tr><br />
<tr><td>BB.Barstar.Rev</td><td>addition of biobrick sites/ends to barstar gene</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA AGA AAG TATA GAT GGT GAT GTC-3'</td></tr><br />
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<h1>abstract</h1><br />
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As with any genetically modified organisms, keeping the iGarden plants safely contained is of the utmost importance. We developed a ‘fence’ system which ensures that all the plants stay inside the iGarden, preventing the spread of modified plants beyond where they are wanted.<br />
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The genetic fence operates by positive containment, meaning that the gardener chooses a fenced area in which the plants can grow, and outside of which they cannot. This ensures that any accidental spillage of iGarden seeds will not result in unwanted plant growth or spread of the modified genes.<br />
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Gardners will activate the fence by mixing a ‘fence compound’ into the water used for the garden during the first few weeks after planting. Once the plants have established themselves, the garden can be watered without the fence compound until another round of seeds are planted. The fence compound, a small molecule, is very safe for people, animals and the environment.<br />
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<h1>parts and primers</h1><br />
<h2>RNAi constructs</h2><br />
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<tr><td><b>Biobrick Name</b></td><td><b>Description</b></td><td><b>Size</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>BBa_K382010</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c1">link</a></td></tr><br />
<tr><td>BBa_K382011</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c2">link</a></td></tr><br />
<tr><td>BBa_K382012</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c3">link</a></td></tr><br />
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<h2>Primers</h2><br />
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>CP1</td><td>LUT2 I</td><td>gaTAACTTCCTAGTAACCCGCATtctctcttttgtattcc</td></tr><br />
<tr><td>CP2</td><td>LUT2 II</td><td>gaATGCGGGTTACTAGGAAGTTAtcaaagagaatcaatga</td></tr><br />
<tr><td>CP3</td><td>LUT2 III</td><td>gaATACGGGTTACTACGAAGTTTtcacaggtcgtgatatg</td></tr><br />
<tr><td>CP4</td><td>LUT2 IV</td><td>gaAAACTTCGTAGTAACCCGTATtctacatatatattcct</td></tr><br />
<tr><td>CP13</td><td>BETA-OHASE I</td><td>gaTCGTTTAAATCGAAGAGACGCtctctcttttgtattcc</td></tr><br />
<tr><td>CP14</td><td>BETA-OHASE II</td><td>gaGCGTCTCTTCGATTTAAACGAtcaaagagaatcaatga</td></tr><br />
<tr><td>CP15</td><td>BETA-OHASE III</td><td>gaGCATCTCTTCGATATAAACGTtcacaggtcgtgatatg</td></tr><br />
<tr><td>CP16</td><td>BETA-OHASE IV</td><td>gaACGTTTATATCGAAGAGATGCtctacatatatattcct</td></tr><br />
<tr><td>CP9</td><td>LYC I</td><td>gaTAATTATCCAACGACTTGCAGtctctcttttgtattcc</td></tr><br />
<tr><td>CP10</td><td>LYC II</td><td>gaCTGCAAGTCGTTGGATAATTAtcaaagagaatcaatga</td></tr><br />
<tr><td>CP11</td><td>LYC III</td><td>gaCTACAAGTCGTTGCATAATTTtcacaggtcgtgatatg</td></tr><br />
<tr><td>CP12</td><td>LYC IV</td><td>gaAAATTATGCAACGACTTGTAGtctacatatatattcct</td></tr><br />
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<h1>References</h1><br />
<ul><br />
<li>Chappell, Joseph. "Valencene synthase – a biochemical magician and harbinger of transgenic aromas." <i> Trends in Plant Science</i>. 1 June 2004.</li><br />
<li>Sharon-Asa et al. Citrus fruit flavor and aroma biosynthesis: isolation, functional characterization, and developmental regulation of Cstps1, a key gene in the production of the sesquiterpene aroma compound valencene. Plant J (2003) vol. 36 (5) pp. 664-74</li><br />
<li>Masferrer et al. Overexpression of Arabidopsis thaliana farnesyl diphosphate synthase (FPS1S) in transgenic Arabidopsis induces a cell death/senescence-like response and reduced cytokinin levels. Plant J (2002) vol. 30 (2) pp. 123-32</li><br />
<li>Manzano et al. Overexpression of farnesyl diphosphate synthase in Arabidopsis mitochondria triggers light-dependent lesion formation and alters cytokinin homeostasis. Plant Mol Biol (2006) vol. 61 (1-2) pp. 195-213</li><br />
<li>Coutu et al. pORE: a modular binary vector series suited for both monocot and dicot plant transformation. Transgenic Res (2007) vol. 16 (6) pp. 771-81</li><br />
<li>Chappell. Valencene synthase--a biochemical magician and harbinger of transgenic aromas. Trends Plant Sci (2004) vol. 9 (6) pp. 266-9</li><br />
<li>Chang et al. Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol (2007) vol. 3 (5) pp. 274-7</li><br />
<li>Masuda et al. Cloning and sequencing of a cDNA encoding a taste-modifying protein, miraculin. Gene (1995) vol. 161 (2) pp. 175-7</li><br />
<li>Assadi-Porter et al. Sweetness determinant sites of brazzein, a small, heat-stable, sweet-tasting protein. Arch Biochem Biophys (2000) vol. 376 (2) pp. 259-65</li><br />
<li>Walters et al. Design and evaluation of new analogs of the sweet protein brazzein. Chem Senses (2009) vol. 34 (8) pp. 679-83</li><br />
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<h1>confirmation results</h1><br />
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<p><br />
The two flavors that are currently ready for transformation into plants are the "taste-inverter" <a href="http://en.wikipedia.org/wiki/Miraculin">miraculin</a> and the sweetener <a href="http://en.wikipedia.org/wiki/Brazzein">brazzein</a>. Given the long time-frame of plant transformation we used two different assays in <i>E. Coli</i> to confirm that our proteins could indeed be transcribed and translated. The results of those assays are shown here.<br />
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<h2>confirmation with <i>YFP-2x</i> tags</h2><br />
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<p><br />
In order to confirm that miraculin and brazzein are able to be expressed in <i>E. Coli</i>, we attached a <i>YFP-2x</i> tag sequence to both the N- and C-termini of each protein. The proteins were placed under an IPTG-expressible promoter, and spectrophotometry was used to determine the level of YFP fluorescence against a baseline, untagged protein. Figure 1 shows relative-fluorescence at times post induction. In all circumstances the levels of YFP-fluorescence increased.<br />
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<div>Figure 1 &nbsp; <a href="https://static.igem.org/mediawiki/2010/7/77/YFP_Fig_1-crop.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/2010/7/77/YFP_Fig_1-crop.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/2010/7/77/YFP_Fig_1-crop.jpg" width="300px" border=0><br />
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<u>Figure 1. Induced expression of YFP-tagged Miraculin and Brazzein in <i>E. Coli</i></u></br><br />
Figure 1 (A) through (D) are normalized plots of miraculin and brazzein YFP-fused constructs expressed in E. Coli. <i>YFP-2x</i> tags were attached to either N- or C- terminus to ensure that folding was not hindered. In all cases relative YFP fluorescence had appreciably increased after 120 minutes as compared to the non-induced <i>E. Coli</i><br />
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<h2>confirmation by western blot</h2><br />
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<p><br />
A western blot assay was performed to check for <i>E. Coli</i> expression of miraculin and brazzein. Proteins tagged at either the N- or C- terminus were placed under the control of an IPTG-inducible promoter. In the miraculin assay, no protein expression was seen. It is possible that the protein does not express well in <i>E. Coli</i>, or that the plant-specific codon optimization of the proteins resulted in reduced expressibility. Brazzein, specifically C-terminus tagged brazzein was seen to be highly expressed in <i>E. Coli</i>.<br />
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<div>Figure 2 &nbsp; <a href="https://static.igem.org/mediawiki/2010/a/af/Western_Fig_2-crop.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/2010/a/af/Western_Fig_2-crop.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/2010/a/af/Western_Fig_2-crop.jpg" width="300px" border=0><br />
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<u>Figure 2. Western Blot of Miraculin and Brazzein Expression in <i>E. Coli</i></u></br><br />
Proteins were tagged using a standard <i>StrepII</i> tag, attached to either the N- or C- terminus. Miraculin (A) does not appear to have been expressed in high enough quantities to be visualized. The expected protein weight is 25 kDa. Brazzein (B) shows strong expression of a protein in the 10-15 kDa range. Brazzein has an expected weight of 6.5 kDa, a discrepancy that we have attributed to inconsistencies in the gel.<br />
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<h2>expression in <i>Arabidopsis</i></h2><br />
<p>We are still waiting for the plants to grow to a size large enough that we can collect samples to verify expression, but we have selected for plants that have integrated the glufosinate resistance marker along with the miraculin and brazzein expression constructs.</p><br />
<br />
<div><strong>Miraculin:</strong> <a href="https://static.igem.org/mediawiki/2010/5/53/V21selection.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><br />
<a href="https://static.igem.org/mediawiki/2010/5/53/V21selection.jpg" id="single_image"><br />
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<div><strong>Brazzein:</strong> <a href="https://static.igem.org/mediawiki/2010/f/fb/V25selection.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><br />
<a href="https://static.igem.org/mediawiki/2010/f/fb/V25selection.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/2010/f/fb/V25selection.jpg" width="300px" border=0><br />
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<p><center><b>Stay tuned to our Wiki for updates!</b></center></p><br />
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<h1>parts and primers</h1><br />
<h2>Primers</h2><br />
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>J45004_F</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45004_F">link</a></td></tr><br />
<tr><td>J45004_R</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45004_R">link</a></td></tr><br />
<tr><td>J45017_F</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45017_F">link</a></td></tr><br />
<tr><td>J45017_R</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45017_R">link</a></td></tr><br />
<tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/NOSterm_BB_F">link</a></td></tr><br />
<tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/NOSterm_BB_R">link</a></td></tr><br />
<tr><td>Stop_NOSterm_BB_F</td><td>NOSt plant specific terminator with stop codon on end</td><td><a href="https://2010.igem.org/Team:Harvard/Stop_NOSterm_BB_F">link</a></td></tr><br />
<tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/pENTCUP2_BB_F">link</a></td></tr><br />
<tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/pENTCUP2_BB_R">link</a></td></tr><br />
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<h2>Parts</h2><br />
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Length</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>BBa_K382020</td><td><i>Arabidopsis</i> optimized Brazzein</td><td>195</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<tr><td>BBa_K382021</td><td><i>Arabidopsis</i> optimized Miraculin</td><td>660</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<tr><td>BBa_K382022</td><td>pENTCUP2 plant specific promoter</td><td>503</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<tr><td>BBa_K382023</td><td>NosT plant specific terminator</td><td>253</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<tr><td>BBa_K382024</td><td>NosT plant specific terminator with STOP codon</td><td>256</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<tr><td>BBa_K382025</td><td><i>Arabidopsis</i> optimized Miraculin with StrepII N-terminus tag</td><td>696</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<tr><td>BBa_K382026</td><td><i>Arabidopsis</i> optimized Brazzein with StrepII N-terminus tag</td><td>201</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<tr><td>BBa_K382027</td><td><i>Arabidopsis</i> optimized Miraculin with StrepII C-terminus tag</td><td>696</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<tr><td>BBa_K382028</td><td><i>Arabidopsis</i> optimized Brazzein with StrepII C-terminus tag</td><td>201</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
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</html></div>Pmboylehttp://2010.igem.org/Team:Harvard/flavor/partsTeam:Harvard/flavor/parts2010-10-27T00:13:02Z<p>Pmboyle: </p>
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<h1>parts and primers</h1><br />
<h2>Primers</h2><br />
<table cellspacing="0"><br />
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>J45004_F</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45004_F">link</a></td></tr><br />
<tr><td>J45004_R</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45004_R">link</a></td></tr><br />
<tr><td>J45017_F</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45017_F">link</a></td></tr><br />
<tr><td>J45017_R</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45017_R">link</a></td></tr><br />
<tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/NOSterm_BB_F">link</a></td></tr><br />
<tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/NOSterm_BB_R">link</a></td></tr><br />
<tr><td>Stop_NOSterm_BB_F</td><td>NOSt plant specific terminator with stop codon on end</td><td><a href="https://2010.igem.org/Team:Harvard/Stop_NOSterm_BB_F">link</a></td></tr><br />
<tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/pENTCUP2_BB_F">link</a></td></tr><br />
<tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/pENTCUP2_BB_R">link</a></td></tr><br />
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<tr><td><b>Biobrick Name</b></td><td><b>Description</b></td><td><b>Length</b></td><td><b>Sequence</b></td></tr><br />
<tr><td>BBa_K382020</td><td>Arabidopsis optimized Brazzein</td><td>196</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr><br />
<tr><td>BBa_K382021</td><td>Arabidopsis optimized Miraculin</td><td>660</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr><br />
<tr><td>BBa_K382022</td><td>pENTCUP2 plant specific promoter</td><td>503</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr><br />
<tr><td>BBa_K382023</td><td>NosT plant specific terminator</td><td>253</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr><br />
<tr><td>BBa_K382024</td><td>NosT plant specific terminator with STOP codon</td><td>256</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr><br />
<tr><td>BBa_K382025</td><td>Arabidopsis optimized Miraculin with StrepII N-terminus tag</td><td>696</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr><br />
<tr><td>BBa_K382026</td><td>Arabidopsis optimized Brazzein with StrepII N-terminus tag</td><td>201</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr><br />
<tr><td>BBa_K382027</td><td>Arabidopsis optimized Miraculin with StrepII C-terminus tag</td><td>696</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr><br />
<tr><td>BBa_K382028</td><td>Arabidopsis optimized Brazzein with StrepII C-terminus tag</td><td>201</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr><br />
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</html></div>Pmboylehttp://2010.igem.org/Team:Harvard/flavor/flavorsTeam:Harvard/flavor/flavors2010-10-27T00:11:23Z<p>Pmboyle: </p>
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<h1>flavors</h1><br />
<p>Flavors are perhaps to most interactive parts of the iGarden project. We currently have 2 flavors inserted into plants that are currently growing, with options and ideas for an additional three.</p><br />
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<br><br />
<h2>miraculin</h2><br />
<br />
<p> Miraculin is a 'flavor inverting' protein, found naturally in the fruit of the plant <i>Synsepalum dulcificum</i>. Not sweet by itself, miraculin binds to taste receptors on the tongue, possibly altering the structure of the receptors and causing traditionally 'sour' flavors to be received as 'sweet'. </p><br />
<br />
<p> We have obtained, synthesized and BioBricked the DNA sequence of miraculin. This has allowed us to work with it in the usual BioBrick fashion, facilitating insertion into the agrobacterium expression vector.</p> <br />
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<p> Preliminary <a href="https://2010.igem.org/Team:Harvard/flavor/results">tests</a> in <i>E. Coli</i> have been positive, with our YFP-tagged proteins showing definite expression </p><br />
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<h2>brazzein</h2><br />
<br />
<p> Brazzein is a sweet-tasting protein that is found in the Western African fruit, the Oubli. The protein consists of a 54 amino acid sequence. It is sweeter than sugar and is used as an alternative low calorie sweetener. </p><br />
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<p> We have obtained, synthesized and BioBricked the DNA sequence of brazzein. We were then able to flank this sequence with a plant specific promoter and terminator and insert this construct into the agrobacterium expression vector. </p><br />
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<p> Preliminary tests in <i>E. Coli</i> have been positive, with our YFP-tagged proteins showing definite expression and a Western Blot also showing successful expression of brazzein. </p><br />
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<h2>valencene</h2><br />
<br />
<p> We originally tried to extract RNA from Valencia oranges and then make a cDNA library. We then used PCR to amplify the Valencene sequence. Valencene is a gene that codes for a molecule that gives Valencia oranges their citrus flavor and aroma. </p><br />
<br />
<p> Unfortunately, after three attempts, we were not able to obtain a viable cDNA library. We then tried to extract genomic DNA. However, none of our PCR tries worked from the genomic DNA. We therefore plan to synthesize the valencene synthase gene based on available sequences.</p><br />
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<h2>wintergreen</h2><br />
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<div>wintergreen expression system &nbsp; <a href="https://static.igem.org/mediawiki/2010/b/bd/Wintergreen_pathway.png" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/2010/b/bd/Wintergreen_pathway.png" id="single_image"><br />
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<p style="padding:10px">The MIT 2006 iGEM team created a BioBricked system to express <i>methyl salicyate</i>, a mint smelling compound. By swapping-in plant specific promoters and inserting the system into the pORE open vector, we would be able to create mint-smelling plants. </p></td><br />
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<h2>banana</h2><br />
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<div>banana expression system &nbsp; <a href="https://static.igem.org/mediawiki/2010/a/ab/BBa_J45200.png" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/2010/a/ab/BBa_J45200.png" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/2010/a/ab/BBa_J45200.png" width="300px" border=0><br />
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The Banana Scent pathway is also from the MIT 2006 iGEM team and is another example of how the iGarden system is well suited for the modular nature of the Parts Registry. Through addition of plant-specific promoters, and use of the now-BioBrick compatible pORE vectors, banana smelling plants could be achieved.<br />
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