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http://2010.igem.org/Team:Chiba/Safety
Team:Chiba/Safety
2010-11-19T07:59:46Z
<p>Lily.ch: </p>
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<td width="800px"><font size="5" face="verdana">Safety issue</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<strong><font size="2">1. Would any of your project ideas raise safety issues?<br />
</strong></font><br><br />
- Not at all. All our circuits are made of genetic regulators and reporters (fluorescent proteins).<br><br />
Our project goal -double click system- is designed to improve the safety in operating modified organisms.<br><br />
<br />
<br><br />
<strong><font size="2">2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?<br />
</strong></font><br><br />
- Our biobricks are composed of gene regulators, RNA polymerase, hybrid promoters, and fluorescent proteins only. None of them (and the combination of those parts/ devices) could raise the safety issue.<br />
Thus, we are working on the super-safe side of Synthetic Biology!<br><br />
<br />
<br><br />
<strong><font size="2">3.Is there a local biosafety group, committee, or review board at your institution?</strong></font><br><br />
- Yes. Our institution has the committee to deal with all the safety issues, which occur in bioengineering and we have got the permission of our project.<br><br />
<br />
<br />
<br><br />
<strong><font size="2">4.Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and -systems be made even safer through biosafety engineering?</strong></font><br><br />
<br />
- Definitely, safety issues in iGEM is very important and false input should be eliminated, so safty system like our project would quite useful. <br><br />
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[[Safety |Go to safety page]].</div>
Lily.ch
http://2010.igem.org/File:GFP.png
File:GFP.png
2010-10-28T04:00:01Z
<p>Lily.ch: uploaded a new version of "Image:GFP.png"</p>
<hr />
<div>Differential equation for GFP</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-28T03:27:03Z
<p>Lily.ch: /* GFP Pulse Generator Result */</p>
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__NOTOC__<br />
<br><br><br />
[[Team:Chiba/System 1|<<Back]]<br />
<br><br><br />
<font size=5>Version 1 :Evaluating Core Devices</font><br><br />
<br />
==<font size="4">Successful operation of this system requires</font>==<br />
===1. Generator of T7 RNA Polymerase pulse===<br />
In response to both the 1st and the 2nd input, T7 RNAP has to be effective for a moment then get silent. To fulfill this task,<br />
*Lux/CI434 hybrid promoter:<br />
**must be activated by LuxR in the presence of AHLs<br><br />
**must be strictly repressed by CI434<br><br />
**must NOT be repressed by CI (another repressor components in the circuit)<br><br />
*CI434<br />
**should be expressed upon AHL input<br><br />
**must become effective after a certain time (delayed repression)<br><br />
**should tightly repress <br><br />
*T7RNAP<br />
**must be cleared off as soon as possible when its expression is stopped <br><br />
<br />
===2. Output module (AND gates of T7RNAP & (NOT CI)) ===<br />
*T7/CI promoter should be activated by T7 RNAP<br />
*T7/CI promoter should be tightly repressed by CI<br />
*CI should be degraded off after expression shutted off(upon AHL input)<br><br />
<br />
===3. Tuning the timing.===<br />
*During the T7 RNAP pulse, CI should keep its repression power. ( T7RNAP pulse should be gone before repression by CI start decreasing)<br />
*'Invertimer' should create enough time to clear off the repression by CI<br><br />
<br />
==<font size="4"> GFP Pulse Generator Result</font>==<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396006 BBa_K396006]<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]<br />
[[Image:名称未設定.png]]<br />
<br />
===Protocol===<br />
E. coli strain DH10B was used for the pulse-generator experiments.<br />
Co-transformed cell were pre-cultured in test tubes for overnight at 37C, 200rpm.<br />
Dilute the culture 1:100 into 2ml of fresh medium and grow for an additional 5 hours in Supplemented M9 medium .<br />
For the liquid experiments, expression was induced at the log phase (OD600 of 0.4) <br />
by the addition of AHL (3OC6HSL)at the appropriate concentration. <br />
One-milliliter samples were taken every 5 min.<br />
<br />
<br />
===Result===<br />
By now, there are no obvious pulse observed.The construction we use for measuring GFP is lux/cI434 hybrid promoter following by destabilized GFP. We also check GFP with the same <br />
degradation tag following by hybrid promoter as a positive control, it neither generate any fluorescent.Maybe because the GFP expression is too low to be investigate because we use <br />
weak RBS and GFP with LVA.We will continue with investigating GFP pulse. Except that, we <br />
want to western blot T7 RNA Polymerase pulse and output GFP in plasmid 1 whose transcription<br />
factors are T7 RNA Polymerase and cI protein.We hope there will be GFP pulse generated when co-transform plasmid 1 and 2 because of T7 RNA Polymerase amplifier.</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-28T03:26:32Z
<p>Lily.ch: /* GFP Pulse Generator Result */</p>
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__NOTOC__<br />
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[[Team:Chiba/System 1|<<Back]]<br />
<br><br><br />
<font size=5>Version 1 :Evaluating Core Devices</font><br><br />
<br />
==<font size="4">Successful operation of this system requires</font>==<br />
===1. Generator of T7 RNA Polymerase pulse===<br />
In response to both the 1st and the 2nd input, T7 RNAP has to be effective for a moment then get silent. To fulfill this task,<br />
*Lux/CI434 hybrid promoter:<br />
**must be activated by LuxR in the presence of AHLs<br><br />
**must be strictly repressed by CI434<br><br />
**must NOT be repressed by CI (another repressor components in the circuit)<br><br />
*CI434<br />
**should be expressed upon AHL input<br><br />
**must become effective after a certain time (delayed repression)<br><br />
**should tightly repress <br><br />
*T7RNAP<br />
**must be cleared off as soon as possible when its expression is stopped <br><br />
<br />
===2. Output module (AND gates of T7RNAP & (NOT CI)) ===<br />
*T7/CI promoter should be activated by T7 RNAP<br />
*T7/CI promoter should be tightly repressed by CI<br />
*CI should be degraded off after expression shutted off(upon AHL input)<br><br />
<br />
===3. Tuning the timing.===<br />
*During the T7 RNAP pulse, CI should keep its repression power. ( T7RNAP pulse should be gone before repression by CI start decreasing)<br />
*'Invertimer' should create enough time to clear off the repression by CI<br><br />
<br />
==<font size="4"> GFP Pulse Generator Result</font>==<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396006 BBa_K396006]<br />
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]<br />
[[Image:名称未設定.png]]<br />
<br />
===Protocol===<br />
E. coli strain DH10B was used for the pulse-generator experiments.<br />
Co-transformed cell were pre-cultured in test tubes for overnight at 37C, 200rpm.<br />
Dilute the culture 1:100 into 2ml of fresh medium and grow for an additional 5 hours in Supplemented M9 medium .<br />
For the liquid experiments, expression was induced at the log phase (OD600 of 0.4) <br />
by the addition of AHL (3OC6HSL)at the appropriate concentration. <br />
One-milliliter samples were taken every 5 min.<br />
<br />
<br />
===Result===<br />
By now, there are no obvious pulse observed.The construction we use for measuring GFP is lux/cI434 hybrid promoter following by destabilized GFP. We also check GFP with the same <br />
degradation tag following by hybrid promoter as a positive control, it neither generate any fluorescent.Maybe because the GFP expression is too low to be investigate because we use <br />
weak RBS and GFP with LVA.We will continue with investigating GFP pulse. Except that, we <br />
want to western blot T7 RNA Polymerase pulse and output GFP in plasmid 1 whose transcription<br />
factors are T7 RNA Polymerase and cI protein.We hope there will be GFP pulse generated when co-transform plasmid 1 and 2 because of T7 RNA Polymerase amplifier.</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-28T03:24:23Z
<p>Lily.ch: /* GFP Pulse Generator Result */</p>
<hr />
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<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
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[[Team:Chiba/System 1|<<Back]]<br />
<br><br><br />
<font size=5>Version 1 :Evaluating Core Devices</font><br />
===Successful operation of this system requires===<br />
====1. Generator of T7 RNA Polymerase pulse====<br />
In response to both the 1st and the 2nd input, T7 RNAP has to be effective for a moment then get silent. To fulfill this task,<br />
*Lux/CI434 hybrid promoter:<br />
**must be activated by LuxR in the presence of AHLs<br><br />
**must be strictly repressed by CI434<br><br />
**must NOT be repressed by CI (another repressor components in the circuit)<br><br />
*CI434<br />
**should be expressed upon AHL input<br><br />
**must become effective after a certain time (delayed repression)<br><br />
**should tightly repress <br><br />
*T7RNAP<br />
**must be cleared off as soon as possible when its expression is stopped <br><br />
<br />
====2. Output module (AND gates of T7RNAP & (NOT CI)) ====<br />
*T7/CI promoter should be activated by T7 RNAP<br />
*T7/CI promoter should be tightly repressed by CI<br />
*CI should be degraded off after expression shutted off(upon AHL input)<br><br />
<br />
====3. Tuning the timing.====<br />
*During the T7 RNAP pulse, CI should keep its repression power. ( T7RNAP pulse should be gone before repression by CI start decreasing)<br />
*'Invertimer' should create enough time to clear off the repression by CI<br><br />
<br />
===<font size="5"> GFP Pulse Generator Result</font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396006 BBa_K396006]<br />
[[Image:名称未設定.png]]<br />
<br />
<font size="4">Protocol</font><br />
------<br />
<br />
<font size="3"><br />
E. coli strain DH10B was used for the pulse-generator experiments.<br />
Co-transformed cell were pre-cultured in test tubes for overnight at 37C, 200rpm.<br />
Dilute the culture 1:100 into 2ml of fresh medium and grow for an additional 5 hours in Supplemented M9 medium .<br />
For the liquid experiments, expression was induced at the log phase (OD600 of 0.4) <br />
by the addition of AHL (3OC6HSL)at the appropriate concentration. <br />
One-milliliter samples were taken every 5 min.<br />
</font><br />
<br />
<br />
<font size="4">Result</font><br />
------<br />
<font size="3"><br />
By now, there are no obvious pulse observed.The construction we use for measuring GFP is lux/cI434 hybrid promoter following by destabilized GFP. We also check GFP with the same <br />
degradation tag following by hybrid promoter as a positive control, it neither generate any fluorescent.Maybe because the GFP expression is too low to be investigate because we use <br />
weak RBS and GFP with LVA.We will continue with investigating GFP pulse. Except that, we <br />
want to western blot T7 RNA Polymerase pulse and output GFP in plasmid 1 whose transcription<br />
factors are T7 RNA Polymerase and cI protein.We hope there will be GFP pulse generated when co-transform plasmid 1 and 2 because of T7 RNA Polymerase amplifier.<br />
</font></div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-28T03:11:35Z
<p>Lily.ch: /* 1-2. Pulse generator */</p>
<hr />
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<font size=6>Version 1 :</font><br />
===<font size="5">Successful operation of this system requires</font>===<br />
<font size="3"><br />
1. Generator of T7 RNA Polymerase pulse<br><br />
In response to both the 1st and the 2nd input, T7 RNAP has to be effective for a moment then<br> <br />
get silent. To fulfill this task,<br />
- Lux/CI434 hybrid promoter:<br />
-must be activated by LuxR in the presence of AHLs<br><br />
-must be strictly repressed by CI434<br><br />
-must NOT be repressed by CI (another repressor components in the circuit)<br><br />
- CI434<br />
-should be expressed upon AHL input<br><br />
-must become effective after a certain time (delayed repression)<br><br />
-should tightly repress <br><br />
- T7RNAP<br />
- must be cleared off as soon as possible when its expression is stopped <br><br />
<br />
<br />
2. Output module (AND gates of T7RNAP & (NOT CI)) <br />
- T7/CI promoter should be activated by T7 RNAP<br />
- T7/CI promoter should be tightly repressed by CI<br />
- CI should be degraded off after expression shutted off(upon AHL input)<br />
<br />
3. Tuning the timing.<br />
- During the T7 RNAP pulse, CI should keep its repression power.<br />
( T7RNAP pulse should be gone before repression by CI start decreasing)<br />
- 'Invertimer' should create enough time to clear off the repression by CI<br />
<br />
===<font size="5"> GFP Pulse Generator Result</font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396006 BBa_K396006]<br />
[[Image:名称未設定.png]]<br />
<br />
<font size="3"><br />
Protocol<br />
------<br />
E. coli strain DH10B was used for the pulse-generator experiments.<br />
Co-transformed cell were pre-cultured in test tubes for overnight at 37C, 200rpm.<br />
Dilute the culture 1:100 into 2ml of fresh medium and grow for an additional 5 hours in Supplemented M9 medium .<br />
For the liquid experiments, expression was induced at the log phase (OD600 of 0.4) <br />
by the addition of AHL (3OC6HSL)at the appropriate concentration. <br />
One-milliliter samples were taken every 5 min.<br />
<br />
<br />
Result<br />
------<br />
There are no obvious pulse observed.We use destabilized GFP at the downstream of pLux/cI434 <br />
hybrid promoter as a positive control, but it also didn't generate any fluorescent.Maybe because<br />
we use weak RBS and GFP with LVA, the GFP protein degradation is too soon to be observed. <br />
Our project focus on T7 RNA Polymerase pulse and the output GFP will be amplified. So we <br />
hope there will be GFP pulse generated when co-transform plasmid 1 and 2.</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-28T03:08:56Z
<p>Lily.ch: /* Requirement of realizing genetic double click system */</p>
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<font size=6>Version 1 :</font><br />
===<font size="5">Successful operation of this system requires</font>===<br />
<font size="3"><br />
1. Generator of T7 RNA Polymerase pulse<br><br />
In response to both the 1st and the 2nd input, T7 RNAP has to be effective for a moment then<br> <br />
get silent. To fulfill this task,<br />
- Lux/CI434 hybrid promoter:<br />
-must be activated by LuxR in the presence of AHLs<br><br />
-must be strictly repressed by CI434<br><br />
-must NOT be repressed by CI (another repressor components in the circuit)<br><br />
- CI434<br />
-should be expressed upon AHL input<br><br />
-must become effective after a certain time (delayed repression)<br><br />
-should tightly repress <br><br />
- T7RNAP<br />
- must be cleared off as soon as possible when its expression is stopped <br><br />
<br />
<br />
2. Output module (AND gates of T7RNAP & (NOT CI)) <br />
- T7/CI promoter should be activated by T7 RNAP<br />
- T7/CI promoter should be tightly repressed by CI<br />
- CI should be degraded off after expression shutted off(upon AHL input)<br />
<br />
3. Tuning the timing.<br />
- During the T7 RNAP pulse, CI should keep its repression power.<br />
( T7RNAP pulse should be gone before repression by CI start decreasing)<br />
- 'Invertimer' should create enough time to clear off the repression by CI<br />
<br />
===<font size="4">1-2. Pulse generator</font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396006 BBa_K396006]<br />
[[Image:名称未設定.png]]<br />
<br />
<font size="3"><br />
Protocol<br />
------<br />
E. coli strain DH10B was used for the pulse-generator experiments.<br />
Co-transformed cell were pre-cultured in test tubes for overnight at 37C, 200rpm.<br />
Dilute the culture 1:100 into 2ml of fresh medium and grow for an additional 5 hours in Supplemented M9 medium .<br />
For the liquid experiments, expression was induced at the log phase (OD600 of 0.4) <br />
by the addition of AHL (3OC6HSL)at the appropriate concentration. <br />
One-milliliter samples were taken every 5 min.<br />
<br />
<br />
Result<br />
------<br />
There are no obvious pulse observed.We use destabilized GFP at the downstream of pLux/cI434 <br />
hybrid promoter as a positive control, but it also didn't generate any fluorescent.Maybe because<br />
we use weak RBS and GFP with LVA, the GFP protein degradation is too soon to be observed. <br />
Our project focus on T7 RNA Polymerase pulse and the output GFP will be amplified. So we <br />
hope there will be GFP pulse generated when co-transform plasmid 1 and 2.</div>
Lily.ch
http://2010.igem.org/Team:Chiba/Project
Team:Chiba/Project
2010-10-28T02:56:38Z
<p>Lily.ch: /* Double Click Bacteria */</p>
<hr />
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===<font size="5">Double Click Bacteria</font>===<br />
-----<br />
<font size="3">We’re inspired by double-click of computer’s mouse. It doesn’t react to the first click but does react when it is accompanied by the second one. This is one of the most accepted, familiarized, and proven mechanism to diminish the erroneous operation. This fail-safe technology should find various uses also in biotechnology. <br />
Here is what we envision: once in a while, bacteria might receive input which wasn’t intended. But they are careful enough (they know Homo sapiens always make mistake!) so that they ignore the input for the first time. However, when they receive the two inputs in succession, they finally judge the input(s) are real, and start taking the action as programmed. Also, they are smart enough to distinguish double-clicking from the two separate (or erroneous) inputs. <br />
First, the circuit must distinguish the second input (stimulus) from the first one, even though they are chemically physically the same . To realize this, we need the counting device. Second, the circuit must distinguishes W-click from two separate clicks; the circuit reacts only when the first and the second input are given in short period of time. Thus, the genetic counter should be coupled with the genetic timers. After a certain time after the first input (timeout), the circuit gets back to the initial state so that the next stimulous is recognized as the first input.<br />
<html><br />
<center><br />
<table width="80%"><tr><td><br />
<img src="https://static.igem.org/mediawiki/2010/7/7c/Click.jpg"></td><td><br />
<object width="480" height="385"><param name="movie" value="http://www.youtube.com/v/vQbNnGbuXj0?fs=1&amp;hl=ko_KR"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/vQbNnGbuXj0?fs=1&amp;hl=ko_KR" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="480" height="385"></embed></object><br />
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<br><br><br><br />
<br />
===<font size="5">The Bottom Line is...</font>===<br />
#If there is only an input, nothing happens.<br />
#Duration of the input is not the matter. The circuit cares only the number of input.<br />
#However, a certain time after the 1st input, it returns to the initial state.<br />
#Giving two inputs in the limited time the circuit get activated (gives output).<br />
<Br><br><br />
<br />
===<font size="5">Systems</font>===<br />
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<a href="https://2010.igem.org/Team:Chiba/System_1"><img src="https://static.igem.org/mediawiki/2010/d/db/Chiba_plan2_1.jpg" border="0" width="300px" /><br>version 1</a><br />
</td><td style="text-align:center;"><br />
<a href="https://2010.igem.org/Team:Chiba/System_2"><img src="https://static.igem.org/mediawiki/2010/1/1a/Chiba_Sys2.jpg" border="0" width="300px" /><br>version 2</a><br />
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Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-28T02:51:56Z
<p>Lily.ch: /* Requirement of realizing genetic double click system */</p>
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<font size=6>Version 1 :</font><br />
===<font size="5">Requirement of realizing genetic double click system</font>===<br />
-----<br />
1. T7 RNA Polymerase pulse in response to 1st and 2nd input<br><br />
In response to 1st and 2nd input, T7 RNA polymerase generates as a pulse.<br><br />
To accomplish this, Lux/CI434 hybrid promoter should be activated or repressed stringently.<br><br />
Lux/cI434 hybrid promoter play an important role in our system.<br><br />
<br><br />
2. CI repression<br><br />
CI needs to work as a repressor of T7/CI-OR1 hybrid promoter for the 1st input and needs to<br />
degradate before 2nd input doesn't work as a repressor at 2nd input.<br />
To accomplish this, T7/CI-OR1 hybrid promoter should be activated or repressed stringently.<br><br />
We have confirmed the hybrid promoter work just as we want.<br><br />
<br><br />
3. Setting the fixed time<br><br />
There is the fixed time between 1st and 2nd input.<br />
To accomplish this, CI has to re-express after washout. (Data not shown)<br />
<br />
===<font size="4">1-2. Pulse generator</font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396006 BBa_K396006]<br />
[[Image:名称未設定.png]]<br />
<br />
<font size="3"><br />
Protocol<br />
------<br />
E. coli strain DH10B was used for the pulse-generator experiments.<br />
Co-transformed cell were pre-cultured in test tubes for overnight at 37C, 200rpm.<br />
Dilute the culture 1:100 into 2ml of fresh medium and grow for an additional 5 hours in Supplemented M9 medium .<br />
For the liquid experiments, expression was induced at the log phase (OD600 of 0.4) <br />
by the addition of AHL (3OC6HSL)at the appropriate concentration. <br />
One-milliliter samples were taken every 5 min.<br />
<br />
<br />
Result<br />
------<br />
There are no obvious pulse observed.We use destabilized GFP at the downstream of pLux/cI434 <br />
hybrid promoter as a positive control, but it also didn't generate any fluorescent.Maybe because<br />
we use weak RBS and GFP with LVA, the GFP protein degradation is too soon to be observed. <br />
Our project focus on T7 RNA Polymerase pulse and the output GFP will be amplified. So we <br />
hope there will be GFP pulse generated when co-transform plasmid 1 and 2.</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-28T02:39:33Z
<p>Lily.ch: /* Circuit Construction */</p>
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Double Click System</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>Version 2</span></a></li> </ul><br />
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<br />
=Version 1=<br />
==Overview==<br />
[[Image:Chiba_ani3.gif|right]]“Genetic double click system” - version 1 is based on the AND-gate which can be switched only when the same inputs are given twice in quick succession. This system requires the operations which never happen naturally to turn on the switch. So, we believe that this is a kind of safety device. System requires first input to be transient in order to allow second input. Unlike chemical inputs, light inputs are more suitable for this system because it does not remain after inputting. But, there is no light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. Next, this system is required to memorize the transient first input since this system is based on AND gate. In addition, this system requires fixed time between the first and second input like a computer mouse to recognize double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily.<br />
In our first circuit design, we compose a pulse-generator to memorize the input temporarily. Further more, the system is designed based on combination of a Fast-pulse and a Slow-pulse. [[Team:Chiba/System 1/Design|detail...]]<br />
<br />
==Circuit Construction==<br />
[[Image:Chiba_plan2_1.jpg|right|500px]]The circuit consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we use AHL as input and GFP output. So when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor. [[Team:Chiba/System 1/Design|detail...]]<br />
<br clear="all"><br />
<br />
==Results==<br />
===[[Team:Chiba/System_1/Construction_Process|Plasmid Construction]]===<br />
===[[Team:Chiba/System_1/Testing_components|Testing individual parts]]===<br />
===[[Team:Chiba/System_1/Result|Evaluating Core Devices]]===<br />
===Whole System===<br />
<br />
==[[Team:Chiba/System_1/Evaluation_subsystem|Summary for now...]]==</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-28T02:37:25Z
<p>Lily.ch: /* Overview */</p>
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<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li><br />
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<br />
<br />
=Version 1=<br />
==Overview==<br />
[[Image:Chiba_ani3.gif|right]]“Genetic double click system” - version 1 is based on the AND-gate which can be switched only when the same inputs are given twice in quick succession. This system requires the operations which never happen naturally to turn on the switch. So, we believe that this is a kind of safety device. System requires first input to be transient in order to allow second input. Unlike chemical inputs, light inputs are more suitable for this system because it does not remain after inputting. But, there is no light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. Next, this system is required to memorize the transient first input since this system is based on AND gate. In addition, this system requires fixed time between the first and second input like a computer mouse to recognize double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily.<br />
In our first circuit design, we compose a pulse-generator to memorize the input temporarily. Further more, the system is designed based on combination of a Fast-pulse and a Slow-pulse. [[Team:Chiba/System 1/Design|detail...]]<br />
<br />
==Circuit Construction==<br />
[[Image:Chiba_plan2_1.jpg|right|500px]]This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor. [[Team:Chiba/System 1/Design|detail...]]<br />
<br clear="all"><br />
<br />
==Results==<br />
===[[Team:Chiba/System_1/Construction_Process|Plasmid Construction]]===<br />
===[[Team:Chiba/System_1/Testing_components|Testing individual parts]]===<br />
===[[Team:Chiba/System_1/Result|Evaluating Core Devices]]===<br />
===Whole System===<br />
<br />
==[[Team:Chiba/System_1/Evaluation_subsystem|Summary for now...]]==</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-28T02:25:55Z
<p>Lily.ch: /* Lux/CI434 hybrid promoter */</p>
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<font size=6>Version 1 :</font><br />
==<font size="5">Testing individual parts</font>==<br />
-----<br />
To realize the genetic double click system, we made two plasmids. First one is ''Pulse generator'', which generate the pulse of T7 RNAP in response to the 1st and 2nd input. The second one is ''pulse -> GFP generator'', which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. <br />
The subparts of those plasmids are shown below.<br><br />
<center><br />
[[Image:Chiba_icon_1.jpg]]<br><br />
[[Image:Chiba_icon_2.jpg]]<br><br />
</center><br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
==1. Pulse generator==<br />
For Pulse generator, the parts shown below were checked.Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
===T7/CI-OR1 hybrid promoter===<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000 BBa_K396000]<br />
T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.<br />
====Experiment&Results====<br />
[[Image:CI-1.jpg|frame|center|Fig. How to check the parts]]<br />
[[Image:CI-2.jpg|frame|center|Fig. Data: Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation.]]<br />
*Dubendorf, J.W. & Studier, F.W. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. Journal of Molecular Biology 219, 45-59 (1991).<br />
<br />
<br />
==2. Pulse to GFP generator==<br />
For GFP generator, the parts shown below were checked.<br />
<br />
===Lux/CI434 hybrid promoter===<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
====Rationale====<br />
[[image:Hybrid.png]]<br />
<br><br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) at downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation.<br />
====Ref====<br />
#http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
#http://partsregistry.org/Part:BBa_I12040<br />
#Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101: 6355-6360.</div>
Lily.ch
http://2010.igem.org/File:Hybrid.png
File:Hybrid.png
2010-10-28T02:24:49Z
<p>Lily.ch: </p>
<hr />
<div></div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-28T02:15:36Z
<p>Lily.ch: /* 1-2. Pulse generator */</p>
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<font size=6>Version 1 :</font><br />
===<font size="5">Requirement of realizing genetic double click system</font>===<br />
-----<br />
1. T7 RNAP pulse in response to 1st and 2nd input<br><br />
In response to 1st and 2nd input, T7 RNAP has to express as pulse.<br><br />
To accomplish this, Lux/CI434 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part.<br><br />
<br><br />
2. CI repression<br><br />
CI needs to work as a repressor of T7/CI-OR1 hybrid promoter at 1st input and needs to<br />
have degradated before 2nd input not to work as a repressor at 2nd input.<br />
To accomplish this, T7/CI-OR1 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part).<br><br />
<br><br />
3. Setting the fixed time<br><br />
There is the fixed time between 1st and 2nd input.<br />
To accomplish this, CI has to re-express after washout. (Data not shown)<br />
<br />
<br />
===<font size="4">1-2. Pulse generator</font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396006 BBa_K396006]<br />
[[Image:名称未設定.png]]<br />
<br />
<font size="3"><br />
Protocol<br />
------<br />
E. coli strain DH10B was used for the pulse-generator experiments.<br />
Co-transformed cell were pre-cultured in test tubes for overnight at 37C, 200rpm.<br />
Dilute the culture 1:100 into 2ml of fresh medium and grow for an additional 5 hours in Supplemented M9 medium .<br />
For the liquid experiments, expression was induced at the log phase (OD600 of 0.4) <br />
by the addition of AHL (3OC6HSL)at the appropriate concentration. <br />
One-milliliter samples were taken every 5 min.<br />
<br />
<br />
Result<br />
------<br />
There are no obvious pulse observed.We use destabilized GFP at the downstream of pLux/cI434 <br />
hybrid promoter as a positive control, but it also didn't generate any fluorescent.Maybe because<br />
we use weak RBS and GFP with LVA, the GFP protein degradation is too soon to be observed. <br />
Our project focus on T7 RNA Polymerase pulse and the output GFP will be amplified. So we <br />
hope there will be GFP pulse generated when co-transform plasmid 1 and 2.</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-28T02:14:31Z
<p>Lily.ch: /* 1-2. Pulse generator */</p>
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<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li><br />
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<font size=6>Version 1 :</font><br />
===<font size="5">Requirement of realizing genetic double click system</font>===<br />
-----<br />
1. T7 RNAP pulse in response to 1st and 2nd input<br><br />
In response to 1st and 2nd input, T7 RNAP has to express as pulse.<br><br />
To accomplish this, Lux/CI434 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part.<br><br />
<br><br />
2. CI repression<br><br />
CI needs to work as a repressor of T7/CI-OR1 hybrid promoter at 1st input and needs to<br />
have degradated before 2nd input not to work as a repressor at 2nd input.<br />
To accomplish this, T7/CI-OR1 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part).<br><br />
<br><br />
3. Setting the fixed time<br><br />
There is the fixed time between 1st and 2nd input.<br />
To accomplish this, CI has to re-express after washout. (Data not shown)<br />
<br />
<br />
===<font size="4">1-2. Pulse generator</font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396006 BBa_K396006]<br />
[[Image:名称未設定.png]]<br />
<br />
Protocol<br />
------<br />
E. coli strain DH10B was used for the pulse-generator experiments.<br />
Co-transformed cell were pre-cultured in test tubes for overnight at 37C, 200rpm.<br />
Dilute the culture 1:100 into 2ml of fresh medium and grow for an additional 5 hours in Supplemented M9 medium .<br />
For the liquid experiments, expression was induced at the log phase (OD600 of 0.4) <br />
by the addition of AHL (3OC6HSL)at the appropriate concentration. <br />
One-milliliter samples were taken every 5 min.<br />
<br />
<br />
Result<br />
------<br />
There are no obvious pulse observed.We use destabilized GFP at the downstream of pLux/cI434 <br />
hybrid promoter as a positive control, but it also didn't generate any fluorescent.Maybe because<br />
we use weak RBS and GFP with LVA, the GFP protein degradation is too soon to be observed. <br />
Our project focus on T7 RNA Polymerase pulse and the output GFP will be amplified. So we <br />
hope there will be GFP pulse generated when co-transform plasmid 1 and 2.</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-28T00:44:04Z
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<font size=6>Version 1 :</font><br />
===<font size="5">Requirement of realizing genetic double click system</font>===<br />
-----<br />
1. T7 RNAP pulse in response to 1st and 2nd input<br><br />
In response to 1st and 2nd input, T7 RNAP has to express as pulse.<br><br />
To accomplish this, Lux/CI434 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part (Data shown below).<br><br />
<br><br />
2. CI repression<br><br />
CI needs to work as a repressor of T7/CI-OR1 hybrid promoter at 1st input and needs to<br />
have degradated before 2nd input not to work as a repressor at 2nd input.<br />
To accomplish this, T7/CI-OR1 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part (Data shown below).<br><br />
<br><br />
3. Setting the fixed time<br><br />
There is the fixed time between 1st and 2nd input.<br />
To accomplish this, CI has to re-express after washout. (Data not shown)<br />
<br />
===<font size="4">1-1. Lux/CI434 hybrid promoter</font>===<br />
-----<br />
===<font size="4">In detail</font>===<br />
-----<br />
Hybrid promoter Design <br />
<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
<br />
===<font size="4">design</font>===<br />
--------<br />
1. pLux/cI434 hybrid promoter <br />
<br />
Rationale <br />
---------<br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) at downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation. <br />
<br />
<br><br><br><br />
<br />
===<font size="4">References and links</font>===<br />
-----<br />
[1] http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
[2] http://partsregistry.org/Part:BBa_I12040<br />
[3] Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal <br><br />
control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101:<br><br />
6355-6360.<br />
<br />
===<font size="4">1-2. Pulse generator</font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396006 BBa_K396006]<br />
[[Image:名称未設定.png]] <br />
===<font size="4">2. T7/CI-OR1 hybrid promoter </font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000 BBa_K396000]<br />
T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.<br />
<br />
===<font size="3"> Checking the parts</font>===<br />
----<br />
[[Image:CI-1.jpg|frame|center|Fig. How to check the parts]]<br />
[[Image:CI-2.jpg|frame|center|Fig. Data: Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation.]]<br />
<br />
===<font size="3">Reference</font>===<br />
----<br />
Dubendorf, J.W. & Studier, F.W. Controlling basal expression in an<br />
inducible T7 expression system by blocking the target T7 promoter with<br />
lac repressor. Journal of Molecular Biology 219, 45-59 (1991).</div>
Lily.ch
http://2010.igem.org/File:%E5%90%8D%E7%A7%B0%E6%9C%AA%E8%A8%AD%E5%AE%9A.png
File:名称未設定.png
2010-10-28T00:38:47Z
<p>Lily.ch: </p>
<hr />
<div></div>
Lily.ch
http://2010.igem.org/File:Pulse.png
File:Pulse.png
2010-10-28T00:34:40Z
<p>Lily.ch: </p>
<hr />
<div></div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-27T23:26:43Z
<p>Lily.ch: /* design */</p>
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<br><br><br><br><br><br><br />
<font size=6>System 1 :</font><br />
===<font size="5">Requirement of realizing genetic double click system</font>===<br />
-----<br />
1. T7 RNAP pulse in response to 1st and 2nd input<br><br />
In response to 1st and 2nd input, T7 RNAP has to express as pulse.<br><br />
To accomplish this, Lux/CI434 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part (Data shown below).<br><br />
<br><br />
2. CI repression<br><br />
CI needs to work as a repressor of T7/CI-OR1 hybrid promoter at 1st input and needs to<br />
have degradated before 2nd input not to work as a repressor at 2nd input.<br />
To accomplish this, T7/CI-OR1 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part (Data shown below).<br><br />
<br><br />
3. Setting the fixed time<br><br />
There is the fixed time between 1st and 2nd input.<br />
To accomplish this, CI has to re-express after washout. (Data not shown)<br />
<br />
===<font size="4">1-1. Lux/CI434 hybrid promoter</font>===<br />
-----<br />
===<font size="4">In detail</font>===<br />
-----<br />
Hybrid promoter Design <br />
<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
<br />
===<font size="4">design</font>===<br />
--------<br />
1. pLux/cI434 hybrid promoter <br />
<br />
Rationale <br />
---------<br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) at downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation. <br />
<br />
<br><br><br><br />
<br />
===<font size="4">References and links</font>===<br />
-----<br />
[1] http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
[2] http://partsregistry.org/Part:BBa_I12040<br />
[3] Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal <br><br />
control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101:<br><br />
6355-6360.<br />
<br />
===<font size="4">1-2. Pulse generator</font>===<br />
-----<br />
<br />
===<font size="4">2. T7/CI-OR1 hybrid promoter </font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000 BBa_K396000]<br />
T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.<br />
<br />
===<font size="3"> Checking the parts</font>===<br />
----<br />
[[Image:CI-1.jpg|frame|center|Fig. How to check the parts]]<br />
[[Image:CI-2.jpg|frame|center|Fig. Data: Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation.]]<br />
<br />
===<font size="3">Reference</font>===<br />
----<br />
Dubendorf, J.W. & Studier, F.W. Controlling basal expression in an<br />
inducible T7 expression system by blocking the target T7 promoter with<br />
lac repressor. Journal of Molecular Biology 219, 45-59 (1991).</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-27T23:25:15Z
<p>Lily.ch: </p>
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display:block;<br />
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<font size=6>System 1 :</font><br />
===<font size="5">Requirement of realizing genetic double click system</font>===<br />
-----<br />
1. T7 RNAP pulse in response to 1st and 2nd input<br><br />
In response to 1st and 2nd input, T7 RNAP has to express as pulse.<br><br />
To accomplish this, Lux/CI434 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part (Data shown below).<br><br />
<br><br />
2. CI repression<br><br />
CI needs to work as a repressor of T7/CI-OR1 hybrid promoter at 1st input and needs to<br />
have degradated before 2nd input not to work as a repressor at 2nd input.<br />
To accomplish this, T7/CI-OR1 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part (Data shown below).<br><br />
<br><br />
3. Setting the fixed time<br><br />
There is the fixed time between 1st and 2nd input.<br />
To accomplish this, CI has to re-express after washout. (Data not shown)<br />
<br />
===<font size="4">1-1. Lux/CI434 hybrid promoter</font>===<br />
-----<br />
===<font size="4">In detail</font>===<br />
-----<br />
Hybrid promoter Design <br />
<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
<br />
===<font size="4">design</font>===<br />
--------<br />
1. pLux/cI434 hybrid promoter <br />
<br />
Rationale <br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation. <br />
<br />
<br><br><br><br />
<br />
===<font size="4">References and links</font>===<br />
-----<br />
[1] http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
[2] http://partsregistry.org/Part:BBa_I12040<br />
[3] Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal <br><br />
control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101:<br><br />
6355-6360.<br />
<br />
===<font size="4">1-2. Pulse generator</font>===<br />
-----<br />
<br />
===<font size="4">2. T7/CI-OR1 hybrid promoter </font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000 BBa_K396000]<br />
T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.<br />
<br />
===<font size="3"> Checking the parts</font>===<br />
----<br />
[[Image:CI-1.jpg|frame|center|Fig. How to check the parts]]<br />
[[Image:CI-2.jpg|frame|center|Fig. Data: Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation.]]<br />
<br />
===<font size="3">Reference</font>===<br />
----<br />
Dubendorf, J.W. & Studier, F.W. Controlling basal expression in an<br />
inducible T7 expression system by blocking the target T7 promoter with<br />
lac repressor. Journal of Molecular Biology 219, 45-59 (1991).</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Result
Team:Chiba/System 1/Result
2010-10-27T23:21:40Z
<p>Lily.ch: /* 1-1. Lux/CI434 hybrid promoter */</p>
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<br><br><br><br><br><br><br />
<font size=6>System 1 :</font><br />
===<font size="5">Requirement of realizing genetic double click system</font>===<br />
-----<br />
1. T7 RNAP pulse in response to 1st and 2nd input<br><br />
In response to 1st and 2nd input, T7 RNAP has to express as pulse.<br><br />
To accomplish this, Lux/CI434 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part (Data shown below).<br><br />
<br><br />
2. CI repression<br><br />
CI needs to work as a repressor of T7/CI-OR1 hybrid promoter at 1st input and needs to<br />
have degradated before 2nd input not to work as a repressor at 2nd input.<br />
To accomplish this, T7/CI-OR1 hybrid promoter should be activated or repressed stringently.<br><br />
So, we checked this part (Data shown below).<br><br />
<br><br />
3. Setting the fixed time<br><br />
There is the fixed time between 1st and 2nd input.<br />
To accomplish this, CI has to re-express after washout. (Data not shown)<br />
<br />
===<font size="4">1-1. Lux/CI434 hybrid promoter</font>===<br />
-----<br />
===<font size="5">In detail</font>===<br />
-----<br />
Hybrid promoter Design <br />
<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
<br />
===<font size="5">design</font>===<br />
--------<br />
1. pLux/cI434 hybrid promoter <br />
<br />
Rationale <br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation. <br />
<br />
<br><br><br><br />
<br />
===<font size="5">References and links</font>===<br />
-----<br />
[1] http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
[2] http://partsregistry.org/Part:BBa_I12040<br />
[3] Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal <br><br />
control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101:<br><br />
6355-6360.<br />
<br />
===<font size="4">1-2. Pulse generator</font>===<br />
-----<br />
<br />
===<font size="4">2. T7/CI-OR1 hybrid promoter </font>===<br />
-----<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000 BBa_K396000]<br />
T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.<br />
<br />
===<font size="3"> Checking the parts</font>===<br />
----<br />
[[Image:CI-1.jpg|frame|center|Fig. How to check the parts]]<br />
[[Image:CI-2.jpg|frame|center|Fig. Data: Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation.]]<br />
<br />
===<font size="3">Reference</font>===<br />
----<br />
Dubendorf, J.W. & Studier, F.W. Controlling basal expression in an<br />
inducible T7 expression system by blocking the target T7 promoter with<br />
lac repressor. Journal of Molecular Biology 219, 45-59 (1991).</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-27T23:21:01Z
<p>Lily.ch: </p>
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<font size=6>System 1 :</font><br />
===<font size="5">Testing individual parts</font>===<br />
-----<br />
To realize the genetic double click system, we made two plasmids. First one is “GFP generator”, which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. Second one is “Pulse generator”, which generate the pulse of T7 RNAP in response to the 1st and 2nd input.<br />
The subparts of those plasmids are shown below.<br><br />
<center><br />
[[Image:Chiba_icon_1.jpg]]<br><br />
[[Image:Chiba_icon_2.jpg]]<br><br />
</center><br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
For GFP generator, the parts shown below were checked.<br />
<br />
1. Constitutive promoter<br><br />
2. LuxR<br> <br />
3. T7/CI-OR1 hybrid promoter<br />
<br />
<br />
For Pulse generator, the parts shown below were checked.<br />
<br />
1. Lux/CI434 hybrid promoter<br> <br />
2. Pulse generator<br />
<br />
Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
<br><br><br></div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Construction_Process
Team:Chiba/System 1/Construction Process
2010-10-27T23:18:08Z
<p>Lily.ch: /* Construction process of Pulse generator plasmid */</p>
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background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs9 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs9 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 a:hover span {<br />
background-position:100% -42px;<br />
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<br />
#tabs9 #current a {<br />
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#tabs9 #current a span {<br />
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--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs9"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
</div><br />
</body><br />
<!--- 2nd tab End---><br />
<!--- 3rd tab---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
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font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
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margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
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<br />
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border: none;<br />
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<br />
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}<br />
#tabs8 a span {<br />
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display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs8 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs8 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs8 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs8 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs8"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Testing_components"><span>Testing</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Construction_Process"><span>Construction</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Remarks"><span>Remarks</span></a></li><br />
</ul><br />
</div><br />
</body><br />
</html><br />
<!--- 3rd tab End ---><br />
<!--- Background Contents End ---><br />
<!-- -------------------------------------------------------------------------------<br />
-------------------------------------------------------------------------------<br />
ここから下を編集<br />
-------------------------------------------------------------------------------<br />
----------------------------------------------------------------------------------- --><br />
<br><br><br><br><br><br><br />
<font size=6>System 1 :</font><br />
===<font size="4">Construction process of GFP generator plasmid</font>===<br />
-----<br />
<br><br><br><center><br />
[[Image:Chiba_gfpgener.jpg]]</center><br />
<br><br><br><br />
<br />
===<font size="4">Construction process of Pulse generator plasmid</font>===<br />
-----<br />
<br><br><br><center><br />
[[Image: Construction.png]]</center><br />
<br><br><br></div>
Lily.ch
http://2010.igem.org/File:Construction.png
File:Construction.png
2010-10-27T23:14:25Z
<p>Lily.ch: uploaded a new version of "Image:Construction.png"</p>
<hr />
<div></div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Construction_Process
Team:Chiba/System 1/Construction Process
2010-10-27T23:09:33Z
<p>Lily.ch: </p>
<hr />
<div><!-- ---------------------------------------------------------<br />
--------------------------------------------------------------<br />
下の方に「ここから下を編集」というところがあります<br />
そっちを編集してください<br />
--------------------------------------------------------------<br />
------------------------------------------------------------ --><br />
<html><link rel="stylesheet" href="https://2010.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
<html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
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padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<!--- 1st tab ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
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margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
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<br />
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<br />
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display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
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color:#FFF;<br />
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/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
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#tabs10 #current a span {<br />
background-position:100% -42px;<br />
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--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- 1st tab End ---><br />
<!--- 2nd tab ---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
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margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
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<br />
/*- Menu Tabs 9--------------------------- */<br />
<br />
#tabs9 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#125FDB;<br />
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margin-left:40px;<br />
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background:url("tableft10.gif") no-repeat left top;<br />
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}<br />
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float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs9 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs9 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs9 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs9"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
</div><br />
</body><br />
<!--- 2nd tab End---><br />
<!--- 3rd tab---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
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margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
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<br />
img {<br />
border: none;<br />
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<br />
/*- Menu Tabs 8--------------------------- */<br />
<br />
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}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs8 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs8 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs8 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs8 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs8"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Testing_components"><span>Testing</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Construction_Process"><span>Construction</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Remarks"><span>Remarks</span></a></li><br />
</ul><br />
</div><br />
</body><br />
</html><br />
<!--- 3rd tab End ---><br />
<!--- Background Contents End ---><br />
<!-- -------------------------------------------------------------------------------<br />
-------------------------------------------------------------------------------<br />
ここから下を編集<br />
-------------------------------------------------------------------------------<br />
----------------------------------------------------------------------------------- --><br />
<br><br><br><br><br><br><br />
<font size=6>System 1 :</font><br />
===<font size="4">Construction process of GFP generator plasmid</font>===<br />
-----<br />
<br><br><br><center><br />
[[Image:Chiba_gfpgener.jpg]]</center><br />
<br><br><br><br />
<br />
===<font size="4">Construction process of Pulse generator plasmid</font>===<br />
-----<br />
<br><br><br><center><br />
[[Image:Construct.png]]</center><br />
<br><br><br></div>
Lily.ch
http://2010.igem.org/File:Construct.png
File:Construct.png
2010-10-27T23:07:27Z
<p>Lily.ch: </p>
<hr />
<div></div>
Lily.ch
http://2010.igem.org/File:Con.png
File:Con.png
2010-10-27T23:02:36Z
<p>Lily.ch: </p>
<hr />
<div></div>
Lily.ch
http://2010.igem.org/Team:Chiba/Member
Team:Chiba/Member
2010-10-27T22:58:01Z
<p>Lily.ch: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<!--- 1st tab ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
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padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
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<body><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our Members</font><br><br />
<hr width="500" size="1" align="left"> <br />
</td><br />
</tr><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"><br />
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<font size=2 face=verdana><br />
<td width="200"><img src="https://static.igem.org/mediawiki/2010/e/ea/Chiba_05_1.JPG"></td><br />
<td width="500"><font size=2 face=verdana><br />
Ryuji Yamamoto<br />
</td> <br />
</tr><br />
<tr><br />
<td width="200"><img src="https://static.igem.org/mediawiki/2010/d/d1/Chiba_Ling.jpg"></td><br />
<td width="500"><font size=2 face=verdana><br />
This is Ling Li, a graduate student from Chiba University. I participate in iGEM for the first <br />
time and I see a great opportunity and future in synthetic biology. My major is applied chemistry<br />
but I have great interest in molecular biology. Hope we have the chance to get together! <br />
</td> <br />
</tr><br />
<tr><br />
<td width="200"><img src="https://static.igem.org/mediawiki/2010/a/ad/Chiba_Onoda.jpg"></td><br />
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Hello,My name is Manabu Onoda.I'm an undergraduate student at Chiba University in Japan.<br />
</td> <br />
</tr> <br />
<tr><br />
<td width="200"><img src="https://static.igem.org/mediawiki/2010/d/d0/Chiba_04.JPG"></td><br />
<td width="500"><font size=2 face=verdana><br />
Kohei Ike<br />
</td> <br />
</tr> <br />
<tr><br />
<td width="200"><img src="https://static.igem.org/mediawiki/2010/3/39/Chiba_Lee.jpg"></td><br />
<td width="500"><font size=2 face=verdana><br />
Hello, I'm Gunhye Lee. I'm glad that participate in iGEM this year. <br />
I'm studying chemical engineering and very interested in biosynthesis.<br />
I think iGEM is great chance that can study our own, original and fun project, so I'm really excited.<br><br />
I like listening to music and I love sports, especially figure skating. <br />
Not as a skater, but as a fan though. <br />
My favorite skater is Yuna Kim, Olympic Champion.<br />
I'm watching her beautiful program everyday.<br><br />
See you soon in Boston.<br />
</td> <br />
</tr> <br />
<tr><br />
<td width="200"><img src="https://static.igem.org/mediawiki/2010/2/2d/Chiba_Jung.jpg"></td><br />
<td width="500"><font size=2 face=verdana><br />
Hello~Welcome to our wiki!!!<br> I'm Gyuwoon Jung. I'm undergraduate of Chiba University in Japan and a sophomore majoring in Department of Applied Chemistry & Biotechnology at Chiba University. I heard synthetic biology first time through iGEM. Potentiality of synthetic biology suprised me. I expect that this field will be developed.<br> So I am looking forward to you!!</td> <br />
</tr><br />
<tr><br />
<td width="200"><img src="https://static.igem.org/mediawiki/2010/2/2e/Chiba_03.jpg"></td><br />
<td width="500"><font size=2 face=verdana><br />
Hi! I’m Yuki Kawamura. I’m a freshman at a Chiba University in Japan. My major is horticulture. Horticulture is gardening, agriculture, and so on. In particular, I’m interested in microbiology.<br><br />
I’m into listening music, reading books, and playing a flute and a shamisen(Japanese instrument). My newest passion is history novel. I’m looking forward to iGEM2010!<br />
<br />
</td> <br />
</tr></table><br><br><br><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Advisors</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<font size=2 face=verdana><br />
Daisuke Umeno<br><br />
Maiko Furubayashi<br />
</td><br />
</tr><br />
</table><br />
</body><br />
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<!--- Main Contents End ---></div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-27T22:29:21Z
<p>Lily.ch: /* In detail */</p>
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<li><a href="https://2010.igem.org/Team:Chiba/System_1/Testing_components"><span>Testing</span></a></li><br />
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===<font size="5">Testing individual parts</font>===<br />
-----<br />
To realize the genetic double click system, we made two plasmids. First one is “GFP generator”, which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. Second one is “Pulse generator”, which generate the pulse of T7 RNAP in response to the 1st and 2nd input.<br />
The subparts of those plasmids are shown below.<br><br />
<center><br />
[[Image:Chiba_icon_1.jpg]]<br><br />
[[Image:Chiba_icon_2.jpg]]<br><br />
</center><br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
For GFP generator, the parts shown below were checked.<br />
<br />
1. Constitutive promoter<br><br />
2. LuxR<br> <br />
3. T7/CI-OR1 hybrid promoter<br />
<br />
<br />
For Pulse generator, the parts shown below were checked.<br />
<br />
1. Lux/CI434 hybrid promoter<br> <br />
2. Pulse generator<br />
<br />
Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
<br><br><br><br />
<br />
===<font size="5">In detail</font>===<br />
-----<br />
Hybrid promoter Design <br />
<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
<br />
===<font size="5">design</font>===<br />
--------<br />
1. pLux/cI434 hybrid promoter <br />
<br />
Rationale <br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation. <br />
<br />
<br><br><br><br />
<br />
===<font size="5">References and links</font>===<br />
-----<br />
[1] http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
[2] http://partsregistry.org/Part:BBa_I12040<br />
[3] Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal <br><br />
control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101:<br><br />
6355-6360.</div>
Lily.ch
http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-27T20:22:40Z
<p>Lily.ch: /* Testing individual parts */</p>
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<br><br><br><br />
===Testing individual parts===<br />
To realize the genetic double click system, we made two plasmids. First one is “GFP generator”, which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. Second one is “Pulse generator”, which generate the pulse of T7 RNAP in response to the 1st and 2nd input.<br />
The subparts of those plasmids are shown in Fig.1.<br />
<br />
<br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
For GFP generator, the parts shown below were checked.<br />
<br />
1. Constitutive promoter<br><br />
2. LuxR<br> <br />
3. T7/CI-OR1 hybrid promoter<br />
<br />
<br />
For Pulse generator, the parts shown below were checked.<br />
<br />
1. Lux/CI434 hybrid promoter<br> <br />
2. Pulse generator<br />
<br />
Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
===In detail===<br />
Hybrid promoter Design <br />
<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
<br />
About the design<br />
--------<br />
1. pLux/cI434 hybrid promoter <br />
<br />
Rationale <br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation. <br />
<br />
References and links <br />
----------<br />
[1] http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
[2] http://partsregistry.org/Part:BBa_I12040<br />
[3] Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal <br><br />
control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101:<br><br />
6355-6360.</div>
Lily.ch
http://2010.igem.org/Team:Chiba/parts/parts_status
Team:Chiba/parts/parts status
2010-10-27T09:26:19Z
<p>Lily.ch: </p>
<hr />
<div>==Parts Status(Parts created by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td><br />
<td width="300">pLux/cI434 Hybrid promoter</td><br />
<td width="200">61<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2133<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
===Hybrid promoter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 BBa_K396003]</td><br />
<td width="300">UV light GFP with LVA tag</td><br />
<td width="200">723<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2072<td><br />
<td width="200">2795<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
<br />
<br />
===Reporter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396001 BBa_K396001]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 pLux/cI434 hybrid promoter]+[http://partsregistry.org/Part:BBa_B0031 RBS weak]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 GFPuv LVA tag]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 Prom-luxR]</td><br />
<td width="200">814<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2886<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Generator===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396000 BBa_K396000]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396000 T7/CI Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396011 (composite with LuxR generator)]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Composite===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396006 BBa_K396006]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396001 pLux/cI434 Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396012 cI434 generator]</td><br />
<td width="200">1654<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">3726<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 BBa_K396007]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 T7 RNA Polymerase pulse generator]</td><br />
<td width="200">3542<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">5614<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
===Device===<br />
<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td><br />
<td width="300">Promoter (HSL-mediated luxR repressor)</td><br />
<td width="200">30<td><br />
<td width="200">pSB2K3<td><br />
<td width="200">F and P1 lytic<td><br />
<td width="200">4425<td><br />
<td width="200">4455<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td><br />
<td width="300">ptet+cI</td><br />
<td width="200">992<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3071<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
</table><br />
<br />
===Parts Status(Parts used by Chiba)===</div>
Lily.ch
http://2010.igem.org/Team:Chiba/parts/parts_status
Team:Chiba/parts/parts status
2010-10-26T19:13:43Z
<p>Lily.ch: New page: ==Parts Status(Parts created by Chiba)== ===Hybrid promoter=== <table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center> <tr> <td width="200">Part Name</...</p>
<hr />
<div>==Parts Status(Parts created by Chiba)==<br />
<br />
===Hybrid promoter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td><br />
<td width="300">pLux/cI434 Hybrid promoter</td><br />
<td width="200">61<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2133<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Reporter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 BBa_K396003]</td><br />
<td width="300">UV light GFP with LVA tag</td><br />
<td width="200">723<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2072<td><br />
<td width="200">2795<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Generator===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396001 BBa_K396001]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 pLux/cI434 hybrid promoter]+[http://partsregistry.org/Part:BBa_B0031 RBS weak]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 GFPuv LVA tag]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 Prom-luxR]</td><br />
<td width="200">814<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2886<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Composite===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396000 BBa_K396000]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396000 T7/CI Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396011 (composite with LuxR generator)]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Device===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396006 BBa_K396006]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396001 pLux/cI434 Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396012 cI434 generator]</td><br />
<td width="200">1654<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">3726<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 BBa_K396007]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 T7 RNA Polymerase pulse generator]</td><br />
<td width="200">3542<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">5614<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td><br />
<td width="300">Promoter (HSL-mediated luxR repressor)</td><br />
<td width="200">30<td><br />
<td width="200">pSB2K3<td><br />
<td width="200">F and P1 lytic<td><br />
<td width="200">4425<td><br />
<td width="200">4455<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td><br />
<td width="300">ptet+cI</td><br />
<td width="200">992<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3071<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr></div>
Lily.ch
http://2010.igem.org/Team:Chiba/Parts/test
Team:Chiba/Parts/test
2010-10-26T19:09:59Z
<p>Lily.ch: /* Reporters */</p>
<hr />
<div>==Parts Status(Parts created by Chiba)==<br />
<br />
===Hybrid promoter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td><br />
<td width="300">pLux/cI434 Hybrid promoter</td><br />
<td width="200">61<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2133<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Reporter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 BBa_K396003]</td><br />
<td width="300">UV light GFP with LVA tag</td><br />
<td width="200">723<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2072<td><br />
<td width="200">2795<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Generator===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396001 BBa_K396001]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 pLux/cI434 hybrid promoter]+[http://partsregistry.org/Part:BBa_B0031 RBS weak]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 GFPuv LVA tag]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 Prom-luxR]</td><br />
<td width="200">814<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2886<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Composite===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396000 BBa_K396000]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396000 T7/CI Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396011 (composite with LuxR generator)]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Device===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396006 BBa_K396006]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396001 pLux/cI434 Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396012 cI434 generator]</td><br />
<td width="200">1654<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">3726<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 BBa_K396007]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 T7 RNA Polymerase pulse generator]</td><br />
<td width="200">3542<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">5614<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td><br />
<td width="300">Promoter (HSL-mediated luxR repressor)</td><br />
<td width="200">30<td><br />
<td width="200">pSB2K3<td><br />
<td width="200">F and P1 lytic<td><br />
<td width="200">4425<td><br />
<td width="200">4455<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td><br />
<td width="300">ptet+cI</td><br />
<td width="200">992<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3071<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr></div>
Lily.ch
http://2010.igem.org/Team:Chiba/Parts/test
Team:Chiba/Parts/test
2010-10-26T19:08:18Z
<p>Lily.ch: </p>
<hr />
<div>==Parts Status(Parts created by Chiba)==<br />
<br />
===Hybrid promoter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td><br />
<td width="300">pLux/cI434 Hybrid promoter</td><br />
<td width="200">61<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2133<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Reporters===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 BBa_K396003]</td><br />
<td width="300">UV light GFP with LVA tag</td><br />
<td width="200">723<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2072<td><br />
<td width="200">2795<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Generator===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396001 BBa_K396001]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 pLux/cI434 hybrid promoter]+[http://partsregistry.org/Part:BBa_B0031 RBS weak]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 GFPuv LVA tag]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 Prom-luxR]</td><br />
<td width="200">814<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2886<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Composite===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396000 BBa_K396000]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396000 T7/CI Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396011 (composite with LuxR generator)]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Device===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396006 BBa_K396006]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396001 pLux/cI434 Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396012 cI434 generator]</td><br />
<td width="200">1654<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">3726<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 BBa_K396007]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 T7 RNA Polymerase pulse generator]</td><br />
<td width="200">3542<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">5614<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td><br />
<td width="300">Promoter (HSL-mediated luxR repressor)</td><br />
<td width="200">30<td><br />
<td width="200">pSB2K3<td><br />
<td width="200">F and P1 lytic<td><br />
<td width="200">4425<td><br />
<td width="200">4455<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td><br />
<td width="300">ptet+cI</td><br />
<td width="200">992<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3071<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr></div>
Lily.ch
http://2010.igem.org/Team:Chiba/Parts
Team:Chiba/Parts
2010-10-26T19:05:18Z
<p>Lily.ch: /* Parts */</p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="white" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 1 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
/*- Menu Tabs E--------------------------- */<br />
<br />
#tabsE {<br />
float:left;<br />
width:100%;<br />
background:#373737;<br />
font-size:100%;<br />
line-height:normal;<br />
margin-top: -9px;<br />
<br />
}<br />
#tabsE ul {<br />
margin:0;<br />
padding:2px 10px 0 95px;<br />
list-style:none;<br />
}<br />
#tabsE li {<br />
float:left;<br />
display:inline;<br />
padding:0;<br />
margin-right: 40px;<br />
<br />
}<br />
#tabsE li ul {<br />
position: absolute;<br />
left: -999em;<br />
}<br />
<br />
#tabsE li:hover ul {<br />
float:left;<br />
color: white !important;<br />
<br />
}<br />
<br />
#tabsE li:hover ul, #tabsE li.sfhover ul {<br />
left:auto;<br />
padding: 10px;<br />
color: white !important;<br />
}<br />
<br />
#tabsE a {<br />
float:left;<br />
background:url("tableftE.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabsE a span {<br />
float:left;<br />
display:block;<br />
background:url("tabrightE.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabsE a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabsE a:hover span {<br />
color:#FFF;<br />
}<br />
#tabsE a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabsE a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabsE #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabsE #current a span {<br />
background-position:100% -42px;<br />
}<br />
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if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabsE"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a><br />
<ul style="z-index:1"> <br><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Team">Team</a></li> <li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Member">Member</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/School">School</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a><br />
<ul style="z-index:1"><br><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Project">Overall Project</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Circuit_1">Circuit 1</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Circuit_2">Circuit 2</a></li><br />
</ul><br />
</li> <br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Result"><span>Result</span></a><br />
<ul style="z-index:1"><br><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Plasmid1">Plasmid 1</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Plasmid2">Plasmid 2</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/lux_promoter">lux Promoter</a></li><br><br />
</ul><br />
</li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a><br />
<ul style="z-index:1"><br><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Notebook">Notebook 1</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Notebook_2">Notebook 2</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Notebook_3">Notebook 3</a></li><br><br />
</ul><br />
</li><br />
<br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
</body><br />
</html><br />
<!--- Background Contents End ---><br />
<!--- Main Contents Starts ---><br />
<br><br><br><br />
===Parts===<br />
<br />
New for iGEM 2010 is the ''groupparts'' tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.<br />
<br />
<groupparts>iGEM010 Chiba</groupparts><br />
==== [[Team:Chiba/parts/parts status|parts status]] ====</div>
Lily.ch
http://2010.igem.org/Team:Chiba/Parts/test
Team:Chiba/Parts/test
2010-10-26T18:56:59Z
<p>Lily.ch: </p>
<hr />
<div><html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
[[Image:Chiba-U.gif|center]]<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Results|Result]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/support|support]]<br />
|}<br />
{| align="justify"<br />
|<br />
==Parts Status(Parts created by Chiba)==<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Results|Result]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/support|support]]<br />
|}<br />
<br />
===Hybrid promoter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td><br />
<td width="300">pLux/cI434 Hybrid promoter</td><br />
<td width="200">61<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2133<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Reporters===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 BBa_K396003]</td><br />
<td width="300">UV light GFP with LVA tag</td><br />
<td width="200">723<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2072<td><br />
<td width="200">2795<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Generator===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396001 BBa_K396001]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 pLux/cI434 hybrid promoter]+[http://partsregistry.org/Part:BBa_B0031 RBS weak]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 GFPuv LVA tag]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 Prom-luxR]</td><br />
<td width="200">814<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2886<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Composite===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396000 BBa_K396000]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396000 T7/CI Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396011 (composite with LuxR generator)]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
===Device===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396006 BBa_K396006]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_K396001 pLux/cI434 Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396012 cI434 generator]</td><br />
<td width="200">1654<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">3726<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 BBa_K396007]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 T7 RNA Polymerase pulse generator]</td><br />
<td width="200">3542<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">5614<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td><br />
<td width="300">Promoter (HSL-mediated luxR repressor)</td><br />
<td width="200">30<td><br />
<td width="200">pSB2K3<td><br />
<td width="200">F and P1 lytic<td><br />
<td width="200">4425<td><br />
<td width="200">4455<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td><br />
<td width="300">ptet+cI</td><br />
<td width="200">992<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3071<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr></div>
Lily.ch
http://2010.igem.org/Team:Chiba/Parts/test
Team:Chiba/Parts/test
2010-10-26T18:40:45Z
<p>Lily.ch: </p>
<hr />
<div><html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
[[Image:Chiba-U.gif|center]]<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Results|Result]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/support|support]]<br />
|}<br />
{| align="justify"<br />
|<br />
==Parts Status(Parts created by Chiba)==<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Results|Result]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/support|support]]<br />
|}<br />
<br />
===Hybrid promoter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td><br />
<td width="300">pLux/cI434 Hybrid promoter</td><br />
<td width="200">61<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2133<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Reporters===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 BBa_K396003]</td><br />
<td width="300">UV light GFP with LVA tag</td><br />
<td width="200">723<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2072<td><br />
<td width="200">2795<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Generator===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396001 BBa_K396001]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 pLux/cI434 hybrid promoter]+[http://partsregistry.org/Part:BBa_B0031 RBS weak]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 GFPuv LVA tag]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 Prom-luxR]</td><br />
<td width="200">814<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2886<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
<br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td><br />
<td width="300">Promoter (HSL-mediated luxR repressor)</td><br />
<td width="200">30<td><br />
<td width="200">pSB2K3<td><br />
<td width="200">F and P1 lytic<td><br />
<td width="200">4425<td><br />
<td width="200">4455<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td><br />
<td width="300">ptet+cI</td><br />
<td width="200">992<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3071<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr></div>
Lily.ch
http://2010.igem.org/Team:Chiba/Parts/test
Team:Chiba/Parts/test
2010-10-26T18:32:17Z
<p>Lily.ch: /* Hybrid promoter */</p>
<hr />
<div><html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
[[Image:Chiba-U.gif|center]]<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Reference|Reference]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]<br />
|}<br />
{| align="justify"<br />
|<br />
==Parts Status(Parts created by Chiba)==<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Reference|Reference]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]<br />
|}<br />
<br />
<br />
===Hybrid promoter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
<tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td><br />
<td width="300">pLux/cI434 Hybrid promoter</td><br />
<td width="200">61<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2133<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Reporters===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 BBa_K396003]</td><br />
<td width="300">UV light GFP with LVA tag</td><br />
<td width="200">723<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2072<td><br />
<td width="200">2795<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Generator===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
<br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396001 BBa_K396001]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 pLux/cI434 hybrid promoter]+[http://partsregistry.org/Part:BBa_B0031 RBS weak]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 GFPuv LVA tag]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
<br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 Prom-luxR]</td><br />
<td width="200">814<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2886<td><br />
<td width="200">Cm<td><br />
<td width="350">80/miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
<br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td><br />
<td width="300">Promoter (HSL-mediated luxR repressor)</td><br />
<td width="200">30<td><br />
<td width="200">pSB2K3<td><br />
<td width="200">F and P1 lytic<td><br />
<td width="200">4425<td><br />
<td width="200">4455<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td><br />
<td width="300">ptet+cI</td><br />
<td width="200">992<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3071<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<br />
===Receiver protein===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td><br />
<td width="300">ptet+rbs+RhlR</td><br />
<td width="200">867<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2946<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td><br />
<td width="300">ptet+rbs+RhlR</td><br />
<td width="200">867<td><br />
<td width="200">pSB3K3<td><br />
<td width="200">p15A<td><br />
<td width="200">2750<td><br />
<td width="200">3617<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td><br />
<td width="300">ptet+rbs+LasR</td><br />
<td width="200">861<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2940<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td><br />
<td width="300">ptet+rbs+LasR</td><br />
<td width="200">861<td><br />
<td width="200">pSB3K3<td><br />
<td width="200">p15A<td><br />
<td width="200">2750<td><br />
<td width="200">3611<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td><br />
<td width="300">ptet+rbs+CinR</td><br />
<td width="200">867<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2946<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td><br />
<td width="300">ptet+rbs+CinR</td><br />
<td width="200">867<td><br />
<td width="200">pSB3K3<td><br />
<td width="200">p15A<td><br />
<td width="200">2750<td><br />
<td width="200">3617<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
</table><br />
<br />
===Reporters===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084002 BBa_K084002]</td><br />
<td width="300">rbs+venusYFP(yeast optimized)</td><br />
<td width="200">767<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2079<td><br />
<td width="200">3915<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">no<td><br />
<td width="50">no<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084003 BBa_K084003]</td><br />
<td width="300">ptet+LuxR+plux+venusYFP(yeast optimized)</td><br />
<td width="200">1836<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3915<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
</table><br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]</td><br />
<td width="300">ptet+LuxR+plux+GFP</td><br />
<td width="200">1945<td><br />
<td width="200">pSB1A3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2257<td><br />
<td width="200">4102<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]</td><br />
<td width="300">ptet+LuxI</td><br />
<td width="200">723<td><br />
<td width="200">pSB1AC3<td><br />
<td width="200">pMB1<td><br />
<td width="200">3005<td><br />
<td width="200">3778<td><br />
<td width="200">Amp/Cm<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
</table><br />
<br />
<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Reference|Reference]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]<br />
|}</div>
Lily.ch
http://2010.igem.org/Team:Chiba/Parts/test
Team:Chiba/Parts/test
2010-10-26T18:31:13Z
<p>Lily.ch: /* Autoinducer synthase */</p>
<hr />
<div><html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
[[Image:Chiba-U.gif|center]]<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Reference|Reference]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]<br />
|}<br />
{| align="justify"<br />
|<br />
==Parts Status(Parts created by Chiba)==<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Reference|Reference]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]<br />
|}<br />
<br />
<br />
===Hybrid promoter===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
<tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002]</td><br />
<td width="300">pLux/cI434 Hybrid promoter</td><br />
<td width="200">61<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2133<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Reporters===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 BBa_K396003]</td><br />
<td width="300">UV light GFP with LVA tag</td><br />
<td width="200">723<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2072<td><br />
<td width="200">2795<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">no<td><br />
</tr><br />
<br />
===Generator===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
<br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K396001 BBa_K396001]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 pLux/cI434 hybrid promoter]+[http://partsregistry.org/Part:BBa_B0031 RBS weak]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 GFPuv LVA tag]</td><br />
<td width="200">806<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2878<td><br />
<td width="200">Cm<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
<br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011]</td><br />
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 Prom-luxR]</td><br />
<td width="200">814<td><br />
<td width="200">pSB1C3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2072<td><br />
<td width="200">2886<td><br />
<td width="200">Cm<td><br />
<td width="350">80/miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">ok<td><br />
</tr><br />
<br />
<br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td><br />
<td width="300">Promoter (HSL-mediated luxR repressor)</td><br />
<td width="200">30<td><br />
<td width="200">pSB2K3<td><br />
<td width="200">F and P1 lytic<td><br />
<td width="200">4425<td><br />
<td width="200">4455<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td><br />
<td width="300">ptet+cI</td><br />
<td width="200">992<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3071<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<br />
===Receiver protein===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td><br />
<td width="300">ptet+rbs+RhlR</td><br />
<td width="200">867<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2946<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td><br />
<td width="300">ptet+rbs+RhlR</td><br />
<td width="200">867<td><br />
<td width="200">pSB3K3<td><br />
<td width="200">p15A<td><br />
<td width="200">2750<td><br />
<td width="200">3617<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td><br />
<td width="300">ptet+rbs+LasR</td><br />
<td width="200">861<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2940<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td><br />
<td width="300">ptet+rbs+LasR</td><br />
<td width="200">861<td><br />
<td width="200">pSB3K3<td><br />
<td width="200">p15A<td><br />
<td width="200">2750<td><br />
<td width="200">3611<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td><br />
<td width="300">ptet+rbs+CinR</td><br />
<td width="200">867<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2946<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td><br />
<td width="300">ptet+rbs+CinR</td><br />
<td width="200">867<td><br />
<td width="200">pSB3K3<td><br />
<td width="200">p15A<td><br />
<td width="200">2750<td><br />
<td width="200">3617<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
</table><br />
<br />
===Reporters===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084002 BBa_K084002]</td><br />
<td width="300">rbs+venusYFP(yeast optimized)</td><br />
<td width="200">767<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2079<td><br />
<td width="200">3915<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">no<td><br />
<td width="50">no<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084003 BBa_K084003]</td><br />
<td width="300">ptet+LuxR+plux+venusYFP(yeast optimized)</td><br />
<td width="200">1836<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3915<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
</table><br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]</td><br />
<td width="300">ptet+LuxR+plux+GFP</td><br />
<td width="200">1945<td><br />
<td width="200">pSB1A3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2257<td><br />
<td width="200">4102<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]</td><br />
<td width="300">ptet+LuxI</td><br />
<td width="200">723<td><br />
<td width="200">pSB1AC3<td><br />
<td width="200">pMB1<td><br />
<td width="200">3005<td><br />
<td width="200">3778<td><br />
<td width="200">Amp/Cm<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
</table><br />
<br />
<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Reference|Reference]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]<br />
|}</div>
Lily.ch
http://2010.igem.org/Team:Chiba/Parts/test
Team:Chiba/Parts/test
2010-10-26T17:56:45Z
<p>Lily.ch: New page: <html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html> center {| style...</p>
<hr />
<div><html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
[[Image:Chiba-U.gif|center]]<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Reference|Reference]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]<br />
|}<br />
{| align="justify"<br />
|<br />
==Parts Status(Parts created by Chiba)==<br />
===Autoinducer synthase===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
<br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)]</td><br />
<td width="200">835<td><br />
<td width="200">pSB1AK3<td><br />
<td width="200">pMB1<td><br />
<td width="200">3189<td><br />
<td width="200">4024<td><br />
<td width="200">Amp/Kan<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
<br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]</td><br />
<td width="200">835<td><br />
<td width="200">pSB1AK3<td><br />
<td width="200">pMB1<td><br />
<td width="200">3189<td><br />
<td width="200">4024<td><br />
<td width="200">Amp/Kan<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI]</td><br />
<td width="200">893<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2972<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084010 BBa_K084010]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9030 rbs+CinI]</td><br />
<td width="200">953<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3032<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084012 BBa_K084012]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 LuxI(no LVA)]</td><br />
<td width="200">811<td><br />
<td width="200">pSB1AK3<td><br />
<td width="200">pMB1<td><br />
<td width="200">3189<td><br />
<td width="200">4000<td><br />
<td width="200">Amp/Kan<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084014 BBa_K084014]</td><br />
<td width="300">[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0261 rbs+LuxI]</td><br />
<td width="200">869<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2948<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
</table><br />
<br />
===Receiver protein===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td><br />
<td width="300">ptet+rbs+RhlR</td><br />
<td width="200">867<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2946<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td><br />
<td width="300">ptet+rbs+RhlR</td><br />
<td width="200">867<td><br />
<td width="200">pSB3K3<td><br />
<td width="200">p15A<td><br />
<td width="200">2750<td><br />
<td width="200">3617<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td><br />
<td width="300">ptet+rbs+LasR</td><br />
<td width="200">861<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2940<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td><br />
<td width="300">ptet+rbs+LasR</td><br />
<td width="200">861<td><br />
<td width="200">pSB3K3<td><br />
<td width="200">p15A<td><br />
<td width="200">2750<td><br />
<td width="200">3611<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td><br />
<td width="300">ptet+rbs+CinR</td><br />
<td width="200">867<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">2946<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">ok<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td><br />
<td width="300">ptet+rbs+CinR</td><br />
<td width="200">867<td><br />
<td width="200">pSB3K3<td><br />
<td width="200">p15A<td><br />
<td width="200">2750<td><br />
<td width="200">3617<td><br />
<td width="200">Kan<td><br />
<td width="350">miniprepped<td><br />
<td width="50">Not yet<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
</table><br />
<br />
===Reporters===<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
<td width="200">Sent<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084002 BBa_K084002]</td><br />
<td width="300">rbs+venusYFP(yeast optimized)</td><br />
<td width="200">767<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1 <td><br />
<td width="200">2079<td><br />
<td width="200">3915<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">no<td><br />
<td width="50">no<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_K084003 BBa_K084003]</td><br />
<td width="300">ptet+LuxR+plux+venusYFP(yeast optimized)</td><br />
<td width="200">1836<td><br />
<td width="200">pSB1A2<td><br />
<td width="200">pMB1<td><br />
<td width="200">2079<td><br />
<td width="200">3915<td><br />
<td width="200">Amp<td><br />
<td width="350">miniprepped<td><br />
<td width="50">OK<td><br />
<td width="50">[[Image:Chiba check.png]]<td><br />
</tr><br />
</table><br />
==Parts Status(Parts used by Chiba)==<br />
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center><br />
<tr><br />
<td width="200">Part Name</td><br />
<td width="200">Sub Parts</td><br />
<td width="200">Part Length(bp)<td><br />
<td width="200">Plasmid Vector<td><br />
<td width="200">Origin<td><br />
<td width="300">Part Length(bp)<td><br />
<td width="200">Part and vector length(bp)<td><br />
<td width="200">Resistance<td><br />
<td width="200">Status<td><br />
<td width="200">Sequenced<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]</td><br />
<td width="300">ptet+LuxR+plux+GFP</td><br />
<td width="200">1945<td><br />
<td width="200">pSB1A3<td><br />
<td width="200">pMB1<td><br />
<td width="200">2257<td><br />
<td width="200">4102<td><br />
<td width="200">Amp<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
<tr><br />
<td width="200">[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]</td><br />
<td width="300">ptet+LuxI</td><br />
<td width="200">723<td><br />
<td width="200">pSB1AC3<td><br />
<td width="200">pMB1<td><br />
<td width="200">3005<td><br />
<td width="200">3778<td><br />
<td width="200">Amp/Cm<td><br />
<td width="350">Glycerol stock in -80/miniprepped<td><br />
<td width="50">OK<td><br />
</tr><br />
</table><br />
<br />
<br />
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |<br />
!align="center"|[[Team:Chiba|Home]]<br />
!align="center"|[[Team:Chiba/Team|The Team]]<br />
!align="center"|[[Team:Chiba/Project|The Project]]<br />
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Chiba/Reference|Reference]]<br />
!align="center"|[[Team:Chiba/Notebook|Notebook]]<br />
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]<br />
|}</div>
Lily.ch
http://2010.igem.org/Team:Chiba/test-1
Team:Chiba/test-1
2010-10-09T19:53:27Z
<p>Lily.ch: New page: <html> <img src="https://static.igem.org/mediawiki/2010/2/2a/Chiba_home2.jpg" width="965" height="230"> </html> <html> <p> <style type="text/css"> #allcontent { width: 950px; } bod...</p>
<hr />
<div><html><br />
<img src="https://static.igem.org/mediawiki/2010/2/2a/Chiba_home2.jpg" width="965" height="230"><br />
</html><br />
<html><br />
<p><br />
<style type="text/css"> <br />
<br />
#allcontent {<br />
width: 950px;<br />
}<br />
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float: right;<br />
width: 300px;<br />
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width: 300px;<br />
padding: 10px;<br />
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<!-- !END CHIBACSS --><br />
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<li><a class="bannertoplinks" href="#">Team</a><br />
<ul style="z-index:1"><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Team">Team</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Member">Member</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/School">School</a></li><br />
</ul><br />
</li><br />
<li><a class="bannertoplinks" href="#">Project</a><br />
<ul style="z-index:1"><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Project">test1 Project</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Circuit_1">test2</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Circuit_2">test3</a></li><br />
</ul><br />
</li> <br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Parts">Parts</a><br />
</li><br />
<br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Modeling">Modeling</a><br />
</li><br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Notebook">Notebook</a><br />
</li><br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Safety">Safety</a><br />
</li><br />
<br />
<br />
</center> <br />
</div><br />
</html><br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| border="0" cellpadding="50" cellspacing="0" align="center"<br />
|-<br />
|<font size=5>PROJECT DESCRIPTION</font><br />
<br><br />
----<br />
<br />
<font size=3><br />
We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
</font><br><br />
----<br />
<br />
<html> <div style="text-align:center;"> <br />
<object type="application/x-shockwave-flash" data="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=ffcc66&bgcolor=006699&date_month=11&date_day=05&date_year=0&un=2010 iGEM JAMBOREE&size=normal&mo=11&da=05&yr=2010" width="350" height="100"><param name="movie" value="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=00B32B&bgcolor=003366&date_month=11&date_day=05&date_year=0&un=NEXT IGEM JAMBOREE&size=normal&mo=11&da=05&yr=2010" /><param name="bgcolor" value="#474747" /></object><img src="http://www.oneplusyou.com/q/img/bb_badges/countdown.jpg" alt="" style="display: none;" height="1" width="1" /><div><br />
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<a href="http://chem.tf.chiba-u.jp/igem/"><img src="https://static.igem.org/mediawiki/2010/d/d7/Chiba_japanpage.jpg"></a><br />
</center><br />
</html><br />
|align="center"|[[Image:Chiba_pic.jpg]]<br />
|}<br />
<!--- The Mission, Experiments End ---><br />
<br />
<br />
<!--- Main Contents ---><br />
<html><br />
<body bgcolor="white" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<br />
</html><br />
<html><br />
<br><center><br />
<h3>Special Thanks!</h3><br />
<br><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/a/ac/Logo_geneart_tokyotech.gif" style="margin-right:20px"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/b/b5/Logo_mrgene_tokyotech.gif" height="45px"></a><br />
</html><br />
<!--- Main Contents End ---></div>
Lily.ch
http://2010.igem.org/Team:Chiba/test
Team:Chiba/test
2010-10-09T19:47:12Z
<p>Lily.ch: </p>
<hr />
<div><html><br />
<img src="https://static.igem.org/mediawiki/2010/2/2a/Chiba_home2.jpg" width="965" height="230"><br />
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<center><br />
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<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba">Home</a><br />
</li><br />
<br />
<li><a class="bannertoplinks" href="#">Team</a><br />
<ul style="z-index:1"><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Team">Team</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Member">Member</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/School">School</a></li><br />
</ul><br />
</li><br />
<li><a class="bannertoplinks" href="#">Project</a><br />
<ul style="z-index:1"><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Project">test1 Project</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Circuit_1">test2</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Circuit_2">test3</a></li><br />
</ul><br />
</li> <br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Parts">Parts</a><br />
</li><br />
<br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Modeling">Modeling</a><br />
</li><br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Notebook">Notebook</a><br />
</li><br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Safety">Safety</a><br />
</li><br />
<br />
<br />
</center> <br />
</div><br />
</html><br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| border="0" cellpadding="50" cellspacing="0" align="center"<br />
|-<br />
|<font size=5>PROJECT DESCRIPTION</font><br />
<br><br />
----<br />
<br />
<font size=3><br />
We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
</font><br><br />
----<br />
<br />
<html> <div style="text-align:center;"> <br />
<object type="application/x-shockwave-flash" data="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=ffcc66&bgcolor=006699&date_month=11&date_day=05&date_year=0&un=2010 iGEM JAMBOREE&size=normal&mo=11&da=05&yr=2010" width="350" height="100"><param name="movie" value="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=00B32B&bgcolor=003366&date_month=11&date_day=05&date_year=0&un=NEXT IGEM JAMBOREE&size=normal&mo=11&da=05&yr=2010" /><param name="bgcolor" value="#474747" /></object><img src="http://www.oneplusyou.com/q/img/bb_badges/countdown.jpg" alt="" style="display: none;" height="1" width="1" /><div><br />
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|[[Image:Chiba_ani_1.gif]]<br />
|}<br />
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<a href="http://chem.tf.chiba-u.jp/igem/"><img src="https://static.igem.org/mediawiki/2010/d/d7/Chiba_japanpage.jpg"></a><br />
</center><br />
</html><br />
|align="center"|[[Image:Chiba_pic.jpg]]<br />
|}<br />
<!--- The Mission, Experiments End ---><br />
<br />
<br />
<!--- Main Contents ---><br />
<html><br />
<body bgcolor="white" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<br />
</html><br />
<html><br />
<br><center><br />
<h3>Special Thanks!</h3><br />
<br><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/a/ac/Logo_geneart_tokyotech.gif" style="margin-right:20px"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/b/b5/Logo_mrgene_tokyotech.gif" height="45px"></a><br />
</html><br />
<!--- Main Contents End ---></div>
Lily.ch
http://2010.igem.org/Team:Chiba/test
Team:Chiba/test
2010-10-09T19:38:25Z
<p>Lily.ch: </p>
<hr />
<div><html><br />
<img src="https://static.igem.org/mediawiki/2010/2/2a/Chiba_home2.jpg" width="965" height="230"><br />
</html><br />
<html><br />
<p><br />
<style type="text/css"> <br />
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<br />
<!--- The Mission, Experiments ---><br />
<br />
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|-<br />
|<font size=5>PROJECT DESCRIPTION</font><br />
<br><br />
----<br />
<br />
<font size=3><br />
We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
</font><br><br />
----<br />
<br />
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<a href="http://chem.tf.chiba-u.jp/igem/"><img src="https://static.igem.org/mediawiki/2010/d/d7/Chiba_japanpage.jpg"></a><br />
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|align="center"|[[Image:Chiba_pic.jpg]]<br />
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<!--- The Mission, Experiments End ---><br />
<br />
<br />
<!--- Main Contents ---><br />
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<body bgcolor="white" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<br />
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<html><br />
<br><center><br />
<h3>Special Thanks!</h3><br />
<br><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/a/ac/Logo_geneart_tokyotech.gif" style="margin-right:20px"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/b/b5/Logo_mrgene_tokyotech.gif" height="45px"></a><br />
</html><br />
<!--- Main Contents End ---></div>
Lily.ch
http://2010.igem.org/Team:Chiba/test
Team:Chiba/test
2010-10-09T19:33:06Z
<p>Lily.ch: </p>
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<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| border="0" cellpadding="50" cellspacing="0" align="center"<br />
|-<br />
|<font size=5>PROJECT</font><br />
<br><br />
----<br />
<br />
<font size=3><br />
We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
</font><br><br />
----<br />
<br />
<html> <div style="text-align:center;"> <br />
<object type="application/x-shockwave-flash" data="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=ffcc66&bgcolor=006699&date_month=11&date_day=05&date_year=0&un=2010 iGEM JAMBOREE&size=normal&mo=11&da=05&yr=2010" width="350" height="100"><param name="movie" value="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=00B32B&bgcolor=003366&date_month=11&date_day=05&date_year=0&un=NEXT IGEM JAMBOREE&size=normal&mo=11&da=05&yr=2010" /><param name="bgcolor" value="#666666" /></object><img src="http://www.oneplusyou.com/q/img/bb_badges/countdown.jpg" alt="" style="display: none;" height="1" width="1" /><div><br />
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<a href="http://chem.tf.chiba-u.jp/igem/"><img src="https://static.igem.org/mediawiki/2010/d/d7/Chiba_japanpage.jpg"></a><br />
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</html><br />
|align="center"|[[Image:Chiba_pic.jpg]]<br />
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<!--- The Mission, Experiments End ---><br />
<br />
<br />
<!--- Main Contents ---><br />
<html><br />
<body bgcolor="white" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<br />
</html><br />
<html><br />
<br><center><br />
<h3>Special Thanks!</h3><br />
<br><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/a/ac/Logo_geneart_tokyotech.gif" style="margin-right:20px"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/b/b5/Logo_mrgene_tokyotech.gif" height="45px"></a><br />
</html><br />
<!--- Main Contents End ---></div>
Lily.ch
http://2010.igem.org/Team:Chiba/Modeling
Team:Chiba/Modeling
2010-10-09T18:16:05Z
<p>Lily.ch: </p>
<hr />
<div><!--- The Mission, Experiments ---><br />
<html><br />
<img src="https://static.igem.org/mediawiki/2010/2/2a/Chiba_home2.jpg" width="965" height="230"><br />
</html><br />
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<br />
<!--- The Mission, Experiments End ---><br />
<br />
<br />
<font size=2.5><br />
<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
Our second design of genetic double-click system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
In this system, we also use AHL input and GFP output.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
The transcription factors of output are T7 RNA Polymerase and cI repressor. <br><br />
<center>[[Image:Chiba_plan2_1.jpg]]</center><br><br><br />
<br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
At initial state, LuxR and cI protein are constitutively generated.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
cI binds to the operator site of PT7/cI.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
When 1st input is injected, LuxR-AHL dimmer binds to the Lux-box of the lux promoters so that T7 RNA Polymerase, cI434 and tetR protein are generated<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
at the same time.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
cI434 gradually accumulates, and gradually repress the transcription of T7 RNA Polymerase so that the expression of T7 RNA Polymerase can be shown<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
as a pulse.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
At the same time, Transcription of cI is stopped by tetR, cI decomposes and the PT7/cI promoter is unbound.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
This derepression occurs after the pulse of T7 RNAP has passed.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
In other words, the operator sites of PT7/cI is repressed by cI when there is pulse of T7 RNA Polymerase.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
So, it cannot transcribe GFP. TetR creates a time delay here from input to derepression.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
<center>[[Image:Chiba_planB_2.jpg]]</center><br><br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
Because of this time delay and one-time pulse, bacteria can never work by one input. <br><br />
<center>[[Image:Chiba_planB_3.jpg]]</center><br><br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
Before injecting 2nd input, we must create none-input-environment.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
So we choose to wash the 1st input. By washing , tetR protein and cI434 protein will degrade.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
cI434 protein should disappear so that when there is 2nd input, T7 RNA Polymerase will be shown as a pulse which is the same as the 1st time.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
cI will begin to generate if tetR protein gets lost.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
We recognize it as time-limit, when there is enough cI generated (this means cI repression is stronger than T7 RNA Polymerase activation),<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
there will be no GFP output.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
On the contrary, when there is less cI protein or no cI protein at the moment, T7 RNA Polymerase pulse can accumulate GFP output. <br><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
By the second injected AHL before the inhibition by cI, T7 RNA Polymerase binds to the PT7/cI promoter and transcribes the downstream GFP.</font><br><br />
<center>[[Image:Chiba_planB_4.jpg]]</center><br><br><br />
<br />
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|<font size=5>PROJECT</font><br />
<br />
----<br />
<br />
<font size=3><br />
We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
</font><br><br />
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|[[Image:Chiba_ani_1.gif]]<br />
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<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/a/ac/Logo_geneart_tokyotech.gif" style="margin-right:20px"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
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Lily.ch
http://2010.igem.org/Team:Chiba/test
Team:Chiba/test
2010-10-09T15:34:26Z
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<!--- The Mission, Experiments ---><br />
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<br />
----<br />
<br />
<font size=3><br />
We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
</font><br><br />
----<br />
|[[Image:Chiba_ani_1.gif]]<br />
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</body><br />
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</html><br />
<br><br />
<html><center><a href="https://twitter.com/Chiba_iGEM2010"><img src="https://static.igem.org/mediawiki/2010/8/80/Twitter-logo.jpg" width="150" height="95"></a><br><br><br />
<a href="http://chem.tf.chiba-u.jp/igem/"><img src="https://static.igem.org/mediawiki/2010/d/d7/Chiba_japanpage.jpg"></a><br />
</center><br />
</html><br />
|align="center"|[[Image:Chiba_pic.jpg]]<br />
|}<br />
<!--- The Mission, Experiments End ---><br />
<br />
<br />
<!--- Main Contents ---><br />
<html><br />
<body bgcolor="white" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<br />
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<html><br />
<br><center><br />
<h3>Special Thanks!</h3><br />
<br><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/a/ac/Logo_geneart_tokyotech.gif" style="margin-right:20px"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/b/b5/Logo_mrgene_tokyotech.gif" height="45px"></a><br />
</html><br />
<!--- Main Contents End ---></div>
Lily.ch
http://2010.igem.org/Team:Chiba/test
Team:Chiba/test
2010-10-09T15:13:37Z
<p>Lily.ch: </p>
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</html><br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| border="0" cellpadding="50" cellspacing="0" align="center"<br />
|-<br />
|<font size=5>Project title </font><font size=3>Genetic Double-Click System</font><br><br />
'''Abstract'''<br><br />
We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
|[[Image:Chiba_ani_1.gif]]<br />
|}<br />
{| border="0" cellpadding="50" cellspacing="0" align="center"<br />
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<br><br />
<html><center><a href="https://twitter.com/Chiba_iGEM2010"><img src="https://static.igem.org/mediawiki/2010/8/80/Twitter-logo.jpg" width="150" height="95"></a><br><br><br />
<a href="http://chem.tf.chiba-u.jp/igem/"><img src="https://static.igem.org/mediawiki/2010/d/d7/Chiba_japanpage.jpg"></a><br />
</center><br />
</html><br />
|align="center"|[[Image:Chiba_pic.jpg]]<br />
|}<br />
<!--- The Mission, Experiments End ---><br />
<br />
<br />
<!--- Main Contents ---><br />
<html><br />
<body bgcolor="white" text="black" link="blue" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<br />
</html><br />
<html><br />
<br><center><br />
<h3>Special Thanks!</h3><br />
<br><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/a/ac/Logo_geneart_tokyotech.gif" style="margin-right:20px"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/b/b5/Logo_mrgene_tokyotech.gif" height="45px"></a><br />
</html><br />
<!--- Main Contents End ---></div>
Lily.ch
http://2010.igem.org/Team:Chiba/test
Team:Chiba/test
2010-10-09T15:12:32Z
<p>Lily.ch: </p>
<hr />
<div><html><br />
<img src="https://static.igem.org/mediawiki/2010/2/2a/Chiba_home2.jpg" width="965" height="230"><br />
</html><br />
<html><br />
<p><br />
<style type="text/css"> <br />
<br />
#allcontent {<br />
width: 950px;<br />
}<br />
<br />
body {<br />
margin-left: auto;<br />
margin-right: auto;<br />
width: 1000px;<br />
}<br />
<br />
table {<br />
color: #33CC66;<br />
}<br />
<br />
#content {<br />
<br />
width: 980px;<br />
line-height: 1.2em;<br />
<br />
}<br />
<br />
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color: #000000;<br />
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<center><br />
<div id="headerlinks"><br />
<ul id="nav"><br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba">Home</a><br />
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<ul style="z-index:1"><br />
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<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Member">Member</a></li><br />
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</ul><br />
</li><br />
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<ul style="z-index:1"><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Project">test1 Project</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Circuit_1">test2</a></li><br />
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Chiba/Circuit_2">test3</a></li><br />
</ul><br />
</li> <br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Parts">Parts</a><br />
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<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Notebook">Notebook</a><br />
</li><br />
<li><br />
<a class="bannertoplinks" href="https://2010.igem.org/Team:Chiba/Safety">Safety</a><br />
</li><br />
<br />
<br />
</center> <br />
</div><br />
</html><br />
<br />
<br />
<!--- The Mission, Experiments ---><br />
<br />
{| border="0" cellpadding="50" cellspacing="0" align="center"<br />
|-<br />
|<font size=5>Project title </font><font size=3>Genetic Double-Click System</font><br><br />
'''Abstract'''<br><br />
We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
|[[Image:Chiba_ani_1.gif]]<br />
|}<br />
{| border="0" cellpadding="50" cellspacing="0" align="center"<br />
|-<br />
|<html><marquee><br />
<body bgcolor="#333366"><br />
<br />
<SCRIPT LANGUAGE="JavaScript"><br />
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function MakeArray(n){<br />
this.length=n;<br />
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}<br />
<br />
hex=new MakeArray(16);<br />
hex[11]="A"; hex[12]="B"; hex[13]="C"; hex[14]="D"; hex[15]="E"; hex[16]="F";<br />
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</html><br />
<br><br />
<html><center><a href="https://twitter.com/Chiba_iGEM2010"><img src="https://static.igem.org/mediawiki/2010/8/80/Twitter-logo.jpg" width="150" height="95"></a><br><br><br />
<a href="http://chem.tf.chiba-u.jp/igem/"><img src="https://static.igem.org/mediawiki/2010/d/d7/Chiba_japanpage.jpg"></a><br />
</center><br />
</html><br />
|align="center"|[[Image:Chiba_pic.jpg]]<br />
|}<br />
<!--- The Mission, Experiments End ---><br />
<br />
<br />
<!--- Main Contents ---><br />
<html><br />
<body bgcolor="white" text="black" link="blue" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<br />
</html><br />
<html><br />
<br><center><br />
<h3>Special Thanks!</h3><br />
<br><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/a/ac/Logo_geneart_tokyotech.gif" style="margin-right:20px"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/b/b5/Logo_mrgene_tokyotech.gif" height="45px"></a><br />
</html><br />
<!--- Main Contents End ---></div>
Lily.ch
http://2010.igem.org/Team:Chiba/Safety
Team:Chiba/Safety
2010-10-09T14:23:36Z
<p>Lily.ch: </p>
<hr />
<div><!--- The Mission, Experiments ---><br />
<html><br />
<img src="https://static.igem.org/mediawiki/2010/2/2a/Chiba_home2.jpg" width="965" height="230"><br />
</html><br />
{| style="color:#ffffff;background-color:#019fa0;" cellpadding="12" cellspacing="1" border="1" bordercolor="#cc0303" width="100%" align="center"<br />
|-<br />
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A:hover {color:#FF0000;text-decoration:none; }<br />
--> <br />
</style></html><br />
|align="center"|<html><a href=https://2010.igem.org/Team:Chiba/Team><font size=3.5>Team</font></a><style type="text/css"> <br />
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--> <br />
</style></html><br />
|align="center"|<html><a href=https://2010.igem.org/Team:Chiba/Project><font size=3.5>Project</font></a><style type="text/css"> <br />
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A:link {color:#F99343;text-decoration:none; }<br />
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A:hover {color:#FCFF00;text-decoration:none; }<br />
--> <br />
</style></html><br />
|align="center"|<html><a href=https://2010.igem.org/Team:Chiba/Parts><font size=3.5>Parts</font></a><style type="text/css"> <br />
<!-- <br />
A:link {color:#F99343;text-decoration:none; }<br />
A:visited {color:#F99343;text-decoration:none; }<br />
A:active {color:#F99343;text-decoration:none; }<br />
A:hover {color:#20B156;text-decoration:none; }<br />
--> <br />
</style></html><br />
|align="center"|<html><a href=https://2010.igem.org/Team:Chiba/Modeling><font size=3.5>Modeling</font></a><style type="text/css"> <br />
<!-- <br />
A:link {color:#F99343;text-decoration:none; }<br />
A:visited {color:#F99343;text-decoration:none; }<br />
A:active {color:#F99343;text-decoration:none; }<br />
A:hover {color:#4877FF;text-decoration:none; }<br />
--> <br />
</style></html><br />
|align="center"|<html><a href=https://2010.igem.org/Team:Chiba/Notebook><font size=3.5>Notebook</font></a><style type="text/css"> <br />
<!-- <br />
A:link {color:#F99343;text-decoration:none; }<br />
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A:hover {color:#4877FF;text-decoration:none; }<br />
--> <br />
</style></html><br />
|align="center"|<html><a href=https://2010.igem.org/Team:Chiba/Safety><font size=3.5>Safety</font></a><style type="text/css"> <br />
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A:link {color:#ffffff;text-decoration:none; }<br />
A:visited {color:#F99343;text-decoration:none; }<br />
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A:hover {color:#C21E9B;text-decoration:none; }<br />
--> <br />
</style></html><br />
|}<br />
<br />
<!--- The Mission, Experiments End ---><br />
<br />
<br />
<html><br />
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<br />
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<div id="layer1" style="width:200px; height:200px; position:absolute; left:20px; top:52px; z-index:1;"><br />
<p>&nbsp;</p><br />
</div><br />
<p>&nbsp;</p><br />
</body><br />
<br />
</html><br />
==Safety==<br />
<br />
Please use this page to answer the safety questions posed on the [[Safety | safety page]].<br />
<html><br />
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Lily.ch
http://2010.igem.org/Team:Chiba/Notebook_1
Team:Chiba/Notebook 1
2010-10-09T14:23:10Z
<p>Lily.ch: </p>
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<div><!--- The Mission, Experiments ---><br />
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<!--- The Mission, Experiments End ---><br />
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==Notebook==<br />
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8/31 We've started experiment. First, we researched the property of T7 promoter.<br><br />
9/5 We are researching promoter and inverters.<br><br />
10/7 We are on the bench!! Design primers, PCR and experimentsssssssssss :D</div>
Lily.ch