http://2010.igem.org/wiki/index.php?title=Special:Contributions/Andreshuerta&feed=atom&limit=50&target=Andreshuerta&year=&month=
2010.igem.org - User contributions [en]
2024-03-29T02:04:41Z
From 2010.igem.org
MediaWiki 1.16.5
http://2010.igem.org/Team:Tec-Monterrey/Geneticframe
Team:Tec-Monterrey/Geneticframe
2010-10-28T02:07:34Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/5/55/Geneticframe.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<ol class="menud"><br />
<a href="#development"><li>Development of the genetic frame for an “Intelligent Biosensor”<br />
</a></li><br />
<a href="#phage"><li>Phage Activators</a></li><br />
<ol class="menue"><br />
<a href="#proteins"><li>The Proteins</a></li><br />
<a href="#promoters"><li>The Promoters</a></li><br />
<a href="#sensitivity"><li>The Sensitivity Tuners</a></li><br />
<br />
<a href="#characterization"><li>Characterization</a></li><br />
<a href="#muc"><li>MuC sensitivity tuner (BBa_K427005)</a></li><br />
<a href="#mumor"><li>MuMor sensitivity tuner (BBa_K427006)</a></li><br />
<a href="#transfer"><li>Transfer Function Parameters</a></li><br />
</ol><br />
<a href="#references"><li>References</a></li><br />
<br />
</ol><br />
</p><br />
<br><br />
<h2><a name="development"></a> Development of the genetic frame for an “Intelligent Biosensor”</h2><br />
<p class="blah"><br />
After deciding we wanted to make a concentration sensitive biosensor, we began to investigate the different ways we could achieve our goal. After an initial research concerning biological reporters and the mechanisms currently used to make them, we began to read previous iGEM projects to look for useful BioBricks that could be applied to our work. Two past iGEM projects were of our particular interest: British Columbia’s 2009 project and Cambridge’s 2007 project. <br />
</p><br />
<br />
<p class="blah"><br />
<ul><br />
<li><b>British Columbia’s project</b><br />
<p class="blah"><br />
In the past iGEM competition The University of British Columbia team developed a project that resembled our initial proposition. They wanted to produce an <i>E. coli</i> that expressed different Fluorescent Proteins depending on the concentration of arabinose in the medium. Basically, they proposed a system that used three different sensitive promoters to activate a “Lock and Key” mechanism that enabled the expression of the Fluorescent Protein. There were 3 promoters: the wild type pBAD promoter that is sensitive to arabinose, and two others called pBAD weak and pBAD strong that were designed by the team via mutagenesis. As their name implies, the weak promoter is sensitive only to higher concentrations of arabinose and the strong promoter is sensitive to lower concentrations of arabinose compared to the wild type. Finally, to prevent the expression of all the fluorescent proteins when there was enough arabinose in the medium to activate all three promoters, they developed a “jammer” that consisted in placing a reverse promoter on the other side of the fluorescent protein. Thus, when both promoters were activated, the production of fluorescent protein ceased. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="https://2009.igem.org/Team:British_Columbia" target="blank">consult their wiki</a>)</i><br />
</p><br />
<br />
<li><b>Cambridge’s project</b><br />
<p class="blah"><br />
In 2007, the University of Cambridge iGEM team worked in developing both intracellular and intercellular communication pathways as well as a Gram-positive platform for synthetic biology. We were particularly interested in the intracellular project. It consisted of a PoPS amplifier made with transcriptional activators and promoters that were obtained from bacteriophage components. The Cambridge team decided to use activators from bacteriophage components because they are not common in the cellular signals of bacteria. In this particular case, they used activator and promoters from the bacteriophage PSP3 and fR73. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="http://parts.mit.edu/igem07/index.php/Cambridge" target="blank">consult their wiki</a>)</i><br />
</p><br />
</p><br />
</ul><br />
<p class="blah"><br />
After reading about these projects we decided that we could adapt our idea and build upon the achievements of those two previous teams. We wanted to contribute in a significant way, so we began by analyzing their results and identifying points that could use more work. We found that, in general, British Columbia’s proposal was complicated and apparently ineffective. The problem seemed to be in the “lock and key” mechanism, which didn’t seem to behave as expected. On the other hand, we found that their “jammers” could be very useful and they seemed to work as expected.<br />
</p><br />
<br />
<p class="blah"><br />
So we began to design of a different genetic frame to create “intelligent biosensors”, one that preferably didn’t contain a “lock and key” mechanism. Our solution is composed by a combination of parts of the two previously mentioned projects. We incorporated the “jammer” mechanism in addition to PoPS amplification systems in order to create a flexible genetic construction for concentration sensitive bio-reporters. Our proposed solution can be seen in Figure 1 (click on it to enlarge): <br />
</p><br />
<br><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/0/0c/Figure1tecp.png"><img src="https://static.igem.org/mediawiki/igem.org/0/0c/Figure1tecp.png" width="650" length="189"></a><br />
<b>Figure 1</b><br />
</center><br />
<br><br />
<p class="blah"><br />
In theory, this genetic construction is able to detect different concentrations of a compound provided that you have three different promoters that are sensitive to different concentrations of the substance. Based on the amount of compound detected, the bacteria will produce one of three reporter proteins. For our project, we decided to use the same sensors and reporter proteins that the University of British Columbia team used in their 2009 iGEM project. Therefore we began with 3 promoters: pBAD wild type, pBAD weak and pBAD strong; each promoter was able to detect arabinose at different concentrations. Our reporter proteins were GFP, YFP, and RFP (Cherry). We arranged the constructions so that GFP is produced when the cells were induced with low concentrations of arabinose, YFP is produced when induced with medium concentrations and RFP (Cherry) is produced when there is a high concentration. The mechanism that enables this behavior will be explained in two parts, the first part involves the detection of the compound and the second part concerns the production of the reporter protein. <br />
</p><br />
<p class="blah"><br />
The first part of the genetic construction is responsible for the detection of arabinose. This is achieved by using a series of promoters from the pBAD family that were developed and characterized by British Columbia. Each promoter is activated at a different concentration of arabinose, the strong promoter requires a higher concentration of arabinose than the weak promoter. When the promoter is activated, it enables the expression of a bacteriophage activator that works as a PoPS amplifier. The first amplifier we used was the one provided by the Cambrdige 2007 team. However, for our construction to work, we required two additional amplifiers. So we did some more research on bacteriophage activators and constructed two new PoPS amplifier systems in order to finish our construction. We consider these new amplifiers to be a valuable addition to the Registry of Standard Biological Parts, because they come from different bacteriophage families, this means that they produce different activator proteins and they can be used at the same time without causing interference. <br />
</p><br />
<p class="blah"><br />
Eventually we realized that if we didn’t have a mechanism that disabled the expression the PoPS amplifiers, when the cells were induced with high concentrations of arabinose, all three reporter proteins would be produced. Once again, incorporating the previous advances of the 2009 British Columbia Team, we were able to overcome this obstacle. The solution consisted in implementing their “jammer” systems, in order to suppress the expression of the PoPS amplifiers as the concentration of arabinose in the medium increases. The full mechanism is explained in the following figures: <br />
</p><br />
<ul><br />
<li>Figure 2: shows the sensor part of the final construct</li><br />
<br><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/2/2a/Figure2p.png"><img src="https://static.igem.org/mediawiki/igem.org/2/2a/Figure2p.png" width="650"></a><br />
<b>Figure 2</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 3: shows the sensor when there is a low concentration of arabinose</li><br />
<br><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/3/30/Figure3p.png"><img src="https://static.igem.org/mediawiki/igem.org/3/30/Figure3p.png" width="650"></a><br />
<b>Figure 3</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 4: shows the sensor when there is a medium concentration of arabinose</li><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/6/6b/Figure4p.png"><img src="https://static.igem.org/mediawiki/igem.org/6/6b/Figure4p.png" width="650"></a><br />
<b>Figure 4</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 5: shows the sensor when there is a high concentration of arabinose</li><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/1/13/Figure5p.png"><img src="https://static.igem.org/mediawiki/igem.org/1/13/Figure5p.png" width="650"></a><br />
<b>Figure 5</b><br />
</center><br><br />
<br />
<p class="blah"><br />
The second part of the construction is in charge of producing the reporter proteins. According to our research on biological sensors, the reporter molecule must be easy to detect and it must be a substance that is not naturally produced by the organism. Fluorescent proteins are great reporter proteins because fulfill both of these requirements. The team from British Columbia University used three different fluorescent proteins in their project in the 2009 competition: Green Fluorescent Protein (GFP), Yellow Fluorescent Protein (YFP), and the Cherry variety of the Red Fluorescent Protein (RFP Cherry). We ultimately decided to use the same three molecules because they have been widely characterized and there is plenty of information regarding their fluorescence spectrum and behavior. <br />
</p><br />
<br />
<p class="blah"><br />
When the sensing part of our construction produces one of the bacteriophage activators, this molecule enables the expression of one of the reporter proteins. Because these promoters are produce have very high PoPS numbers, the amount of reporter molecule produced is elevated, even though the arabinose concentration isn’t. The following figure is a detail that explains the mechanism behind the production of reporter proteins. <br />
</p><br />
<p class="blah"><br />
<ul><br />
<li>Figure 6: shows the reporter part of the final construct. </li><br />
</ul><br />
<br />
<center><br />
<a href="https://static.igem.org/mediawiki/2010/7/77/Figure6p.png"><img src="https://static.igem.org/mediawiki/2010/7/77/Figure6p.png" width="650"></a><br />
<b>Figure 6</b><br />
</center><br />
</p><br />
<br />
<br />
<br><br />
<br />
<h2><a name="phage"></a>Phage Activators</h2><br />
<p class="blah"><br />
The most basic bio-bricks created in our project were sequences form the genome of the Mu bacteriophage that attacks E. coli. We needed some sort of sensitivity tuner for our biosensor and the registry had only one family of promoters and protein activators that could be used for the construction of these devices. After some research we came to Mu bacteriophage, where we found two different families of promoters and protein activators: Mor protein – Pm promoter and C protein - Plys, Pi, Pp and Pmom promoters. We took the sequences reported sequences of Mor, Pm, C <a href="#kumaraswami">(Kumaraswami & Howe, 2004)</a> and Pmom <a href="#jiang">(Jiang, 2008)</a>. The proteins’ sequences were optimized for their expression in E. coli with Mr. Gene’s optimization algorithm; we added an Elowitzribosome binding site and finally the BioBrick prefix and suffix to create the bio-bricks that we used. We only added the prefix and suffix to the promoters’ sequences.<br />
</p><br />
<a name="proteins"></a><h4>The Proteins</h4><br />
<p class="blah"><br />
Mor and C are both proteins encoded in the genome of Mu bacteriophage, and they have similar characteristics. Both are proteins bind to DNA in a specific sequence, both have about the same size, sequence and have similar secondary structures and domains. Figure 7 <a href="#mathee">(Mathee, 1990)</a> shows the amino acid sequence alignment of Mor and C proteins.<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/81/Morandc.png"><br />
<b>Figure 7</b><br />
</center><br />
<br><br />
<p class="blah"><br />
In that image we can see the similarity between these two proteins, but we can also notice that the helix turn helix motifs are quite different, it is hypothesized that this domains are the ones that recognize and interact with the DNA sequence. According to <a href="#kumaraswami">Kumaraswami (2004)</a> and <a href="#jiang">Jiang (2008)</a> two domains can be observed on each of the proteins a Helix-Turn-Helix (HTH) domain and a dimerization domain, which suggests that the proteins can only activate their promoters when in dimers.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Mor (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427002">BBa_K427002</a>) and C (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427001">BBa_K427001</a>)<br />
</p><br />
<br />
<a name="promoters"></a><h4>The Promoters</h4><br />
<p class="blah"><br />
The first family of activators that we used was the Mor-Pm family. Pm promoter is the middle promoter of the Mu bacteriophage, where it initiates the transcription of C protein. The second family had four promoters: Plys, Pi, Pp and Pmom. We chose Pmom for our sensitivity tuners because out of the four promoters it is the most characterized in literature. It is normally activated by the C protein.<br />
</p><br />
<p class="blah"><br />
The activation of these promoters requires the holenzymeσ^70, which normally binds to the -35 and -10 consensus sequences, these promoters have the -10 but do not have the -35 sequences instead they have another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzymeσ^70 recognizes the promoter and transcription begins.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Pm (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427003">BBa_K427003</a>), Pmom (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427004">BBa_K427004</a>)<br />
</p><br />
<br />
<a name="sensitivity"></a> <h4>The Sensitivity Tuners</h4><br />
<p class="blah"><br />
These devices were designed with two purposes in mind, increase the PoPS output of the pBad promoter (any promoter would work) and change its almost linear production into an almost Boolean one. These parts have the following structure: activator protein, transcriptional stop and phage promoter. The sensitivity tuners can be used to achieve both purposes on any construction they just have to be inserted between the desired promoter and the desired coding sequence.<br />
</p><br />
<p class="blah"><br />
In our project we used three sensitivity tuners, one made by Cambridge in previous iGEMcompetitions;this one is built with phiR73 delta activator (<a href="http://partsregistry.org/Part:BBa_I746352">BBa_I746352</a>), a bidirectional transcriptional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>) and Po phage promoter (<a href="BBa_I746361">BBa_I746361</a>). The other two were built by us using the Mor and C families. They are MuC sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005">BBa_K427005</a>) and MuMor sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006">BBa_K427006</a>). The first one includes MuC protein activator (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427002">BBa_K427002</a>) bidirectional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>) and Pmom promoter (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427004">BBA_K427004</a>). The other includes MuMor protein activator (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427001">BBa_K427001</a>) bidirectional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>) and Pmom promoter (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427003">BBA_K427003</a>).<br />
</p><br />
<br />
<ul class="lista"><br />
<p class="blah"><br />
<li>Figure 8: MuC sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005">BBa_K427005</a>)</li><br />
</ul><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/49/Figure8p.png"><br />
<b>Figure 8</b><br />
</center><br />
</p><br />
<br />
<br />
<ul class="lista"><br />
<p class="blah"><br />
<li>Figure 9: MuMor sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006">BBa_K427006</a>)</li><br />
</ul><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/e/eb/Figure9p.png"><br />
<b>Figure 9</b><br />
</center><br />
</p><br />
<br><br />
<a name="characterization"></a> <h4>Characterization</h4><br />
<p class="blah"><br />
We characterized both sensitivity tuners using Hill equations:</p><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/4d/Hilleq.png"><br />
</center><br />
<p class="blah"><br />
In order to do this we had to build another two parts, they were used only to characterize the sensitivity tuners they have a bidirectional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>), PBad Weak (<a href="http://partsregistry.org/Part:BBa_K206001">BBa_K206001</a>) the sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005">BBa_K427005</a> or <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006">BBa_K427006</a>) and GFP reporter (<a href="http://partsregistry.org/Part:BBa_E0840:Experience">BBa_E0840</a>). These parts are <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427007">BBa_K427007</a> for the MuC sensitivity tuner and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427008">BBa_K427008</a> for the MuMor sensitivity tuner. <br />
</p><br />
<p class="blah"><br />
All the characterizations were made in the BW27783 strain of E. coli. This strain was modified to internalize arabinose so that the concentration inside the cell is the same as the concentration outside. Another modification is that BW27783 does not use or degrade arabinose.The graphs show the data used to obtain the coefficients reported in the table. The protocol we used is described in the Protocol section of our wiki.<br />
</p><br />
<br />
<a name="muc"></a> <h4> MuC sensitivity tuner (BBa_K427005)</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/a/a6/Mucgraph.png"><br />
</center><br />
<br><br />
<a name="mumor"></a> <h4>MuMor sensitivity tuner (BBa_K427006)</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0f/Mumorgraph.png"><br />
</center><br />
<br><br />
<a name="transfer"></a> <h4>Transfer function parameters</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/7/72/Transferfunction.png"><br />
</center><br />
<br><br><br />
<br />
<a name="references"></a> <h2>References</h2><br />
<a name="jiang"></a><b> Jiang, Y. a. (2008).</b> Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.<br />
<br><br><br />
<p class="blah"><a name="kumaraswami"></a><b> Kumaraswami, M., & Howe, M. a.-W. (2004).</b> Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.<br />
<br><br><br />
<a name="mathee"></a><b> Mathee, K. a. (1990).</b> Identification of a Positive Regulator of the Mu Middle Operon. Journal of bacteriology, 6641-6650.<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Geneticframe
Team:Tec-Monterrey/Geneticframe
2010-10-28T02:07:20Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/5/55/Geneticframe.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<ol class="menud"><br />
<a href="#development"><li>Development of the genetic frame for an “Intelligent Biosensor”<br />
</a></li><br />
<a href="#phage"><li>Phage Activators</a></li><br />
<ol class="menue"><br />
<a href="#proteins"><li>The Proteins</a></li><br />
<a href="#promoters"><li>The Promoters</a></li><br />
<a href="#sensitivity"><li>The Sensitivity Tuners</a></li><br />
<br />
<a href="#characterization"><li>Characterization</a></li><br />
<a href="#muc"><li>MuC sensitivity tuner (BBa_K427005)</a></li><br />
<a href="#mumor"><li>MuMor sensitivity tuner (BBa_K427006)</a></li><br />
<a href="#transfer"><li>Transfer Function Parameters</a></li><br />
</ol><br />
<a href="#references"><li>References</a></li><br />
<br />
</ol><br />
</p><br />
<br><br />
<h2><a name="development"></a> Development of the genetic frame for an “Intelligent Biosensor”</h2><br />
<p class="blah"><br />
After deciding we wanted to make a concentration sensitive biosensor, we began to investigate the different ways we could achieve our goal. After an initial research concerning biological reporters and the mechanisms currently used to make them, we began to read previous iGEM projects to look for useful BioBricks that could be applied to our work. Two past iGEM projects were of our particular interest: British Columbia’s 2009 project and Cambridge’s 2007 project. <br />
</p><br />
<br />
<p class="blah"><br />
<ul><br />
<li><b>British Columbia’s project</b><br />
<p class="blah"><br />
In the past iGEM competition The University of British Columbia team developed a project that resembled our initial proposition. They wanted to produce an <i>E. coli</i> that expressed different Fluorescent Proteins depending on the concentration of arabinose in the medium. Basically, they proposed a system that used three different sensitive promoters to activate a “Lock and Key” mechanism that enabled the expression of the Fluorescent Protein. There were 3 promoters: the wild type pBAD promoter that is sensitive to arabinose, and two others called pBAD weak and pBAD strong that were designed by the team via mutagenesis. As their name implies, the weak promoter is sensitive only to higher concentrations of arabinose and the strong promoter is sensitive to lower concentrations of arabinose compared to the wild type. Finally, to prevent the expression of all the fluorescent proteins when there was enough arabinose in the medium to activate all three promoters, they developed a “jammer” that consisted in placing a reverse promoter on the other side of the fluorescent protein. Thus, when both promoters were activated, the production of fluorescent protein ceased. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="https://2009.igem.org/Team:British_Columbia" target="blank">consult their wiki</a>)</i><br />
</p><br />
<br />
<li><b>Cambridge’s project</b><br />
<p class="blah"><br />
In 2007, the University of Cambridge iGEM team worked in developing both intracellular and intercellular communication pathways as well as a Gram-positive platform for synthetic biology. We were particularly interested in the intracellular project. It consisted of a PoPS amplifier made with transcriptional activators and promoters that were obtained from bacteriophage components. The Cambridge team decided to use activators from bacteriophage components because they are not common in the cellular signals of bacteria. In this particular case, they used activator and promoters from the bacteriophage PSP3 and fR73. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="http://parts.mit.edu/igem07/index.php/Cambridge" target="blank">consult their wiki</a>)</i><br />
</p><br />
</p><br />
</ul><br />
<p class="blah"><br />
After reading about these projects we decided that we could adapt our idea and build upon the achievements of those two previous teams. We wanted to contribute in a significant way, so we began by analyzing their results and identifying points that could use more work. We found that, in general, British Columbia’s proposal was complicated and apparently ineffective. The problem seemed to be in the “lock and key” mechanism, which didn’t seem to behave as expected. On the other hand, we found that their “jammers” could be very useful and they seemed to work as expected.<br />
</p><br />
<br />
<p class="blah"><br />
So we began to design of a different genetic frame to create “intelligent biosensors”, one that preferably didn’t contain a “lock and key” mechanism. Our solution is composed by a combination of parts of the two previously mentioned projects. We incorporated the “jammer” mechanism in addition to PoPS amplification systems in order to create a flexible genetic construction for concentration sensitive bio-reporters. Our proposed solution can be seen in Figure 1 (click on it to enlarge): <br />
</p><br />
<br><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/0/0c/Figure1tecp.png"><img src="https://static.igem.org/mediawiki/igem.org/0/0c/Figure1tecp.png" width="650" length="189"></a><br />
<b>Figure 1</b><br />
</center><br />
<br><br />
<p class="blah"><br />
In theory, this genetic construction is able to detect different concentrations of a compound provided that you have three different promoters that are sensitive to different concentrations of the substance. Based on the amount of compound detected, the bacteria will produce one of three reporter proteins. For our project, we decided to use the same sensors and reporter proteins that the University of British Columbia team used in their 2009 iGEM project. Therefore we began with 3 promoters: pBAD wild type, pBAD weak and pBAD strong; each promoter was able to detect arabinose at different concentrations. Our reporter proteins were GFP, YFP, and RFP (Cherry). We arranged the constructions so that GFP is produced when the cells were induced with low concentrations of arabinose, YFP is produced when induced with medium concentrations and RFP (Cherry) is produced when there is a high concentration. The mechanism that enables this behavior will be explained in two parts, the first part involves the detection of the compound and the second part concerns the production of the reporter protein. <br />
</p><br />
<p class="blah"><br />
The first part of the genetic construction is responsible for the detection of arabinose. This is achieved by using a series of promoters from the pBAD family that were developed and characterized by British Columbia. Each promoter is activated at a different concentration of arabinose, the strong promoter requires a higher concentration of arabinose than the weak promoter. When the promoter is activated, it enables the expression of a bacteriophage activator that works as a PoPS amplifier. The first amplifier we used was the one provided by the Cambrdige 2007 team. However, for our construction to work, we required two additional amplifiers. So we did some more research on bacteriophage activators and constructed two new PoPS amplifier systems in order to finish our construction. We consider these new amplifiers to be a valuable addition to the Registry of Standard Biological Parts, because they come from different bacteriophage families, this means that they produce different activator proteins and they can be used at the same time without causing interference. <br />
</p><br />
<p class="blah"><br />
Eventually we realized that if we didn’t have a mechanism that disabled the expression the PoPS amplifiers, when the cells were induced with high concentrations of arabinose, all three reporter proteins would be produced. Once again, incorporating the previous advances of the 2009 British Columbia Team, we were able to overcome this obstacle. The solution consisted in implementing their “jammer” systems, in order to suppress the expression of the PoPS amplifiers as the concentration of arabinose in the medium increases. The full mechanism is explained in the following figures: <br />
</p><br />
<ul><br />
<li>Figure 2: shows the sensor part of the final construct</li><br />
<br><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/2/2a/Figure2p.png"><img src="https://static.igem.org/mediawiki/igem.org/2/2a/Figure2p.png" width="650"></a><br />
<b>Figure 2</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 3: shows the sensor when there is a low concentration of arabinose</li><br />
<br><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/3/30/Figure3p.png"><img src="https://static.igem.org/mediawiki/igem.org/3/30/Figure3p.png" width="650"></a><br />
<b>Figure 3</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 4: shows the sensor when there is a medium concentration of arabinose</li><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/6/6b/Figure4p.png"><img src="https://static.igem.org/mediawiki/igem.org/6/6b/Figure4p.png" width="650"></a><br />
<b>Figure 4</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 5: shows the sensor when there is a high concentration of arabinose</li><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/1/13/Figure5p.png"><img src="https://static.igem.org/mediawiki/igem.org/1/13/Figure5p.png" width="650"></a><br />
<b>Figure 5</b><br />
</center><br><br />
<br />
<p class="blah"><br />
The second part of the construction is in charge of producing the reporter proteins. According to our research on biological sensors, the reporter molecule must be easy to detect and it must be a substance that is not naturally produced by the organism. Fluorescent proteins are great reporter proteins because fulfill both of these requirements. The team from British Columbia University used three different fluorescent proteins in their project in the 2009 competition: Green Fluorescent Protein (GFP), Yellow Fluorescent Protein (YFP), and the Cherry variety of the Red Fluorescent Protein (RFP Cherry). We ultimately decided to use the same three molecules because they have been widely characterized and there is plenty of information regarding their fluorescence spectrum and behavior. <br />
</p><br />
<br />
<p class="blah"><br />
When the sensing part of our construction produces one of the bacteriophage activators, this molecule enables the expression of one of the reporter proteins. Because these promoters are produce have very high PoPS numbers, the amount of reporter molecule produced is elevated, even though the arabinose concentration isn’t. The following figure is a detail that explains the mechanism behind the production of reporter proteins. <br />
</p><br />
<p class="blah"><br />
<ul><br />
<li>Figure 6: shows the reporter part of the final construct. </li><br />
</ul><br />
<br />
<center><br />
<a href="https://static.igem.org/mediawiki/2010/7/77/Figure6p.png"><img src="https://static.igem.org/mediawiki/igem.org/7/77/Figure6p.png" width="650"></a><br />
<b>Figure 6</b><br />
</center><br />
</p><br />
<br />
<br />
<br><br />
<br />
<h2><a name="phage"></a>Phage Activators</h2><br />
<p class="blah"><br />
The most basic bio-bricks created in our project were sequences form the genome of the Mu bacteriophage that attacks E. coli. We needed some sort of sensitivity tuner for our biosensor and the registry had only one family of promoters and protein activators that could be used for the construction of these devices. After some research we came to Mu bacteriophage, where we found two different families of promoters and protein activators: Mor protein – Pm promoter and C protein - Plys, Pi, Pp and Pmom promoters. We took the sequences reported sequences of Mor, Pm, C <a href="#kumaraswami">(Kumaraswami & Howe, 2004)</a> and Pmom <a href="#jiang">(Jiang, 2008)</a>. The proteins’ sequences were optimized for their expression in E. coli with Mr. Gene’s optimization algorithm; we added an Elowitzribosome binding site and finally the BioBrick prefix and suffix to create the bio-bricks that we used. We only added the prefix and suffix to the promoters’ sequences.<br />
</p><br />
<a name="proteins"></a><h4>The Proteins</h4><br />
<p class="blah"><br />
Mor and C are both proteins encoded in the genome of Mu bacteriophage, and they have similar characteristics. Both are proteins bind to DNA in a specific sequence, both have about the same size, sequence and have similar secondary structures and domains. Figure 7 <a href="#mathee">(Mathee, 1990)</a> shows the amino acid sequence alignment of Mor and C proteins.<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/81/Morandc.png"><br />
<b>Figure 7</b><br />
</center><br />
<br><br />
<p class="blah"><br />
In that image we can see the similarity between these two proteins, but we can also notice that the helix turn helix motifs are quite different, it is hypothesized that this domains are the ones that recognize and interact with the DNA sequence. According to <a href="#kumaraswami">Kumaraswami (2004)</a> and <a href="#jiang">Jiang (2008)</a> two domains can be observed on each of the proteins a Helix-Turn-Helix (HTH) domain and a dimerization domain, which suggests that the proteins can only activate their promoters when in dimers.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Mor (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427002">BBa_K427002</a>) and C (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427001">BBa_K427001</a>)<br />
</p><br />
<br />
<a name="promoters"></a><h4>The Promoters</h4><br />
<p class="blah"><br />
The first family of activators that we used was the Mor-Pm family. Pm promoter is the middle promoter of the Mu bacteriophage, where it initiates the transcription of C protein. The second family had four promoters: Plys, Pi, Pp and Pmom. We chose Pmom for our sensitivity tuners because out of the four promoters it is the most characterized in literature. It is normally activated by the C protein.<br />
</p><br />
<p class="blah"><br />
The activation of these promoters requires the holenzymeσ^70, which normally binds to the -35 and -10 consensus sequences, these promoters have the -10 but do not have the -35 sequences instead they have another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzymeσ^70 recognizes the promoter and transcription begins.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Pm (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427003">BBa_K427003</a>), Pmom (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427004">BBa_K427004</a>)<br />
</p><br />
<br />
<a name="sensitivity"></a> <h4>The Sensitivity Tuners</h4><br />
<p class="blah"><br />
These devices were designed with two purposes in mind, increase the PoPS output of the pBad promoter (any promoter would work) and change its almost linear production into an almost Boolean one. These parts have the following structure: activator protein, transcriptional stop and phage promoter. The sensitivity tuners can be used to achieve both purposes on any construction they just have to be inserted between the desired promoter and the desired coding sequence.<br />
</p><br />
<p class="blah"><br />
In our project we used three sensitivity tuners, one made by Cambridge in previous iGEMcompetitions;this one is built with phiR73 delta activator (<a href="http://partsregistry.org/Part:BBa_I746352">BBa_I746352</a>), a bidirectional transcriptional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>) and Po phage promoter (<a href="BBa_I746361">BBa_I746361</a>). The other two were built by us using the Mor and C families. They are MuC sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005">BBa_K427005</a>) and MuMor sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006">BBa_K427006</a>). The first one includes MuC protein activator (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427002">BBa_K427002</a>) bidirectional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>) and Pmom promoter (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427004">BBA_K427004</a>). The other includes MuMor protein activator (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427001">BBa_K427001</a>) bidirectional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>) and Pmom promoter (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427003">BBA_K427003</a>).<br />
</p><br />
<br />
<ul class="lista"><br />
<p class="blah"><br />
<li>Figure 8: MuC sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005">BBa_K427005</a>)</li><br />
</ul><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/49/Figure8p.png"><br />
<b>Figure 8</b><br />
</center><br />
</p><br />
<br />
<br />
<ul class="lista"><br />
<p class="blah"><br />
<li>Figure 9: MuMor sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006">BBa_K427006</a>)</li><br />
</ul><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/e/eb/Figure9p.png"><br />
<b>Figure 9</b><br />
</center><br />
</p><br />
<br><br />
<a name="characterization"></a> <h4>Characterization</h4><br />
<p class="blah"><br />
We characterized both sensitivity tuners using Hill equations:</p><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/4d/Hilleq.png"><br />
</center><br />
<p class="blah"><br />
In order to do this we had to build another two parts, they were used only to characterize the sensitivity tuners they have a bidirectional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>), PBad Weak (<a href="http://partsregistry.org/Part:BBa_K206001">BBa_K206001</a>) the sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005">BBa_K427005</a> or <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006">BBa_K427006</a>) and GFP reporter (<a href="http://partsregistry.org/Part:BBa_E0840:Experience">BBa_E0840</a>). These parts are <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427007">BBa_K427007</a> for the MuC sensitivity tuner and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427008">BBa_K427008</a> for the MuMor sensitivity tuner. <br />
</p><br />
<p class="blah"><br />
All the characterizations were made in the BW27783 strain of E. coli. This strain was modified to internalize arabinose so that the concentration inside the cell is the same as the concentration outside. Another modification is that BW27783 does not use or degrade arabinose.The graphs show the data used to obtain the coefficients reported in the table. The protocol we used is described in the Protocol section of our wiki.<br />
</p><br />
<br />
<a name="muc"></a> <h4> MuC sensitivity tuner (BBa_K427005)</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/a/a6/Mucgraph.png"><br />
</center><br />
<br><br />
<a name="mumor"></a> <h4>MuMor sensitivity tuner (BBa_K427006)</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0f/Mumorgraph.png"><br />
</center><br />
<br><br />
<a name="transfer"></a> <h4>Transfer function parameters</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/7/72/Transferfunction.png"><br />
</center><br />
<br><br><br />
<br />
<a name="references"></a> <h2>References</h2><br />
<a name="jiang"></a><b> Jiang, Y. a. (2008).</b> Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.<br />
<br><br><br />
<p class="blah"><a name="kumaraswami"></a><b> Kumaraswami, M., & Howe, M. a.-W. (2004).</b> Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.<br />
<br><br><br />
<a name="mathee"></a><b> Mathee, K. a. (1990).</b> Identification of a Positive Regulator of the Mu Middle Operon. Journal of bacteriology, 6641-6650.<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/File:Figure6p.png
File:Figure6p.png
2010-10-28T02:06:00Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Journal
Team:Tec-Monterrey/Journal
2010-10-28T01:57:54Z
<p>Andreshuerta: New page: <html> <head> <meta http-equiv="content-type" content="text/html;charset=utf-8" /> <title>Tec de Monterrey </title> <style> #search-controls { display: none; } </style> </head> ...</p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<style type="text/css"><br />
center img {border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f5/Journaltitulo.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
</p> <br />
<div class="footercon"><br />
<p class="blahimgizq"><br />
All of our experiments and activities performed during the summer were recorded in one journal, quite a big journal, actually. If you wish to see this document, you can download it by clicking the next image:<br />
</p><br />
<br><br />
<center><br />
<a href="https://2010.igem.org/Image:Journal_Tec_Monterrey.pdf" target="blank"><img src="https://static.igem.org/mediawiki/2010/c/c7/Bitacora_cora_cora.jpg"></a><br />
</center><br />
<br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<br />
</html></div>
Andreshuerta
http://2010.igem.org/File:Journal_Tec_Monterrey.pdf
File:Journal Tec Monterrey.pdf
2010-10-28T01:43:32Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/File:Journaltitulo.png
File:Journaltitulo.png
2010-10-28T00:39:19Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Project
Team:Tec-Monterrey/Project
2010-10-28T00:33:33Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/9/92/Project.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
In this section you will find all the information related to our iGEM Project, how we came up with the idea, the theory behind our experiments and the results and parts that were obtained after our hard work.<br />
<br />
<ol class="menud"><br />
<a href="#abstract"><li>Project Abstract</a></li><br />
<a href="#overview"><li>Project Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a>: To know more about the basis of synthetic biology and bacterial reporters.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a>: The core of our project and our main proposal.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a>: BioBricks we developed during the project.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a>: Our considerations on what will be the next steps to follow and the applications that our project holds for the future.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/8/8f/Projectbelow.jpg"><br />
</center><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<a name="abstract"></a><img src="https://static.igem.org/mediawiki/2010/7/7e/Abstracttitulo.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. The first bacterial reporters appeared 20 years ago, although these early tests didn't use genetically modified microorganisms. Further research and development in the area of genetic engineering has resulted in newer and more sophisticated bacterial sensors, capable of detecting the presence of contaminants, sugars and amino acids in different media such as soil and water. However, most bacterial sensors can only detect the presence of a compound at a certain concentration and currently there are few documented bacterial sensors that can detect and report different concentrations of the compound of interest. Our objective was to develop a genetic frame, compatible with the BioBrick standard, for the creation of a concentration-sensitive bacterial sensor. In the process we also developed and characterized BioBricks for two new families of phage activators.<br />
<br />
<br />
<br />
<br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<div class="hd"><div class="g"> </div></div><br />
<br />
<div class="top"><br />
<a name="overview"></a><img src="https://static.igem.org/mediawiki/2010/e/ef/Projectoverview.png" border="0" ><br />
<div class="bdy"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/7/73/Semaforo.jpg"><br />
<h2>Development of a genetic frame for the creation of concentration-sensitive bacterial sensors</h2><br />
</p><br />
<p class="blahimgder"><br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensor, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a><br />
</p><p class="blah"><br />
The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration. <br />
</p><p class="blah"><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
</p><p class="blah"><br />
With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.<br />
</p><br />
<br />
<h2>References</h2><br />
<p class="blah"><a name="jaeger"></a><b>Jaeger, C. H., et. Al. (June 1999)</b> Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690<br />
<br><br><br />
<a name="van"></a><b>Van der Meer, J. R. and Belkin, S. (July 2010)</b> Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522<br />
<br><br />
<br><br />
<a name="willardson"></a><b>Willardson, B. M., et. Al. (March 1998)</b> Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012<br />
<br><br><br />
<a name="yagi"></a><b>Yagi, K. (2007)</b> Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258<br />
<br><br></p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/File:Projectbelow.jpg
File:Projectbelow.jpg
2010-10-28T00:28:58Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey_/Sponsors
Team:Tec-Monterrey /Sponsors
2010-10-28T00:25:16Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/5/56/Sponsorstit.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
We would really like to thank our sponsors for their enormous help in making this project a reality. Without their generous support, we wouldn’t have been able to achieve our goals for this project. And to everyone who reads this page, we would like to tell you that all these enterprises provide excellent services and we sincerely recommend their products to anyone interested. For more information you can visit their webpage or contact the team. <br />
<br />
<ol class="menud"><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a>: people we couldn’t have managed without.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/a/a5/Sponsorsbelow.jpg"><br />
</center><br />
<br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/File:Sponsorsbelow.jpg
File:Sponsorsbelow.jpg
2010-10-28T00:23:41Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Team
Team:Tec-Monterrey/Team
2010-10-28T00:21:10Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/f/f4/Teamtit.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
Here you’ll get to know who was part of the team, whether as an undergrad, an instructor or an advisor. Also, you’ll get to know the role that every member of the team played in the development of the project. Finally, you'll find information about our headquarters, the lab, and our university.<br />
<br />
<ol class="menud"><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a>: The ten students that took part in this journey.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a>: Our guides in the path of synthetic biology.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a>: The role each person played in the team.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a>: The places that became our home: the lab, the Biotechnology Center and our university.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/1/12/Teambelow.jpg"><br />
</center><br />
<br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/File:Teambelow.jpg
File:Teambelow.jpg
2010-10-28T00:20:07Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Bonus
Team:Tec-Monterrey/Bonus
2010-10-27T23:58:40Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/1/1f/Bonus.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
A more relaxed section to place our photos and the information on our beloved mascot: Wiki Wiki. Here you’ll get how we got along, what we did when we were together and how we became increasingly random as the time passed.<br />
<br />
<ol class="menud"><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a>: photos from the lab and elsewhere.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a>: everything you need to know about Wiki Wiki, our peacock.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/b/bd/Bonusbelow.jpg"><br />
</center><br />
<br />
<br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/File:Bonusbelow.jpg
File:Bonusbelow.jpg
2010-10-27T23:57:07Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Documentation
Team:Tec-Monterrey/Documentation
2010-10-27T23:56:07Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/d/d1/Documentation.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
This is the section in which we registered all we did during the summer: our laboratory journal, the protocols we followed, our considerations on safety & ethics and our contribution to human practices, which was one of our major objectives after encountering some setbacks in the development of our project. Hopefully, the information contained in this section will prove to be very useful for upcoming iGEM teams.<br />
<br />
<ol class="menud"><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a>: All our lab activities, the experiments we carried out to get to our results.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a>: The rules we follow when experimenting.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a>: The team considerations on safety and ethics concerning biological research.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a>: Our contribution for the improvement of research conditions in our country.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/1/10/Documentationbelow.png"><br />
</center><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Documentation
Team:Tec-Monterrey/Documentation
2010-10-27T23:53:58Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/d/d1/Documentation.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
This is the section in which we registered all we did during the summer: our laboratory journal, the protocols we followed, our considerations on safety & ethics and our contribution to human practices, which was one of our major objectives after encountering some setbacks in the development of our project. Hopefully, the information contained in this section will prove to be very useful for upcoming iGEM teams.<br />
<br />
<ol class="menud"><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a>: All our lab activities, the experiments we carried out to get to our results.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a>: The rules we follow when experimenting.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a>: The team considerations on safety and ethics concerning biological research.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a>: Our contribution for the improvement of research conditions in our country.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/1/10/Documentationbelow.png"><br />
</center><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/File:Documentationbelow.png
File:Documentationbelow.png
2010-10-27T23:52:50Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey
Team:Tec-Monterrey
2010-10-27T23:48:25Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
color:white;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<style><br />
<br />
<br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<div style="text-align:justify"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/a/a1/Itesmlogo.jpg"><br />
Welcome to the iGEM Tec-Monterrey Team’s Wiki. This is the first time that our university participates in iGEM and we hope that you find our project interesting and enjoy it as much as we enjoyed working on it. Feel free to navigate our wiki and become part of this unforgettable experience.<br />
</p><br />
</div><br />
</div><br />
<div class="ft"><div class="c"></div></div> <br />
<div class="bgr"><br><br />
</div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/9/92/Project.png" border="0" ><br />
<div class="bdy"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/7/73/Semaforo.jpg" width="80"><br />
<br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. The first bacterial reporters appeared 20 years ago, although these early tests didn't use genetically modified microorganisms. Further research and development in the area of genetic engineering has resulted in newer and more sophisticated bacterial sensors, capable of detecting the presence of contaminants, sugars and amino acids in different media such as soil and water. However, most bacterial sensors can only detect the presence of a compound at a certain concentration and currently there are few documented bacterial sensors that can detect and report different concentrations of the compound of interest. Our objective was to develop a genetic frame, compatible with the BioBrick standard, for the creation of a concentration-sensitive bacterial sensor. In the process we also developed and characterized BioBricks for two new families of phage activators. <a href="https://2010.igem.org/Team:Tec-Monterrey/Project"><b>Read More</b></a><br />
<br />
<br />
<br />
<br />
</p><br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div><br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="headercon"><br />
<img src="https://static.igem.org/mediawiki/2010/8/8e/Titulos3.png"><br />
</div><br />
<div class="leftcon"><p class="blah"><a href="https://2010.igem.org/Team:Tec-Monterrey/Team"><img src="https://static.igem.org/mediawiki/2010/1/13/Teamsmall.png"></a></p> <p class="blah">The Tec-Monterrey iGEM team is made up of ten 7th semester Biotechnology Engineering students from the Tecnológico de Monterrey in Monterrey, Mexico...<a href="https://2010.igem.org/Team:Tec-Monterrey/Team"><b>Read more</b></a></p></div><br />
<div class="rightcon"><p class="blah"><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation"><img src=https://static.igem.org/mediawiki/2010/4/4e/Documsmall.png></a></p><p class="blah">The registry of everything we did in the summer, our lab journal, the protocols, our contribution to human practices… <a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation"><b>Read more</b></a></p></div><br />
<div class="rightcon"><p class="blah"><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus"><img src="https://static.igem.org/mediawiki/2010/e/e3/Bonussmall.png"></a></p><p class="blah">Photos from our summer work and information on our beloved Wiki Wiki… <a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus"><b>Read more</b></a><br />
</p></div><br />
<div class="footercon"></div><br />
<br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<div class="bgr"><br><br />
</div><br />
</html>\</div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey
Team:Tec-Monterrey
2010-10-27T23:46:10Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
color:white;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<style><br />
<br />
<br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<div style="text-align:justify"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/a/a1/Itesmlogo.jpg"><br />
Welcome to the iGEM Tec-Monterrey Team’s Wiki. This is the first time that our university participates in iGEM and we hope that you find our project interesting and enjoy it as much as we enjoyed working on it. Feel free to navigate our wiki and become part of this unforgettable experience.<br />
</p><br />
</div><br />
</div><br />
<div class="ft"><div class="c"></div></div> <br />
<div class="bgr"><br><br />
</div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/9/92/Project.png" border="0" ><br />
<div class="bdy"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/7/73/Semaforo.jpg" width="80"><br />
<br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. The first bacterial reporters appeared 20 years ago, although these early tests didn't use genetically modified microorganisms. Further research and development in the area of genetic engineering has resulted in newer and more sophisticated bacterial sensors, capable of detecting the presence of contaminants, sugars and amino acids in different media such as soil and water. However, most bacterial sensors can only detect the presence of a compound at a certain concentration and currently there are few documented bacterial sensors that can detect and report different concentrations of the compound of interest. Our objective was to develop a genetic frame, compatible with the BioBrick standard, for the creation of a concentration-sensitive bacterial sensor. In the process we also developed and characterized BioBricks for two new families of phage activators. <a href="https://2010.igem.org/Team:Tec-Monterrey/Project"><b>Read More</b></a><br />
<br />
<br />
<br />
<br />
</p><br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div><br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="headercon"><br />
<img src="https://static.igem.org/mediawiki/2010/8/8e/Titulos3.png"><br />
</div><br />
<div class="leftcon"><p class="blah"><a href="https://2010.igem.org/Team:Tec-Monterrey/Team"><img src="https://static.igem.org/mediawiki/2010/1/13/Teamsmall.png"></a></p> <p class="blah">The Tec-Monterrey iGEM team is made up of ten 7th semester Biotechnology Engineering students from the Tecnológico de Monterrey in Monterrey, Mexico...<a href="https://2010.igem.org/Team:Tec-Monterrey/Team"><b>Read more</b></a></p></div><br />
<div class="rightcon"><p class="blah"><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation"><img src=https://static.igem.org/mediawiki/2010/4/4e/Documsmall.png></a></p><p class="blah">The registry of everything we did in the summer, our lab journal, the protocols, our contribution to human practices… <a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation"><b>Read more</b></a></p></div><br />
<div class="rightcon"><p class="blah"><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus"><img src="https://static.igem.org/mediawiki/2010/e/e3/Bonussmall.png"></a></p><p class="blah">Photos from our summer work and information on our beloved Wiki Wiki… <a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus"><b>Read more</b></a><br />
</p></div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<div class="bgr"><br><br />
</div><br />
</html>\</div>
Andreshuerta
http://2010.igem.org/File:Bonussmall.png
File:Bonussmall.png
2010-10-27T23:45:04Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/File:Documsmall.png
File:Documsmall.png
2010-10-27T23:43:19Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/File:Teamsmall.png
File:Teamsmall.png
2010-10-27T23:39:58Z
<p>Andreshuerta: uploaded a new version of "Image:Teamsmall.png"</p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/File:Teamsmall.png
File:Teamsmall.png
2010-10-27T23:38:41Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey
Team:Tec-Monterrey
2010-10-27T23:32:15Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
color:white;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<style><br />
<br />
<br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<div style="text-align:justify"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/a/a1/Itesmlogo.jpg"><br />
Welcome to the iGEM Tec-Monterrey Team’s Wiki. This is the first time that our university participates in iGEM and we hope that you find our project interesting and enjoy it as much as we enjoyed working on it. Feel free to navigate our wiki and become part of this unforgettable experience.<br />
</p><br />
</div><br />
</div><br />
<div class="ft"><div class="c"></div></div> <br />
<div class="bgr"><br><br />
</div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/9/92/Project.png" border="0" ><br />
<div class="bdy"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/7/73/Semaforo.jpg" width="80"><br />
<br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. The first bacterial reporters appeared 20 years ago, although these early tests didn't use genetically modified microorganisms. Further research and development in the area of genetic engineering has resulted in newer and more sophisticated bacterial sensors, capable of detecting the presence of contaminants, sugars and amino acids in different media such as soil and water. However, most bacterial sensors can only detect the presence of a compound at a certain concentration and currently there are few documented bacterial sensors that can detect and report different concentrations of the compound of interest. Our objective was to develop a genetic frame, compatible with the BioBrick standard, for the creation of a concentration-sensitive bacterial sensor. In the process we also developed and characterized BioBricks for two new families of phage activators. <a href="https://2010.igem.org/Team:Tec-Monterrey/Project"><b>Read More</b></a><br />
<br />
<br />
<br />
<br />
</p><br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div><br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="headercon"><br />
<img src="https://static.igem.org/mediawiki/2010/8/8e/Titulos3.png"><br />
</div><br />
<div class="leftcon"><p class="blah">The Tec-Monterrey iGEM team is made up of ten 7th semester Biotechnology Engineering students from the Tecnológico de Monterrey in Monterrey, Mexico...<a href="https://2010.igem.org/Team:Tec-Monterrey/Team"><b>Read more</b></a></p></div><br />
<div class="rightcon"><p class="blah">The registry of everything we did in the summer, our lab journal, the protocols, our contribution to human practices… <a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation"><b>Read more</b></a></p></div><br />
<div class="rightcon"><p class="blah">Photos from our summer work and information on our beloved Wiki Wiki <a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus"><b>Read more</b></a><br />
</p></div><br />
<div class="footercon">Copyright 1999-2005 by Refsnes Data.</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<div class="bgr"><br><br />
</div><br />
</html>\</div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey
Team:Tec-Monterrey
2010-10-27T23:26:50Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
color:white;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<style><br />
<br />
<br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<div style="text-align:justify"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/a/a1/Itesmlogo.jpg"><br />
Welcome to the iGEM Tec-Monterrey Team’s Wiki. This is the first time that our university participates in iGEM and we hope that you find our project interesting and enjoy it as much as we enjoyed working on it. Feel free to navigate our wiki and become part of this unforgettable experience.<br />
</p><br />
</div><br />
</div><br />
<div class="ft"><div class="c"></div></div> <br />
<div class="bgr"><br><br />
</div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/9/92/Project.png" border="0" ><br />
<div class="bdy"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/7/73/Semaforo.jpg" width="80"><br />
<br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. The first bacterial reporters appeared 20 years ago, although these early tests didn't use genetically modified microorganisms. Further research and development in the area of genetic engineering has resulted in newer and more sophisticated bacterial sensors, capable of detecting the presence of contaminants, sugars and amino acids in different media such as soil and water. However, most bacterial sensors can only detect the presence of a compound at a certain concentration and currently there are few documented bacterial sensors that can detect and report different concentrations of the compound of interest. Our objective was to develop a genetic frame, compatible with the BioBrick standard, for the creation of a concentration-sensitive bacterial sensor. In the process we also developed and characterized BioBricks for two new families of phage activators. <a href="https://2010.igem.org/Team:Tec-Monterrey/Project"><b>Read More</b></a><br />
<br />
<br />
<br />
<br />
</p><br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div><br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="headercon"><br />
<img src="https://static.igem.org/mediawiki/2010/8/8e/Titulos3.png"><br />
</div><br />
<div class="leftcon"><p class="blah">The Tec-Monterrey iGEM team is made up of ten 7th semester Biotechnology Engineering students from the Tecnológico de Monterrey in Monterrey, Mexico...<a href="https://2010.igem.org/Team:Tec-Monterrey/Team"><b>Read more</b></a></p></div><br />
<div class="rightcon"><p>"Never increase, beyond what is necessary, the number of entities required to explain anything." William of Ockham (1285-1349)</p></div><br />
<div class="rightcon"><p>"Never increase, beyond what is necessary, the number of entities required to explain anything." William of Ockham (1285-1349)</p></div><br />
<div class="footercon">Copyright 1999-2005 by Refsnes Data.</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<div class="bgr"><br><br />
</div><br />
</html>\</div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Attributions
Team:Tec-Monterrey/Attributions
2010-10-27T23:25:39Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/8d/Attributions.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<ol class="menud"><br />
<a href="#undergrads"><li>Undergraduates</a></li><br />
<a href="#instructors"><li>Instructors</a></li><br />
<a href="#advisors"><li>Advisors</a></li><br />
<a href="#igemteams"><li>Collaboration with other iGEM Teams</a></li><br />
</ol><br />
</p><br />
<br><br />
<h2><a name="undergrads"></a>Undergraduates</h2><br />
<h4>José Argüelles</h4><br />
<p class="blah"><br />
He investigated theoretical information for the project, performed general laboratory work and collaborated on the design of the synthesis. He was committed to finish the final construct and spent endless nights running gel electrophoreses. <br />
</p> <br />
<h4>Pamela Campos</h4><br />
<p class="blah"><br />
She was in charge of managing and cleaning the material at the laboratory and also helped with general lab work. She contributed by writing the <a href= "https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Importation Manual</a> and by going to the Health Department to request information about the permits necessary to import our DNA synthesis.<br />
</p><br />
<br />
<h4>Cristóbal Cevallos</h4><br />
<p class="blah"><br />
He contacted possible sponsors, organized a fundraising party and was in charge of the trip logistics to Boston. He worked on general laboratory work, mainly in the characterizations of pBAD, MuC and MuMor.<br />
<br />
</p><br />
<br />
<h4>Jan Contreras</h4><br />
<p class="blah"><br />
He performed general laboratory work, focusing in the design of the final construct and the MuMor sensitivity tuner. He did the mathematical modelling and characterization of pBAD, MuC and MuMor sensitivity tuners and participated in the registration of the BioBricks.<br />
<br />
</p><br />
<br />
<h4>Héctor Gálvez</h4><br />
<p class="blah"><br />
He took the leadership of the group and helped organize the work to be done by assigning specific jobs and responsibilities to every team member. He contributed to the logistics of the project and made a calendar with goal dates. He wrote the project summary and he organized most of the information and documentation for the wiki. During the summer he helped with general wet lab work and contacted several government offices for sponsorships.<br />
<br />
</p><br />
<br />
<h4>Andrés Huerta</h4><br />
<p class="blah"><br />
He was in charge of the design and codification of the team wiki, the T-shirts and the poster. He collaborated with general laboratory work and helped in the DNA importation process.<br />
<br />
</p><br />
<br />
<h4>Tatiana Núñez</h4><br />
<p class="blah"><br />
She was in charge of the administration of chemical reactants, general laboratory work, and customs information regarding the DNA importation. She registered and submitted the BioBricks.<br />
<br />
</p><br />
<br />
<h4>Diana Ostos</h4><br />
<p class="blah"><br />
Diana contributed with the internal logistics of the project and ordered and paid the DNA synthesis. She also collaborated with the <a href= "https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Importation Manual</a> and helped with general laboratory work. She worked on the development of the MuC sensitivity tuner and the design of the final construct.<br />
<br />
</p><br />
<br />
<h4>Javier Reynoso</h4><br />
<p class="blah"><br />
During the summer, Javi's main task was to find sponsors for the project, which he achieved quite successfully. He also helped with general laboratory work, but left early for his exchange program in Australia. From there, he still kept tabs with our sponsors. <br />
<br />
</p><br />
<br />
<h4>Flavio Siller</h4><br />
<p class="blah"><br />
He performed mostly general wet lab work. He designed a program to quickly calculate the amount of enzymes needed for digestions and ligation reactions. He also helped with the writing of several parts of the wiki. <br />
<br />
</p><br />
<br />
<h2><a name="instructors"></a>Instructors</h2><br />
<h4>Dr. Mario Álvarez</h4><br />
<p class="blah"><br />
He was the first one to believe in us and suggested ideas for different applications for the sensor. He donated some material from his own projects and will be accompanying us to the Jamboree. <br />
<br />
</p> <br />
<h4>M. Sc. Sergio García</h4><br />
<p class="blah"><br />
Sergio taught us hands-on most of the protocols and procedures we needed in the laboratory - from pipetting correctly to preparing electrocompetent cells. He always had a creative explanation for our troubleshooting and encouraged us to think out of the box. Sergio always supported us with lab material and was incredibly patient with us.<br />
</p><br />
<br />
<h2><a name="advisors"></a>Advisors</h2><br />
<h4>Dr. Manuel Zertuche</h4><br />
<p class="blah"><br />
Dr. Zertuche, on behalf of the Biotechnology and Food Division of the Tecnológico de Monterrey, was the main sponsor of the project and helped us with the planning and the timeline in order to achieve our short and long term goals. He provided us with the facilities and gave us his approval for almost everything we asked for. We really appreciate his advice and support, and thank him for believing in us.<br />
<br />
</p> <br />
<br />
<h4>M. Sc. José Manuel Aguilar</h4><br />
<p class="blah"><br />
He helped us with the theoretical information and developed with us the idea of the intelligent biosensor. He also advised us on troubleshooting. <br />
<br />
</p><br />
<br />
<h4>M. Sc. Johari Salgado</h4><br />
<p class="blah"><br />
Johari selflessly shared his laboratory with us and was always willing to help us get material and equipment.<br />
<br />
</p><br />
<br />
<h4>Felipe López</h4><br />
<p class="blah"><br />
He instructed us on how to use the microplate reader for the characterization of our parts.<br />
</p><br />
<br />
<h2><a name="igemteams"></a>Collaboration with other iGEM Teams</h2><br />
<p class="blah"><br />
We contributed with other universities by participating on their surveys; unfortunately we weren’t able to answer all of them, but we tried to have an active contribution.<br><br />
</p><br />
<h4>Mexico-UNAM-CINVESTAV and UNAM-Genomics_Mexico</h4><br />
<p class="blah"><br />
We contacted and befriended the other two Mexican teams (Mexico-UNAM-CINVESTAV and UNAM-Genomics_Mexico) in order to start an open collaboration in our country and encourage the development of synthetic biology. We exchanged points of view regarding the problems we had with Customs and their advice was taken into account when writing the <a href= "https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Importation Manual</a>. <br><br />
</p><br />
<h4>British Columbia (2009)</h4><br />
<p class="blah"><br />
We used some of the BioBricks submitted by the 2009 British Columbia team (pBAD, pBAD weak, pBAD strong), proved their correct function and re-characterized their <a href="http://partsregistry.org/Part:BBa_I13453">pBAD BioBrick.</a> <br />
</p> <br />
<br />
<h4>TU Delft</h4><br />
<p class="blah"><br />
We would finally like to thank the TU Delft team, as we used part of their code on our wiki. <br />
</p> <br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/File:Itesmlogo.jpg
File:Itesmlogo.jpg
2010-10-27T23:03:28Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/File:Titulos3.png
File:Titulos3.png
2010-10-27T23:00:20Z
<p>Andreshuerta: </p>
<hr />
<div></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey
Team:Tec-Monterrey
2010-10-27T22:13:58Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 19px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<style><br />
<br />
<br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="http://i138.photobucket.com/albums/q274/gnoxyz/iGEM/cbazulplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="http://i138.photobucket.com/albums/q274/gnoxyz/iGEM/legosredondeadoplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="http://i138.photobucket.com/albums/q274/gnoxyz/iGEM/teamredondeadoplano-1.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="http://i138.photobucket.com/albums/q274/gnoxyz/iGEM/electroredondeadoplano-1.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br /><br />
<a href="#">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">Project Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Biosensor">The Biosensor</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Biosensor">Applications</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br /><br />
<a href="#">&nbsp;&nbsp;Notebook</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Notebook">Notebook</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Timeline">Timeline</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Safety">Safety</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br /><br />
<a href="#">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/News">News</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Freetime">Free Time</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="122px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br><br />
<a href="http://www.sukarne.com.mx/"><img src="https://static.igem.org/mediawiki/2010/6/6a/Sukarne.gif" width="120px" height="120px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<div style="text-align:justify"><br />
<p class="blah"><br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a><br />
</p><br><p class="blah"><br />
The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration. <br />
</p><br><p class="blah"><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
</p><br><p class="blah"><br />
With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.<br />
</p><br />
<br />
<br />
<h2>References</h2><br />
<p class="blah"><a name="jaeger">Jaeger, C. H., et. Al. (June 1999)</a> Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690<br />
<br><br><br />
<a name="van">Van der Meer, J. R. and Belkin, S. (July 2010)</a> Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522<br />
<br><br />
<br><br />
<a name="willardson">Willardson, B. M., et. Al. (March 1998)</a> Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012<br />
<br><br><br />
<a name="yagi">Yagi, K. (2007)</a> Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258<br />
<br><br></p><br />
</div><br />
</div><br />
<div class="ft"><div class="c"></div></div> <br />
<div class="bgr"><br><br />
</div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a><br />
</p><br><p class="blah"><br />
The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration. <br />
</p><br><p class="blahimgder"> <br />
<img src=http://t2.gstatic.com/images?q=tbn:CdWigAFIgu8p8M:http://i266.photobucket.com/albums/ii257/durblet/unicorn-puzzle.jpg&t=1><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
</p><br><p class="blahimgizq"><br />
<img src=http://t2.gstatic.com/images?q=tbn:CdWigAFIgu8p8M:http://i266.photobucket.com/albums/ii257/durblet/unicorn-puzzle.jpg&t=1><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
<br />
</p><br><p class="blah"><br />
With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.<br />
</p><br />
<br />
<br />
<h2>References</h2><br />
<p class="blah"><a name="jaeger">Jaeger, C. H., et. Al. (June 1999)</a> Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690<br />
<br><br><br />
<a name="van">Van der Meer, J. R. and Belkin, S. (July 2010)</a> Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522<br />
<br><br />
<br><br />
<a name="willardson">Willardson, B. M., et. Al. (March 1998)</a> Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012<br />
<br><br><br />
<a name="yagi">Yagi, K. (2007)</a> Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258<br />
<br><br></p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> /* Este DIV es el de TEXTEANDO */<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="headercon">W3Schools.com</div><br />
<div class="leftcon"><p>"Never increase, beyond what is necessary, the number of entities required to explain anything." William of Ockham (1285-1349)</p></div><br />
<div class="rightcon"><p>"Never increase, beyond what is necessary, the number of entities required to explain anything." William of Ockham (1285-1349)</p></div><br />
<div class="rightcon"><p>"Never increase, beyond what is necessary, the number of entities required to explain anything." William of Ockham (1285-1349)</p></div><br />
<div class="footercon">Copyright 1999-2005 by Refsnes Data.</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<div class="bgr"><br><br />
</div><br />
</html>\</div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey
Team:Tec-Monterrey
2010-10-27T22:11:50Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<style><br />
<br />
<br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<div style="text-align:justify"><br />
<p class="blah"><br />
Welcome to the iGEM Tec-Monterrey 2010 Wiki. As <br />
</p><br><p class="blah"><br />
The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration. <br />
</p><br><p class="blah"><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
</p><br><p class="blah"><br />
With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.<br />
</p><br />
</div><br />
</div><br />
<div class="ft"><div class="c"></div></div> <br />
<div class="bgr"><br><br />
</div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a><br />
</p><br><p class="blah"><br />
The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration. <br />
</p><br><p class="blahimgder"> <br />
<img src=http://t2.gstatic.com/images?q=tbn:CdWigAFIgu8p8M:http://i266.photobucket.com/albums/ii257/durblet/unicorn-puzzle.jpg&t=1><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
</p><br><p class="blahimgizq"><br />
<img src=http://t2.gstatic.com/images?q=tbn:CdWigAFIgu8p8M:http://i266.photobucket.com/albums/ii257/durblet/unicorn-puzzle.jpg&t=1><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
<br />
</p><br><p class="blah"><br />
With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.<br />
</p><br />
<br />
<br />
<h2>References</h2><br />
<p class="blah"><a name="jaeger">Jaeger, C. H., et. Al. (June 1999)</a> Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690<br />
<br><br><br />
<a name="van">Van der Meer, J. R. and Belkin, S. (July 2010)</a> Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522<br />
<br><br />
<br><br />
<a name="willardson">Willardson, B. M., et. Al. (March 1998)</a> Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012<br />
<br><br><br />
<a name="yagi">Yagi, K. (2007)</a> Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258<br />
<br><br></p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<div style="text-align:justify"><br />
<p class="blah"><br />
Welcome to the iGEM Tec-Monterrey 2010 Wiki. As <br />
</p><br><p class="blah"><br />
The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration. <br />
</p><br><p class="blah"><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
</p><br><p class="blah"><br />
With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.<br />
</p><br />
</div><br />
</div><br />
<div class="ft"><div class="c"></div></div> <br />
<div class="bgr"><br><br />
</div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a><br />
</p><br><p class="blah"><br />
The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration. <br />
</p><br><p class="blahimgder"> <br />
<img src=http://t2.gstatic.com/images?q=tbn:CdWigAFIgu8p8M:http://i266.photobucket.com/albums/ii257/durblet/unicorn-puzzle.jpg&t=1><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
</p><br><p class="blahimgizq"><br />
<img src=http://t2.gstatic.com/images?q=tbn:CdWigAFIgu8p8M:http://i266.photobucket.com/albums/ii257/durblet/unicorn-puzzle.jpg&t=1><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
<br />
</p><br><p class="blah"><br />
With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.<br />
</p><br />
<br />
<br />
<h2>References</h2><br />
<p class="blah"><a name="jaeger">Jaeger, C. H., et. Al. (June 1999)</a> Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690<br />
<br><br><br />
<a name="van">Van der Meer, J. R. and Belkin, S. (July 2010)</a> Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522<br />
<br><br />
<br><br />
<a name="willardson">Willardson, B. M., et. Al. (March 1998)</a> Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012<br />
<br><br><br />
<a name="yagi">Yagi, K. (2007)</a> Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258<br />
<br><br></p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="headercon">W3Schools.com</div><br />
<div class="leftcon"><p>"Never increase, beyond what is necessary, the number of entities required to explain anything." William of Ockham (1285-1349)</p></div><br />
<div class="rightcon"><p>"Never increase, beyond what is necessary, the number of entities required to explain anything." William of Ockham (1285-1349)</p></div><br />
<div class="rightcon"><p>"Never increase, beyond what is necessary, the number of entities required to explain anything." William of Ockham (1285-1349)</p></div><br />
<div class="footercon">Copyright 1999-2005 by Refsnes Data.</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
</html>\</div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Gallery
Team:Tec-Monterrey/Gallery
2010-10-27T15:34:26Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
<br />
/*Credits: Dynamic Drive CSS Library */<br />
/*URL: http://www.dynamicdrive.com/style/ */<br />
<br />
.gallerycontainer{<br />
position: relative;<br />
/*Add a height attribute and set to largest image's height to prevent overlaying*/<br />
}<br />
<br />
.thumbnail img{<br />
border: 1px solid white;<br />
margin: 0 5px 5px 0;<br />
}<br />
<br />
.thumbnail:hover{<br />
background-color: transparent;<br />
}<br />
<br />
.thumbnail:hover img{<br />
border: 1px solid blue;<br />
}<br />
<br />
.thumbnail span{ /*CSS for enlarged image*/<br />
position: absolute;<br />
background-color: lightyellow;<br />
padding: 5px;<br />
left: -1000px;<br />
border: 1px dashed gray;<br />
visibility: hidden;<br />
color: black;<br />
text-decoration: none;<br />
}<br />
<br />
.thumbnail span img{ /*CSS for enlarged image*/<br />
border-width: 0;<br />
padding: 2px;<br />
}<br />
<br />
.thumbnail:hover span{ /*CSS for enlarged image*/<br />
visibility: visible;<br />
top: 0;<br />
left: 230px; /*position where enlarged image should offset horizontally */<br />
z-index: 50;<br />
}<br />
<br />
</style><br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/a/a3/Gallery.png" border="0" ><br />
<br><br><br />
<br><br />
<center><br />
<div id="PictoBrowser101027102244">Get the flash player here: http://www.adobe.com/flashplayer</div><script type="text/javascript" src="http://www.db798.com/pictobrowser/swfobject.js"></script><script type="text/javascript">var so = new SWFObject("http://www.db798.com/pictobrowser.swf", "PictoBrowser", "500", "500", "8", "#DDDDDD"); so.addParam("quality", "low"); so.addParam("scale", "noscale"); so.addParam("align", "mid"); so.addVariable("ids", "igem"); so.addVariable("names", "igem"); so.addVariable("userName", "gnoxyz"); so.addVariable("userId", "55179697@N05"); so.addVariable("source", "keyword"); so.write("PictoBrowser101027102244"); </script><br />
</center><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey_/Sponsors
Team:Tec-Monterrey /Sponsors
2010-10-27T14:52:00Z
<p>Andreshuerta: New page: <html> <head> <meta http-equiv="content-type" content="text/html;charset=utf-8" /> <title>Tec de Monterrey </title> <style> #search-controls { display: none; } </style> </head> ...</p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/5/56/Sponsorstit.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
We would really like to thank our sponsors for their enormous help in making this project a reality. Without their generous support, we wouldn’t have been able to achieve our goals for this project. And to everyone who reads this page, we would like to tell you that all these enterprises provide excellent services and we sincerely recommend their products to anyone interested. For more information you can visit their webpage or contact the team. <br />
<br />
<ol class="menud"><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a>: people we couldn’t have managed without.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Bonus
Team:Tec-Monterrey/Bonus
2010-10-27T14:46:33Z
<p>Andreshuerta: New page: <html> <head> <meta http-equiv="content-type" content="text/html;charset=utf-8" /> <title>Tec de Monterrey </title> <style> #search-controls { display: none; } </style> </head> ...</p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/1/1f/Bonus.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
A more relaxed section to place our photos and the information on our beloved mascot: Wiki Wiki. Here you’ll get how we got along, what we did when we were together and how we became increasingly random as the time passed.<br />
<br />
<ol class="menud"><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a>: photos from the lab and elsewhere.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a>: everything you need to know about Wiki Wiki, our peacock.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Documentation
Team:Tec-Monterrey/Documentation
2010-10-27T14:41:31Z
<p>Andreshuerta: New page: <html> <head> <meta http-equiv="content-type" content="text/html;charset=utf-8" /> <title>Tec de Monterrey </title> <style> #search-controls { display: none; } </style> </head> ...</p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/d/d1/Documentation.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
This is the section in which we registered all we did during the summer: our laboratory journal, the protocols we followed, our considerations on safety & ethics and our contribution to human practices, which was one of our major objectives after encountering some setbacks in the development of our project. Hopefully, the information contained in this section will prove to be very useful for upcoming iGEM teams.<br />
<br />
<ol class="menud"><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a>: all our lab activities, the experiments we carried out to get to our results.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a>: the rules we follow when experimenting.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a>: the team considerations on safety and ethics concerning biological research</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a>: our contribution for the improvement of research conditions in our country.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Team
Team:Tec-Monterrey/Team
2010-10-27T14:30:25Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/f/f4/Teamtit.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
Here you’ll get to know who was part of the project, whether as an undergrad, an instructor or an advisor. Also, you’ll get to know as well the role that every member of the team had in the development of the project, and finally, there is information about our headquarters, the places where we spent most of the summer working and our university.<br />
<br />
<ol class="menud"><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a>: the ten students that took part in this journey.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a>: our guides in the path of synthetic biology</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a>: the role each person played in the team.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a>: the places that became our home: the lab, the Biotechnology Center and our university.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Project
Team:Tec-Monterrey/Project
2010-10-27T14:18:54Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<br />
<br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/9/92/Project.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
In this section you will find all the information related to our iGEM Project, how we came up with the idea, the theory behind our experiments and the results and parts that were obtained after our hard work.<br />
<br />
<ol class="menud"><br />
<a href="#abstract"><li>Project Abstract</a></li><br />
<a href="#overview"><li>Project Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a>: To know more about the basis of synthetic biology and bacterial reporters.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a>: The core of our project and our main proposal.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a>: BioBricks we developed during the project.</li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a>: Our considerations on what will be the next steps to follow and the applications that our project holds for the future.</li><br />
<br />
</ol><br />
<br />
<br />
<br />
<br />
<br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<a name="abstract"></a><img src="https://static.igem.org/mediawiki/2010/7/7e/Abstracttitulo.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. The first bacterial reporters appeared 20 years ago, although these early tests didn't use genetically modified microorganisms. Further research and development in the area of genetic engineering has resulted in newer and more sophisticated bacterial sensors, capable of detecting the presence of contaminants, sugars and amino acids in different media such as soil and water. However, most bacterial sensors can only detect the presence of a compound at a certain concentration and currently there are few documented bacterial sensors that can detect and report different concentrations of the compound of interest. Our objective was to develop a genetic frame, compatible with the BioBrick standard, for the creation of a concentration-sensitive bacterial sensor. In the process we also developed and characterized BioBricks for two new families of phage activators.<br />
<br />
<br />
<br />
<br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<div class="hd"><div class="g"> </div></div><br />
<br />
<div class="top"><br />
<a name="overview"></a><img src="https://static.igem.org/mediawiki/2010/e/ef/Projectoverview.png" border="0" ><br />
<div class="bdy"><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/7/73/Semaforo.jpg"><br />
<h2>Development of a genetic frame for the creation of concentration-sensitive bacterial sensors</h2><br />
</p><br />
<p class="blahimgder"><br />
Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensor, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a><br />
</p><p class="blah"><br />
The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration. <br />
</p><p class="blah"><br />
We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings. <br />
</p><p class="blah"><br />
With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.<br />
</p><br />
<br />
<h2>References</h2><br />
<p class="blah"><a name="jaeger"></a><b>Jaeger, C. H., et. Al. (June 1999)</b> Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690<br />
<br><br><br />
<a name="van"></a><b>Van der Meer, J. R. and Belkin, S. (July 2010)</b> Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522<br />
<br><br />
<br><br />
<a name="willardson"></a><b>Willardson, B. M., et. Al. (March 1998)</b> Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012<br />
<br><br><br />
<a name="yagi"></a><b>Yagi, K. (2007)</b> Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258<br />
<br><br></p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
</div><br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Mascot
Team:Tec-Monterrey/Mascot
2010-10-27T13:19:05Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/48/Wikiwiki.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
</p> <br />
<p class="blah"><br />
<div class="footercon"><br />
<ol class="menud"><br />
<a href="#who"><li>Who is Wiki Wiki?</a></li><br />
<a href="#real"><li>The Real Wiki Wiki</a></li><br />
<a href="#game"><li>The Wiki Wiki Videogame</a></li><br />
</ol><br />
</p><br />
<a name="who"></a><h2>Who is Wiki Wiki?</h2><br />
<p class="blahimgizq"><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/21/Wikiwikip.png"><br />
Wiki Wiki is our team mascot. It is a peacock that lives happily in the school and has achieved its notoriety by squeaking loudly at inadequate moments and by angrily blocking the entrance to our working place, the Biotech Center. <br />
</p><br />
<p class="blahimgizq"><br />
He is quite a random character of the institution and has a family of equally crazy peacocks wandering around campus. Wiki Wiki loves to show off, as you can see on this amateur video of him getting on top of a fence and proving that he totally rules over the school or in this other video where he proves his ownership of the library as well. <br />
</p><br />
<p class="blahimgizq"><br />
Wiki Wiki won over the other school animals (deers, squirrels, ducks, cats...) to be the official iGEM Team Mascot simply because he has way more attitude than the rest of them. While the others walk quietly around campus, being almost imperceptible and boring, Wiki Wiki opens his enormous tail of colorful plumage that intimidates every living creature that dares cross his way. And for that, we truly respect him.<br />
</p><br />
<a name="real"></a><h2>The Real Wiki Wiki</h2><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/8b/N31024065961_1295320_4552.jpg"><br />
<br><br><img src="https://static.igem.org/mediawiki/igem.org/a/a5/Wikiwikicloseup.jpg"><br />
<br><br><img src="https://static.igem.org/mediawiki/igem.org/f/fc/Wiki_wiki.jpg"><br />
</center><br />
<br><br />
<a name="game"></a><h2>The Wiki Wiki Videogame</h2><br />
<p class="blah"><br />
As we waited for electrophoresis, ligations, digestions and other experiments that take quite a while and leave you with little to do, we developed the Wiki Wiki game. Actually, it is an adaptation of <b>Syobon Action</b>, the so-called “Most difficult game ever” with Wiki Wiki as the main character, accompanied with other animals that dwell in our university –squirrels, ducks and deers, mainly- and try to take away Wiki Wiki’s position as the iGEM mascot. Along the way, Wiki Wiki will find a lot of references to iGEM since the ultimate prize is to win the competition.<br />
</p><br />
<p class="blah"><br />
We do not take credit for any of the coding of the game, we only did the character design. The official website of the original game can be visited <a href="http://www.geocities.jp/z_gundam_tanosii/home/Main.html">here</a> (Beware, it is in Japanese and we couldn’t understand a thing). We do not mean by any means to claim that the game belongs to us and if there is any need to take it down, we will, immediately.<br />
</p><br />
<p class="blah"><br />
<b>Screenshots & Characters</b><br />
</p><br />
<br> <br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/26/Videogamewiki.jpg"><br />
</center><br />
<p class="blah"><br />
<br><br />
<a href="http://www.mediafire.com/?eahl17t2lvhna93" target="blank"><b>Download</b></a><br />
</p><br />
<p class="blah"><br />
Instructions: Unrar the archive and just execute the file iGEM.exe.<br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Mascot
Team:Tec-Monterrey/Mascot
2010-10-27T13:17:22Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/48/Wikiwiki.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
</p> <br />
<p class="blah"><br />
<div class="footercon"><br />
<ol class="menud"><br />
<a href="#who"><li>Who is Wiki Wiki?</a></li><br />
<a href="#real"><li>The Real Wiki Wiki</a></li><br />
<a href="#game"><li>The Wiki Wiki Videogame</a></li><br />
</ol><br />
</p><br />
<a name="who"></a><h2>Who is Wiki Wiki?</h2><br />
<p class="blahimgizq"><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/21/Wikiwikip.png"><br />
Wiki Wiki is our team mascot. It is a peacock that lives happily in the school and has achieved its notoriety by squeaking loudly at inadequate moments and by angrily blocking the entrance to our working place, the Biotech Center. <br />
</p><br />
<p class="blahimgizq"><br />
He is quite a random character of the institution and has a family of equally crazy peacocks wandering around campus. Wiki Wiki loves to show off, as you can see on this amateur video of him getting on top of a fence and proving that he totally rules over the school or in this other video where he proves his ownership of the library as well. <br />
</p><br />
<p class="blahimgizq"><br />
Wiki Wiki won over the other school animals (deers, squirrels, ducks, cats...) to be the official iGEM Team Mascot simply because he has way more attitude than the rest of them. While the others walk quietly around campus, being almost imperceptible and boring, Wiki Wiki opens his enormous tail of colorful plumage that intimidates every living creature that dares cross his way. And for that, we truly respect him.<br />
</p><br />
<a name="real"></a><h2>The Real Wiki Wiki</h2><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/8b/N31024065961_1295320_4552.jpg"><br />
<br><br><img src="https://static.igem.org/mediawiki/igem.org/a/a5/Wikiwikicloseup.jpg"><br />
<br><br><img src="https://static.igem.org/mediawiki/igem.org/f/fc/Wiki_wiki.jpg"><br />
</center><br />
<br><br />
<a name="game"></a><h2>The Wiki Wiki Videogame</h2><br />
<p class="blah"><br />
As we waited for electrophoresis, ligations, digestions and other experiments that take quite a while and leave you with little to do, we develop the Wiki Wiki game. Actually, it is an adaptation of <b>Syobon Action</b>, the so-called “Most difficult game ever” with Wiki Wiki as the main character, accompanied with other animals that dwell in our university –squirrels, ducks and deers, mainly- and try to take away Wiki Wiki’s position as the iGEM mascot. Along the way, Wiki Wiki will find a lot of references to iGEM since the ultimate prize is to win the competition.<br />
</p><br />
<p class="blah"><br />
We do not take credit for any of the coding of the game, we only did the character design. The official website of the original game can be visited <a href="http://www.geocities.jp/z_gundam_tanosii/home/Main.html">here</a> (Beware, it is in Japanese and we couldn’t understand a thing). We do not mean by any means to claim that the game belongs to us and if there is any need to take it down, we will, immediately.<br />
</p><br />
<p class="blah"><br />
<b>Screenshots & Characters</b><br />
</p><br />
<br> <br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/26/Videogamewiki.jpg"><br />
</center><br />
<p class="blah"><br />
<br><br />
<a href="http://www.mediafire.com/?eahl17t2lvhna93" target="blank"><b>Download</b></a><br />
</p><br />
<p class="blah"><br />
Instructions: Unrar the archive and just execute the file iGEM.exe.<br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Parts
Team:Tec-Monterrey/Parts
2010-10-27T12:37:22Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
table A:link {text-decoration: none; color: #0D3C6A }<br />
table A:visited {text-decoration: none; color: #0D3C6A}<br />
table A:active {text-decoration: none;color: #0D3C6A}<br />
table A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/7/79/Parts.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
For our project, we submitted ten BioBricks to the Registry of Standard Biological Parts. Most of these parts are related to the two families of PoPS amplifiers that we developed and characterized for our project. Two parts are genetic devices that were used to characterize the PoPS amplifiers. We also submitted our final construction that is the genetic device that powers our “intelligent biosensor”. <br />
</p><br />
<br />
<p class="blah"><br />
<!-- Part Table --><form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<br />
<br />
<!-- Show=Undef --> <br />
<TABLE width='665px'><TR><TD width='50%'><B>Favorite Tec-Monterrey 2010 iGEM Team Parts</B><TD width='35%'><TD width='15%' ALIGN='RIGHT'><A class='noul_link' HREF='http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Tec-Monterrey&Editing=Editing'><I>Edit</I></A></TABLE></form><TABLE class='pgrouptable addborder' style='width: 665px;' cellpadding='0' cellspacing='0'><TR><TH colspan='2' style='width: 1em' nowrap><A style='color:blue;text-decoration:none;'<br />
href='http://partsregistry.org/wiki/index.php/Help:Availability_and _usefulness'>-?-</A><TH style='width: 7em;'>Name</TH><TH colspan=''>Type<TH colspan=''>Description<TH colspan=''>Designer<TH colspan=''>Length<TH class='blank_col'> <br />
<br />
<!-- ROW --> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="20350" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005'>BBa_K427005</A></TD><TD>Device</TD><TD>MuC sensitivity tuner (PoPS->PoPS)</TD><TD width='100px'>Jan Marte Contreras Ortiz</TD><TD ALIGN='right'>628</TD><TD class ='blank_col'></TD></FORM> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="20357" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006'>BBa_K427006</A></TD><TD>Device</TD><TD>MuMor Sensitivity tuner (PoPS->PoPS)</TD><TD width='100px'>Jan Marte Contreras Ortiz</TD><TD ALIGN='right'>587</TD><TD class ='blank_col'></TD></FORM> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="20366" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427007'>BBa_K427007</A></TD><TD>Device</TD><TD>Ter PBadweak Mu C Ter Pmom GFP cassette</TD><TD width='100px'>Tatiana J. Núñez Elizondo</TD><TD ALIGN='right'>1755</TD><TD class ='blank_col'></TD></FORM> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="20364" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427008'>BBa_K427008</A></TD><TD>Device</TD><TD>Ter PBadweak Mu Mor Ter PM GFP cassette</TD><TD width='100px'>Tatiana J. Núñez Elizondo</TD><TD ALIGN='right'>1714</TD><TD class ='blank_col'></TD></FORM></TABLE></FORM><BR><BR> <br />
<br />
<!-- Part Table --><form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<br />
<br />
<!-- Show=Undef --> <br />
<TABLE width='665px'><TR><TD width='50%'><B>Tec-Monterrey 2010 iGEM Team Parts Sandbox</B><TD width='35%'><TD width='15%' ALIGN='RIGHT'><A class='noul_link' HREF='http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Tec-Monterrey&Editing=Editing'><I>Edit</I></A></TABLE></form><TABLE class='pgrouptable addborder' style='width: 665px;' cellpadding='0' cellspacing='0'><TR><TH colspan='2' style='width: 1em' nowrap><A style='color:blue;text-decoration:none;'<br />
href='http://partsregistry.org/wiki/index.php/Help:Availability_and _usefulness'>-?-</A><TH style='width: 7em;'>Name</TH><TH colspan=''>Type<TH colspan=''>Description<TH colspan=''>Designer<TH colspan=''>Length<TH class='blank_col'> <br />
<br />
<!-- ROW --> <br />
<br />
<!-- ROW --> <br />
<br />
<!-- ROW --> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="19804" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427000'>BBa_K427000</A></TD><TD>Regulatory</TD><TD>PBad Weak Reverse</TD><TD width='100px'>Tatiana J. Núñez Elizondo</TD><TD ALIGN='right'>130</TD><TD class ='blank_col'></TD></FORM> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="20349" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427001'>BBa_K427001</A></TD><TD>Translational_Unit</TD><TD>C protein of the Mu bacteriophage</TD><TD width='100px'>Jan Marte Contreras Ortiz</TD><TD ALIGN='right'>438</TD><TD class ='blank_col'></TD></FORM> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="20345" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427002'>BBa_K427002</A></TD><TD>Translational_Unit</TD><TD>Mor protein of Mu bacteriophage</TD><TD width='100px'>Jan Marte Contreras Ortiz</TD><TD ALIGN='right'>405</TD><TD class ='blank_col'></TD></FORM> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="20346" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427003'>BBa_K427003</A></TD><TD>Regulatory</TD><TD>Pm promoter of Mu bacteriophage</TD><TD width='100px'>Jan Marte Contreras Ortiz</TD><TD ALIGN='right'>71</TD><TD class ='blank_col'></TD></FORM> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="20347" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427004'>BBa_K427004</A></TD><TD>Regulatory</TD><TD>Pmom promoter of Mu bacteriophage</TD><TD width='100px'>Jan Marte Contreras Ortiz</TD><TD ALIGN='right'>79</TD><TD class ='blank_col'></TD></FORM> <br />
<br />
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&amp;group=Tec-Monterrey&amp;Done=1" enctype="multipart/form-data"> <br />
<input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="20359" /><input type="hidden" name="pgroup" value="iGEM2010" /><input type="hidden" name="group" value="Tec-Monterrey" /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K427009'>BBa_K427009</A></TD><TD>Device</TD><TD>Intelligent arabinose biosensor with differential response </TD><TD width='100px'>Jan Marte Contreras Ortiz</TD><TD ALIGN='right'>5138</TD><TD class ='blank_col'></TD></FORM></TABLE></FORM><BR> <br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
</div><br />
<br />
<br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Geneticframe
Team:Tec-Monterrey/Geneticframe
2010-10-27T12:32:40Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/5/55/Geneticframe.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<ol class="menud"><br />
<a href="#development"><li>Development of the genetic frame for an “Intelligent Biosensor”<br />
</a></li><br />
<a href="#phage"><li>Phage Activators</a></li><br />
<ol class="menue"><br />
<a href="#proteins"><li>The Proteins</a></li><br />
<a href="#promoters"><li>The Promoters</a></li><br />
<a href="#sensitivity"><li>The Sensitivity Tuners</a></li><br />
<br />
<a href="#characterization"><li>Characterization</a></li><br />
<a href="#muc"><li>MuC sensitivity tuner (BBa_K427005)</a></li><br />
<a href="#mumor"><li>MuMor sensitivity tuner (BBa_K427006)</a></li><br />
<a href="#transfer"><li>Transfer Function Parameters</a></li><br />
</ol><br />
<a href="#references"><li>References</a></li><br />
<br />
</ol><br />
</p><br />
<br><br />
<h2><a name="development"></a> Development of the genetic frame for an “Intelligent Biosensor”</h2><br />
<p class="blah"><br />
After deciding we wanted to make a concentration sensitive biosensor, we began to investigate the different ways we could achieve our goal. After an initial research concerning biological reporters and the mechanisms currently used to make them, we began to read past iGEM projects to look for useful BioBricks that could be applied to our work. Two past iGEM projects were of our particular interest: British Columbia’s 2009 project and Cambridge’s 2007 project. <br />
</p><br />
<br />
<p class="blah"><br />
<ul><br />
<li><b>British Columbia’s project</b><br />
<p class="blah"><br />
In the past iGEM competition The University of British Columbia team developed a project that resembled our initial proposition. They wanted to produce an <i>E. coli</i> that expressed different Fluorescent Proteins depending on the concentration of arabinose in the medium. Basically, they proposed a system that used three different sensitive promoters to activate a “Lock and Key” mechanism that enabled the expression of the Fluorescent Protein. There were 3 promoters: the wild type pBAD promoter that is sensitive to arabinose, and two others called pBAD weak and pBAD strong that were designed by the team via mutagenesis. As their name implies, the weak promoter is sensitive only to higher concentrations of arabinose and the strong promoter is sensitive to lower concentrations of arabinose compared to the wild type. Finally, to prevent the expression of all the fluorescent proteins when there was enough arabinose in the medium to activate all three promoters, they developed a “jammer” that consisted in placing a reverse promoter on the other side of the fluorescent protein. Thus, when both promoters were activated, the production of fluorescent protein ceased. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="https://2009.igem.org/Team:British_Columbia" target="blank">consult their wiki</a>)</i><br />
</p><br />
<br />
<li><b>Cambridge’s project</b><br />
<p class="blah"><br />
In 2007, the University of Cambridge iGEM team worked in developing both intracellular and intercellular communication pathways as well as a Gram-positive platform for synthetic biology. We were particularly interested in the intracellular project. It consisted of a PoPS amplifier made with transcriptional activators and promoters that were obtained from bacteriophage components. The Cambridge team decided to use activators from bacteriophage components because they are not common in the cellular signals of bacteria. In this particular case, they used activator and promoters from the bacteriophage PSP3 and fR73. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="http://parts.mit.edu/igem07/index.php/Cambridge" target="blank">consult their wiki</a>)</i><br />
</p><br />
</p><br />
</ul><br />
<p class="blah"><br />
After reading about these projects we decided that we could adapt our idea and build upon the achievements of those two previous teams. We wanted to contribute in a significant way, so we began by analyzing their results and identifying points that could use more work. We found that, in general, British Columbia’s proposal was complicated and apparently ineffective. The problem seemed to be in the “lock and key” mechanism, which didn’t seem to behave as expected. On the other hand, we found that their “jammers” could be very useful and they seemed to work as expected.<br />
</p><br />
<br />
<p class="blah"><br />
So we began to design of a different genetic frame to create “intelligent biosensors”, one that preferably didn’t contain a “lock and key” mechanism. Our solution is composed by a combination of parts of the two previously mentioned projects. We incorporated the “jammer” mechanism in addition to PoPS amplification systems in order to create a flexible genetic construction for concentration sensitive bio-reporters. Our proposed solution can be seen in Figure 1 (click on it to enlarge): <br />
</p><br />
<br><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/0/0c/Figure1tecp.png"><img src="https://static.igem.org/mediawiki/igem.org/0/0c/Figure1tecp.png" width="650" length="189"></a><br />
<b>Figure 1</b><br />
</center><br />
<br><br />
<p class="blah"><br />
In theory, this genetic construction is able to detect different concentrations of a compound provided that you have three different promoters that are sensitive to different concentrations of the substance. Based on the amount of compound detected, the bacteria will produce one of three reporter proteins. For our project, we decided to use the same sensors and reporter proteins that the University of British Columbia team used in their 2009 iGEM project. Therefore we began with 3 promoters: pBAD wild type, pBAD weak and pBAD strong; each promoter was able to detect arabinose at different concentrations. Our reporter proteins were GFP, YFP, and RFP (Cherry). We arranged the constructions so that GFP is produced when the cells were induced with low concentrations of arabinose, YFP is produced when induced with medium concentrations and RFP (Cherry) is produced when there is a high concentration. The mechanism that enables this behavior will be explained in two parts, the first part involves the detection of the compound and the second part concerns the production of the reporter protein. <br />
</p><br />
<p class="blah"><br />
The first part of the genetic construction is responsible for the detection of arabinose. This is achieved by using a series of promoters from the pBAD family that were developed and characterized by British Columbia. Each promoter is activated at a different concentration of arabinose, the strong promoter requires a higher concentration of arabinose than the weak promoter. When the promoter is activated, it enables the expression of a bacteriophage activator that works as a PoPS amplifier. The first amplifier we used was the one provided by the Cambrdige 2007 team. However, for our construction to work, we required two additional amplifiers. So we did some more research on bacteriophage activators and constructed two new PoPS amplifier systems in order to finish our construction. We consider these new amplifiers to be a valuable addition to the Registry of Standard Biological Parts, because they come from different bacteriophage families, this means that they produce different activator proteins and they can be used at the same time without causing interference. <br />
</p><br />
<p class="blah"><br />
Eventually we realized that if we didn’t have a mechanism that disabled the expression the PoPS amplifiers, when the cells were induced with high concentrations of arabinose, all three reporter proteins would be produced. Once again, incorporating the previous advances of the 2009 British Columbia Team, we were able to overcome this obstacle. The solution consisted in implementing their “jammer” systems, in order to suppress the expression of the PoPS amplifiers as the concentration of arabinose in the medium increases. The full mechanism is explained in the following figures: <br />
</p><br />
<ul><br />
<li>Figure 2: shows the sensor part of the final construct</li><br />
<br><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/2/2a/Figure2p.png"><img src="https://static.igem.org/mediawiki/igem.org/2/2a/Figure2p.png" width="650"></a><br />
<b>Figure 2</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 3: shows the sensor when there is a low concentration of arabinose</li><br />
<br><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/3/30/Figure3p.png"><img src="https://static.igem.org/mediawiki/igem.org/3/30/Figure3p.png" width="650"></a><br />
<b>Figure 3</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 4: shows the sensor when there is a medium concentration of arabinose</li><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/6/6b/Figure4p.png"><img src="https://static.igem.org/mediawiki/igem.org/6/6b/Figure4p.png" width="650"></a><br />
<b>Figure 4</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 5: shows the sensor when there is a high concentration of arabinose</li><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/1/13/Figure5p.png"><img src="https://static.igem.org/mediawiki/igem.org/1/13/Figure5p.png" width="650"></a><br />
<b>Figure 5</b><br />
</center><br><br />
<br />
<p class="blah"><br />
The second part of the construction is in charge of producing the reporter proteins. According to our research on biological sensors, the reporter molecule must be easy to detect and it must be a substance that is not naturally produced by the organism. Fluorescent proteins are great reporter proteins because fulfill both of these requirements. The team from British Columbia University used three different fluorescent proteins in their project in the 2009 competition: Green Fluorescent Protein (GFP), Yellow Fluorescent Protein (YFP), and the Cherry variety of the Red Fluorescent Protein (RFP Cherry). We ultimately decided to use the same three molecules because they have been widely characterized and there is plenty of information regarding their fluorescence spectrum and behavior. <br />
</p><br />
<br />
<p class="blah"><br />
When the sensing part of our construction produces one of the bacteriophage activators, this molecule enables the expression of one of the reporter proteins. Because these promoters are produce have very high PoPS numbers, the amount of reporter molecule produced is elevated, even though the arabinose concentration isn’t. The following figure is a detail that explains the mechanism behind the production of reporter proteins. <br />
</p><br />
<p class="blah"><br />
<ul><br />
<li>Figure 6: shows the reporter part of the final construct. </li><br />
</ul><br />
<br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/7/77/Figure6p.png"><img src="https://static.igem.org/mediawiki/igem.org/7/77/Figure6p.png" width="650"></a><br />
<b>Figure 6</b><br />
</center><br />
</p><br />
<br />
<br />
<br><br />
<br />
<h2><a name="phage"></a>Phage Activators</h2><br />
<p class="blah"><br />
The most basic bio-bricks created in our project were sequences form the genome of the Mu bacteriophage that attacks E. coli. We needed some sort of sensitivity tuner for our biosensor and the registry had only one family of promoters and protein activators that could be used for the construction of these devices. After some research we came to Mu bacteriophage, where we found two different families of promoters and protein activators: Mor protein – Pm promoter and C protein - Plys, Pi, Pp and Pmom promoters. We took the sequences reported sequences of Mor, Pm, C <a href="#kumaraswami">(Kumaraswami & Howe, 2004)</a> and Pmom <a href="#jiang">(Jiang, 2008)</a>. The proteins’ sequences were optimized for their expression in E. coli with Mr. Gene’s optimization algorithm; we added an Elowitzribosome binding site and finally the BioBrick prefix and suffix to create the bio-bricks that we used. We only added the prefix and suffix to the promoters’ sequences.<br />
</p><br />
<a name="proteins"></a><h4>The Proteins</h4><br />
<p class="blah"><br />
Mor and C are both proteins encoded in the genome of Mu bacteriophage, and they have similar characteristics. Both are proteins bind to DNA in a specific sequence, both have about the same size, sequence and have similar secondary structures and domains. Figure 7 <a href="#mathee">(Mathee, 1990)</a> shows the amino acid sequence alignment of Mor and C proteins.<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/81/Morandc.png"><br />
<b>Figure 7</b><br />
</center><br />
<br><br />
<p class="blah"><br />
In that image we can see the similarity between these two proteins, but we can also notice that the helix turn helix motifs are quite different, it is hypothesized that this domains are the ones that recognize and interact with the DNA sequence. According to <a href="#kumaraswami">Kumaraswami (2004)</a> and <a href="#jiang">Jiang (2008)</a> two domains can be observed on each of the proteins a Helix-Turn-Helix (HTH) domain and a dimerization domain, which suggests that the proteins can only activate their promoters when in dimers.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Mor (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427002">BBa_K427002</a>) and C (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427001">BBa_K427001</a>)<br />
</p><br />
<br />
<a name="promoters"></a><h4>The Promoters</h4><br />
<p class="blah"><br />
The first family of activators that we used was the Mor-Pm family. Pm promoter is the middle promoter of the Mu bacteriophage, where it initiates the transcription of C protein. The second family had four promoters: Plys, Pi, Pp and Pmom. We chose Pmom for our sensitivity tuners because out of the four promoters it is the most characterized in literature. It is normally activated by the C protein.<br />
</p><br />
<p class="blah"><br />
The activation of these promoters requires the holenzymeσ^70, which normally binds to the -35 and -10 consensus sequences, these promoters have the -10 but do not have the -35 sequences instead they have another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzymeσ^70 recognizes the promoter and transcription begins.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Pm (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427003">BBa_K427003</a>), Pmom (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427004">BBa_K427004</a>)<br />
</p><br />
<br />
<a name="sensitivity"></a> <h4>The Sensitivity Tuners</h4><br />
<p class="blah"><br />
These devices were designed with two purposes in mind, increase the PoPS output of the pBad promoter (any promoter would work) and change its almost linear production into an almost Boolean one. These parts have the following structure: activator protein, transcriptional stop and phage promoter. The sensitivity tuners can be used to achieve both purposes on any construction they just have to be inserted between the desired promoter and the desired coding sequence.<br />
</p><br />
<p class="blah"><br />
In our project we used three sensitivity tuners, one made by Cambridge in previous iGEMcompetitions;this one is built with phiR73 delta activator (<a href="http://partsregistry.org/Part:BBa_I746352">BBa_I746352</a>), a bidirectional transcriptional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>) and Po phage promoter (<a href="BBa_I746361">BBa_I746361</a>). The other two were built by us using the Mor and C families. They are MuC sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005">BBa_K427005</a>) and MuMor sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006">BBa_K427006</a>). The first one includes MuC protein activator (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427002">BBa_K427002</a>) bidirectional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>) and Pmom promoter (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427004">BBA_K427004</a>). The other includes MuMor protein activator (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427001">BBa_K427001</a>) bidirectional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>) and Pmom promoter (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427003">BBA_K427003</a>).<br />
</p><br />
<br />
<ul class="lista"><br />
<p class="blah"><br />
<li>Figure 8: MuC sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005">BBa_K427005</a>)</li><br />
</ul><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/49/Figure8p.png"><br />
<b>Figure 8</b><br />
</center><br />
</p><br />
<br />
<br />
<ul class="lista"><br />
<p class="blah"><br />
<li>Figure 9: MuMor sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006">BBa_K427006</a>)</li><br />
</ul><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/e/eb/Figure9p.png"><br />
<b>Figure 9</b><br />
</center><br />
</p><br />
<br><br />
<a name="characterization"></a> <h4>Characterization</h4><br />
<p class="blah"><br />
We characterized both sensitivity tuners using Hill equations:</p><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/4d/Hilleq.png"><br />
</center><br />
<p class="blah"><br />
In order to do this we had to build another two parts, they were used only to characterize the sensitivity tuners they have a bidirectional terminator (<a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a>), PBad Weak (<a href="http://partsregistry.org/Part:BBa_K206001">BBa_K206001</a>) the sensitivity tuner (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427005">BBa_K427005</a> or <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427006">BBa_K427006</a>) and GFP reporter (<a href="http://partsregistry.org/Part:BBa_E0840:Experience">BBa_E0840</a>). These parts are <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427007">BBa_K427007</a> for the MuC sensitivity tuner and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K427008">BBa_K427008</a> for the MuMor sensitivity tuner. <br />
</p><br />
<p class="blah"><br />
All the characterizations were made in the BW27783 strain of E. coli. This strain was modified to internalize arabinose so that the concentration inside the cell is the same as the concentration outside. Another modification is that BW27783 does not use or degrade arabinose.The graphs show the data used to obtain the coefficients reported in the table. The protocol we used is described in the Protocol section of our wiki.<br />
</p><br />
<br />
<a name="muc"></a> <h4> MuC sensitivity tuner (BBa_K427005)</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/a/a6/Mucgraph.png"><br />
</center><br />
<br><br />
<a name="mumor"></a> <h4>MuMor sensitivity tuner (BBa_K427006)</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0f/Mumorgraph.png"><br />
</center><br />
<br><br />
<a name="transfer"></a> <h4>Transfer function parameters</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/7/72/Transferfunction.png"><br />
</center><br />
<br><br><br />
<br />
<a name="references"></a> <h2>References</h2><br />
<a name="jiang"></a><b> Jiang, Y. a. (2008).</b> Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.<br />
<br><br><br />
<p class="blah"><a name="kumaraswami"></a><b> Kumaraswami, M., & Howe, M. a.-W. (2004).</b> Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.<br />
<br><br><br />
<a name="mathee"></a><b> Mathee, K. a. (1990).</b> Identification of a Positive Regulator of the Mu Middle Operon. Journal of bacteriology, 6641-6650.<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/SafetyEthics
Team:Tec-Monterrey/SafetyEthics
2010-10-27T12:22:59Z
<p>Andreshuerta: New page: <html> <head> <meta http-equiv="content-type" content="text/html;charset=utf-8" /> <title>Tec de Monterrey </title> <style> #search-controls { display: none; } </style> </head> ...</p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/7/75/Safetyethics.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<ol class="menud"><br />
<a href="#introduction"><li>Introduction to Biosafety</a></li><br />
<a href="#issues"><li>Safety issues in our project</a></li><br />
<a href="#biobricks"><li>Safety issues in our BioBricks</a></li><br />
<a href="#institutional"><li>Institutional safety and regulations</a></li><br />
<a href="#ideas"><li>Ideas for biosafety in synthetic biology</a></li><br />
<br />
</ol><br />
</p><br />
<br><br />
<p class="blah"><br />
As with any new technological advance, Synthetic Biology has been subject to a number of ethical and regulatory procedures to ensure that research in this area is done in a responsible and safe manner. The iGEM Tec-Monterrey team takes the issue of safety very seriously and we conducted a thorough research to understand the underlying philosophical, toxicological and pathological risks that are commonly associated with Synthetic Biology. As part of our project, we analyzed each of our procedures and biological parts in order to ensure that everything was done under the safest conditions possible. We also discussed the issue of biosafety legislation in our institution and our community and we decided to propose a series of new methods that could help to reduce the risk of any security breach when working in this discipline. We invite other iGEM teams to join us in the conversation of Biosafety and together define the path the scientific community should take in these issues. <br />
</p><br />
<h2><a name="introduction"></a> Introduction to Biosafety</h2><br />
<p class="blah"><br />
In order to discuss Biosafety, it is important to understand the concept. Biosafety refers to the prevention of unintentional out brakes of pathogens, toxins due to accidents or lack of knowledge. It is important to distinguish the concept of Biosafety from Biosecurity. Biosecurity deals with issues of theft, misuse, diversion or any intentional release of dangerous biological material or pathogens <a href="#schmidt">(Schmidt, Chapter 6: Do I undestand what I can create? Biosafety issues in synthetic biology, 2009).</a><br />
</p><br />
<br />
<p class="blah"><br />
Although Biosecurity is an important concept, especially when dealing with issues such as bioterrorism and national security, it’s mostly managed by the government and international regulatory agencies. The scientific community can do little or nothing with respect to Biosecurity in the same manner it can only make small contributions to solve problems such as terrorism or organized crime. Most of the times, researchers can limit themselves to denounce irregularities they detect so that a law enforcement agency can assess if it can be considered a Biosecurity breach. However, the issue of Biosafety is almost entirely in the hands of scientists and their research teams. Without the cooperation of the scientific community, governments will have a hard time preventing accidents that can have possible repercussions in public health. <br />
</p><br />
<p class="blah"><br />
In an emerging discipline such as Synthetic Biology, the issue of safety is always front and center because most of the times, even scientists are not sure of potential hazards that can arise from their experiments. However, it has been determined that it is the ethical responsibility of the scientific team to determine possible risks and safety issues that can arise from their projects, and it is expected that they do everything in their power to prevent those risks from materializing into real biological crises. <br />
</p><br />
<br />
<p class="blah"><br />
In current years, there has been an effort to streamline the process of manipulating genes to create synthetic organisms and biological systems. New tools such as the BioBrick standard have made it possible for people without a high degree of specialization to develop new and innovative uses for biological systems. This has led to a series of challenges in the subject of biosafety that need to be addressed immediately since more and more people outside of the traditional scientific community will begin to create self-replicating organisms that will be used in civil and defense applications <a href="#schmidt8">(Schmidt, Difussion of synthetic biology: a challenge to biosafety, 2008)</a>. The situation has become even more critical with the emergence of the so-called biohacker community, a group of people that have begun to lead biological projects from within their own garages and homemade molecular biology laboratories <a href="#ledford">(Ledford, 2010)</a>.<br />
</p><br />
<p class="blah"><br />
<br />
The international community has begun to respond to this new challenge by signing accords such as the Cartagena protocol that has enabled countries to regulate the transfers of Genetically Modified Organisms through their borders. This treaty, signed by the leading nations as well as many developing countries (such as Mexico) was applied for the first time in the year 2003 <a href="#gupta">(Gupta & Falkner, 2006)</a>. However, for many biosecurity experts, this agreement was too little too late and future regulations must be addressed immediately. This has prompted many institutions to launch biosecurity panels as well as research to engineer security measures to prevent any accidents that could put public health in jeopardy. <br />
</p><br />
<br />
<p class="blah"><br />
To this date, there have been no serious accidents in the area of Synthetic Biology, however it is foolish to assume that it will continue to be this way if we don’t do anything to prevent outbreaks. This is especially true since there are still many mechanisms we don’t understand about biological systems and things can get out of control really fast. Therefore, it has become the responsibility of every person involved in the area of Synthetic Biology to contribute and analyze the risks and hazards associated with their projects, in order to prevent any involuntary release of toxins and potentially pathogenic organisms. <br />
</p><br />
<br />
<h2><a name="issues"></a> Safety issues in our project</h2><br />
<br />
<p class="blah"><br />
As we mentioned previously, at the Tecnológico de Monterrey we take the issue of Biosafety very seriously. That’s why when we finally decided on which project we would pursue for the iGEM competition, we began to analyze the potential safety issues that would arise from this experiment. We focused mainly on three aspects: our own safety (researcher safety), the safety of our community (public safety) and the safety of our local ecosystem (environmental safety). <br />
</p><br />
<br />
<p class="blah"><br />
We began by analyzing the potential hazards our project could have for the research team. Because this team would be in direct contact with the synthetic microorganisms it was extremely important to assess any hazards from the beginning to prevent any involuntary security breach. Fortunately, our project dealt with very few toxic chemicals and there were no pathogenic organisms involved. Therefore, by attaining to the basic rules of laboratory work, we could prevent most expositions. The use of latex gloves during the manipulation of bacteria and toxic chemicals, sterilization of all material before and after their use, the strict prohibition of any ingestion of foods inside the laboratory and the disinfection of all working zones before and after laboratory sessions are examples of the rules that we established since the beginning of our work in the wet lab. Thanks to the observance of these rules, we prevented accidents within our lab and fortunately, there were no biosecurity accidents during the span of our project. <br />
</p><br />
<p class="blah"><br />
Secondly, we had to ensure that we weren’t putting our community under any unnecessary risk of exposure to a dangerous microorganism. Even though we never used any pathogenic organism, we did create certain antibiotic resistant strains of E. coli. This can be considered a hazard because even though the bacteria we used were reported to be innocuous, we couldn’t have the certainty that they would remain that way forever. Therefore, disposal of all biological material that was in contact with any of the strains of E. coli we used in the laboratory was autoclaved before its disposal in clearly marked red biohazard bags. The same applied for all liquid residues, especially growth media. Under any circumstance, we disposed of liquids through the drain. All of our liquid residues were collected in containers that were clearly marked as biological residues. Afterwards, these biological residues were handed over to a specialized waste disposal enterprise that was in charge of responsibly dumping the wastes according to the Mexican Sanitary Law. <br />
</p><br />
<p class="blah"><br />
The FEMSA Biotechnology Center is undergoing a process of accreditation that will identify it as a responsible and safety conscious institution. As part of that accreditation, the Center must demonstrate it has a series of safety protocols for laboratory procedures and waste disposal. Most of these safety protocols are developed in order to prevent social and environmental damage. The iGEM team also adhered to these rules and protocols, so we can assure all of our procedures were done with a conscious attempt to reduce environmental damage and contamination. <br />
</p><br />
<br />
<h2><a name="biobricks"></a> Safety issues in our BioBricks</h2><br />
<p class="blah"><br />
Each of our BioBricks was thoroughly analyzed with the help of our Instructors and Advisors to assess if it posed any type of Biosafety hazard. We came to the conclusion that, since most of our parts are related to the amplification of PoPS within a bacterium and most of them don’t involve the secretion of any substance, there weren’t any important biosafety issues to consider when compared to other BioBricks of this type. <br />
</p><br />
<br />
<p class="blah"><br />
It is important, however, to consider the basic safety rules for all handling of BioBricks. This means that special care should be taken to prevent mutations that could lead to the development of dangerous biological parts, try to avoid unnecessary exposition of microorganisms to DNA samples in order to prevent cross contamination that could lead to the creation of antibiotic resistant microorganisms with pathogenic properties. <br />
</p><br />
<br />
<h2><a name="institutional"></a>Institutional safety and regulations</h2><br />
<br />
<br />
<p class="blah"><br />
Mexico is a country that is still in the process of regulating biosafety issues. This means that most of the aspects involved in our project were are not directly mentioned in the law. This creates a great amount of uncertainty that leads to a lack of standardization in laboratory procedures throughout the country. It also means that there is no regulatory or biosafety group that could supervise our project. Therefore we had to rely on the mentorship of our advisors to make sure that our biosafety practices were thorough and sufficient to prevent any undesirable result. <br />
<br />
</p><br />
<br />
<p class="blah"><br />
We based our laboratory biosafety procedures on international standards, specifically those proposed by the United States Health and Safety Executive in The SACGM Compendium of guidance for the Risk assessment of genetically modified microorganisms <a href="#scientific">(Scientific Advisory Committee on Genetic Modification [SACGM], 2009)</a>. When in doubt we consulted with specialists from the FEMSA Biotechnolgy Center and the Center for Sustainable Development of our institution to make sure that we could minimize risk of involuntary exposure to hazardous substances as much as possible. <br />
<br />
</p><br />
<br />
<h2><a name="ideas"></a>Ideas for biosafety in synthetic biology</h2><br />
<br />
<p class="blah"><br />
One thing we think that would be useful to ensure that all teams follow biosafety measures in future iGEM competitions is to create a checklist in the style of a flowchart. This flowchart would include a series of questions pertaining to the different type of projects that can be developed in the area of synthetic biology. Before starting wet-lab work, teams could consult the flowchart in order to know which type of safety measures apply to their specific needs. <br />
<br />
</p><br />
<br />
<p class="blah"><br />
For example, the first question that the flowchart would ask is if the project would concern eukaryotic cells or prokaryotic cells. Depending on the answer, the team would be asked other questions such as if any of the microorganisms used are known pathogens or if they are GRAS organisms. Again, the answers will continue to guide the team until they reach the “end” of the flowchart, and in that dead end they will find a set of important guidelines to consider for their project. These guidelines would be custom made depending on their project and would therefore be easier to apply and follow. <br />
</p><br />
<p class="blah"><br />
All teams could contribute to the construction of the flowchart and the guidelines. However, a team of specialists would supervise the collaboration and make sure that the final guidelines are sufficient to prevent any involuntary outbreaks. Special care should be given to projects that manipulate pathogenic microorganisms or toxic substances. In theory, the flowchart would be updated in each iGEM competition so that it is up to date with the latest information pertaining to the areas of biosafety and biosafety engineering. <br />
</p><br />
<br />
<a name="references"></a> <h2>References</h2><br />
<a name="gupta"></a><b> Gupta, A., & Falkner, R. (2006).</b> The Influence of the Cartagena Protocol on Biosafety: Comparing Mexico, China and South Africa. Global Environmental Politics, 23-55.<br />
<br><br><br />
<a name="ledford"></a><b> Ledford, H. (2010).</b> Lifehackers. Nature, 650-652.<br />
<br><br><br />
<a name="schmidt8"></a><b> Schmidt, M. (2008).</b> Difussion of synthetic biology: a challenge to biosafety. Systems and Synthetic Biology, 1-6.<br />
<br><br><br />
<a name="schmidt"></a><b> Schmidt, M. (2009).</b> Chapter 6: Do I undestand what I can create? Biosafety issues in synthetic biology. In M. Schmidt, A. Kelle, A. Ganguli, & H. de Vriend, Synthetic Biology. The Technoscience and its Societal Consequences (pp. 81-100). Springer Academic Publishing.<br />
<br><br><br />
<a name="scientific"></a><b> Scientific Advisory Committee on Genetic Modification [SACGM]. (2009).</b> The SACGM Compendium of guidance. Part 2: Risk assesment of genetically modified microorganisms (other than those associated with plants). Health and Safety Executive Books.<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Protocols
Team:Tec-Monterrey/Protocols
2010-10-27T11:56:11Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src=https://static.igem.org/mediawiki/2010/3/35/Media.png border="0" ><br />
<div class="bdy"><br />
<br><p class="blahimgder"><br />
<img src=https://static.igem.org/mediawiki/2010/e/e5/Mediolb.jpg><br />
<ul class="lista"><br />
<li><b>SOB:</b> The protocol found on <a href="http://openwetware.org/wiki/SOB" target="_blank">OpenWetWare</a> was used to prepare SOB Medium.</li><br />
<li><b>SOC:</b> The protocol found on <a href="http://openwetware.org/wiki/SOC" target="_blank">OpenWetWare</a> was used to prepare SOC Medium.</li><br />
<li><b>M9 Minimal Medium:</b> The protocol found on <a href="http://openwetware.org/wiki/M9_medium/minimal" target="_blank">OpenWetWare</a> was used to prepare M9 Minimal Medium.</li><br />
<li><b>LB:</b> The protocol found on LB Broth Base, powder by Invitrogen was used to prepare LB Medium (20 g per liter of distilled water).</li><br />
</ul><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div> <br />
<div class="bgr"><br><br />
</div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<br />
<br />
<img src="https://static.igem.org/mediawiki/2010/b/b8/Protocolstitle.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<img src="https://static.igem.org/mediawiki/2010/6/6d/Protocoloss.jpg"><br />
<ol class="menud"><br />
<a href="#extraction"><li>Plasmid DNA Extraction</a></li><br />
<a href="#restriction"><li>Restriction Digestion</a></li><br />
<a href="#electro"> <li>Agarose Gel Electrophoresis</a></li><br />
<a href="#purif"><li>Purification of DNA Fragments from an Agarose Gel </a></li><br />
<a href="#ligation"><li>DNA Ligation</a></li><br />
<a href="#competent"><li>Preparation of Electrocompetent Cells </a></li><br />
<a href="#transformation"><li>Transformation via Electroporation</a></li><br />
<a href="#characterization"><li>Characterization</a></li><br />
</ol><br />
</p><br />
<br><br />
<h2><a name="extraction"></a>Plasmid DNA Extraction</h2><br />
<p class="blah"><br />
<a href="https://static.igem.org/mediawiki/2010/e/e7/Tb225.pdf" target=blank>Wizard® Plus SV Minipreps DNA Purification System</a> was used for DNA extraction. Slight changes were made to the Centrifugation protocol: <br />
</p><br />
<p class="blah"><br />
<b>Production of Cleared Lysate</b><br />
<ol class="listad"><br />
<li>Pellet 20 mL of overnight culture for 15 minutes at 8,000 x g.</li><br />
</ol><br />
<b>Washing</b><br />
<ol><br />
<li value="12"> Centrifuge for 2 minutes at 14,000 x rpm with the microcentrifuge lid open to allow evaporation of any residual ethanol.</li><br />
</ol><br />
<b>Elution</b><br />
<ol><br />
<li value="14"> Add 50 μL of Nuclease-Free Water to the Spin Column. Centrifuge at 14,000 rpm for 1 minute at room temperature.</li><br />
</ol><br />
</p><br />
<h2><a name="restriction"></a>Restriction Digestion</h2><br />
<p class="blah"><br />
<ol class="listad"><br />
<li>Review the Digestion Calculator, available through <a href="https://static.igem.org/mediawiki/2010/6/60/Digestion_Calculator.xls" target="blank">here</a>, to make the proper calculations. Adjust the final volume of the reaction and be sure to add the DNA concentration and the proper enzymes to be used (with their corresponding quantities).</li><br />
<li>Add all of the components but the enzymes to a 0.2 ml PCR tube. Make sure that the buffer used is the one recommended for the combination of enzymes to be used.</li><br />
<li>Carefully, add the enzymes, preferably without taking them out of the freezer.</li><br />
<li>Incubate the tubes at 37˚C for two hours.</li><br />
</ol><br />
</p><br />
<h2><a name="electro"></a>Agarose Gel Electrophoresis</h2><br />
<p class="blah"><br />
The following procedure is for the preparation of a 1% agarose gel. <br />
</p><br />
<p class="blah"><b>Materials:</b><br />
<ul class="lista"><br />
<li>Agarose (Electrophoresis grade)</li><br />
<li>1X TAE</li><br />
<li>Electrophoresis chamber</li><br />
<li>Electrophoresis tray</li><br />
<li>Transilluminator</li><br />
<li>SYBR Safe</li><br />
<li>Microwave</li><br />
<li>Beaker</li><br />
</ul><br />
</p><br />
<p class="blah"><b>Procedure:</b><br />
<ol class="listad"><br />
<li>Take a beaker and place 30 mL of 1X TAE.</li><br />
<li>Weigh 0.3 gr of agarose and add it to 1X TAE solution. </li><br />
<li>Place beaker inside microwave and heat it until agarose dissolves completely. </li><br />
<li>Allow agarose to cool down to 60°C and add 3 μL of SYBR Safe 10 000X. </li><br />
<li>Mix solution and pour it in a taped gel tray. </li><br />
<li>Add a comb and let the gel harden for about 15 minutes. </li><br />
<li>Remove the comb and the tape and put the gel tray in the electrophoresis chamber. </li><br />
<li>Add enough 1X TAE to completely cover the gel. </li><br />
<li>Add DNA loading buffer to the samples and load them. </li><br />
<li>Run the gel at a voltage between 60V and 120V, depending on the desired resolution/time. </li><br />
<li>Visualize or cut the DNA by putting it in the transilluminator. </li><br />
</ol><br />
</p><br />
<h2> <a name="purif"></a>Purification of DNA Fragments from an Agarose Gel</h2><br />
<p class="blah"><br />
<a href="https://static.igem.org/mediawiki/2010/1/1a/Fb072.pdf" target="blank">Wizard® SV Gel and PCR Clean-Up System</a> was used for purification of DNA fragments obtained from an agarose gel or ligation reaction. Slight changes were made to the DNA Purification by Centrifugation protocol: <br />
</p><br />
<p class="blah"><br />
<b>Washing</b><br><br />
<ol class="listad"><br />
<li value="6">Empty the Collection Tube, incubate at room temperature for 5 minutes and recentrifuge the column assembly for 2 minutes at 8,000 x rpm to allow evaporation of any residual ethanol.</li><br />
</ol><br />
<b>Elution</b><br><br />
<ol class="listad"><br />
<li value="8">Add 30 μL of Nuclease-Free Water to the Minicolumn. Incubate at room temperature for 1 minute. Centrifuge at 16,000 x g for 3 minutes.</li><br />
</ol><br />
</p><br />
<h2> <a name="ligation"></a>DNA Ligation</h2><br />
<p class="blah"><br />
<a href="https://static.igem.org/mediawiki/2010/6/6d/T4dnaligase_5U_man.pdf" target="blank">T4 DNA Ligase protocol</a> by Invitrogen was used to ligate DNA, aided by a Ligation Calculator available through <a href="https://static.igem.org/mediawiki/2010/8/8e/Ligation_reaction.xls" target="blank">here</a>.</p><br />
</p><br />
<h2><a name="competent"></a>Preparation of Electrocompetent Cells</h2><br />
<p class="blah"><br />
The following protocol is for preparing electrocompetent <i>E.coli</i> cells.<br />
</p><br />
<b>Materials:</b><br />
<ul class="lista"><br />
<li>LB-agar plate</li><br />
<li>LB medium</li><br />
<li>500 mL chilled autoclaved dH2O, stored at 4°C</li><br />
<li>Chilled 10% glycerol/dH2O solution, stored at 4°C</li><br />
<li>1 L flask</li><br />
<li>50 ml tubes</li><br />
<li>0.2 mL tubes</li><br />
<li>Ice bucket</li><br />
<li>Ice</li><br />
<li>Refrigerated centrifuge</li><br />
</ul><br />
<p class="blah"><br />
<b>Procedure:</b><br />
<ol class="listad"><br />
<li>Streak <i>Escherichia coli</i> DH5α cells onto LB-agar plate with no antibiotics and incubate at 37°C overnight. </li><br />
<li>Pick one colony and place it in a 50 mL tube with 20 mL LB medium. Incubate overnight on a shaker at 37°C and 350 rpm. </li><br />
<li>Add 250 ml of LB medium to a flask and add the overnight culture until an OD600 of 0.1 is reached. </li><br />
<li>Place the flask on a shaker at 37°C, 350 rpm until an OD600 between 0.4-0.6 is reached. </li> <br />
<li>Transfer the diluted culture to 50 mL tubes. </li><br />
<li>After this step, the cells must be kept at 4°C at all times. Place the cells on ice for 15 minutes. </li><br />
<li>Cool the centrifuge to 4°C. </li><br />
<li>Centrifuge the tubes for 10 min at 8000g at 4°C. </li><br />
<li>Remove supernatant and gently resuspend pellets with 10 mL cold sterile water by pipetting. Add the rest of the water to a total volume of 50 mL. </li><br />
<li>Centrifuge a second time for 10 min at 8000g at 4°C. </li><br />
<li>Remove supernatant and gently resuspend pellets with 10 mL cold sterile water by pipetting. Add the rest of the water to a total volume of 50 mL. </li><br />
<li>Centrifuge a third time for 10 min at 8000g rpm at 4°C. </li><br />
<li>Remove supernatant and gently resuspend pellets with the remaining water (if it’s too little, add some more). </li><br />
<li>Calculate and add glycerol so that the final concentration is 10-15 %. </li><br />
<li>Resuspend the cells and aliquot 50 μL per 0.2 mL tube (tubes on ice) and store at -80°C. </li><br />
</ol><br />
</p><br />
<h2><a name="transformation"></a>Transformation via Electroporation</h2><br />
<p class="blah"><br />
The following protocol is for transforming plasmid DNA into <i>Escherichia coli</i> DH5α cells. </p><br />
<b>Materials:</b><br />
<ul class="lista"><br />
<li>Electrocompetent cells</li><br />
<li>Plasmid DNA (from a ligation reaction) </li><br />
<li>Ice </li><br />
<li>Ice bucket</li><br />
<li>Electroporation cuvette (2mm gap width) </li><br />
<li>Electroporator (Biorad Gene Pulser Xcell) </li><br />
<li>LB-agar plate with appropriate antibiotic </li><br />
<li>1mL SOC at room-temperature</li><br />
</ul><br />
<p class="blah"><br />
<b>Procedure:</b><br />
<ol class="listad"><br />
<li>Chill electroporation cuvettes, DNA samples and tubes on ice. </li><br />
<li>Once cuvettes are cold, remove electrocompetent cells from -80°C freezer and thaw on ice. </li><br />
<li>Turn on electroporator and set voltage to 2.5 kV. </li><br />
<li>Dial a micropipette to 1 or 2μL of DNA sample. </li><br />
<li>Dial a micropipette to 50μL of electrocompetent cells. </li><br />
<li>Dial a micropipette to 1000μL and pipet in SOC. Place micropipette on counter such that tip doesn't touch anything. </li><br />
<li>Pipet 1-2μL of DNA sample and place inside the cuvette. </li><br />
<li>Pipet 50μL of electrocompetent cells inside the cuvette ensuring they mix with the DNA sample. Do not pipet up and down. </li><br />
<li>Place cuvette back on ice to ensure it remains cold. </li><br />
<li>Tap the cuvette on the counter gently so that cells are at the bottom and to remove any air bubbles. </li><br />
<li>Wipe off excess moisture from outside of cuvette. </li><br />
<li>Place in chamber of electroporator so that the cuvette sits between electrodes. </li><br />
<li>Pulse the cells with a shock by pressing button on electroporator. </li><br />
<li>Remove cuvette from the chamber and immediately add SOC. </li><br />
<li>Transfer cuvette to 37°C incubator and shake at 350 rpm to promote aeration. Incubate for 1 hr. </li><br />
<li>Plate 100 μL transformation onto LB-agar plate supplemented with appropriate antibiotic. </li><br />
<li>Incubate plate overnight at 37°C until colonies appear. </li><br />
</ol><br />
</p><br />
<br />
<h2><a name="characterization"></a>Characterization</h2><br />
<p class="blah"><br />
The following protocol is for characterizing the PoPS amplifier by measuring absorbance and fluorescence.<br />
<br />
</p><br />
<b>Materials:</b><br />
<ul class="lista"><br />
<li>LB medium</li><br />
<li>50 ml tubes</li><br />
<li>Antibiotic</li><br />
<li>Incubator</li><br />
<li>Spectrophotometer</li><br />
<li>Microplate reader</li><br />
<li>Clear 96-well microplate</li><br />
<li>0.1, 0.5, 1, 10, 50, 100 and 500 μM L-Arabinose solutions</li><br />
</ul><br />
<p class="blah"><br />
<b>Procedure:</b><br />
<ol class="listad"><br />
<li>Pick three different colonies that contain the desired part to be characterized and place each of them in a different 50 mL tube with 5 mL LB medium.</li><br />
<li>Add 1 μL of the corresponding antibiotic per each mL of LB medium. </li><br />
<li>Pick one colony that does not contain the plasmid with the part that will be characterized (control) and place it in a50 mL tube with 5 mL LB medium. </li><br />
<li>Incubate all the 4 tubes for 16 hours on a shaker at 37°C and 350 rpm. </li><br />
<li>Check the OD600 of the cultures and dilute with fresh LB medium until an OD600 of 0.1 is reached. Make sure that you have at least 7 mL of each culture. </li><br />
<li>Add 1 μL of the corresponding antibiotic per each mL of LB medium. </li><br />
<li>Incubate all the 4 tubes on a shaker at 37°C and 350 rpm until an OD600 of 0.6 is reached. </li><br />
<li>Fill each well with 198 μl of inoculum and 2 μl of L-Arabinose at different concentrations. Make 3 repetitions of each colony with the different concentrations of L-Arabinose. </li><br />
<li>The microplate is then read by the microplate reader by using the following protocol: </li><br />
<ol class="menue"><br />
<li>Set temperature to 37°C</li><br />
<li>Kinetic reading lasting 4 hours with measurements every 5 minutes. </li><br />
<li>Absorbance (600 nm filter) and Fluorescence (Excitation: 485 nm, Emission: 528 nm). </li><br />
<li>Shaking in intensity 2 for 5 seconds before every reading. </li><br />
</ol><br />
<li>Export the results of the well data to an Excel sheet for further interpretation. </li><br />
</ol><br />
</p><br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Geneticframe
Team:Tec-Monterrey/Geneticframe
2010-10-27T11:46:41Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/5/55/Geneticframe.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<ol class="menud"><br />
<a href="#development"><li>Development of the genetic frame for an “Intelligent Biosensor”<br />
</a></li><br />
<a href="#phage"><li>Phage Activators</a></li><br />
<ol class="menue"><br />
<a href="#proteins"><li>The Proteins</a></li><br />
<a href="#promoters"><li>The Promoters</a></li><br />
<a href="#sensitivity"><li>The Sensitivity Tuners</a></li><br />
<br />
<a href="#characterization"><li>Characterization</a></li><br />
<a href="#muc"><li>MuC sensitivity tuner (BBa_K427005)</a></li><br />
<a href="#mumor"><li>MuMor sensitivity tuner (BBa_K427006)</a></li><br />
<a href="#transfer"><li>Transfer Function Parameters</a></li><br />
</ol><br />
<a href="#references"><li>References</a></li><br />
<br />
</ol><br />
</p><br />
<br><br />
<h2><a name="development"></a> Development of the genetic frame for an “Intelligent Biosensor”</h2><br />
<p class="blah"><br />
After deciding we wanted to make a concentration sensitive biosensor, we began to investigate the different ways we could achieve our goal. After an initial research concerning biological reporters and the mechanisms currently used to make them, we began to read past iGEM projects to look for useful BioBricks that could be applied to our work. Two past iGEM projects were of our particular interest: British Columbia’s 2009 project and Cambridge’s 2007 project. <br />
</p><br />
<br />
<p class="blah"><br />
<ul><br />
<li><b>British Columbia’s project</b><br />
<p class="blah"><br />
In the past iGEM competition The University of British Columbia team developed a project that resembled our initial proposition. They wanted to produce an <i>E. coli</i> that expressed different Fluorescent Proteins depending on the concentration of arabinose in the medium. Basically, they proposed a system that used three different sensitive promoters to activate a “Lock and Key” mechanism that enabled the expression of the Fluorescent Protein. There were 3 promoters: the wild type pBAD promoter that is sensitive to arabinose, and two others called pBAD weak and pBAD strong that were designed by the team via mutagenesis. As their name implies, the weak promoter is sensitive only to higher concentrations of arabinose and the strong promoter is sensitive to lower concentrations of arabinose compared to the wild type. Finally, to prevent the expression of all the fluorescent proteins when there was enough arabinose in the medium to activate all three promoters, they developed a “jammer” that consisted in placing a reverse promoter on the other side of the fluorescent protein. Thus, when both promoters were activated, the production of fluorescent protein ceased. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="https://2009.igem.org/Team:British_Columbia" target="blank">consult their wiki</a>)</i><br />
</p><br />
<br />
<li><b>Cambridge’s project</b><br />
<p class="blah"><br />
In 2007, the University of Cambridge iGEM team worked in developing both intracellular and intercellular communication pathways as well as a Gram-positive platform for synthetic biology. We were particularly interested in the intracellular project. It consisted of a PoPS amplifier made with transcriptional activators and promoters that were obtained from bacteriophage components. The Cambridge team decided to use activators from bacteriophage components because they are not common in the cellular signals of bacteria. In this particular case, they used activator and promoters from the bacteriophage PSP3 and fR73. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="http://parts.mit.edu/igem07/index.php/Cambridge" target="blank">consult their wiki</a>)</i><br />
</p><br />
</p><br />
</ul><br />
<p class="blah"><br />
After reading about these projects we decided that we could adapt our idea and build upon the achievements of those two previous teams. We wanted to contribute in a significant way, so we began by analyzing their results and identifying points that could use more work. We found that, in general, British Columbia’s proposal was complicated and apparently ineffective. The problem seemed to be in the “lock and key” mechanism, which didn’t seem to behave as expected. On the other hand, we found that their “jammers” could be very useful and they seemed to work as expected.<br />
</p><br />
<br />
<p class="blah"><br />
So we began to design of a different genetic frame to create “intelligent biosensors”, one that preferably didn’t contain a “lock and key” mechanism. Our solution is composed by a combination of parts of the two previously mentioned projects. We incorporated the “jammer” mechanism in addition to PoPS amplification systems in order to create a flexible genetic construction for concentration sensitive bio-reporters. Our proposed solution can be seen in Figure 1 (click on it to enlarge): <br />
</p><br />
<br><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/0/0c/Figure1tecp.png"><img src="https://static.igem.org/mediawiki/igem.org/0/0c/Figure1tecp.png" width="650" length="189"></a><br />
<b>Figure 1</b><br />
</center><br />
<br><br />
<p class="blah"><br />
In theory, this genetic construction is able to detect different concentrations of a compound provided that you have three different promoters that are sensitive to different concentrations of the substance. Based on the amount of compound detected, the bacteria will produce one of three reporter proteins. For our project, we decided to use the same sensors and reporter proteins that the University of British Columbia team used in their 2009 iGEM project. Therefore we began with 3 promoters: pBAD wild type, pBAD weak and pBAD strong; each promoter was able to detect arabinose at different concentrations. Our reporter proteins were GFP, YFP, and RFP (Cherry). We arranged the constructions so that GFP is produced when the cells were induced with low concentrations of arabinose, YFP is produced when induced with medium concentrations and RFP (Cherry) is produced when there is a high concentration. The mechanism that enables this behavior will be explained in two parts, the first part involves the detection of the compound and the second part concerns the production of the reporter protein. <br />
</p><br />
<p class="blah"><br />
The first part of the genetic construction is responsible for the detection of arabinose. This is achieved by using a series of promoters from the pBAD family that were developed and characterized by British Columbia. Each promoter is activated at a different concentration of arabinose, the strong promoter requires a higher concentration of arabinose than the weak promoter. When the promoter is activated, it enables the expression of a bacteriophage activator that works as a PoPS amplifier. The first amplifier we used was the one provided by the Cambrdige 2007 team. However, for our construction to work, we required two additional amplifiers. So we did some more research on bacteriophage activators and constructed two new PoPS amplifier systems in order to finish our construction. We consider these new amplifiers to be a valuable addition to the Registry of Standard Biological Parts, because they come from different bacteriophage families, this means that they produce different activator proteins and they can be used at the same time without causing interference. <br />
</p><br />
<p class="blah"><br />
Eventually we realized that if we didn’t have a mechanism that disabled the expression the PoPS amplifiers, when the cells were induced with high concentrations of arabinose, all three reporter proteins would be produced. Once again, incorporating the previous advances of the 2009 British Columbia Team, we were able to overcome this obstacle. The solution consisted in implementing their “jammer” systems, in order to suppress the expression of the PoPS amplifiers as the concentration of arabinose in the medium increases. The full mechanism is explained in the following figures: <br />
</p><br />
<ul><br />
<li>Figure 2: shows the sensor part of the final construct</li><br />
<br><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/2/2a/Figure2p.png"><img src="https://static.igem.org/mediawiki/igem.org/2/2a/Figure2p.png" width="650"></a><br />
<b>Figure 2</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 3: shows the sensor when there is a low concentration of arabinose</li><br />
<br><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/3/30/Figure3p.png"><img src="https://static.igem.org/mediawiki/igem.org/3/30/Figure3p.png" width="650"></a><br />
<b>Figure 3</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 4: shows the sensor when there is a medium concentration of arabinose</li><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/6/6b/Figure4p.png"><img src="https://static.igem.org/mediawiki/igem.org/6/6b/Figure4p.png" width="650"></a><br />
<b>Figure 4</b><br />
</center><br />
<br><br />
<ul><br />
<li>Figure 5: shows the sensor when there is a high concentration of arabinose</li><br />
</ul><br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/1/13/Figure5p.png"><img src="https://static.igem.org/mediawiki/igem.org/1/13/Figure5p.png" width="650"></a><br />
<b>Figure 5</b><br />
</center><br><br />
<br />
<p class="blah"><br />
The second part of the construction is in charge of producing the reporter proteins. According to our research on biological sensors, the reporter molecule must be easy to detect and it must be a substance that is not naturally produced by the organism. Fluorescent proteins are great reporter proteins because fulfill both of these requirements. The team from British Columbia University used three different fluorescent proteins in their project in the 2009 competition: Green Fluorescent Protein (GFP), Yellow Fluorescent Protein (YFP), and the Cherry variety of the Red Fluorescent Protein (RFP Cherry). We ultimately decided to use the same three molecules because they have been widely characterized and there is plenty of information regarding their fluorescence spectrum and behavior. <br />
</p><br />
<br />
<p class="blah"><br />
When the sensing part of our construction produces one of the bacteriophage activators, this molecule enables the expression of one of the reporter proteins. Because these promoters are produce have very high PoPS numbers, the amount of reporter molecule produced is elevated, even though the arabinose concentration isn’t. The following figure is a detail that explains the mechanism behind the production of reporter proteins. <br />
</p><br />
<p class="blah"><br />
<ul><br />
<li>Figure 6: shows the reporter part of the final construct. </li><br />
</ul><br />
<br />
<center><br />
<a href="https://static.igem.org/mediawiki/igem.org/7/77/Figure6p.png"><img src="https://static.igem.org/mediawiki/igem.org/7/77/Figure6p.png" width="650"></a><br />
<b>Figure 6</b><br />
</center><br />
</p><br />
<br />
<br />
<br><br />
<br />
<h2><a name="phage"></a>Phage Activators</h2><br />
<p class="blah"><br />
The most basic bio-bricks created in our project were sequences form the genome of the Mu bacteriophage that attacks E. coli. We needed some sort of sensitivity tuner for our biosensor and the registry had only one family of promoters and protein activators that could be used for the construction of these devices. After some research we came to Mu bacteriophage, where we found two different families of promoters and protein activators: Mor protein – Pm promoter and C protein - Plys, Pi, Pp and Pmom promoters. We took the sequences reported sequences of Mor, Pm, C <a href="#kumaraswami">(Kumaraswami & Howe, 2004)</a> and Pmom <a href="#jiang">(Jiang, 2008)</a>. The proteins’ sequences were optimized for their expression in E. coli with Mr. Gene’s optimization algorithm; we added an Elowitzribosome binding site and finally the BioBrick prefix and suffix to create the bio-bricks that we used. We only added the prefix and suffix to the promoters’ sequences.<br />
</p><br />
<a name="proteins"></a><h4>The Proteins</h4><br />
<p class="blah"><br />
Mor and C are both proteins encoded in the genome of Mu bacteriophage, and they have similar characteristics. Both are proteins bind to DNA in a specific sequence, both have about the same size, sequence and have similar secondary structures and domains. Figure 7 <a href="#mathee">(Mathee, 1990)</a> shows the amino acid sequence alignment of Mor and C proteins.<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/81/Morandc.png"><br />
<b>Figure 7</b><br />
</center><br />
<br><br />
<p class="blah"><br />
In that image we can see the similarity between these two proteins, but we can also notice that the helix turn helix motifs are quite different, it is hypothesized that this domains are the ones that recognize and interact with the DNA sequence. According to <a href="#kumaraswami">Kumaraswami (2004)</a> and <a href="#jiang">Jiang (2008)</a> two domains can be observed on each of the proteins a Helix-Turn-Helix (HTH) domain and a dimerization domain, which suggests that the proteins can only activate their promoters when in dimers.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Mor (BBa_K427002) and C (BBa_K427001)<br />
</p><br />
<br />
<a name="promoters"></a><h4>The Promoters</h4><br />
<p class="blah"><br />
The first family of activators that we used was the Mor-Pm family. Pm promoter is the middle promoter of the Mu bacteriophage, where it initiates the transcription of C protein. The second family had four promoters: Plys, Pi, Pp and Pmom. We chose Pmom for our sensitivity tuners because out of the four promoters it is the most characterized in literature. It is normally activated by the C protein.<br />
</p><br />
<p class="blah"><br />
The activation of these promoters requires the holenzymeσ^70, which normally binds to the -35 and -10 consensus sequences, these promoters have the -10 but do not have the -35 sequences instead they have another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzymeσ^70 recognizes the promoter and transcription begins.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Pm (BBa_K427003), Pmom (BBa_K427004)<br />
</p><br />
<br />
<a name="sensitivity"></a> <h4>The Sensitivity Tuners</h4><br />
<p class="blah"><br />
These devices were designed with two purposes in mind, increase the PoPS output of the pBad promoter (any promoter would work) and change its almost linear production into an almost Boolean one. These parts have the following structure: activator protein, transcriptional stop and phage promoter. The sensitivity tuners can be used to achieve both purposes on any construction they just have to be inserted between the desired promoter and the desired coding sequence.<br />
</p><br />
<p class="blah"><br />
In our project we used three sensitivity tuners, one made by Cambridge in previous iGEMcompetitions;this one is built with phiR73 delta activator (BBa_I746352), a bidirectional transcriptional terminator (BBa_B0014) and Po phage promoter (BBa_I746361). The other two were built by us using the Mor and C families. They are MuC sensitivity tuner (BBa_K427005) and MuMor sensitivity tuner (BBa_K427006). The first one includes MuC protein activator (BBa_K427002) bidirectional terminator (BBa_B0014) and Pmom promoter (BBA_K427004). The other includes MuMor protein activator (BBa_K427001) bidirectional terminator (BBa_B0014) and Pmom promoter (BBA_K427003).<br />
</p><br />
<p class="blah"><br />
<ul><br />
<li>Figure 8: MuC sensitivity tuner (BBa_K427005)</li><br />
</ul><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/49/Figure8p.png"><br />
<b>Figure 8</b><br />
</center><br />
</p><br />
<br />
<p class="blah"><br />
<ul><br />
<li>Figure 9: MuMor sensitivity tuner (BBa_K427006)</li><br />
</ul><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/e/eb/Figure9p.png"><br />
<b>Figure 9</b><br />
</center><br />
</p><br />
<br><br />
<a name="characterization"></a> <h4>Characterization</h4><br />
<p class="blah"><br />
We characterized both sensitivity tuners using Hill equations:</p><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/4d/Hilleq.png"><br />
</center><br />
<p class="blah"><br />
In order to do this we had to build another two parts, they were used only to characterize the sensitivity tuners they have a bidirectional terminator (BBa_B0014), PBad Weak (BBa_K206001) the sensitivity tuner (BBa_K427005 or BBa_K427006) and GFP reporter (BBa_E0840). These parts are BBa_K427007 for the MuC sensitivity tuner and BBa_K427008 for the MuMor sensitivity tuner. <br />
</p><br />
<p class="blah"><br />
All the characterizations were made in the BW27783 strain of E. coli. This strain was modified to internalize arabinose so that the concentration inside the cell is the same as the concentration outside. Another modification is that BW27783 does not use or degrade arabinose.The graphs show the data used to obtain the coefficients reported in the table. The protocol we used is described in the Protocol section of our wiki.<br />
</p><br />
<br />
<a name="muc"></a> <h4> MuC sensitivity tuner (BBa_K427005)</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/a/a6/Mucgraph.png"><br />
</center><br />
<br><br />
<a name="mumor"></a> <h4>MuMor sensitivity tuner (BBa_K427006)</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0f/Mumorgraph.png"><br />
</center><br />
<br><br />
<a name="transfer"></a> <h4>Transfer function parameters</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/7/72/Transferfunction.png"><br />
</center><br />
<br><br><br />
<br />
<a name="references"></a> <h2>References</h2><br />
<a name="jiang"></a><b> Jiang, Y. a. (2008).</b> Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.<br />
<br><br><br />
<p class="blah"><a name="kumaraswami"></a><b> Kumaraswami, M., & Howe, M. a.-W. (2004).</b> Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.<br />
<br><br><br />
<a name="mathee"></a><b> Mathee, K. a. (1990).</b> Identification of a Positive Regulator of the Mu Middle Operon. Journal of bacteriology, 6641-6650.<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Geneticframe
Team:Tec-Monterrey/Geneticframe
2010-10-27T10:21:19Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/5/55/Geneticframe.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<ol class="menud"><br />
<a href="#development"><li>Development of the genetic frame for an “Intelligent Biosensor”<br />
</a></li><br />
<a href="#phage"><li>Phage Activators</a></li><br />
<ol class="menue"><br />
<a href="#proteins"><li>The Proteins</a></li><br />
<a href="#promoters"><li>The Promoters</a></li><br />
<a href="#sensitivity"><li>The Sensitivity Tuners</a></li><br />
<br />
<a href="#characterization"><li>Characterization</a></li><br />
<a href="#muc"><li>MuC sensitivity tuner (BBa_K427005)</a></li><br />
<a href="#mumor"><li>MuMor sensitivity tuner (BBa_K427006)</a></li><br />
<a href="#transfer"><li>Transfer Function Parameters</a></li><br />
</ol><br />
<a href="#references"><li>References</a></li><br />
<br />
</ol><br />
</p><br />
<br><br />
<h2><a name="development"></a> Development of the genetic frame for an “Intelligent Biosensor”</h2><br />
<p class="blah"><br />
After deciding we wanted to make a concentration sensitive biosensor, we began to investigate the different ways we could achieve our goal. After an initial research concerning biological reporters and the mechanisms currently used to make them, we began to read past iGEM projects to look for useful BioBricks that could be applied to our work. Two past iGEM projects were of our particular interest: British Columbia’s 2009 project and Cambridge’s 2007 project. <br />
</p><br />
<br />
<p class="blah"><br />
<ul><br />
<li><b>British Columbia’s project</b><br />
<p class="blah"><br />
In the past iGEM competition The University of British Columbia team developed a project that resembled our initial proposition. They wanted to produce an <i>E. coli</i> that expressed different Fluorescent Proteins depending on the concentration of arabinose in the medium. Basically, they proposed a system that used three different sensitive promoters to activate a “Lock and Key” mechanism that enabled the expression of the Fluorescent Protein. There were 3 promoters: the wild type pBAD promoter that is sensitive to arabinose, and two others called pBAD weak and pBAD strong that were designed by the team via mutagenesis. As their name implies, the weak promoter is sensitive only to higher concentrations of arabinose and the strong promoter is sensitive to lower concentrations of arabinose compared to the wild type. Finally, to prevent the expression of all the fluorescent proteins when there was enough arabinose in the medium to activate all three promoters, they developed a “jammer” that consisted in placing a reverse promoter on the other side of the fluorescent protein. Thus, when both promoters were activated, the production of fluorescent protein ceased. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="https://2009.igem.org/Team:British_Columbia" target="blank">consult their wiki</a>)</i><br />
</p><br />
<br />
<li><b>Cambridge’s project</b><br />
<p class="blah"><br />
In 2007, the University of Cambridge iGEM team worked in developing both intracellular and intercellular communication pathways as well as a Gram-positive platform for synthetic biology. We were particularly interested in the intracellular project. It consisted of a PoPS amplifier made with transcriptional activators and promoters that were obtained from bacteriophage components. The Cambridge team decided to use activators from bacteriophage components because they are not common in the cellular signals of bacteria. In this particular case, they used activator and promoters from the bacteriophage PSP3 and fR73. <br />
</p><br />
<p class="blah"><br />
<i>(For more information about this project, you can <a href="http://parts.mit.edu/igem07/index.php/Cambridge" target="blank">consult their wiki</a>)</i><br />
</p><br />
</p><br />
</ul><br />
<p class="blah"><br />
After reading about these projects we decided that we could adapt our idea and build upon the achievements of those two previous teams. We wanted to contribute in a significant way, so we began by analyzing their results and identifying points that could use more work. We found that, in general, British Columbia’s proposal was complicated and apparently ineffective. The problem seemed to be in the “lock and key” mechanism, which didn’t seem to behave as expected. On the other hand, we found that their “jammers” could be very useful and they seemed to work as expected.<br />
</p><br />
<br />
<p class="blah"><br />
So we began to design of a different genetic frame to create “intelligent biosensors”, one that preferably didn’t contain a “lock and key” mechanism. Our solution is composed by a combination of parts of the two previously mentioned projects. We incorporated the “jammer” mechanism in addition to PoPS amplification systems in order to create a flexible genetic construction for concentration sensitive bio-reporters. Our proposed solution can be seen in Figure 1. <br />
</p><br />
<br />
<p class="blah"><br />
In theory, this genetic construction is able to detect different concentrations of a compound provided that you have three different promoters that are sensitive to different concentrations of the substance. Based on the amount of compound detected, the bacteria will produce one of three reporter proteins. For our project, we decided to use the same sensors and reporter proteins that the University of British Columbia team used in their 2009 iGEM project. Therefore we began with 3 promoters: pBAD wild type, pBAD weak and pBAD strong; each promoter was able to detect arabinose at different concentrations. Our reporter proteins were GFP, YFP, and RFP (Cherry). We arranged the constructions so that GFP is produced when the cells were induced with low concentrations of arabinose, YFP is produced when induced with medium concentrations and RFP (Cherry) is produced when there is a high concentration. The mechanism that enables this behavior will be explained in two parts, the first part involves the detection of the compound and the second part concerns the production of the reporter protein. <br />
</p><br />
<p class="blah"><br />
The first part of the genetic construction is responsible for the detection of arabinose. This is achieved by using a series of promoters from the pBAD family that were developed and characterized by British Columbia. Each promoter is activated at a different concentration of arabinose, the strong promoter requires a higher concentration of arabinose than the weak promoter. When the promoter is activated, it enables the expression of a bacteriophage activator that works as a PoPS amplifier. The first amplifier we used was the one provided by the Cambrdige 2007 team. However, for our construction to work, we required two additional amplifiers. So we did some more research on bacteriophage activators and constructed two new PoPS amplifier systems in order to finish our construction. We consider these new amplifiers to be a valuable addition to the Registry of Standard Biological Parts, because they come from different bacteriophage families, this means that they produce different activator proteins and they can be used at the same time without causing interference. <br />
</p><br />
<p class="blah"><br />
Eventually we realized that if we didn’t have a mechanism that disabled the expression the PoPS amplifiers, when the cells were induced with high concentrations of arabinose, all three reporter proteins would be produced. Once again, incorporating the previous advances of the 2009 British Columbia Team, we were able to overcome this obstacle. The solution consisted in implementing their “jammer” systems, in order to suppress the expression of the PoPS amplifiers as the concentration of arabinose in the medium increases. The full mechanism is explained in the following figures: <br />
</p><br />
<ul><br />
<li>Figure 2: shows the sensor part of the final construct</li><br />
<li>Figure 3: shows the sensor when there is a low concentration of arabinose</li><br />
<li>Figure 4: shows the sensor when there is a medium concentration of arabinose</li><br />
<li>Figure 5: shows the sensor when there is a high concentration of arabinose</li><br />
</ul><br />
<p class="blah"><br />
The second part of the construction is in charge of producing the reporter proteins. According to our research on biological sensors, the reporter molecule must be easy to detect and it must be a substance that is not naturally produced by the organism. Fluorescent proteins are great reporter proteins because fulfill both of these requirements. The team from British Columbia University used three different fluorescent proteins in their project in the 2009 competition: Green Fluorescent Protein (GFP), Yellow Fluorescent Protein (YFP), and the Cherry variety of the Red Fluorescent Protein (RFP Cherry). We ultimately decided to use the same three molecules because they have been widely characterized and there is plenty of information regarding their fluorescence spectrum and behavior. <br />
</p><br />
<br />
<p class="blah"><br />
When the sensing part of our construction produces one of the bacteriophage activators, this molecule enables the expression of one of the reporter proteins. Because these promoters are produce have very high PoPS numbers, the amount of reporter molecule produced is elevated, even though the arabinose concentration isn’t. The following figure is a detail that explains the mechanism behind the production of reporter proteins. <br />
</p><br />
<p class="blah"><br />
<br />
Figure 6: shows the reporter part of the final construct. <br />
</p><br />
<br />
<br />
<br><br />
<br />
<h2><a name="phage"></a>Phage Activators</h2><br />
<p class="blah"><br />
The most basic bio-bricks created in our project were sequences form the genome of the Mu bacteriophage that attacks E. coli. We needed some sort of sensitivity tuner for our biosensor and the registry had only one family of promoters and protein activators that could be used for the construction of these devices. After some research we came to Mu bacteriophage, where we found two different families of promoters and protein activators: Mor protein – Pm promoter and C protein - Plys, Pi, Pp and Pmom promoters. We took the sequences reported sequences of Mor, Pm, C <a href="#kumaraswami">(Kumaraswami & Howe, 2004)</a> and Pmom <a href="#jiang">(Jiang, 2008)</a>. The proteins’ sequences were optimized for their expression in E. coli with Mr. Gene’s optimization algorithm; we added an Elowitzribosome binding site and finally the BioBrick prefix and suffix to create the bio-bricks that we used. We only added the prefix and suffix to the promoters’ sequences.<br />
</p><br />
<a name="proteins"></a><h4>The Proteins</h4><br />
<p class="blah"><br />
Mor and C are both proteins encoded in the genome of Mu bacteriophage, and they have similar characteristics. Both are proteins bind to DNA in a specific sequence, both have about the same size, sequence and have similar secondary structures and domains. The next image <a href="#mathee">(Mathee, 1990)</a> shows the amino acid sequence alignment of Mor and C proteins.<br />
</p><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/81/Morandc.png"><br />
</center><br />
<br />
<p class="blah"><br />
In that image we can see the similarity between these two proteins, but we can also notice that the helix turn helix motifs are quite different, it is hypothesized that this domains are the ones that recognize and interact with the DNA sequence. According to <a href="#kumaraswami">Kumaraswami (2004)</a> and <a href="#jiang">Jiang (2008)</a> two domains can be observed on each of the proteins a Helix-Turn-Helix (HTH) domain and a dimerization domain, which suggests that the proteins can only activate their promoters when in dimers.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Mor (BBa_K427002) and C (BBa_K427001)<br />
</p><br />
<br />
<a name="promoters"></a><h4>The Promoters</h4><br />
<p class="blah"><br />
The first family of activators that we used was the Mor-Pm family. Pm promoter is the middle promoter of the Mu bacteriophage, where it initiates the transcription of C protein. The second family had four promoters: Plys, Pi, Pp and Pmom. We chose Pmom for our sensitivity tuners because out of the four promoters it is the most characterized in literature. It is normally activated by the C protein.<br />
</p><br />
<p class="blah"><br />
The activation of these promoters requires the holenzymeσ^70, which normally binds to the -35 and -10 consensus sequences, these promoters have the -10 but do not have the -35 sequences instead they have another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzymeσ^70 recognizes the promoter and transcription begins.<br />
</p><br />
<p class="blah"><br />
The final bio-bricks were: Pm (BBa_K427003), Pmom (BBa_K427004)<br />
</p><br />
<br />
<a name="sensitivity"></a> <h4>The Sensitivity Tuners</h4><br />
<p class="blah"><br />
These devices were designed with two purposes in mind, increase the PoPS output of the pBad promoter (any promoter would work) and change its almost linear production into an almost Boolean one. These parts have the following structure: activator protein, transcriptional stop and phage promoter. The sensitivity tuners can be used to achieve both purposes on any construction they just have to be inserted between the desired promoter and the desired coding sequence.<br />
</p><br />
<p class="blah"><br />
In our project we used three sensitivity tuners, one made by Cambridge in previous iGEMcompetitions;this one is built with phiR73 delta activator (BBa_I746352), a bidirectional transcriptional terminator (BBa_B0014) and Po phage promoter (BBa_I746361). The other two were built by us using the Mor and C families. They are MuC sensitivity tuner (BBa_K427005) and MuMor sensitivity tuner (BBa_K427006). The first one includes MuC protein activator (BBa_K427002) bidirectional terminator (BBa_B0014) and Pmom promoter (BBA_K427004). The other includes MuMor protein activator (BBa_K427001) bidirectional terminator (BBa_B0014) and Pmom promoter (BBA_K427003).<br />
</p><br />
<p class="blah"><br />
MuC sensitivity tuner (BBa_K427005)<br />
<br>IMAGEN<br />
</p><br />
<br />
<p class="blah"><br />
MuMor sensitivity tuner (BBa_K427006)<br />
<br>IMAGEN<br />
</p><br />
<br />
<a name="characterization"></a> <h4>Characterization</h4><br />
<p class="blah"><br />
We characterized both sensitivity tuners using Hill equations:</p><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/4/4d/Hilleq.png"><br />
</center><br />
<p class="blah"><br />
In order to do this we had to build another two parts, they were used only to characterize the sensitivity tuners they have a bidirectional terminator (BBa_B0014), PBad Weak (BBa_K206001) the sensitivity tuner (BBa_K427005 or BBa_K427006) and GFP reporter (BBa_E0840). These parts are BBa_K427007 for the MuC sensitivity tuner and BBa_K427008 for the MuMor sensitivity tuner. <br />
</p><br />
<p class="blah"><br />
All the characterizations were made in the BW27783 strain of E. coli. This strain was modified to internalize arabinose so that the concentration inside the cell is the same as the concentration outside. Another modification is that BW27783 does not use or degrade arabinose.The graphs show the data used to obtain the coefficients reported in the table. The protocol we used is described in the Protocol section of our wiki.<br />
</p><br />
<br />
<a name="muc"></a> <h4> MuC sensitivity tuner (BBa_K427005)</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/a/a6/Mucgraph.png"><br />
</center><br />
<br><br />
<a name="mumor"></a> <h4>MuMor sensitivity tuner (BBa_K427006)</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0f/Mumorgraph.png"><br />
</center><br />
<br><br />
<a name="transfer"></a> <h4>Transfer function parameters</h4><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/7/72/Transferfunction.png"><br />
</center><br />
<br><br><br />
<br />
<a name="references"></a> <h2>References</h2><br />
<a name="jiang"></a><b> Jiang, Y. a. (2008).</b> Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.<br />
<br><br><br />
<p class="blah"><a name="kumaraswami"></a><b> Kumaraswami, M., & Howe, M. a.-W. (2004).</b> Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.<br />
<br><br><br />
<a name="mathee"></a><b> Mathee, K. a. (1990).</b> Identification of a Positive Regulator of the Mu Middle Operon. Journal of bacteriology, 6641-6650.<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Instructors
Team:Tec-Monterrey/Instructors
2010-10-27T09:38:40Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/a/a8/Instructors.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
</p><br />
<div class="footercon"><br />
<h2>Dr. Mario Moisés Álvarez</h2><br />
<p class="blahimgizq"><br />
<img src="https://static.igem.org/mediawiki/2010/9/9b/Drmario.jpg" alt="Dr. Mario Álvarez" /><br />
Mario Moisés Álvarez is the former director of the FEMSA Biotechnology Center at the Tecnológico de Monterrey in México. He is the coordinator of the Biotechnology Graduate Program in this same institution, and one of the youngest scientists in México ranked as a Level 2 Researcher by the Mexican System of Researchers (SNI). Dr. Álvarez has a Ph.D. from Rutgers University in Chemical and Biochemical Engineering, a Masters in Science in Chemical Engineering from Rutgers University, and a Masters in Science in Process Engineering and a Bachelor Degree in Biochemistry from the Tecnológico de Monterrey. He did a Postdoctoral stay at Bristol Myers Squibb, at the Pharmaceutical Research Institute, where he collaborated in different projects related to the design and scale-up of Pharmaceutical Processes, particularly reactive and crystallization processes. <br />
</p><br />
<p class="blah"><br />
Dr. Álvarez's main area of scientific expertise is laminar mixing (mixing operations at low speed or in high viscosity conditions). Currently, his group is working in the application of mixing knowledge on the design of bioreactors. This includes the design of novel high performance bioreactor systems for animal cell culture, the diagnose of mixing performance in bioreactors through reactive methods, and the study of non-ideal mixing conditions in bioreactive systems.<br />
</p><br />
</div><br />
<div class="footercon"><br />
<h2>M. Sc. Sergio Ángel García Echauri</h2><br />
<p class="blahimgizq"><br />
<img src="https://static.igem.org/mediawiki/2010/a/a6/Sergio.jpg" alt="M. Sc. Sergio García" /><br />
Sergio Ángel García Echauri is a researcher in the FEMSA Biotechnology Center at the Tecnológico de Monterrey in México, where he is currently working in his Ph.D. His early formation was in the Autonomous University of Baja California, where he obtained his title as a Biologist. He then carried out a thesis on the Isolation and Identification of Psychrophillic Organisms from the Antarctica and Expression of a Superoxide Dismutase from <i>Deschampsia antarctica</i> in <i>Escherichia coli</i>.<br />
</p><br />
<p class="blah"><br />
During the influenza outbreak in 2009, Sergio was part of the team that developed a novel technique for its detection and the creation of a recombinant vaccine against the A/H1N1. He was then invited to the Novartis Fourth Annual Global BioCamp, an event for the biotechnology leaders from the best universities in 27 countries, held at Cambridge, Massachusets in October 2009. <br />
<br />
</p><br />
</div><br />
</div><br />
<div class="ft"><div class="c"> </div></div> <br />
<div class="bgr"><br><br />
</div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f5/Advisorstitle.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
</p><br />
<div class="footercon"><br />
<h2>Dr. Manuel I. Zertuche Guerra</h2><br />
<p class="blahimgizq"><br />
<img src="https://static.igem.org/mediawiki/2010/4/42/Drzertuche.jpg" alt="Dr. Manuel Zertuche" /><br />
Dr. Manuel Zertuche Guerra has a Masters Degree in Science with a specialization in Production Systems and a Ph.D. in Plant Physiology from Texas A&M. In 1992, he was named the Head of the International Center for Agribusinesses and then became the Director of the Center of Strategic Studies in the Tecnológico de Monterrey. He developed strategic planning projects in Venezuela, Ecuador, Argentina, Bolivia, Nicaragua, and El Salvador. He works as a consultant and has participated in several conferences and seminars in the United States and Canada. He is currently the Head of the Biotechnology and Food Division in the Tecnológico de Monterrey and leads several research projects in collaboration with important Mexican corporations, such as FEMSA, CEMEX, LALA, Tyson, and Landsteiner Scientific. <br />
</p><br />
</div><br />
<div class="footercon"><br />
<h2>M. Sc. José Manuel Aguilar Yáñez</h2><br />
<p class="blahimgizq"><br />
<img src="https://static.igem.org/mediawiki/2010/d/d6/Chema.jpg" alt="M. Sc. José Manuel Aguilar" /><br />
José Manuel Aguilar majored in Biology with a specialty in Molecular Biology at the Baja California Autonomous University. In 2007, he got a M.S. Degree in Biotechnology at the Tecnológico de Monterrey, where he was awarded an Honorary Mention for Excellence and an Outstanding Thesis Mention. He has two scientific publications and two more under revision, as well as five national patent requests and one Patent Cooperation Treaty. As of now, he works at the Tecnológico de Monterrey as a professor and is on his way to finish his Ph.D.<br />
<br />
</p><br />
</div><br />
<div class="footercon"><br />
<h2>M. Sc. Johari Salgado Gallegos</h2><br />
<p class="blahimgizq"><br />
<img src="https://static.igem.org/mediawiki/2010/7/71/Johari.jpg" alt="M. Sc. Johari Salgado" /><br />
Johari Salgado is a biologist graduated from the Baja California Autonomous University. In 2007, he received an M.S. Degree in Biotechnology at the Tecnológico de Monterrey. He participated in the development of an Influenza vaccine during the A/H1N1 2009 outbreak and currently leads several projects in collaboration with Landsteiner Scientific, in addition to teaching the Genetic Engineering Laboratory in the Tecnológico de Monterrey. <br />
</p><br />
</div><br />
<div class="footercon"><br />
<h2>Felipe López Pacheco</h2><br />
<p class="blahimgizq"><br />
<img src="https://static.igem.org/mediawiki/igem.org/f/fe/Felipin.jpg" alt="Felipe López" /><br />
Felipe López Pacheco has a technical degree in Biotechnology and is currently majoring in Biotechnology Engineering. As part of a joint project between the Landsteiner Pharmaceutical Group and ITESM, he is in charge of performing ELISAS to find antibodies.<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
</p><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"> </div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Attributions
Team:Tec-Monterrey/Attributions
2010-10-27T09:34:32Z
<p>Andreshuerta: New page: <html> <head> <meta http-equiv="content-type" content="text/html;charset=utf-8" /> <title>Tec de Monterrey </title> <style> #search-controls { display: none; } </style> </head> ...</p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/8d/Attributions.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<ol class="menud"><br />
<a href="#undergrads"><li>Undergraduates</a></li><br />
<a href="#instructors"><li>Instructors</a></li><br />
<a href="#advisors"><li>Advisors</a></li><br />
<a href="#igemteams"><li>Other iGEM Teams</a></li><br />
</ol><br />
</p><br />
<br><br />
<h2><a name="undergrads"></a>Undergraduates</h2><br />
<h4>José Argüelles</h4><br />
<p class="blah"><br />
He investigated theoretical information for the project, made general laboratory work and made the synthesis design. He was committed to finish the final construct.<br />
</p> <br />
<h4>Pamela Campos</h4><br />
<p class="blah"><br />
She was in charge of managing the material at the laboratory. She helped with general lab work. She also contributed by writing the <a href= "https://static.igem.org/mediawiki/igem.org/4/48/Importation_manual.pdf" target="blank">Importationg Manual</a> and by going to the government offices to request information about the permits we needed in order to import our DNA synthesis.<br />
</p><br />
<br />
<h4>Cristóbal Cevallos</h4><br />
<p class="blah"><br />
He looked for sponsors, made a fund rising party and was in charge of the trip logistics to Boston. He worked on general laboratory work and with the characterizations of pBAD, MuC and MuMor.<br />
<br />
</p><br />
<br />
<h4>Jan Contreras</h4><br />
<p class="blah"><br />
He helped with general laboratory work, contributed in the design of the final construct and the sensitivity tuner. He did the mathematical modelling and characterization of pBAD, MuC and MuMor sensitivity tuners and participated also in the registration of the BioBricks.<br />
<br />
</p><br />
<br />
<h4>Héctor Gálvez</h4><br />
<p class="blah"><br />
He took the leadership of the group and helped to organize the work to be done by defining specific roles, so that every team member would take responsibility on its own task. He also contributed with the logistic of the project and made the calendar in order to achieve all our goals. The project summary was written by him and he also organized all the information and documentation for the wiki. During the summer he helped in general lab work and contacted the government for sponsorship.<br />
<br />
</p><br />
<br />
<h4>Andrés Huerta</h4><br />
<p class="blah"><br />
He was in charge of the design and codification of the team wiki, the T-shirts and the poster. He made general laboratory work and helped in the DNA importation process.<br />
<br />
</p><br />
<br />
<h4>Tatiana Núñez</h4><br />
<p class="blah"><br />
She was in charge of the administration of chemical reactants, general laboratory work, and customs information of the DNA importation. She did the registration and submission of DNA Biobricks.<br />
<br />
</p><br />
<br />
<h4>Diana Ostos</h4><br />
<p class="blah"><br />
Diana contributed with the logistics on the project related with the university and also facilitated the synthesis payment from some parts we need to complete our project. She worked at general laboratory work and helped with the customs procedures. She worked on the development of the sensitivity tuner of Mu bacteriophage with protein C and the design of the final construct.<br />
<br />
</p><br />
<br />
<h4>Javier Reynoso</h4><br />
<p class="blah"><br />
He looked for sponsors and helped with general laboratory work.<br />
<br />
</p><br />
<br />
<h4>Flavio Siller</h4><br />
<p class="blah"><br />
He helped with general lab work. He designed a program to calculate the amount of enzymes for our digestions and also for the ligation reactions.<br />
<br />
</p><br />
<br />
<h2><a name="instructors"></a>Instructors</h2><br />
<h4>Dr. Mario Álvarez</h4><br />
<p class="blah"><br />
He was the first one to believe in the project and to give us support by suggesting ideas for different applications for the sensor.<br />
<br />
</p> <br />
<h4>M. Sc. Sergio García</h4><br />
<p class="blah"><br />
He helped us by teaching the protocols and procedures we needed in the laboratory practices. He contributed by giving us different perspectives in troubleshooting. Sergio always supported us with lab material and disposition of equipment and was incredibly patient with us.<br />
</p><br />
<br />
<h2><a name="advisors"></a>Advisors</h2><br />
<h4>Dr. Manuel Zertuche</h4><br />
<p class="blah"><br />
He was the one who principally financiered and helped us with the organization plan and the timeline in order to achieve the short and long term goals in this project. He provided us with the facilities and gave us his approval to almost everything we asked for. We really want to give special thanks to him because of the advices and support that he gave us along this time, but above all for having believed in us.<br />
<br />
</p> <br />
<br />
<h4>M. Sc. José Manuel Aguilar</h4><br />
<p class="blah"><br />
He helped us with the theoretical information and developed with us the idea of the intelligent biosensor<br />
<br />
</p><br />
<br />
<h4>M. Sc. Johari Salgado</h4><br />
<p class="blah"><br />
He shared with us the laboratory and provided us with material and equipment for the laboratory practices.<br />
<br />
</p><br />
<br />
<h4>Felipe López</h4><br />
<p class="blah"><br />
He instructed us on how to use the microplate reader for the characterization of our parts.<br />
</p><br />
<br />
<h2><a name="igemteams"></a>Other iGEM Teams</h2><br />
<p class="blah"><br />
We contributed with other universities by participating on the surveys they sent to us; unfortunately we couldn’t answer to all of them, but we tried to have an active contribution in order to help them in their duty.<br />
<br />
</p> <br />
<br />
<h4>TU Delft</h4><br />
<p class="blah"><br />
We didn’t have a proper collaboration with them, but we took part of their code from their Wiki, so we would like to thank them!<br />
<br />
</p> <br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Thanks
Team:Tec-Monterrey/Thanks
2010-10-27T09:10:24Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
.imgcenter img {border:1px solid #05182b; margin:5px 5px 5px 5px;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/5/5a/Specialthanks.png" border="0" ><br />
<div class="bdy"><br />
<p class="blah"><br />
</p> <br />
<div class="footercon"><br />
<p class="blahimgizq"><br />
All of these people hold a very special place in the iGEM Tec de Monterrey Team. Without their help in this project, things wouldn’t have come out the way they did, so here’s to them!<br />
</p><br />
<ul ><br />
<li>Dr. Marco Rito Palomares</li><br />
<li>Dr. Guy A. Cardineau</li><br />
<li>Dr. Martín Hernández</li><br />
<li>M.Sc. Juan Gerardo Cantú</li><br />
<li>Lic. Myriam Robledo </li><br />
<li>Lic. Belinda Vargas Heredia</li><br />
<li>C.P. Claudia Dávila Ramos</li><br />
<li>Dra. Bertha de la Re</li><br />
<li>M.Sc. Yuriana Oropeza Almazán</li><br />
<li>Ing. Gabriela González Romero</li><br />
<li>Lic. Pamela Sánchez</li><br />
<li>Lic. Priscilla García</li><br />
<li>C.P. Laura M. Suárez</li><br />
<li>C.P. Mario González</li><br />
<li>Elsita Salas Cabello</li><br />
<li>Sra. Ethel Peniche Arjona</li><br />
<li>Biol. Ramón E. Rivero</li><br />
<li>Lic. Martha Carrillo</li><br />
<li>Ing. Flora Piña</li><br />
<li>Carlos</li><br />
<li>All of our biotech friends</li><br />
<li>Our families</li><br />
</ul><br />
<p class="blah"><br />
To all of you, our most sincere gratitude again.<br />
</p><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/0/08/Thankyou.jpg"><br />
</center><br />
</div><br />
<br />
<br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Silver
Team:Tec-Monterrey/Silver
2010-10-27T09:09:36Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:965px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
<br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/0/0b/Silver.png"><br />
<div class="bdy"><br />
<p class="blah"><br />
</p><br />
<div class="footercon"><br />
<h2>Autodesk</h2><br />
<p class="blahimgizq"><br />
<a href="http://usa.autodesk.com/" target="blank"><img src="http://upload.wikimedia.org/wikipedia/commons/thumb/9/96/Autodesk_logo.svg/200px-Autodesk_logo.svg.png"></a><br />
Autodesk is a world leader in 3D design, engineering, and entertainment software.<br />
<br />
</p><br />
</div><br />
<div class="footercon"><br />
<h2>Laura Garza</h2><br />
<p class="blahimgizq"><br />
<a href="http://www.lauragarza.com" target="blank"><img src="https://static.igem.org/mediawiki/2010/7/76/Lauragarza.jpg"></a><br />
<p class="blah"><br />
Laura Garza is a Mexican photographer passionate about creating real emotions in her images. <br><br><br><br />
<br />
</p><br />
</div><br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Gold
Team:Tec-Monterrey/Gold
2010-10-27T09:08:45Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:965px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/1/10/Gold.png"><br />
<div class="bdy"><br />
<p class="blah"><br />
</p><br />
<div class="footercon"><br />
<h2>Millipore</h2><br />
<p class="blahimgizq"><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/0/07/MilliporeLogo.jpg" width="250px" height="186px"></a><br />
Millipore is a Life Science leader providing cutting-edge technologies, tools, and services for bioscience research and biopharmaceutical manufacturing. As a strategic partner, we collaborate with customers to confront the world's challenging human health issues. From research through development to production, our scientific expertise and innovative solutions help customers tackle their most complex problems and achieve their goals.<br />
<br><br><br><br><br><br><br />
</p><br />
</div><br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Platinum
Team:Tec-Monterrey/Platinum
2010-10-27T09:08:01Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:965px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {position:relative;<br />
left:20px;;}<br />
</style><br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li><br />
</ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/2010/a/ac/Platinum.png"><br />
<div class="bdy"><br />
<p class="blah"><br />
</p><br />
<div class="footercon"><br />
<h2>Uniparts</h2><br />
<p class="blahimgder"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/db/Uniparts2.jpg"></a><br />
Uniparts is a company with 18 years of experience in the distribution of high quality reactants, materials and equipment for research and diagnosis, directed to the scientific and clinical community. <br><br><br><br><br><br><br />
</p><br />
</div><br />
<div class="footercon"><br />
<h2>Promega</h2><br />
<p class="blahimgder"><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/34/Promega2.jpg"></a><br />
<p class="blah"><br />
Promega is a leader in providing innovative solutions and technical support to the life sciences industry. Promega Corporation’s 2,000 products enable scientists worldwide to advance their knowledge in life science research, particularly in genomics, proteomics, and cellular analysis. Founded in 1978, Promega Corporation has branches in 14 countries and over 50 global distributors.</p><br><br><br><br><br />
</div><br />
<div class="footercon"><br />
<h2>Corning</h2><br />
<p class="blahimgder"><br />
<a href="http://www.corning.com" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/22/Corning2.jpg"></a><br />
<p class="blah"><br />
Corning Incorporated is the world leader in specialty glass and ceramics. Drawing on more than 150 years of materials science and process engineering knowledge, Corning creates and makes keystone components that enable high-technology systems for consumer electronics, mobile emissions control, telecommunications and life sciences. <br><br><br><br><br><br><br />
</p><br />
</div><br />
<div class="footercon"><br />
<h2>Sartorius</h2><br />
<p class="blahimgder"><br />
<a href="http://www.sartorius.com" target="blank"><img src="https://static.igem.org/mediawiki/2010/c/c5/Sartorius.jpg"></a><br />
<p class="blah"><br />
Sartorius is an internationally leading laboratory and process technology provider covering the segments of biotechnology and mechatronics. Strongly rooted in the scientific community and closely allied with technology partners, Sartorius is dedicated to its philosophy of turning science into solutions.. <br><br><br><br><br><br><br />
</p><br />
</div><br />
<br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
</div><br />
<br />
</html></div>
Andreshuerta
http://2010.igem.org/Team:Tec-Monterrey/Humanpractices
Team:Tec-Monterrey/Humanpractices
2010-10-27T09:07:11Z
<p>Andreshuerta: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html;charset=utf-8" /><br />
<title>Tec de Monterrey </title> <br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style><br />
</head><br />
<br />
<style><br />
html, body {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
body {<br />
background-color: #05182b;<br />
background-position: top center;<br />
}<br />
<br />
/* Wiki Hacks - START */<br />
<br />
<br />
<br />
#globalWrapper {<br />
background-color: transparent;<br />
border: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#content {<br />
background-color: transparent;<br />
border: none;<br />
padding: 0;<br />
margin: 0;<br />
width: 100%;<br />
}<br />
<br />
#bodyContent {<br />
border: none;<br />
padding:0;<br />
margin:0;<br />
width:100%;<br />
}<br />
<br />
#top-section {<br />
height: 20px;<br />
margin-top: -5px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 10px;<br />
padding:0;<br />
border: none;<br />
}<br />
<br />
#p-logo {<br />
height:1px;<br />
overflow:hidden;<br />
display: none;<br />
}<br />
<br />
#search-controls {<br />
overflow:hidden;<br />
display:block;<br />
background: none;<br />
position: absolute;<br />
top: 100px;<br />
right: 40px;<br />
}<br />
<br />
.left-menu {<br />
width: 400px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.left-menu ul {<br />
border: none;<br />
}<br />
<br />
#menubar.right-menu {<br />
width:300px;<br />
display:block;<br />
float:left;<br />
margin-top:-80px;<br />
border: none;<br />
}<br />
<br />
.right-menu ul {<br />
border: none;<br />
}<br />
<br />
#footer-box {<br />
background-color: transparent;<br />
border: none;<br />
width: 965px;<br />
margin: 0 auto;<br />
padding: 0;<br />
}<br />
<br />
#footer {<br />
border: none;<br />
width: 965px;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.firstHeading {<br />
display: none;<br />
}<br />
<br />
#f-list a {<br />
color: #ffffff;<br />
font-size: 10px;<br />
}<br />
<br />
#f-list a:hover {<br />
color: #666;<br />
}<br />
<br />
#footer ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer ul li {<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 10px;<br />
margin-right: 10px;<br />
padding: 0;<br />
}<br />
<br />
/* Wiki Hacks - END */<br />
<br />
#wrapper {<br />
width: 965px;<br />
margin: 0 auto;<br />
}<br />
<br />
#header_wrap {<br />
width:100%;<br />
height:107px;<br />
}<br />
<br />
#menu_wrap {<br />
width:100%;<br />
height:45px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#content_wrap {<br />
width:100%;<br />
/* background-color: #fff; */<br />
margin-top: -24px;<br />
display:block;<br />
float: left;<br />
}<br />
<br />
#footer_wrap {<br />
width:100%;<br />
height:40px;<br />
background-color: transparent;<br />
display:block;<br />
float: left;<br />
padding-top: 10px;<br />
}<br />
<br />
/* Footer START */<br />
<br />
#footer_wrap #copyright {<br />
display: block;<br />
float: left;<br />
width: 300px;<br />
text-align: center;<br />
font-size: 10px;<br />
color: #ffffff;<br />
}<br />
<br />
#footer_wrap #footer_links {<br />
display: block;<br />
float: right;<br />
width: 500px;<br />
text-align: center;<br />
font-size: 10px;<br />
}<br />
<br />
#footer_links ul {<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
#footer_links ul li {<br />
display: block;<br />
list-style-type: none;<br />
float:left;<br />
padding: 0;<br />
margin: 0 5px 0 5px;<br />
}<br />
<br />
#footer_links ul li a {<br />
color: #ccc;<br />
text-decoration: none;<br />
}<br />
<br />
#footer_links ul li a:hover {<br />
text-decoration: underline;<br />
}<br />
<br />
/* Footer END */<br />
<br />
<br />
<br />
<br />
#panel_mask { <br />
position:absolute;<br />
top:0px;<br />
width: 1000px;<br />
overflow:hidden; <br />
margin:0 auto;<br />
background-color: #05182b;<br />
}<br />
.heading{<br />
color: #000;<br />
font: 14px century gothic;<br />
padding: 5px 5px 2px 5px;<br />
font-weight: bold;<br />
}<br />
<br />
.header{<br />
z-index: 1;<br />
position: absolute;<br />
top: 5px;<br />
height: 300px;<br />
}<br />
<br />
.menu{<br />
z-index: 5;<br />
position: absolute;<br />
top: 188px ;<br />
font: 18px Arial;<br />
text-align: center;<br />
}<br />
<br />
<br />
.menubg{<br />
position: absolute;<br />
top: 220px;<br />
background-color: #FFFFFF;<br />
width: 966px;<br />
height: 35px;<br />
/* for IE */<br />
filter:alpha(opacity=75);<br />
/* CSS3 standard */<br />
opacity:0.75;<br />
z-index:2;<br />
}<br />
a:link{color: #fff;}<br />
a:visited{color: #fff;}<br />
a:active{color: #fff;}<br />
<br />
.menubg .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
/* for IE */<br />
filter:alpha(opacity=100);<br />
/* CSS3 standard */<br />
opacity:1;<br />
}<br />
<br />
.menubg .ft .c {<br />
height:35px;<br />
}<br />
.menubg .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
<br />
}<br />
<br />
.menubg .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.righ{<br />
float:right;<br />
width:220px;<br />
background-color:#FFFFFF;<br />
margin:5px 0px 10px 5px;<br />
}<br />
.righ .bdy {<br />
padding:0px 10px 0px 15px;<br />
}<br />
.righ .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.righ .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.righ .hd .g,<br />
.righ .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
<br />
.righ .ft .c {<br />
height:14px;<br />
}<br />
.righ .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.righ .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
<br />
.righ .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
<br />
<br />
.bbody{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:965px;<br />
<br />
<br />
}<br />
.texteando{<br />
float:left;<br />
width:715px;<br />
background-color:#ffffff;<br />
margin:5px 5px 10px 0px;<br />
}<br />
.texteando .bdy {<br />
padding:0px 15px 0px 15px;<br />
}<br />
.texteando .top {<br />
padding:0px 0px 0px 0px;<br />
}<br />
.texteando .bgr{<br />
background-color:#05182b;<br />
height:15px;<br />
}<br />
.texteando .hd .g,<br />
.texteando .ft .c {<br />
font-size:1px; /* ensure minimum height */<br />
height:13px;<br />
}<br />
.texteando .ft .c {<br />
height:14px;<br />
}<br />
.texteando .ft {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .ft .c {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;<br />
}<br />
.texteando .hd {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;<br />
}<br />
.texteando .hd .g {<br />
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;<br />
}<br />
.bbody2{<br />
margin-left:auto;<br />
margin-right:auto;<br />
width:715px;<br />
}<br />
.texteando .headercon<br />
{<br />
padding:0.5em;<br />
color:white;<br />
background-color:gray;<br />
clear:left;<br />
}<br />
.texteando .footercon<br />
{<br />
padding:0.5em;<br />
background-color:white;<br />
clear:left;<br />
}<br />
.texteando .leftcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
padding:1em;<br />
}<br />
.texteando .rightcon<br />
{<br />
float:left;<br />
width:212px;<br />
margin:0;<br />
border-left:1px solid gray;<br />
padding:1em;<br />
}<br />
</style><br />
<br />
<body bgcolor="#414141"><br />
<br />
<style><br />
#search-controls {<br />
display: none;<br />
}<br />
</style> <br />
<br />
<style type="text/css"><br />
.blah {text-align:justify}<br />
.blahimgder {text-align:justify}<br />
.blahimgizq {text-align:justify;}<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista {text-align:justify}<br />
ol.listad {text-align:justify}<br />
</style><br />
<br />
<style type="text/css"><br />
.blah A:link {text-decoration: none; color: #0D3C6A }<br />
.blah A:visited {text-decoration: none; color: #0D3C6A}<br />
.blah A:active {text-decoration: none;color: #0D3C6A}<br />
.blah A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
.blahimgder A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgder A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgder A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgder A:hover {text-decoration: underline; color: #186FC5;} <br />
.blahimgizq A:link {text-decoration: none; color: #0D3C6A }<br />
.blahimgizq A:visited {text-decoration: none; color: #0D3C6A}<br />
.blahimgizq A:active {text-decoration: none;color: #0D3C6A}<br />
.blahimgizq A:hover {text-decoration: underline; color: #186FC5;}<br />
<br />
</style><br />
<style type="text/css"><br />
.blahimgder img {float:right; border:1px solid #05182b; margin:0px 0px 15px 20px}<br />
.blahimgizq img {float:left; border:1px solid #05182b; margin:0px 20px 15px 0px;}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ul.lista A:link {text-decoration: none; color: #0D3C6A }<br />
ul.lista A:visited {text-decoration: none; color: #0D3C6A}<br />
ul.lista A:active {text-decoration: none;color: #0D3C6A}<br />
ul.lista A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.listad A:link {text-decoration: none; color: #0D3C6A }<br />
ol.listad A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.listad A:active {text-decoration: none;color: #0D3C6A}<br />
ol.listad A:hover {text-decoration: underline; color: #186FC5;}<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menud A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menud A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menud A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menud A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menud {text-align:justify}<br />
<br />
</style><br />
<br />
<style type="text/css"><br />
ol.menue A:link {text-decoration: none; color: #0D3C6A }<br />
ol.menue A:visited {text-decoration: none; color: #0D3C6A}<br />
ol.menue A:active {text-decoration: none;color: #0D3C6A}<br />
ol.menue A:hover {text-decoration: underline; color: #186FC5;}<br />
ol.menue {position:relative;<br />
left:30px;}<br />
ol.menue {list-style:lower-roman;}<br />
</style><br />
<br />
<br />
<style type="text/css"><br />
h2 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h3 {font:17px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
h4 {font:14px arial,sans-serif; font-weight:bold; color:#0D3C6A;}<br />
</style><br />
<br />
<style type="text/css"><br />
b {color:#0D3C6A;}<br />
</style><br />
<br />
<br />
<br />
<div class="bbody"><br />
<script type="text/javascript"><!-- <br />
var delay=7000<br />
var ie4=document.all<br />
var curindex=0<br />
var totalcontent=0<br />
function get_total(){<br />
if (ie4){<br />
while (eval("document.all.content"+totalcontent))<br />
totalcontent++<br />
}<br />
else{<br />
while (document.getElementById("content"+totalcontent))<br />
totalcontent++<br />
}<br />
}<br />
function contract_all(){<br />
for (y=0;y<totalcontent;y++){<br />
if (ie4)<br />
eval("document.all.content"+y).style.display="none"<br />
else<br />
document.getElementById("content"+y).style.display="none"<br />
}<br />
}<br />
function expand_one(which){<br />
contract_all()<br />
if (ie4)<br />
eval("document.all.content"+which).style.display=""<br />
else<br />
document.getElementById("content"+which).style.display=""<br />
}<br />
function rotate_content(){<br />
get_total()<br />
contract_all()<br />
expand_one(curindex)<br />
curindex=(curindex<totalcontent-1)? curindex+1: 0<br />
setTimeout("rotate_content()",delay)<br />
}<br />
window.onload=rotate_content<br />
//--></script><br />
<br />
<br />
<div class="header"><br />
<br />
<div id="content0" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/4/4b/Cbplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content1" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/b/b1/Electroplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content2" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/e/ea/Teamplano.png" width="966px" height="250px" border="0"></div><br />
<div id="content3" style="display:none"><br />
<img src="https://static.igem.org/mediawiki/2010/c/cf/Lego_dnaplano.png" width="966px" height="250px" border="0"></div><br />
<br />
</div><br />
<br />
<div class="menubg"><br />
<br><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="menu"><br />
<br />
<!--[if lt IE 7]><br />
<script type="text/javascript"><br />
<br />
sfHover = function() {<br />
var sfEls = document.getElementById("nav").getElementsByTagName("LI");<br />
for (var i=0; i<sfEls.length; i++) {<br />
sfEls[i].onmouseover=function() {<br />
this.className+=" over";<br />
}<br />
sfEls[i].onmouseout=function() {<br />
this.className=this.className.replace(new RegExp(" over\\b"), "");<br />
}<br />
}<br />
}<br />
if (window.attachEvent) window.attachEvent("onload", sfHover);<br />
<br />
</script><br />
<![endif]--><br />
<style type="text/css"><br />
/* general styling */<br />
*{margin:0;padding:0}<br />
body {<br />
font: x-small Verdana, Arial, Helvetica, sans-serif;<br />
text-align:left;<br />
padding:20px;<br />
}<br />
.outer{<br />
padding:0px 0 0 0;<br />
width:966px;<br />
margin:auto;<br />
position:relative;<br />
text-align:center;<br />
}<br />
<br />
<br />
/* drop up */<br />
ul#nav,ul#nav ul {<br />
margin: 0;<br />
padding: 0;<br />
list-style: none;<br />
}<br />
ul#nav {<br />
width:966px;<br />
height:25px;<br />
<br />
}<br />
<br />
ul#nav li {<br />
position: relative;<br />
float: left;<br />
width:161px;<br />
height:25px;<br />
color:#fff;<br />
text-align:left;<br />
}<br />
ul#nav li a {<br />
text-decoration: none;<br />
color:#003366;<br />
<br />
}<br />
#nav li ul {<br />
position: absolute;<br />
left: 0; <br />
top:-105px;<br />
margin-left:-999em;<br />
border:none;<br />
height:137px;<br />
width:161px;<br />
background:#ffffff;<br />
/*opacity for IE5+*/<br />
filter: alpha(opacity=75);<br />
/*opacity for older Mozilla browsers*/<br />
-moz-opacity: 0.75;<br />
/*opacity for mozilla/safari*/<br />
opacity: 0.75;<br />
}<br />
ul#nav li li{height:auto;border:none;}<br />
ul#nav li li a{<br />
color:#fff;<br />
padding:2px 5px 2px 5px;<br />
display:block;<br />
}<br />
ul#nav li:hover ul, <br />
ul#nav li.over ul {<br />
margin-left:0;<br />
}<br />
ul#nav li:hover,<br />
ul#nav li.over,<br />
ul#nav li:hover a,<br />
ul#nav li.over a {color:#000}<br />
<br />
ul#nav li:hover li a,ul#nav li.over li a{color:#003366;} <br />
ul#nav li li a:hover{color:#ffffff; background-color: #05182B;} <br />
ul#nav li p {padding:0px;}<br />
ul#nav li.last,ul#nav li.last ul{border:none;width:161px;}<br />
<br />
</style><br />
<div class="outer"><br />
<br />
<ul id="nav"><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey">&nbsp;&nbsp;Home</a></p><br />
<br />
<br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Team">&nbsp;&nbsp;Team</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li><br />
</ul><br />
</li><br />
<li><br />
<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li><br />
</ul><br />
</li><br />
<br />
<li class="last"><br />
<p><br /><br />
<a href="https://2010.igem.org/Team:Tec-Monterrey /Sponsors">&nbsp;&nbsp;Sponsors</a></p><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Platinum ">Platinum </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gold ">Gold </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Silver">Silver </a></li><br />
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Thanks">Special Thanks</a></li> </ul><br />
</li><br />
</ul><br />
<br />
</div><br />
<br />
</div><br />
<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br><br><br><br><br />
<br />
<br />
<div class="righ"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png"><br />
</div><br />
<div class="bdy"><br />
<p align="center"><br />
<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> <br />
<br><br><br><br />
<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a><br />
<br><br><br><br />
<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a><br />
<br><br />
<br><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"></div><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png"><br />
</div><br />
<div class="bdy"><br />
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
</div><br />
<br />
<div class="texteando"><br />
<div class="hd"><div class="g"></div></div><br />
<div class="top"><br />
<div class="top"><br />
<img src="https://static.igem.org/mediawiki/igem.org/d/dc/Humanpractices.png" border="0" ><br />
<div class="bdy"><br />
<br><br />
<p class="blahimgder"><br />
<ol class="menud"><br />
<a href="#introduction"><li>Introduction</a></li><br />
<a href="#regulations"><li>Regulations in Mexico related to General Health Law</a></li><br />
<ol class="menue"><br />
<a href="#overview"><li>Overview</a></li><br />
<a href="#general"><li>General requirements for labeling importation samples<br />
</a></li><br />
<a href="#biotech"><li>For Biotechnology Labeling</a></li><br />
<br />
<a href="#regreq"><li>Regulations and Requirements</a></li><br />
<a href="#import"><li>Import Procedures</a></li><br />
<br />
</ol><br />
<a href="#manual"><li>Importation manual: a step-by-step guide<br />
</a></li><br />
<ol class="menue"><br />
<a href="#document"><li>Document Requirements</a></li><br />
<a href="#step1"><li>Step 1</a></li><br />
<a href="#step2"><li>Step 2</a></li><br />
<a href="#step3"><li>Step 3</a></li><br />
<a href="#step4"><li>Step 4</a></li><br />
<a href="#step5"><li>Step 5</a></li><br />
</ol><br />
<a href="#recommendations"><li>Recommendations</a></li><br />
</ol><br />
</p><br />
<br><br />
<h2><a name="introduction"></a>Introduction</h2><br />
<p class="blah"><br />
This year was the first time that the team Tec-Monterrey participated in iGEM. We are only the third university in Mexico to ever participate in this competition, and one of the few in Latin American. As our ideas slowly began to shape up into a real project, one of the greatest obstacles that we had to overcome, and perhaps one that we had naively not expected, was the Mexican Customs. <br />
</p><br />
<br />
<p class="blah"><br />
We were surprised to discover that our government doesn’t have a defined protocol for the importation of biological material. The Customs Officers freaked out on reading the words “gene”, “DNA” or “bacteria”, and even if the contents were labeled correctly as non-toxic, non-carcinogenic, non-teratogenic, etc… we had several packages delayed or permanently detained. We were constantly sent to different departments, and finally realized that the mandatory permits should have been requested with months in advance and that they naturally required the payment of a considerable fee.<br />
</p><br />
<p class="blah"><br />
On speaking to researchers in our own and other universities, we learned that we were not alone on this difficulty. Scientists with enough money pay specialized agencies to arrange the importation of their biological material, which may cost more than the material to be imported! Researchers with not such an ample budget simply rely on luck and hope that their package will go unnoticed if undeclared. Clearly, this complication not only slowed down our iGEM project; it delays the progress of science in our country altogether and reflects the lack of understanding of the biological language from our authorities. <br />
</p><br />
<p class="blah"><br />
Synthetic biology is an emerging science in our country. We know that it is only a short matter of time before more Mexican teams participate in future iGEM competitions, and we believe that we can ease their experience by sharing our own. We have created a quick user manual with the basic instructions needed to fill out the necessary paperwork to receive synthetic DNA donations – such as the iGEM Kit or BioBricks form the Registry.We hope that this information will be valuable not only for future Mexican iGEM teams, but also for any researcher working in the field of synthetic biology in Mexico. <br />
</p><br />
<p class="blah"><br />
We finally conclude with a list of recommendations that may improve the process of importation of biological material into our country, and that if implemented will greatly facilitate and speed up the progress of science in Mexico. We have submitted this document to our local congresswoman, Brenda Velázquez Valdez, and hope that our leaders will be made aware our views and take them into account for future laws. <br />
</p><br />
<h2><a name="regulations"></a> Regulations in Mexico related to General Health Law<br />
</h2><br />
<h4><a name="overview"></a>Overview</h4><br />
<p class="blah"><br />
The General Health Law assigns the Mexican Ministry of Health (Secretaría de Salud) with the responsibility of issuing regulations related to human health. In the case of importations, the importer must show all the required documentation under implemented regulations by the General Health Law, and must include a health certificate issued by authorities in the country of origin. The Ministry may also randomly sample and inspect imported products to ensure compliance with Mexican requirements. Mexican regulations related to human health are issued through the Ministry of Health’s regulatory agency, by the Federal Commission for the Protection against Sanitary Risks (COFEPRIS); its website <a href="http://www.cofepris.gob.mx/">www.cofepris.gob.mx</a>.<br />
</p><br />
<br><br />
<br />
<h4><a name="general"></a>General requirements for labeling importation samples<br />
</h4><br />
<p class="blah"><br />
There are two options for labeling:<br />
<ol class="menud"><br />
<li>The original English container can be used as long as a sticker containing all the labeling requirements in Spanish is attached. Stickers are permitted as long as they remain attached to the container.</li><br />
<li>The product can be packaged for the local Mexican market using Spanish. </li><br />
</ol><br />
</p><br />
<br><br />
<br />
<h4><a name="biotech"></a>Biotechnology labeling</h4><br />
<p class="blah"><br />
Currently there is not a specific NOM (Mexican Official Norm or standard) for labeling GMO’s or products containing GMO’s. However, the industry has agreed that yellow corn must be identified as such in the package. These products are subject to general labeling requirements.<br />
</p><br />
<br><br />
<br />
<h4><a name="regreq"></a>Regulations and Requirements Based on Public Health Risks (SSA or SALUD)<br />
</h4><br />
<p class="blah"><br />
<br />
The Federal Commission for the Sanitary Risk Protection (COFEPRIS) is the regulatory agency in charge of the control and surveillance of food and beverages, food supplements, tobacco, plant nutrients, toxic or dangerous substances, biotechnology products, and pesticides among others types of products. <br />
</p><br />
<br />
<p class="blah"><br />
<br />
The <i>Treaty that establishes the classification and codification of products, the importation of which is subject to regulations from the Mexican Ministry of Health SSA</i>, issued on September 27, 2007, and last updated on June 25, 2009, lists the tariff numbers of the products that are subject to SSA regulation. These products require either an import permit “PERMISO Sanitario de Importación” or simply an import notification “AVISO Sanitario de Importación” to COFEPRIS.<br />
</p><br />
<br />
<p class="blah"><br />
The most important thing for the U.S. exporter is to determine the exact tariff classification number. This number will define the regulatory agency and all the documentation necessary to get import approval. <b>In the case of DNA, this classification number (fracción arancelaria) is 29371999 “ácido desoxiribonucleico”.</b> The importer of products subject to SSA regulations must be registered with SALUD and is responsible for securing all the necessary documentation for a successful importation process. <br />
</p><br />
<br />
<br />
<p class="blah"><br />
The importer of products subject to SSA regulations must be registered with SALUD and is responsible for securing all the necessary documentation for a well done import process. <br />
<br />
</p><br />
<br />
<br />
<br><br />
<h4><a name="import"></a>Import Procedures</h4><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/d/d6/Importproceduresp.png"><br />
</center><br />
<h2><a name="manual"></a>Importation manual: a step-by-step guide</h2><br />
<p class="blah"><br />
If you are a Mexican team from a public or private university and wish toreceive BioBricks from the Registry or synthetic DNA as a donation, then you must begin by obtaining a health permit, three months in advance to avoid setbacks. It is important to know that the regulatory agency for this permission is the COFEPRIS, the Federal Commission for the Protection Against Sanitary Risks in Mexico. <br />
</p><br />
<p class="blah"><br />
The code for this permission is: COFEPRIS-01-010-E<br />
</p><br />
<br />
<p class="blah"><br />
*It is important that the items be declared as a donation. As a $0.00 price cannot be officially declared, we suggest claiming a cost of $1 or $10 USD.<br />
</p><br />
<br />
<h4><a name="document"></a>Document requirements</h4><br />
<p class="blahimgizq"><br />
<ul class="lista"><br />
<li> Donation letter given and signed by the donor with recent date. This document must be sent on letterhead paper. </li><br />
<li> Acceptance letter of the donation given and signed by the responsible of your team in your university. This document must be sent on letterhead paper with recent date and it should contain a statement of no commercialization. </li><br />
<li> Copy of professional license of the doctor responsible for the donation.</li><br />
<li>Letter that establishes and notifies clearly the use of the product that you want to import. </li><br />
<li> Application form named “Solicitudes”.</li><br />
</ul><br />
<br><p class="blah"><br />
To get started with this process you must fill out properly the application form “Solicitudes”, which is in the web page: <a href="http://www.cofepris.gob.mx" target="blank"> www.cofepris.gob.mx</a><br />
</p><br />
<br />
<p class="blah"><br />
Particularly you must fill the next numbers in the application form <b>“Solicitudes”</b>:<br />
</p><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/3/3d/Solicitudes.jpg"><br />
</center><br />
<br><br />
<br><br />
<h4><a name="step1"></a>Step 1</h4><br />
<p class="blah"><br />
<center><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/87/Step1.png"><br />
</center><br />
</p><br />
<br><br />
<br />
<h4><a name="step2"></a>Step 2</h4><br />
<p class="blah"><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/9/9f/Step2.png"><br />
</center><br />
</p><br />
<br><br />
<br />
<h4><a name="step3"></a>Step 3</h4><br />
<p class="blah"><br />
This section is regarding product information. The fields that must be filled out are: 1, 2, 3, 4, 5, 6, 7, 8, 10,11, 15, 17, 18, 35, 36, 37.<br />
<br><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/0/08/Step3.png"><br />
</center><br />
<br />
</p><br />
<br><br />
<br />
<h4><a name="step4"></a>Step 4</h4><br />
<p class="blah"><br />
This section is regarding import registry.<br />
<br><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/a/ad/Step4.png"><br />
</center><br />
<br />
</p><br />
<br><br />
<br />
<h4><a name="step5"></a>Step 5</h4><br />
<p class="blah"><br />
<br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/4/4f/Step5.png"><br />
</center><br />
<br />
</p><br />
<br />
<br><br />
<br />
<p class="blah"><br />
Download the Manual as a PDF <a href= "https://static.igem.org/mediawiki/igem.org/4/48/Importation_manual.pdf" target="blank">here.</a><br />
</p><br />
<br />
<br><br />
<h2><a name="recommendations"></a>Recommendations</h2><br />
<p class="blah"><br />
We finally conclude with a list of ideas that may improve the process of importation of biological material into our country, and that if implemented will greatly facilitate and speed up the progress of science in Mexico. We have voiced these recommendations to our local congresswoman, <a href="http://www.congreso-nl.gob.mx/potentiaweb/portal/genera/VistasV2_1/PlantillasV2/congreso.asp?Portal=2&View=2&Origen=4822&MenuActivo=7&MenuActivo2=0">Brenda Velázquez Valdez</a>, and hope that our leaders will be made aware our views and take them into account for future laws. <br />
<br />
</p><br />
<p class="blah"><br />
<br />
<ol class="menud"><br />
<br />
<li>Any researcher or university should be able to legally import non-hazardous biological material without necessarily having to hire an agency. An easy step-by-step guide for the correct labeling, and necessary paperwork and proceedings for the importation of biological material should be made available on line and the required forms should be more accessible to the general public. </li><br />
<br />
<li>Every Customs Agent should have a minimal understanding of biology and a basic knowledge of scientific terms, such as DNA, gene, synthetic, bacteria, strain, vial, so that these terms don’t immediately suggest a biological hazard or weapon. </li><br />
<br />
<li>A scientific committee that reviews the laws governing the importation of biological material and advises on relevant changes should be established. The actual laws need to be modernized in order to encourage scientific research and international collaboration. </li><br />
<br />
<li>A new tariff classification for biological products needs to be created. The current classification is very ambiguous. For example, we were once asked to classify our bacterial strain as belonging to either the plant or animal kingdom. This classification should take into account biohazard levels, and each class should be clearly defined and independent.</li><br />
<br />
<li>When importing or exporting biological material, a simple declaration form should be initially filled, stating the contents and biohazard level. If there is no potential hazard and the item is correctly labeled, there should be no reason for it to be detained. </li><br />
<br />
<li>Donated biological material ($0.00 value) pays no taxes, but we propose to reduce the taxes on biological material imported with investigation or experimental purposes only, especially if it the research is linked to undergraduate or graduate students seeking no monetary gain and usually working with low budgets. </li><br />
<br />
<li>Science moves at an extraordinarily fast pace, and it is outrageous that the approval of importation permits in our country can take up to twelve months. Nowadays, requesting these permits from a place other than Mexico City takes an even longer time, because the paperwork needs to be sent the capital city and then back. This time lapse can easily be accelerated if the logistics are improved and state offices are installed. </li><br />
</ol><br />
</p><br />
</div><br />
<div class="ft"><div class="c"></div></div><br />
<div class="bgr"><br><br />
</div><br />
</div><br />
<br />
<div class="ft"> </div><br />
</div><br />
</div> <br />
</html></div>
Andreshuerta