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- 23:32, 26 October 2010 (diff | hist) Team:Toronto/Notebook
- 23:30, 26 October 2010 (diff | hist) N Toronto/15 September 2010 (New page: Image:Picture 15.jpg Image:Picture 16.jpg)
- 23:30, 26 October 2010 (diff | hist) N File:Picture 16.jpg (top)
- 23:30, 26 October 2010 (diff | hist) N File:Picture 15.jpg (top)
- 23:27, 26 October 2010 (diff | hist) N Toronto/14 September 2010 (New page: Image:Picture 13.jpg Image:Picture 14.jpg)
- 23:27, 26 October 2010 (diff | hist) N File:Picture 14.jpg (top)
- 23:27, 26 October 2010 (diff | hist) N File:Picture 13.jpg (top)
- 23:23, 26 October 2010 (diff | hist) N Toronto/10 September 2010 (New page: Image:Picture 12.jpg)
- 23:23, 26 October 2010 (diff | hist) N File:Picture 12.jpg (top)
- 23:20, 26 October 2010 (diff | hist) N Toronto/9 September 2010 (New page: Transformations worked in: E4 BL21 → took 3 samples DH5a → took 1 sample Lawns in all DH5a cells→ re-streaked using 4-quadrant technique -Make more Amp plates tomorrow Tomorro...) (top)
- 23:20, 26 October 2010 (diff | hist) N Toronto/8 September 2010 (New page: Image:Picture 11.jpg)
- 23:19, 26 October 2010 (diff | hist) N File:Picture 11.jpg (top)
- 23:18, 26 October 2010 (diff | hist) N Toronto/2 September 2010 (New page: Image:Picture 9.jpg Image:Picture 10.jpg)
- 23:17, 26 October 2010 (diff | hist) N File:Picture 10.jpg (top)
- 23:17, 26 October 2010 (diff | hist) N File:Picture 9.jpg (top)
- 23:13, 26 October 2010 (diff | hist) N Toronto/30 August 2010 (New page: Image:Picture 7.jpg Image:Picture 8.jpg)
- 23:13, 26 October 2010 (diff | hist) N File:Picture 8.jpg (top)
- 23:13, 26 October 2010 (diff | hist) N File:Picture 7.jpg (top)
- 23:10, 26 October 2010 (diff | hist) N Toronto/25 August 2010 (New page: Image:Picture 6.jpg)
- 23:10, 26 October 2010 (diff | hist) N File:Picture 6.jpg (top)
- 23:08, 26 October 2010 (diff | hist) N Toronto/24 August 2010 (New page: Image:Picture 5.jpg)
- 23:08, 26 October 2010 (diff | hist) N File:Picture 5.jpg (top)
- 23:06, 26 October 2010 (diff | hist) N Toronto/23 August 2010 (New page: Transformed bacteria were plated on Thursday Aug 19, 2010 It seems like no cultures grew anywhere except on 'LB+C” plate under: “E4-RBS F2”, “E5a-RBS F2” and maybe “E2-RBS F2...) (top)
- 23:05, 26 October 2010 (diff | hist) N Toronto/19 August 2010 (New page: Image:Picture 3.jpg Image:Picture 4.jpg)
- 23:05, 26 October 2010 (diff | hist) N File:Picture 4.jpg (top)
- 23:05, 26 October 2010 (diff | hist) N File:Picture 3.jpg (top)
- 23:00, 26 October 2010 (diff | hist) N Toronto/18 August 2010 (New page: Fusion PCR Annealing temperature=54 C Fusion Construct 1 DNA Templates(Fusion primer amplified): E1 - 1uL Term1 - 5.05uL J23106 - 5.05uL E2 - 1uL E3 - 1uL Term2 - 5.05u...) (top)
- 22:54, 26 October 2010 (diff | hist) N Toronto/17 August 2010 (New page: → Transformed BL21 cells - 9 samples: E1-5b, J23106, positive and negative controls -Pipetted 100uL of BL21 cells into each centrifuge tube (x9) -Added 1uL of DNA to all tubes except ...) (top)
- 22:51, 26 October 2010 (diff | hist) N Toronto/16 August 2010 (New page: Image:Picture 1.jpg Image:Picture 2.jpg)
- 22:51, 26 October 2010 (diff | hist) N File:Picture 2.jpg
- 22:49, 26 October 2010 (diff | hist) N File:Picture 1.jpg (top)
- 22:47, 26 October 2010 (diff | hist) N Toronto/13 August 2010 (New page: -Plates Blank?!?! -PET vector miniprepped (50uL, ??? mg/uL) -PaD100 vector miniprepped (50uL, 172 ug/uL) Digestion of PET vector for E1-->E5b insertion (Prepared through PCR) Taking:...) (top)
- 22:46, 26 October 2010 (diff | hist) N Toronto/12 August 2010 (New page: Re-transforming biobrick vector with E1-->E5b to BL21 cells instead of RosDE3 -Plated 3 & 12 uL on LB+C plate for each enzyme -Incubating the culture overnight at 37C) (top)
- 22:46, 26 October 2010 (diff | hist) N Toronto/11 August 2010 (New page: Running PCR for PET-expression vector. Primers arrived yesterday -->using 1uL of stock DNA for E1-->E5b 10x reaction buffer 2.5uL ddH2O 17.15uL Fwd primer 0.2uL Rvs primer 0.2uL dNT...) (top)
- 22:45, 26 October 2010 (diff | hist) N Toronto/10 August 2010 (New page: Plates: LB+C--> worked C--> didn't work at all +ve-->Amp plate did not work Re-running transformation with new agar plates tomorrow Made Agar plates (7) with chloramphenicol resistanc...) (top)
- 22:44, 26 October 2010 (diff | hist) N Toronto/9 August 2010 (New page: -Transformation of Competent Cells (RosDE3) → Cells thawed from -80 C -used H2O for -ve transformation control -used PUC19 plasmid for transformation +ve control (Amp resistance) ...) (top)
- 22:43, 26 October 2010 (diff | hist) N Toronto/6 August 2010 (New page: -Preparation of pSBIE3 for submission Digestion Enzyme DNA is in 0.1ug/uL solution → 1uL of each enzyme DNA taken (100ng) for digestion 2.5 uL 10x NEB buffer 4 (best with both EcoRI...) (top)
- 22:41, 26 October 2010 (diff | hist) N File:Tables 1 and 2.jpg (top)
- 22:34, 26 October 2010 (diff | hist) N Toronto/4 August 2010 (New page: -Received fusion primers in mail Resuspended the enzyme DNA: -Spinned the 6 tubes briefly ( a few seconds ~10s) to ensure all the contents were on the bottom of the tube -Added 50uL of...) (top)
- 22:34, 26 October 2010 (diff | hist) N Toronto/3 August 2010 (New page: Performing a Kill Assay -Making 4 different catechol concentrations: 0mM, 0.5mM, 1mM, and 2.5mM -Set the shaker to 30C -Making a catechol stock solution-->0.05M Analytical balance ...)
- 22:27, 26 October 2010 (diff | hist) N Toronto/29 July 2010 (New page: Catechol based cultures 0mM, 0.5mM, 1mM, 2.5mM (all with chlorophecol [5uL]))
- 22:27, 26 October 2010 (diff | hist) N Toronto/28 July 2010 (New page: -put cell for overnight @ 2pm OD600 = 1.081, assumed to be close to OD600=1) (top)
- 22:26, 26 October 2010 (diff | hist) N Toronto/21 July 2010 (New page: Overnight cell culture- OD600= 1.099 3pm- put cells in 0mM, 0.5mM, 1mM, and 2.5 mM catechol solution -also plated 0hr and left in 37C incubator -left catechol-exposed culture in 30C in...) (top)
- 22:25, 26 October 2010 (diff | hist) Toronto/19 July 2010
- 22:20, 26 October 2010 (diff | hist) Toronto/19 July 2010
- 22:20, 26 October 2010 (diff | hist) N Toronto/19 July 2010 (New page: Grew 5mL ROSDE3 (5uL) in LB (5mL) + ChL (5uL) in 37 C shaker overnight for kill assay 0.0275 = 0.0230 5mL x x=4.18mL)
- 22:19, 26 October 2010 (diff | hist) N Toronto/16 July 2010 (New page: Cell counts → too hard to count) (top)
- 22:18, 26 October 2010 (diff | hist) N Toronto/15 July 2010 (New page: Grew RosDE3 in 4 tubes (70 uL E. coli + 5mL LB + 5uL ChL) in 37 C Shaker at 10:55am Wait for the Log phase of the bacteria O.D 0.483 blank O.D 0.502 0.5mM O.D 0.509 1mM O.D 0.491 2....) (top)
- 22:01, 16 July 2010 (diff | hist) Team:Toronto/Project
- 21:57, 16 July 2010 (diff | hist) Team:Toronto/Project
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