User contributions
From 2010.igem.org
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- 07:06, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:05, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:05, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 06:55, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 06:56, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/26 August 2010 (→Module I, DT and Fan's part:)
- 06:56, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/25 August 2010 (→Module I, DT and Fan's part:)
- 06:56, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/19 August 2010 (→Module I, DT and Fan's part:)
- 06:56, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/18 August 2010 (→Module I, DT and Fan's part:)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/17 August 2010 (→Module I, DT and Fan's part:)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/16 August 2010 (→Module I, DT and Fan's part:)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/15 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/14 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/13 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:54, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/12 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:54, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/11 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:54, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/10 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:54, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/9 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:53, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/8 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:53, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/7 August 2010 (→Module I, DT and Fan's part:)
- 06:53, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/6 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:53, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/5 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:52, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/4 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:52, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/3 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:52, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/2 August 2010 (→Module I, DT and Fan's part:)
- 06:51, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/28 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:51, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/27 July 2010 (→Module I, DT and Fan's part:)
- 06:51, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/19 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:50, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/18 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:50, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/15 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:50, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/14 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:50, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/13 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:49, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/11 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:49, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/10 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:48, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/9 July 2010 (→Module I, DT and Fan's part:) (top)
- 10:33, 3 September 2010 (diff | hist) N File:10-8-26.jpg (top)
- 10:32, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/26 August 2010 (New page: == Module I, DT and Fan's part: == Run a colony PCR with universal primers. Amplify the bacteria at the same time. PCR system (SuperMix): H2O 8μl primer1 1μl primer2 1μ...)
- 10:30, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/25 August 2010 (→Module I, DT and Fan's part:)
- 10:29, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/25 August 2010 (New page: == Module I, DT and Fan's part: == Ligate PEMK with C. Using the PEMK①. Double digestion system: chlr: H2O 5μl buffer Tango 2μl chlr 10μl KpnI ...)
- 10:27, 3 September 2010 (diff | hist) N File:10-8-19.jpg (top)
- 10:27, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/19 August 2010 (New page: == Module I, DT and Fan's part: == Use the universal primers to detect. == result == Image:10-8-19.jpg Only the PEM+K 1 and 5 are positive.)
- 10:26, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/18 August 2010 (New page: == Module I, DT and Fan's part: == Pick 6 colonies each from PEM+K and PC+K plate. Amplify and purify the plasmids. Measure the concentration.)
- 10:25, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/17 August 2010 (New page: == Module I, DT and Fan's part: == Ligate again from PEM and PC by adding K fragments. Double digestion system: kan: H2O 9μl buffer BamHI 5μl kan 30μl KpnI ...)
- 10:23, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/16 August 2010 (New page: == Module I, DT and Fan's part: == Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown b...)
- 10:22, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/15 August 2010 (New page: == Module I, DT and Fan's part: == PCR and double digestion to detect. Double digestion system: H2O 8μl buffer tango 4μl plasmid 5μl EcoRI 1μl BamHI ...)
- 10:21, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/14 August 2010 (New page: == Module I, DT and Fan's part: == Pick out 6 colonies to amplify. After 10 hours, purify the plasmids and measure the concentration.)
- 10:19, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/13 August 2010 (New page: == Module I, DT and Fan's part: == Use the PEM② and PKC① to go on the further experiments. Double digestion. Double digestion system: PEM, PKC: H2O 25.5μl buffer tang...)
- 10:18, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/12 August 2010 (→result)
- 10:17, 3 September 2010 (diff | hist) N File:10-8-12.jpg (top)
- 10:16, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/12 August 2010 (→== result ==)
- 10:16, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/12 August 2010 (New page: == Module I, DT and Fan's part: == Use the K-up and K-do to detect the P+K+C. Meanwhile use the digestion protocol to ensure the result. Double digestion system: P+E+M: H2O ...)
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