User contributions
From 2010.igem.org
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 01:16, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/LB Media (New page: To 700mL of deionized water add: Tryptone 10g Yeast Extract 5g NaCl 10g Fill to 1L '''AUTOCLAVE''' Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</...)
- 01:16, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Preparation of Competent Cells (New page: ==Materials== *Plate of cells to be made competent *TSS buffer *LB media *Ice *Glassware & Equipment *Falcon tubes *500μl Eppendorf tubes, on ice *200ml conical flask *200μl pipetman o...) (top)
- 01:12, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/O/N Culture (New page: '''REMEMBER TO BE STERILE''' *Take 5mL LB and put in a round bottom tube *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube *'''MAKE SURE YOU ADD THE R...) (top)
- 01:11, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Transformation (New page: #Thaw cells on ice and pipette 25uL aliquots into tubes on ice #Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). #*Note: If you are adding small volumes (~1&...) (top)
- 01:11, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/New Part Design(PCR) (New page: The following protocol is for creating a new biobricks part from a protein coding sequence. '''Primer Design''' # Design a forward primer to your new BioBrick part comprised of the BioBri...) (top)
- 01:10, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Ethanol Precipitation (New page: ==Material== * 100% ethanol * 3M sodium acetate at pH 5.2 * -20°C or -80°C freezer * tabletop centrifuge ==Procedure: PREFERRED -20°C Protocol== #Add the following to your sample: #*2....) (top)
- 01:10, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Kit to Stock Plasmid (New page: '''EXTRACTION FROM KIT''' #With a pipette tip, punch a hole through the foil cover into the corresponding well to the Biobrick™-standard part that you want. Make sure you have properly ...)
- 01:09, 23 October 2010 (diff | hist) Team:Northwestern/Protocol/Quikchange (from primers to colonies! (→Primers)
- 01:08, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Quikchange (from primers to colonies! (New page: ==Primers== I use [http://mekentosj.com/enzymex/ enzyme x] to work with DNA sequences, it is installed on all the macs and has nice translation and reverse complement functions. Many of ...)
- 01:07, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 01:07, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 01:01, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 00:54, 23 October 2010 (diff | hist) Team:Northwestern/SideProject
- 13:26, 21 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/21/10)
- 13:25, 21 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/20/10)
- 13:23, 21 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/19/10)
- 21:06, 19 October 2010 (diff | hist) Team:Northwestern/Links
- 01:01, 19 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/17/10)
- 01:00, 19 October 2010 (diff | hist) Team:Northwestern/Notebook
- 00:52, 19 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/16/10)
- 00:49, 19 October 2010 (diff | hist) Reagents (top)
- 00:48, 19 October 2010 (diff | hist) Team:Northwestern/Links
- 00:48, 19 October 2010 (diff | hist) Team:Northwestern/Links (→MediaWiki Resources)
- 00:46, 19 October 2010 (diff | hist) Team:Northwestern/Links
- 00:45, 19 October 2010 (diff | hist) Team:Northwestern/Links (→Research Resources)
- 00:44, 19 October 2010 (diff | hist) Team:Northwestern/Links (→Protocol-Related)
- 03:08, 17 October 2010 (diff | hist) Team:Northwestern/Team
- 03:06, 17 October 2010 (diff | hist) Team:Northwestern/Team
- 03:03, 17 October 2010 (diff | hist) Team:Northwestern/Team
- 20:32, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Basic iGEM Resources)
- 20:31, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Basic iGEM Resources)
- 20:30, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Protocol-Related)
- 20:27, 16 October 2010 (diff | hist) Team:Northwestern/Links
- 20:20, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Basic iGEM Resources)
- 20:16, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Basic iGEM Resources)
- 19:53, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 19:42, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements
- 19:42, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 19:24, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Sponsors)
- 19:19, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Sponsors)
- 18:33, 16 October 2010 (diff | hist) Team:Northwestern/Protocol (→Prepping DNA from the kit plates)
- 18:33, 16 October 2010 (diff | hist) Team:Northwestern/Protocol (→Prepping DNA from the kit plates)
- 18:31, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 18:28, 16 October 2010 (diff | hist) Team:Northwestern/SideProject (→Ethics)
- 18:27, 16 October 2010 (diff | hist) Team:Northwestern/SideProject
- 18:24, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Collaboration)
- 18:23, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Lethbridge)
- 18:18, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 18:17, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 18:08, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Sponsors)
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)