User contributions
From 2010.igem.org
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- 01:16, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/LB Media (New page: To 700mL of deionized water add: Tryptone 10g Yeast Extract 5g NaCl 10g Fill to 1L '''AUTOCLAVE''' Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</...)
- 01:16, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Preparation of Competent Cells (New page: ==Materials== *Plate of cells to be made competent *TSS buffer *LB media *Ice *Glassware & Equipment *Falcon tubes *500μl Eppendorf tubes, on ice *200ml conical flask *200μl pipetman o...) (top)
- 01:12, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/O/N Culture (New page: '''REMEMBER TO BE STERILE''' *Take 5mL LB and put in a round bottom tube *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube *'''MAKE SURE YOU ADD THE R...) (top)
- 01:11, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Transformation (New page: #Thaw cells on ice and pipette 25uL aliquots into tubes on ice #Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). #*Note: If you are adding small volumes (~1&...) (top)
- 01:11, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/New Part Design(PCR) (New page: The following protocol is for creating a new biobricks part from a protein coding sequence. '''Primer Design''' # Design a forward primer to your new BioBrick part comprised of the BioBri...) (top)
- 01:10, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Ethanol Precipitation (New page: ==Material== * 100% ethanol * 3M sodium acetate at pH 5.2 * -20°C or -80°C freezer * tabletop centrifuge ==Procedure: PREFERRED -20°C Protocol== #Add the following to your sample: #*2....) (top)
- 01:10, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Kit to Stock Plasmid (New page: '''EXTRACTION FROM KIT''' #With a pipette tip, punch a hole through the foil cover into the corresponding well to the Biobrick™-standard part that you want. Make sure you have properly ...)
- 01:09, 23 October 2010 (diff | hist) Team:Northwestern/Protocol/Quikchange (from primers to colonies! (→Primers)
- 01:08, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Quikchange (from primers to colonies! (New page: ==Primers== I use [http://mekentosj.com/enzymex/ enzyme x] to work with DNA sequences, it is installed on all the macs and has nice translation and reverse complement functions. Many of ...)
- 01:07, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 01:07, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 01:01, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 00:54, 23 October 2010 (diff | hist) Team:Northwestern/SideProject
- 13:26, 21 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/21/10)
- 13:25, 21 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/20/10)
- 13:23, 21 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/19/10)
- 21:06, 19 October 2010 (diff | hist) Team:Northwestern/Links
- 01:01, 19 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/17/10)
- 01:00, 19 October 2010 (diff | hist) Team:Northwestern/Notebook
- 00:52, 19 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/16/10)
- 00:49, 19 October 2010 (diff | hist) Reagents (top)
- 00:48, 19 October 2010 (diff | hist) Team:Northwestern/Links
- 00:48, 19 October 2010 (diff | hist) Team:Northwestern/Links (→MediaWiki Resources)
- 00:46, 19 October 2010 (diff | hist) Team:Northwestern/Links
- 00:45, 19 October 2010 (diff | hist) Team:Northwestern/Links (→Research Resources)
- 00:44, 19 October 2010 (diff | hist) Team:Northwestern/Links (→Protocol-Related)
- 03:08, 17 October 2010 (diff | hist) Team:Northwestern/Team
- 03:06, 17 October 2010 (diff | hist) Team:Northwestern/Team
- 03:03, 17 October 2010 (diff | hist) Team:Northwestern/Team
- 20:32, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Basic iGEM Resources)
- 20:31, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Basic iGEM Resources)
- 20:30, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Protocol-Related)
- 20:27, 16 October 2010 (diff | hist) Team:Northwestern/Links
- 20:20, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Basic iGEM Resources)
- 20:16, 16 October 2010 (diff | hist) Team:Northwestern/Links (→Basic iGEM Resources)
- 19:53, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 19:42, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements
- 19:42, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 19:24, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Sponsors)
- 19:19, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Sponsors)
- 18:33, 16 October 2010 (diff | hist) Team:Northwestern/Protocol (→Prepping DNA from the kit plates)
- 18:33, 16 October 2010 (diff | hist) Team:Northwestern/Protocol (→Prepping DNA from the kit plates)
- 18:31, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 18:28, 16 October 2010 (diff | hist) Team:Northwestern/SideProject (→Ethics)
- 18:27, 16 October 2010 (diff | hist) Team:Northwestern/SideProject
- 18:24, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Collaboration)
- 18:23, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Lethbridge)
- 18:18, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 18:17, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 18:08, 16 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→Sponsors)
- 18:01, 16 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/14/10)
- 17:55, 16 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/15/10)
- 17:51, 16 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/16/10)
- 17:48, 16 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/26/10)
- 17:47, 16 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/20/10)
- 03:15, 11 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/8/10)
- 03:14, 11 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/7/10)
- 03:14, 11 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/9/10)
- 03:13, 11 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/7/10)
- 03:11, 11 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/10/10)
- 03:11, 11 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/9/10)
- 03:11, 11 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/10/10)
- 00:18, 6 October 2010 (diff | hist) Team:Northwestern/Notebook
- 03:07, 3 October 2010 (diff | hist) Team:Northwestern (→Northwestern University)
- 03:02, 3 October 2010 (diff | hist) Team:Northwestern (→Sponsors)
- 02:57, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/3/10)
- 02:54, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/2/10)
- 02:52, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/23/10)
- 02:50, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/29/10)
- 02:45, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/25/10)
- 02:43, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/1/10)
- 02:42, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/28/10)
- 02:41, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/28/10)
- 02:40, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/26/10)
- 02:40, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/27/10)
- 02:36, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/30/10)
- 02:35, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/26/10)
- 02:34, 3 October 2010 (diff | hist) Team:Northwestern/Notebook
- 02:33, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/25/10)
- 02:32, 3 October 2010 (diff | hist) Team:Northwestern/Notebook
- 02:31, 3 October 2010 (diff | hist) Team:Northwestern/Notebook
- 02:30, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/21/10)
- 02:30, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/22/10)
- 02:29, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/20/10)
- 02:27, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/17/10)
- 02:25, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/17/10)
- 02:24, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/16/10)
- 02:23, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/15/10)
- 02:15, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/6/10)
- 02:13, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/1/10)
- 01:48, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→8/24/10)
- 01:44, 3 October 2010 (diff | hist) Team:Northwestern/Notebook (→9/20/10)
- 01:38, 3 October 2010 (diff | hist) Week15 9/19/10-9/25/10
- 01:38, 3 October 2010 (diff | hist) N Week15 9/19/10-9/25/10 (New page: Classes at Northwestern finally began! This complicated our functionality as a team due to scheduling. Thus, we paired up and began working on different portions of the project on our own....)
- 01:35, 3 October 2010 (diff | hist) N Brainstorming April-June 2010 (New page: At our initial meeting in April, we brainstormed a list of over 100 possible ideas. The ideas were sorted into their respective iGEM categories: Health/Medicine, Food/Energy, Environment, ...)
- 01:23, 3 October 2010 (diff | hist) Team:Northwestern/Notebook
- 17:05, 17 August 2010 (diff | hist) Team:Northwestern/Team
- 16:29, 27 July 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 16:28, 27 July 2010 (diff | hist) Team:Northwestern/Acknowledgements
- 14:50, 16 July 2010 (diff | hist) N File:Picture1.jpg (top)
- 14:50, 16 July 2010 (diff | hist) Team:Northwestern
- 16:15, 12 July 2010 (diff | hist) Team:Northwestern
- 17:33, 9 July 2010 (diff | hist) Ethanol Precipitation
- 17:32, 9 July 2010 (diff | hist) Ethanol Precipitation
- 17:29, 9 July 2010 (diff | hist) Ethanol Precipitation
- 17:23, 9 July 2010 (diff | hist) Ethanol Precipitation
- 17:22, 9 July 2010 (diff | hist) Ethanol Precipitation (→Procedure)
- 00:32, 3 July 2010 (diff | hist) N File:Timi.jpg (top)
- 00:32, 3 July 2010 (diff | hist) N File:Sean.jpg (top)
- 00:31, 3 July 2010 (diff | hist) N File:Ragan.jpg (top)
- 00:31, 3 July 2010 (diff | hist) N File:Matt.jpg (top)
- 00:31, 3 July 2010 (diff | hist) N File:Kevin.jpg (top)
- 00:30, 3 July 2010 (diff | hist) File:Ben.jpg (uploaded a new version of "Image:Ben.jpg") (top)
- 00:24, 3 July 2010 (diff | hist) N File:Ben.jpg
- 00:23, 3 July 2010 (diff | hist) Team:Northwestern/Team
- 21:46, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→7/1/10)
- 21:42, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→7/1/10)
- 21:40, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→6/28/10)
- 21:39, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→7/1/10)
- 21:38, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→7/1/10)
- 21:37, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→6/28/10)
- 21:36, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→6/30/10)
- 21:36, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→7/1/10)
- 21:36, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→7/1/10)
- 21:34, 1 July 2010 (diff | hist) Team:Northwestern/Notebook (→7/1/10)
- 15:46, 1 July 2010 (diff | hist) Team:Northwestern/Sponsor
- 20:43, 29 June 2010 (diff | hist) Team:Northwestern/References
- 20:42, 29 June 2010 (diff | hist) Team:Northwestern/References
- 16:11, 29 June 2010 (diff | hist) Ligations
- 16:10, 29 June 2010 (diff | hist) Ligations
- 16:07, 29 June 2010 (diff | hist) Ligations
- 16:06, 29 June 2010 (diff | hist) N File:Insert.jpg (top)
- 16:05, 29 June 2010 (diff | hist) Ligations
- 16:02, 29 June 2010 (diff | hist) N Ligations (New page: '''Reagents''' *T4 DNA ligase *10x T4 DNA Ligase Buffer *Deionized, sterile H2O *Purified, linearized vector (likely in H2O or EB) *Purified, linearized insert (likely in H2O or EB) '''E...)
- 15:50, 29 June 2010 (diff | hist) Rhodamine-Phalloidin/Calcofluor Staining (top)
- 15:50, 29 June 2010 (diff | hist) LIVE/DEAD® BacLight - Bacterial Viability Kit (top)
- 15:48, 29 June 2010 (diff | hist) LIVE/DEAD® BacLight - Bacterial Viability Kit
- 15:48, 29 June 2010 (diff | hist) LIVE/DEAD® BacLight - Bacterial Viability Kit
- 15:37, 29 June 2010 (diff | hist) N LIVE/DEAD® BacLight - Bacterial Viability Kit (New page: '''Kit Components''' *Component A, DMAO: 2x100 µL, 5 mM in DMSO *Component B, EthD-III: 2x150 µL, 2 mM in DMSO '''Preparation of Live and Dead Bacterial Suspensions as Controls''' #Gro...)
- 15:25, 29 June 2010 (diff | hist) Rhodamine-Phalloidin/Calcofluor Staining
- 15:24, 29 June 2010 (diff | hist) N Rhodamine-Phalloidin/Calcofluor Staining (New page: '''Reagents''' <br/> *Formaldehyde - Polysciences, 10% EM Grade Cat #04018 * 10XPBS - 80 grams NaCl + 2 grams KCl + 14.4 grams Na2HPO4 + 2.4 grams KH2PO4. pH upon dilution to 1X should be...)
- 15:21, 29 June 2010 (diff | hist) Team:Northwestern/Protocol
- 14:04, 29 June 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty)
- 03:52, 26 June 2010 (diff | hist) Team:Northwestern/Sponsor
- 20:04, 22 May 2010 (diff | hist) Team:Northwestern
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