User contributions
From 2010.igem.org
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- 23:57, 19 October 2010 (diff | hist) UTDallas/30 September 2010 (→September 30, 2010) (top)
- 23:56, 19 October 2010 (diff | hist) UTDallas/29 September 2010 (→September 29, 2010) (top)
- 23:56, 19 October 2010 (diff | hist) UTDallas/28 September 2010 (→September 28, 2010) (top)
- 23:56, 19 October 2010 (diff | hist) UTDallas/23 September 2010 (→September 23, 2010) (top)
- 23:55, 19 October 2010 (diff | hist) UTDallas/22 September 2010 (→September 22, 2010) (top)
- 23:54, 19 October 2010 (diff | hist) UTDallas/21 September 2010 (→September 21, 2010) (top)
- 23:53, 19 October 2010 (diff | hist) UTDallas/20 September 2010 (→September 20, 2010) (top)
- 23:53, 19 October 2010 (diff | hist) UTDallas/20 September 2010 (→September 20, 2010)
- 23:53, 19 October 2010 (diff | hist) UTDallas/20 September 2010 (→September 20, 2010)
- 23:52, 19 October 2010 (diff | hist) N UTDallas/17 October 2010 (New page: *The transformations worked, therefore the ligations worked. Gave pink colonies due to the LacI+RFP part. *Incubated one colony from each plate for making glycerol stock and miniprepping t...)
- 23:52, 19 October 2010 (diff | hist) N UTDallas/16 October 2010 (New page: *Made chloramphenicol plates *Transformed the ligations from yesterday. *I also realized that the ligations will give us the wrong product. We took a random part (BBa_J04450) with the pSB1...)
- 23:51, 19 October 2010 (diff | hist) N File:10-15.JPG (top)
- 23:51, 19 October 2010 (diff | hist) N File:10-14.JPG (top)
- 23:50, 19 October 2010 (diff | hist) N UTDallas/15 October 2010 (New page: *Miniprepped PyeaR+GFP and PyeaR+RFP. *Digested PyeaR+GFP, PyeaR+RFP, and pSB1C3, then ran a gel electrophoresis and gel purified. All of them were correct and gave good yields. *Ligated P...)
- 23:49, 19 October 2010 (diff | hist) N UTDallas/14 October 2010 (New page: *Took time-lapse fluorescence microscopy images of PyeaR+GFP with 10mM potassium nitrate. *Digested PyeaR+GFP and PyeaR+RFP, then ran a gel electrophoresis and purification. Neither part w...)
- 23:48, 19 October 2010 (diff | hist) N UTDallas/13 October 2010 (New page: *The transformations from yesterday show that Pu was not digested, therefore we will not end up making our own Pu+GFP part due to time constraints. *We incubated PyeaR+GFP to do a time-lap...)
- 23:47, 19 October 2010 (diff | hist) N UTDallas/12 October 2010 (New page: Today we transformed the parts ligated yesterday. We will see if they worked tomorrow.)
- 23:47, 19 October 2010 (diff | hist) N UTDallas/11 October 2010 (New page: We ligated Pu to GFP and RFP. We will transform tomorrow.)
- 14:26, 9 October 2010 (diff | hist) N File:10-8.JPG (top)
- 14:26, 9 October 2010 (diff | hist) N File:10-7.JPG (top)
- 14:25, 9 October 2010 (diff | hist) N File:9-30.JPG (top)
- 14:25, 9 October 2010 (diff | hist) UTDallas/8 October 2010 (top)
- 14:24, 9 October 2010 (diff | hist) UTDallas/7 October 2010
- 14:24, 9 October 2010 (diff | hist) UTDallas/6 October 2010 (top)
- 14:24, 9 October 2010 (diff | hist) UTDallas/30 September 2010
- 14:23, 9 October 2010 (diff | hist) UTDallas/29 September 2010
- 14:23, 9 October 2010 (diff | hist) UTDallas/28 September 2010
- 14:23, 9 October 2010 (diff | hist) N UTDallas/8 October 2010 (New page: *Miniprepped, digested, ran gel electrophoresis, and gel purified Pu. *Got good results, plan on ligating on Monday. center)
- 14:22, 9 October 2010 (diff | hist) N UTDallas/7 October 2010 (New page: *Performed the large scale titration of PyeaR+GFP with 0M, 10uM, 50uM, 100uM, 500uM, 1mM, and 10mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite. *Results show th...)
- 14:21, 9 October 2010 (diff | hist) N UTDallas/6 October 2010 (New page: *Incubated Pu, and PyeaR+GFP overnight.)
- 14:21, 9 October 2010 (diff | hist) N UTDallas/30 September 2010 (New page: *Incubate the PyeaR+reporters in 0, 10uM, 100uM, and 1mM potassium nitrate solutions, then observed under the fluorescence microscope. *The intensity of the fluorescence increased with in...)
- 14:20, 9 October 2010 (diff | hist) N UTDallas/29 September 2010 (New page: *Made glycerol stock and miniprepped PyeaR+GFP and PyeaR+RFP. *Incubated the PyeaR+reporters in 250uM Potassium Nitrate solution, and then observed under the fluorescence microscope. *All ...)
- 14:19, 9 October 2010 (diff | hist) N UTDallas/28 September 2010 (New page: *Tested Pu+GFP+Pr+XylR with xylene. Again had leakage, all had approximately the same intensity. *Probably a problem with the Pu+GFP part, therefore we will try to make our own Pu+GFP par...)
- 11:54, 28 September 2010 (diff | hist) N File:9-24.JPG (top)
- 11:53, 28 September 2010 (diff | hist) N File:9-23(2).JPG (top)
- 11:53, 28 September 2010 (diff | hist) N File:9-23.JPG (top)
- 11:52, 28 September 2010 (diff | hist) UTDallas/27 September 2010 (top)
- 11:52, 28 September 2010 (diff | hist) UTDallas/26 September 2010 (top)
- 11:52, 28 September 2010 (diff | hist) UTDallas/25 September 2010 (→September 22, 2010) (top)
- 11:51, 28 September 2010 (diff | hist) UTDallas/25 September 2010
- 11:51, 28 September 2010 (diff | hist) UTDallas/23 September 2010
- 11:50, 28 September 2010 (diff | hist) UTDallas/24 September 2010 (top)
- 11:49, 28 September 2010 (diff | hist) N UTDallas/26 September 2010 (New page: *The transformations worked. We had colonies only on the expected plates. *Incubating colonies overnight to get DNA tomorrow.)
- 11:49, 28 September 2010 (diff | hist) N UTDallas/25 September 2010 (New page: *Transformed the ligations into commercial DH5α.)
- 11:49, 28 September 2010 (diff | hist) N UTDallas/27 September 2010 (New page: *Miniprepped the colonies from yesterday. *Incubated Pu+GFP+Pr+XylR for aromatic testing tomorrow.)
- 11:48, 28 September 2010 (diff | hist) N UTDallas/24 September 2010 (New page: *We digested PyeaR with the new SpeI and PstI-HF that we received in the mail. *We then ran a gel (see picture) which gave the correct band, we only took the bottom portion for gel purific...)
- 11:48, 28 September 2010 (diff | hist) N UTDallas/23 September 2010 (New page: *Prepared glycerol stock of the transformed ligated parts, in case they are correct. *Miniprepped the transformed ligated parts, and then digested it. *We also digested another part using ...)
- 22:24, 26 September 2010 (diff | hist) UTDallas/22 September 2010
- 22:24, 26 September 2010 (diff | hist) UTDallas/21 September 2010
- 22:23, 26 September 2010 (diff | hist) UTDallas/20 September 2010
- 22:23, 26 September 2010 (diff | hist) UTDallas/17 September 2010
- 22:23, 26 September 2010 (diff | hist) UTDallas/16 September 2010 (top)
- 22:22, 26 September 2010 (diff | hist) UTDallas/17 September 2010
- 22:21, 26 September 2010 (diff | hist) N File:9-17.JPG (top)
- 22:20, 26 September 2010 (diff | hist) UTDallas/17 September 2010
- 22:20, 26 September 2010 (diff | hist) N UTDallas/22 September 2010 (New page: *The transformations had colonies! *We incubated in LB broth overnight to see if they are correct tomorrow)
- 22:19, 26 September 2010 (diff | hist) N UTDallas/21 September 2010 (New page: *Made carb. plates *Transformed the ligations)
- 22:19, 26 September 2010 (diff | hist) N UTDallas/20 September 2010 (New page: *Ligated PyeaR to GFP, RFP, and orange)
- 22:18, 26 September 2010 (diff | hist) N UTDallas/17 September 2010 (New page: *Diluted down (1:50 ratio) the Pu+GFP+Pr+XylR to incubate until they had an OD of 0.06-0.1 on our nanodrop. *Incubated the Pu+GFP+Pr+XylR with various aromatics, then observed under the fl...)
- 22:18, 26 September 2010 (diff | hist) N UTDallas/16 September 2010 (New page: *Made carb. plates *Miniprepped orange, GFP, and RFP *Transformed the ligations into commercial DH5α *Incubated Pu+GFP+Pr+XylR overnight)
- 12:08, 21 September 2010 (diff | hist) UTDallas/15 September 2010 (top)
- 12:08, 21 September 2010 (diff | hist) UTDallas/14 September 2010 (top)
- 12:08, 21 September 2010 (diff | hist) UTDallas/13 September 2010 (top)
- 12:07, 21 September 2010 (diff | hist) UTDallas/10 September 2010 (top)
- 12:07, 21 September 2010 (diff | hist) UTDallas/9 September 2010
- 12:06, 21 September 2010 (diff | hist) Template:UTDallasTop
- 12:05, 21 September 2010 (diff | hist) Team:UTDallas (→Track Selection)
- 12:05, 21 September 2010 (diff | hist) Team:UTDallas (→Project Description)
- 12:02, 21 September 2010 (diff | hist) UTDallas/15 September 2010
- 12:01, 21 September 2010 (diff | hist) UTDallas/15 September 2010
- 12:01, 21 September 2010 (diff | hist) N File:9-15(2).jpg (top)
- 12:00, 21 September 2010 (diff | hist) N File:9-15.jpg (top)
- 11:59, 21 September 2010 (diff | hist) N UTDallas/15 September 2010 (New page: *the transformations did not work from yesterday, so we decided to redigest the parts to make certain that they were actually cut with both enzymes. *We then ran some gels, both gels had ...)
- 11:59, 21 September 2010 (diff | hist) N UTDallas/14 September 2010 (New page: *we took the broth incubated overnight split it into four parts, three with either toluene, benzene, and xylene, and the other with nothing. *We let them incubate for a few hours and then...)
- 11:58, 21 September 2010 (diff | hist) N UTDallas/13 September 2010 (New page: *we tried ligating the PyeaR to the various reporters again. *We also incubated some Pu+GFP+Pr+XylR in broth overnight to test the promoter activity tomorrow)
- 11:58, 21 September 2010 (diff | hist) N UTDallas/10 September 2010 (New page: *Today we observed the transformations. *None of the ligations were transformed. *We had 14 colonies from the Pu GFP and Pr+XylR.)
- 11:57, 21 September 2010 (diff | hist) UTDallas/9 September 2010
- 11:57, 21 September 2010 (diff | hist) N UTDallas/9 September 2010 (New page: *We are going to transform the ligated parts (from yesterday) tomorrow)
- 11:56, 21 September 2010 (diff | hist) N UTDallas/8 September 2010 (New page: {{Template:UTDallasTop}} {{Template:UTDallasMenu}} <span style="color:#EDE8E8"> <html> <style type="text/css"> table, tr, td {background:transparent; border:0px;} table table table {backgr...) (top)
- 11:56, 21 September 2010 (diff | hist) N UTDallas/7 September 2010 (New page: {{Template:UTDallasTop}} {{Template:UTDallasMenu}} <span style="color:#EDE8E8"> <html> <style type="text/css"> table, tr, td {background:transparent; border:0px;} table table table {backgr...) (top)
- 02:00, 4 September 2010 (diff | hist) UTDallas/3 September 2010 (→September 3, 2010) (top)
- 01:59, 4 September 2010 (diff | hist) UTDallas/3 September 2010 (→Notebook)
- 01:59, 4 September 2010 (diff | hist) N File:9-3.JPG (top)
- 01:58, 4 September 2010 (diff | hist) UTDallas/2 September 2010 (→September 2, 2010) (top)
- 01:58, 4 September 2010 (diff | hist) UTDallas/2 September 2010 (→September 2, 2010)
- 01:58, 4 September 2010 (diff | hist) UTDallas/2 September 2010
- 01:57, 4 September 2010 (diff | hist) N File:9-2 (2).JPG (top)
- 01:56, 4 September 2010 (diff | hist) N File:9-2.JPG (top)
- 01:55, 4 September 2010 (diff | hist) UTDallas/1 September 2010 (top)
- 01:54, 4 September 2010 (diff | hist) N UTDallas/3 September 2010 (New page: {{Template:UTDallasTop}} {{Template:UTDallasMenu}} <span style="color:#EDE8E8"> <html> <style type="text/css"> table, tr, td {background:transparent; border:0px;} table table table {backgr...)
- 01:53, 4 September 2010 (diff | hist) N UTDallas/2 September 2010 (New page: {{Template:UTDallasTop}} {{Template:UTDallasMenu}} <span style="color:#EDE8E8"> <html> <style type="text/css"> table, tr, td {background:transparent; border:0px;} table table table {backgr...)
- 01:53, 4 September 2010 (diff | hist) N UTDallas/1 September 2010 (New page: {{Template:UTDallasTop}} {{Template:UTDallasMenu}} <span style="color:#EDE8E8"> <html> <style type="text/css"> table, tr, td {background:transparent; border:0px;} table table table {backgr...)
- 01:44, 2 September 2010 (diff | hist) UTDallas/31 August 2010 (→Notebook) (top)
- 01:44, 2 September 2010 (diff | hist) UTDallas/30 August 2010 (→Notebook) (top)
- 01:43, 2 September 2010 (diff | hist) UTDallas/27 August 2010 (→Notebook) (top)
- 01:41, 2 September 2010 (diff | hist) UTDallas/26 August 2010 (→August 26, 2010) (top)
- 01:40, 2 September 2010 (diff | hist) N File:8-26.JPG (top)
- 01:38, 2 September 2010 (diff | hist) UTDallas/24 August 2010 (→August 24, 2010) (top)
- 01:38, 2 September 2010 (diff | hist) N File:8-24.JPG (top)
- 01:37, 2 September 2010 (diff | hist) UTDallas/23 August 2010 (→August 23, 2010) (top)
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