User contributions
From 2010.igem.org
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- 11:05, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/RFPbead tests (→Spectro-photometer data)
- 11:05, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/RFPbead tests (New page: =RFP Beads= Having established our ratio method of signal detection worked well in soil, we next wanted to combine this with our new bead technology to form and test a yet more advanced p...)
- 11:03, 18 October 2010 (diff | hist) N File:GFP RFP in beads.png
- 10:54, 18 October 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/Experiments (→Experiments)
- 10:50, 18 October 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/signal soil (→Signal Visibility in Soil)
- 10:50, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/signal soil (New page: =Signal Visibility in Soil= For this project to work it was essential that a signal could actually be detected when in soil. To test this we mixed 'E. coli' constitutively expressing GFP ...)
- 10:40, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Experiments (→Experiments)
- 10:39, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/characterising ratio (New page: =Characterising the Ratio Method= ==0 - 10mM Assay== Having made our double transformants the next step was to characterise their response to Nitrate. We began by examining this between ...)
- 10:39, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Experiments (→Experiments)
- 10:38, 18 October 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/0-10mM RFPtest (→0 - 10mM Assay) (top)
- 10:37, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/0-10mM RFPtest (→0 - 2mM Assay)
- 10:32, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/0-10mM RFPtest (→Characterising the Ratio Method)
- 10:25, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/0-10mM RFPtest (→Characterising the Ratio Method)
- 10:17, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/0-10mM RFPtest
- 08:31, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/0-10mM RFPtest (New page: Having made our double transformants the next step was to characterise their response to Nitrate. We began by examining this between 0 and 10mM of Potassium Nitrate (in 2mM steps). 5mL sol...)
- 08:14, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/adding RFP (New page: ==Implementing the Ratio Method== To solve the problem of not knowing whether a strong signal was due to many weakly fluorescing bacteria or a few strong ones we decided to implement a ra...) (top)
- 20:13, 17 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/difference solution (New page: ==Bead Difference in Solution== Having made our first beads containing the new biobrick BBa_K381001 we wanted to test whether there was a significant difference in fluorescence between th...)
- 20:12, 17 October 2010 (diff | hist) N File:Bead difference.jpg (top)
- 20:01, 17 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/making beads (→Making Beads)
- 19:58, 17 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/making beads (New page: ==Making Beads== To improve signal detection we decided to encapsulate our bacteria inside beads made of gellan gum and set about developing our own procedure. Research yielded several p...)
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