User contributions
From 2010.igem.org
- 18:34, 26 October 2010 (diff | hist) Team:Tsinghua/support (→Reference)
- 18:33, 26 October 2010 (diff | hist) Team:Tsinghua/support (→Reference)
- 18:30, 26 October 2010 (diff | hist) Team:Tsinghua/team/team (→The Students)
- 18:26, 26 October 2010 (diff | hist) Team:Tsinghua/team/team (→The Students)
- 17:31, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 17:30, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 17:10, 26 October 2010 (diff | hist) Team:Tsinghua/team/team (→The Students)
- 17:09, 26 October 2010 (diff | hist) Team:Tsinghua/team/team (→The Students)
- 17:04, 26 October 2010 (diff | hist) Team:Tsinghua/team/team (→The Students)
- 16:57, 26 October 2010 (diff | hist) N File:THU-沙排-0023.JPG (top)
- 16:40, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 16:39, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 16:37, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 16:36, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 16:33, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 16:16, 26 October 2010 (diff | hist) N File:THU-hdy-6.JPG (top)
- 16:15, 26 October 2010 (diff | hist) N File:THU-hdy-5.JPG (top)
- 16:15, 26 October 2010 (diff | hist) N File:THU-hdy-4.JPG (top)
- 16:14, 26 October 2010 (diff | hist) N File:THU-hdy-3.JPG (top)
- 16:13, 26 October 2010 (diff | hist) N File:THU-hdy-2.JPG (top)
- 16:11, 26 October 2010 (diff | hist) File:THU-hdy-1.JPG (uploaded a new version of "Image:THU-hdy-1.JPG") (top)
- 16:08, 26 October 2010 (diff | hist) N File:THU-hdy-1.JPG
- 12:14, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 12:13, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 12:12, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 12:12, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 12:11, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 12:10, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 12:08, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 12:05, 26 October 2010 (diff | hist) N File:THU-Fan6.JPG (top)
- 12:05, 26 October 2010 (diff | hist) N File:THU-Fan5.JPG (top)
- 12:04, 26 October 2010 (diff | hist) N File:THU-Fan4.JPG (top)
- 12:04, 26 October 2010 (diff | hist) N File:THU-Fan3.JPG (top)
- 11:59, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 11:35, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 11:23, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 08:09, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 08:01, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:59, 26 October 2010 (diff | hist) File:Fan1.JPG (uploaded a new version of "Image:Fan1.JPG") (top)
- 07:57, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:52, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:51, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:35, 26 October 2010 (diff | hist) N File:Fan2.JPG (top)
- 07:30, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:27, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:27, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:25, 26 October 2010 (diff | hist) N File:Fan1.JPG
- 07:21, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:18, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:15, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:06, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:05, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 07:05, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 06:55, 26 October 2010 (diff | hist) Team:Tsinghua/experiments
- 06:56, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/26 August 2010 (→Module I, DT and Fan's part:)
- 06:56, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/25 August 2010 (→Module I, DT and Fan's part:)
- 06:56, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/19 August 2010 (→Module I, DT and Fan's part:)
- 06:56, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/18 August 2010 (→Module I, DT and Fan's part:)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/17 August 2010 (→Module I, DT and Fan's part:)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/16 August 2010 (→Module I, DT and Fan's part:)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/15 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/14 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:55, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/13 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:54, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/12 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:54, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/11 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:54, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/10 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:54, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/9 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:53, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/8 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:53, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/7 August 2010 (→Module I, DT and Fan's part:)
- 06:53, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/6 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:53, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/5 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:52, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/4 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:52, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/3 August 2010 (→Module I, DT and Fan's part:) (top)
- 06:52, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/2 August 2010 (→Module I, DT and Fan's part:)
- 06:51, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/28 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:51, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/27 July 2010 (→Module I, DT and Fan's part:)
- 06:51, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/19 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:50, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/18 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:50, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/15 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:50, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/14 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:50, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/13 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:49, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/11 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:49, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/10 July 2010 (→Module I, DT and Fan's part:) (top)
- 06:48, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/9 July 2010 (→Module I, DT and Fan's part:) (top)
- 10:33, 3 September 2010 (diff | hist) N File:10-8-26.jpg (top)
- 10:32, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/26 August 2010 (New page: == Module I, DT and Fan's part: == Run a colony PCR with universal primers. Amplify the bacteria at the same time. PCR system (SuperMix): H2O 8μl primer1 1μl primer2 1μ...)
- 10:30, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/25 August 2010 (→Module I, DT and Fan's part:)
- 10:29, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/25 August 2010 (New page: == Module I, DT and Fan's part: == Ligate PEMK with C. Using the PEMK①. Double digestion system: chlr: H2O 5μl buffer Tango 2μl chlr 10μl KpnI ...)
- 10:27, 3 September 2010 (diff | hist) N File:10-8-19.jpg (top)
- 10:27, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/19 August 2010 (New page: == Module I, DT and Fan's part: == Use the universal primers to detect. == result == Image:10-8-19.jpg Only the PEM+K 1 and 5 are positive.)
- 10:26, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/18 August 2010 (New page: == Module I, DT and Fan's part: == Pick 6 colonies each from PEM+K and PC+K plate. Amplify and purify the plasmids. Measure the concentration.)
- 10:25, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/17 August 2010 (New page: == Module I, DT and Fan's part: == Ligate again from PEM and PC by adding K fragments. Double digestion system: kan: H2O 9μl buffer BamHI 5μl kan 30μl KpnI ...)
- 10:23, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/16 August 2010 (New page: == Module I, DT and Fan's part: == Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown b...)
- 10:22, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/15 August 2010 (New page: == Module I, DT and Fan's part: == PCR and double digestion to detect. Double digestion system: H2O 8μl buffer tango 4μl plasmid 5μl EcoRI 1μl BamHI ...)
- 10:21, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/14 August 2010 (New page: == Module I, DT and Fan's part: == Pick out 6 colonies to amplify. After 10 hours, purify the plasmids and measure the concentration.)
- 10:19, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/13 August 2010 (New page: == Module I, DT and Fan's part: == Use the PEM② and PKC① to go on the further experiments. Double digestion. Double digestion system: PEM, PKC: H2O 25.5μl buffer tang...)
- 10:18, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/12 August 2010 (→result)
- 10:17, 3 September 2010 (diff | hist) N File:10-8-12.jpg (top)
- 10:16, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/12 August 2010 (→== result ==)
- 10:16, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/12 August 2010 (New page: == Module I, DT and Fan's part: == Use the K-up and K-do to detect the P+K+C. Meanwhile use the digestion protocol to ensure the result. Double digestion system: P+E+M: H2O ...)
- 10:11, 3 September 2010 (diff | hist) N File:10-8-11.jpg (top)
- 10:11, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/11 August 2010 (New page: == Module I, DT and Fan's part: == Pick out 6 colonies to amplify. After 10 hours, purify the plasmids. Use PCR to identify P+E+M (E do & M do primers) and P+K+C (K up & C do primers) wit...)
- 10:08, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/10 August 2010 (→Module I, DT and Fan's part:)
- 10:08, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/10 August 2010 (→Module I, DT and Fan's part:)
- 09:59, 3 September 2010 (diff | hist) N File:10-8-10.jpg (top)
- 09:56, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/10 August 2010 (→Module I, DT and Fan's part:)
- 09:53, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/10 August 2010 (New page: == Module I, DT and Fan's part: == To ensure the result again, we decide to run a PCR with each gene’s own primers. PCR system (SuperMix): H2O 8.5μl primer1 0.5μl pri...)
- 09:51, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/9 August 2010 (→Module I, DT and Fan's part:)
- 09:51, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/9 August 2010 (→Module I, DT and Fan's part:)
- 09:51, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/9 August 2010 (→Module I, DT and Fan's part:)
- 09:50, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/9 August 2010 (New page: == Module I, DT and Fan's part: == Measure the concentration of the plasmids purified last night. Double digest to ensure the positive clone. Double digestion system: P+E: H2O ...)
- 09:48, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/8 August 2010 (New page: == Module I, DT and Fan's part: == Using the 0.1% Amp LB to amplify the selected E.coli at 37℃ for at least 10h. For P+E and P+C, each one selects six colonies. Purify the plasmid 10 ...)
- 09:47, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/7 August 2010 (→Module I, DT and Fan's part:)
- 09:46, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/7 August 2010 (→Module I, DT and Fan's part:)
- 09:44, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/7 August 2010 (New page: == Module I, DT and Fan's part: == Double digestion of the eGFP, chlr and pUC19 for each pair of restriction sites. Digest at 37℃. Double digestion system: eGFP: H2O 2μl ...)
- 09:39, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/6 August 2010 (New page: == Module I, DT and Fan's part: == Get the pUC19 plasmid. Transfer the pUC19, pKD13 and pKD3 plasmids to trans5α seperetly and amplify. Purify the pUC19, pKD13 and pKD3 plasmids at night...)
- 09:37, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/5 August 2010 (New page: == Module I, DT and Fan's part: == pBMN-PIB plasmid purification. == result == No colony with pKD13 or pKD3 exists. It is said that these two plasmids also have the Amp resistant gene. ...)
- 09:34, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/4 August 2010
- 09:32, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/3 August 2010 (New page: == Module I, DT and Fan's part: == Amplify the plasmids pBMN-PIB, pKD13 and pKD3 with competent cells trans5α. All use 0.1% Amp to select.)
- 09:31, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/2 August 2010 (New page: == Module I, DT and Fan's part: == Change the plasmid for part I. pUC19 is suitable for most of the restriction sites and it is highly copied inside the E. coli. Thus primers 1-N-up (or d...)
- 09:28, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/28 July 2010 (New page: == Module I, DT and Fan's part: == Amplify the M, K and C. Wait for the plasmid.)
- 09:27, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/27 July 2010 (New page: == Module I, DT and Fan's part: == Using the newly synthesized primers go through a pre-test experiment. PCR system (FastPFU): H2O 31.4μl 5×buffer 10μl dNTP 5μ...)
- 09:26, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/19 July 2010
- 09:21, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/18 July 2010 (New page: == Module I, DT and Fan's part: == Change the program of the PCR without receiving a positive result…)
- 09:20, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/9 July 2010 (→Module I, DT and Fan's part:)
- 09:17, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/14 July 2010 (→Module I, DT and Fan's part:)
- 09:16, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/14 July 2010 (→Module I, DT and Fan's part:)
- 09:15, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/15 July 2010 (New page: == Module I, DT and Fan's part: == Ligate E (short for eGFP, the same below) and M (short for mCherry), K (short for Kan) and C (short for Chlr) first at 25℃ for 0.5h. Ligation system:...)
- 09:12, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/14 July 2010 (→Module I, DT and Fan's part:)
- 09:11, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/14 July 2010 (→Module I, DT and Fan's part:)
- 09:09, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/14 July 2010 (→Module I, DT and Fan's part:)
- 09:07, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/14 July 2010 (New page: == Module I, DT and Fan's part: == Measure the concentration of the PCR products. Gene Concentration(μg/ml) OD 260/280 eGFP 95.9 1.73 mCherry 61.2 1...)
- 09:03, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/13 July 2010 (New page: == Module I, DT and Fan's part: == PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragmen...)
- 09:02, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/11 July 2010 (New page: == Module I, DT and Fan's part: == Detect the concentration of the four digestion products. (Only the Chlr enjoys an acceptable concentration, we cannot go on our ligation today.) Repea...)
- 09:00, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/10 July 2010 (→Module I, DT and Fan's part:)
- 08:59, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/10 July 2010 (New page: == Module I, DT and Fan's part: == Digest the four fragments with restriction enzyme separately except the SalI and EcoRI at 37℃ for more than 3h. Ligate the four fragments at 25℃ fo...)
- 08:52, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/9 July 2010 (→Module I, DT and Fan's part:)
- 08:51, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/9 July 2010 (→Module I, DT and Fan's part:)
- 08:46, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/9 July 2010 (→Module I, DT and Fan's part:)
- 08:44, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/9 July 2010 (→Module I, DT and Fan's part:)
- 08:35, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/9 July 2010 (→Module I, DT and Fan's part:)
- 08:30, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/9 July 2010 (New page: == Module I, DT and Fan's part: == PCR the fragments of the eGFP, mCherry, Kan and Chlr gene within the pBMN-PIB, pBS34, pKD13 and pKD3 plasmids. The primers using are shown as follow: <...)