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- 03:56, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling (top)
- 03:55, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 03:50, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 03:37, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/22 October 2010 (→Ligation protocol) (top)
- 03:35, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 03:33, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 03:31, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 03:29, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 03:29, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 03:28, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 03:15, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 03:13, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Project (→Results) (top)
- 03:06, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Safety (→Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?) (top)
- 03:05, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Safety (/* Is there a local biosafety group, committee, or review board at your institution? * If yes, what does your local biosafety group think about your project? * If no, which specific biosafety rules or guidelines do you have to consider in y)
- 03:05, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Safety (→Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, * did you document these issues in the Registry? * How did you manage to handle the safety issue?)
- 03:04, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Safety (→Would any of your project ideas raise safety issues in terms of: * researcher safety, * public safety, or * environmental safety?)
- 02:04, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/25 October 2010 (→“THE BLUE GUY”) (top)
- 02:02, 28 October 2010 (diff | hist) N File:Team-IvyTech-South Bend notes.png (top)
- 01:58, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/22 October 2010 (→Ligation protocol)
- 01:52, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/22 October 2010 (→Ligation protocol)
- 01:50, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/22 October 2010 (→Ligation protocol)
- 01:35, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/22 October 2010 (→Ligation protocol)
- 01:32, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/22 October 2010 (→Electroporation of DNA (LuxR) and (LacZ))
- 01:12, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/21 October 2010 (→Electrophoresis Setup:) (top)
- 00:51, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/21 September 2010 (→9/21/10) (top)
- 00:48, 28 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/21 October 2010 (→9/21/10)
- 00:44, 28 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/21/10) (top)
- 19:14, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/20 October 2010 (→Enzyme Parts) (top)
- 19:14, 27 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/20 October 2010 (New page: ==10/20/10== Results for plating Plate B showed a white colony growth Plates A, C, D have shown no signs of growth for 1 day Keeping all plateed in 37C incubator for additional day J...)
- 19:08, 27 October 2010 (diff | hist) N File:Image-IvyTech-South Bend DIGEST.png (top)
- 18:49, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/19 October 2010 (→Using electrocom cells/DNA for electroporateion) (top)
- 18:48, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/19 October 2010 (→New restriction and Ligation)
- 18:31, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/19 October 2010 (→10/19/10)
- 18:16, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/17/10)
- 18:14, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/15 October 2010 (→10/15/10) (top)
- 18:13, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/15 October 2010 (→more 10/15)
- 18:13, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→10/15/10)
- 17:51, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/14 October 2010 (→10/14/10) (top)
- 17:45, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/14 October 2010
- 17:44, 27 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/14 October 2010 (New page: https://static.igem.org/mediawiki/2010/9/95/Image-IvyTech-South_Bend_Assemble.png)
- 17:43, 27 October 2010 (diff | hist) N File:Image-IvyTech-South Bend Assemble.png (top)
- 17:14, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 17:13, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 17:12, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 17:02, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→10/12/10)
- 17:01, 27 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/13 October 2010 (New page: ==10/13/10== Discoveries! T-9002-Agro + E.coli sup >3days> glowing >gram (-) -Possibilities E.coli sucked in T9002 to become E.coliT9002 -Possibilities Agro T9002 became gram(-) prese...) (top)
- 16:55, 27 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/6 October 2010 (New page: == 10/6/10== LB Agar plating and Bacteria streaking 1. I’m starting off this day by heating LB agar, to pur lates for Streaking -I put the LB agar in the microwave put setting on “be...) (top)
- 16:48, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/5 October 2010 (→10/5/10) (top)
- 16:41, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/5 October 2010 (→I13511)
- 15:20, 27 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/26 October 2010 (New page: == 10/26/10== Yesterday was a huge discovery. E.coli does not produce AHL so we were testing the wrong bacteria. Pseudomonas produce AHL so we belive that a contamination happened to produ...) (top)
- 14:14, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (top)
- 14:14, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team
- 14:13, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team
- 14:11, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Who we are)
- 14:09, 27 October 2010 (diff | hist) File:IvyTech-South Bend Team Rachelle.png (uploaded a new version of "Image:IvyTech-South Bend Team Rachelle.png") (top)
- 14:09, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Who we are)
- 14:08, 27 October 2010 (diff | hist) N File:IvyTech-South Bend Team Joe1.png (top)
- 14:07, 27 October 2010 (diff | hist) File:IvyTech-South Bend Team Joe.png (uploaded a new version of "Image:IvyTech-South Bend Team Joe.png") (top)
- 14:06, 27 October 2010 (diff | hist) File:IvyTech-South Bend Team Joe.png (uploaded a new version of "Image:IvyTech-South Bend Team Joe.png")
- 14:05, 27 October 2010 (diff | hist) File:IvyTech-South Bend Team Joe.png (uploaded a new version of "Image:IvyTech-South Bend Team Joe.png": Reverted to version as of 21:29, 7 July 2010)
- 14:04, 27 October 2010 (diff | hist) File:IvyTech-South Bend Team Joe.png (uploaded a new version of "Image:IvyTech-South Bend Team Joe.png")
- 13:59, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Who we are)
- 13:58, 27 October 2010 (diff | hist) N File:IvyTech-South Bend Irene.jpg (top)
- 13:53, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Where we're from)
- 13:52, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Where we're from)
- 13:51, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Where we're from)
- 13:50, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Where we're from)
- 13:50, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team
- 13:43, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Where we're from)
- 13:42, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Team (→What we did)
- 13:36, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Project (→Results)
- 13:34, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Project (→Results)
- 13:32, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→10/26/10)
- 13:31, 27 October 2010 (diff | hist) N File:IvyTech-South Bend team.png (top)
- 13:27, 27 October 2010 (diff | hist) N File:IvyTech-South Bend team.jpg (top)
- 13:22, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→10/26/10)
- 13:22, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→10/25/10)
- 13:21, 27 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→10/21/10)
- 13:19, 27 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/25 October 2010 (New page: == “THE BLUE GUY”== Today we will parity DNA from BlueGuy found on Friday. Also will do an assay testing our part against purified AHL, and pulling and ligating our Clonphenol backbo...)
- 13:05, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/1 October 2010 (→9/24/10) (top)
- 13:04, 27 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/1 October 2010 (→Electroporation Comp E-Coli w/PGlo)
- 21:56, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/24 October 2010 (→9/24/10) (top)
- 21:55, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/24 October 2010 (→9/24/10)
- 21:54, 26 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/24 October 2010 (New page: ==9/24/10== https://static.igem.org/mediawiki/2010/e/ec/TeamIvyTech-South_Bend_DGproc.PNG 1-pull sample of 500 ul of A. Tumefaciens , T9002 and A. Tumefaciens only w/ sterile loop and suspend...)
- 21:39, 26 October 2010 (diff | hist) N File:TeamIvyTech-South Bend DGproc.PNG (top)
- 21:32, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/23 October 2010 (→10/23/10) (top)
- 21:32, 26 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/23 October 2010 (New page: ==10/23/10== “moving on” Completing steps w/A.tumefaciens and supernants D) I’m going to pull 500 ul of my A.Tumefaciens and add 500 ul LB broth and put in a steril tube.(total 100ul...)
- 19:39, 26 October 2010 (diff | hist) Team:IvyTech-South Bend/Parts (top)
- 19:38, 26 October 2010 (diff | hist) Team:IvyTech-South Bend/Parts
- 19:37, 26 October 2010 (diff | hist) N File:IvyTechSBend-BBa K498000.png (top)
- 19:30, 26 October 2010 (diff | hist) Team:IvyTech-South Bend/Parts
- 19:29, 26 October 2010 (diff | hist) Team:IvyTech-South Bend/Parts
- 19:27, 26 October 2010 (diff | hist) Team:IvyTech-South Bend/Parts
- 19:09, 26 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/22 October 2010 (New page: == 9/22/10== Hit it all with AHL Scarape baterian the resuspend in 1 ml lb. (bacteria was grown by jh) Cuvettes A_ 200 ul of bacteria + 800 ul of lb B_ 200 ul of bacteria + 800 ul of A. t...)
- 18:49, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/21 October 2010 (→9/21/10)
- 18:48, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/21 October 2010
- 14:56, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/19 October 2010 (→B-Galactosidase Activity Assay -- Marian Price-Carter, 9/7/00)
- 14:55, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/19 October 2010
- 14:37, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/19 October 2010 (→B-Galactosidase Activity Assay -- Marian Price-Carter, 9/7/00)
- 14:34, 26 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/19 October 2010 (New page: == B-Galactosidase Activity Assay -- Marian Price-Carter, 9/7/00 == Modified From Protocols Online 10/15/10 (htt p://rothlab.ucdavis.ed u/proto cols/beta-galactosidase-3 .html) Day 1: St...)
- 14:30, 26 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→10/15/10)
- 14:30, 26 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/30/10)
- 14:28, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/15 October 2010 (→B-galactosidase Assay)
- 14:17, 26 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/28/10)
- 14:16, 26 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/28/10)
- 14:14, 26 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/15 October 2010 (→B-galactosidase Assay)
- 19:41, 21 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/15/10)
- 19:40, 21 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/15 October 2010 (New page: Using openwetware.org protocol we wil be using restriction enzymes to peice biobrick T9002 purified DNA with LacZ pruified DNA. Cutting LacZ @ or with SP and T9002. a) + E,X creating EX...)
- 19:12, 21 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/12 October 2010 (→10/12/10) (top)
- 19:04, 21 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/21 October 2010 (New page: == Preparation of Electrocompetent Cells == See Ausubel et al. (1987) and Miller and Nickoloff (1995) for additional information. 1. Inoculate 500 ml of L-broth with 1/100 volume of a f...)
- 18:58, 21 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→10/21/10)
- 18:57, 21 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→10/19/10)
- 18:20, 19 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/28/10)
- 17:54, 19 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→IGEM Part #s and Uses)
- 21:03, 17 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/12 October 2010 (New page: == 10/12/10 == Using openwetware.org protocol we wil be using restriction enzymes to peice biobrick T9002 purified DNA with LacZ pruified DNA. Cutting LacZ @ or with SP and T9002. a) ...)
- 20:55, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/8 October 2010 (→Electroporation) (top)
- 20:49, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/8 October 2010 (→1:00)
- 20:35, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/8 October 2010
- 20:34, 17 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/8 October 2010 (New page: == 10/8/10 == Making 4 1L bottlse of LB-Lennox Broth 2- with Agar 2- without I placed between 10.035 - 10.005 grams into each of the 4 bottles, then added 500 ml 18 ohm H2O into each...)
- 20:23, 17 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/7 October 2010 (New page: == 10/7/2010 == Electroporating 2 parts with E-coli bacteria. Parts I13521 and I 13522 will follow Electroporation protocol I13521 - kv 1.8 ms 5.5 X 2 I13522 - kv 1.8 ms 5.5 X 2 Afte...) (top)
- 20:11, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/5 October 2010 (→I13511)
- 20:11, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/5 October 2010 (→I13511)
- 20:10, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/5 October 2010 (→pTet GFP)
- 19:56, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/1 October 2010 (→Electroporation Comp E-Coli w/PGlo)
- 19:55, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/1 October 2010 (→Electroporations Comp E-Coli w/PGlo)
- 19:52, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/1 October 2010
- 19:51, 17 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/1 October 2010 (New page: We are going to gram stain the agrobacteria, E-Coli, and florescent spl. and efflorescent spl. of agro to determine if we have agro that we are working with. Hun Young is stetting up this...)
- 19:13, 17 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/30 September 2010 (→9/30/10) (top)
- 19:07, 17 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 18:53, 17 October 2010 (diff | hist) Team:IvyTech-South Bend/Project (→Machines Used for Experiments)
- 18:52, 17 October 2010 (diff | hist) Team:IvyTech-South Bend/Project (→Machines Used for Experiments)
- 18:40, 17 October 2010 (diff | hist) Team:IvyTech-South Bend/Project (→Overall project)
- 18:25, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/30 September 2010 (New page: ==9/30/10== Today we have been looking at the IGEM registry to find a LacZ without GFP also with RBS and terminator and with the same assembly standard. I found a part ; BBa_I732017 we ha...)
- 18:12, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/1 July 2010 (New page: == GRAM STAIN PROTOCOL == 1. With a wax pencil draw a series of circles about tbe size of dime on a new glass slide and numbeithem "’I, 2, 3.4, 5, 6. 2. Prepare bacteria for staining ...) (top)
- 18:10, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/29 June 2010 (New page: == '''Electroporation'''== Pipette the DNA samples (5 ng - 2 ~tg in a volume - 3 p.l) to be electroporated into sterile 1.5 ml microfuge tubes. Thaw the competent cells at room temperatu...) (top)
- 15:14, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→6/28/10)
- 15:13, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→7/28/10)
- 15:12, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/30 June 2010 (New page: == Richard Lab:Electroporation of E. coli == This protocol is for the typical electro-transformation of E. coli done in the Richard Lab. The consensus electroporation protocol should be...) (top)
- 15:11, 12 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/28 June 2010 (top)
- 15:08, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 15:07, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook
- 15:04, 12 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/28 July 2010 (top)
- 15:03, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/28 July 2010 (New page: Usage and extraction Usage The Distribution Kit Plates contain dried DNA of hundreds of parts that are available in the Registry up to 2009. While there is not enough DNA for assembly, y...)
- 15:00, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:59, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook
- 14:57, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:56, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:55, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:52, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:47, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:45, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:44, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:41, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/6 November 2010 (New page: == !!!JAMBOREE TIME!!! ==) (top)
- 14:38, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/28 June 2010 (New page: == Richard Lab:Electroporation of E. coli == This protocol is for the typical electro-transformation of E. coli done in the Richard Lab. The consensus electroporation protocol should b...)
- 14:34, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:18, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook
- 14:09, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/17/10)
- 14:08, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/17 September 2010 (New page: Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work! Following protocols from pgs...) (top)
- 14:06, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/16/10)
- 14:05, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/16 September 2010 (New page: Today we will wash our electrocomp grown t/pos bacteria with 1.2 mL Glycerol then electroporate Following protocol from page 15 First prof. T sterilized the glycerol passing it through...) (top)
- 13:49, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/21 September 2010 (New page: == 9/21/10 == Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23 1) I will put frozen electro comp cells from -80 degrees C freezer 2) We wil...)
- 13:48, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/21/10)
- 13:48, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/22 September 2010 (New page: == 9/22/10 == Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample 1) Scrap bacteria from transformed plate...) (top)
- 13:44, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/22/10)
- 13:42, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/22/10)
- 13:41, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/23/10)
- 13:41, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/24/10)
- 13:40, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/24 September 2010 (New page: Today I’m testing absorbance of our trials @ 395nm 1) .733 @ 395nm .678 @ 395nm 2) .855 @ 395nm .586 @ 395nm 3) .892 @ 395nm .693 @ 395nm 4) .708 @ 395nm .733 @ 395nm 5) .700 @...) (top)
- 13:37, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook
- 13:36, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/23 September 2010 (New page: == 9/23/10 == Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces. 1) I took and set up new growing Cultures...) (top)
- 13:33, 12 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/28/10)
- 13:32, 12 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/28 September 2010 (New page: Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10 pg 26 1) we will pick 2 colonies from each plate and transfer 15 mL LB – Len...) (top)
- 19:59, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/15/10)
- 19:58, 7 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/15 September 2010 (New page: == 9/15/10 == - Today we will be extracting DNA from out electroporated cells/T9002 - Wash our grown pos. bacteria with 10 percent glycerol to create Electro comp. cells - Then Electrop...) (top)
- 19:57, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/15/10)
- 19:57, 7 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/14 September 2010 (New page: == 9/14/2010 == Today we found growth from our Electroporated E – Coli parts now we took 10 mL made by GM 4/10/10 and poured 10 mL into 3 sterile tubes then added 10 mL Amp to each t...) (top)
- 19:56, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/14/10)
- 19:55, 7 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/10 September 2010 (→9/10/10) (top)
- 19:53, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/10/10)
- 19:51, 7 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/10 September 2010 (New page: == 9/10/10 == Lux Casette Right Promoter BBa_I1051 (2008) plate C002 well 3 – C, 2007, Plate 1 18P, 2006 DNA – 1 18 – P PLux/C1 Hybrid promoter BBa_K091107 – well 8 – P plate ...)
- 19:47, 7 October 2010 (diff | hist) Team:IvyTech-South Bend
- 19:46, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Project
- 19:46, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Parts
- 19:45, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Modeling
- 19:44, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook
- 19:43, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Safety
- 19:43, 7 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend (top)
- 19:42, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Team
- 19:41, 7 October 2010 (diff | hist) N File:Image-SBModeling.PNG (top)
- 19:40, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Team
- 19:40, 7 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend
- 19:39, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Safety
- 19:39, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook
- 19:38, 7 October 2010 (diff | hist) m Team:IvyTech-South Bend/Modeling
- 19:37, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Parts
- 19:37, 7 October 2010 (diff | hist) Team:IvyTech-South Bend
- 19:35, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Project
- 19:33, 7 October 2010 (diff | hist) N File:Image- SBProject.PNG (top)
- 19:33, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Project
- 19:32, 7 October 2010 (diff | hist) Team:IvyTech-South Bend/Project
- 19:21, 7 October 2010 (diff | hist) File:Modeling.png (uploaded a new version of "Image:Modeling.png": Reverted to version as of 20:22, 23 June 2010) (top)
- 19:20, 7 October 2010 (diff | hist) File:Modeling.png (uploaded a new version of "Image:Modeling.png")
- 19:18, 7 October 2010 (diff | hist) File:Project.png (uploaded a new version of "Image:Project.png") (top)
- 17:55, 5 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/5 October 2010
- 17:55, 5 October 2010 (diff | hist) N Team:IvyTech-South Bend/5 October 2010 (New page: == pTet GFP == Spring 2010 Distribution 2010 Kit Plate 2 8A pSB1A2) (top)
- 17:53, 5 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/5 October 2010 (New page: pTet GFP Untagged GFP behind a constitutive promoter. Usage and Biology GFP only control Spring 2010 Distribution 2010 Kit Plate 2 8A pSB1A2)
- 17:22, 5 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/9/10)
- 17:21, 5 October 2010 (diff | hist) Talk:Team:IvyTech-South Bend/9 September 2010 (top)
- 17:20, 5 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/9 September 2010 (New page: == 9/9/10 == Due to conflict we will be changing from E Coli to yeast. Today we will electroporate the 2 parts we discovered yesterday parts #’s BBa_F2620 a pops receiver and # BBa_T9...)
- 17:17, 5 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/3/10)
- 17:16, 5 October 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/3 September 2010 (New page: == 9/3/10 == Today I’m finishing making the LB/Agar from yesterday. 1) I took the 2 Elenmyier Flasks from hot water bath and melting in microwave. 2) Microwaving on beverage setting o...) (top)
- 17:14, 5 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/2/10)
- 17:14, 5 October 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/2/10)
- 16:57, 5 October 2010 (diff | hist) Team:IvyTech-South Bend
- 16:34, 5 October 2010 (diff | hist) Team:IvyTech-South Bend
- 16:28, 5 October 2010 (diff | hist) Team:IvyTech-South Bend
- 16:27, 5 October 2010 (diff | hist) Team:IvyTech-South Bend
- 19:15, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/2/10)
- 19:05, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/2 September 2010 (→9/2/10) (top)
- 19:04, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/2 September 2010 (→9/2/10)
- 19:02, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/2 September 2010 (→9/2/10)
- 18:56, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/24 August 2010 (→8/24/10) (top)
- 18:50, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 18:49, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 18:48, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook
- 18:46, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/2 September 2010 (→9/2/10)
- 18:46, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/2 September 2010 (→9/2/10)
- 18:46, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/2 September 2010 (→9/2/10)
- 18:45, 30 September 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/2 September 2010 (New page: == 9/2/10 == Results from streaking – Transformed DNA grew on all four plates and progressively grew less when the Amp Con. was raised. So selection was possibly made. We will run a El...)
- 18:42, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→9/1/10)
- 18:41, 30 September 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/1 September 2010 (New page: == 9/1/10 == Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock. Fisher Bio Reagents...) (top)
- 18:39, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→8/31/10)
- 18:39, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook
- 18:37, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→8/31/10)
- 18:37, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→8/27/10)
- 18:36, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→8/27/10)
- 18:34, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/31 August 2010 (→8/31/10) (top)
- 18:33, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/31 August 2010 (→8/31/10)
- 18:33, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/31 August 2010 (→8/31/10)
- 18:31, 30 September 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/31 August 2010 (New page: == 8/31/10 == Results of transformation - the plates had growth but (no) distinct colony pattern. The amp that was added to the Agar seemed to have been destroyed before plates were pour...)
- 18:30, 30 September 2010 (diff | hist) Talk:Team:IvyTech-South Bend/27 August 2010 (→8/27/10) (top)
- 18:29, 30 September 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/27 August 2010 (New page: == 8/27/10 == We will be running a gel to determine if our DNA sample was successfully electroplated. 1) In microwave I melted TAE buffer/gel on the beverage setting and let melt the c...)
- 18:27, 30 September 2010 (diff | hist) N Talk:Team:IvyTech-South Bend/24 August 2010 (New page: == 8/24/10 == Today we will be pouring new plates for streaking new transformed cells. 1) I located some 2% Ara/agur made by Greg M. on 4/10/10. 2) Place the 250 ml Elemyier flask in m...)
- 18:26, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→8/24/10)
- 18:25, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook
- 18:19, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→8/24/10)
- 18:18, 30 September 2010 (diff | hist) Team:IvyTech-South Bend/Notebook (→Notebook)
- 14:18, 29 September 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Where we're from)
- 14:15, 29 September 2010 (diff | hist) N File:IvyTech-South Bend Team Location.png (top)
- 14:14, 29 September 2010 (diff | hist) Team:IvyTech-South Bend/Team (→Where we're from)
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