User contributions
From 2010.igem.org
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- 20:59, 27 October 2010 (diff | hist) SMG17MC (top)
- 20:58, 27 October 2010 (diff | hist) N GSM17- Agar (New page: '''GSM17- Agar''' - M17-Agar - 0.5 M Sucrose - 0.5% Glucose) (top)
- 20:56, 27 October 2010 (diff | hist) N SMG17MC (New page: '''SMG17MC''' -M17 -o.5 M Sucrose -0.5 % Glucose -20 mM MgCl2 -2 mM CaCl2)
- 20:54, 27 October 2010 (diff | hist) Team:Groningen/Protocols for Lactococcus (top)
- 20:53, 27 October 2010 (diff | hist) N Wash buffer (New page: '''Wash buffer''' -0.5 M Sucrose (17g/100ml) -10% gylcerol( 11.5ml/100 ml)) (top)
- 20:51, 27 October 2010 (diff | hist) N SMGG (New page: '''SMGG''' -M17 -0.5 M Sucrose -0.5% Glucose -1% Glycine) (top)
- 20:49, 27 October 2010 (diff | hist) N Team:Groningen/Protocols for Lactococcus (New page: '''Preparation of cells''' *10 ml ON culture (SMGG) in 100 ml SMGG *Grow to OD600=0.2-0.7 *Wash three times with 50 ml icecold wash buffer*Resuspend in 1 ml wash buffer '''Electr...)
- 19:56, 27 October 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 16:19, 25 October 2010 (diff | hist) Team:Groningen/Protocols for Streptomyces (top)
- 15:44, 25 October 2010 (diff | hist) N Team:Groningen/Antibiotics and Concentrationas (New page: '''Antibiotics''' Ampicillin 100 mg/ml Ampicillin (1000x) Stock *1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH) *Add NaOH or KOH to allow the Ampicillin to dissol...) (top)
- 15:44, 25 October 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 15:42, 25 October 2010 (diff | hist) m Team:Groningen/Antibiotics Concentrationas and Organisms (top)
- 15:23, 25 October 2010 (diff | hist) Team:Groningen/Antibiotics Concentrationas and Organisms
- 17:22, 9 September 2010 (diff | hist) Team:Groningen/Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer (top)
- 17:22, 9 September 2010 (diff | hist) N Team:Groningen/Protocols for Bacillus subtilis 168 (New page: '''2-step transformation procedure for ''Bacillus subtilis'' 168''' *Cells are grown O/N at 37 °C in supplemented medium contains Spizizen's salts (without antibiotics) *Dilute c...) (top)
- 17:20, 9 September 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 17:20, 9 September 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 17:19, 9 September 2010 (diff | hist) Team:Groningen/Growing Streptomyces (top)
- 17:19, 9 September 2010 (diff | hist) N Team:Groningen/Antibiotics Concentrationas and Organisms (New page: '''Concentrations for antibiotics ''' '''Antibiotic''' ''' Stock''' ''' E.coli ''' ''' B.subtilis''' *Ampicillin (Amp) (in H2O) 50 mg/ml (4 °C...)
- 17:15, 9 September 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
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