User contributions
From 2010.igem.org
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- 08:45, 24 October 2010 (diff | hist) N Minnesota-experimental/13 October 2010 (New page: '''Matt''' Sent J23105 and J23118 into pSBLC3 plasmids for sequencing at University of Minnesota BioMedical Genomics Center.) (top)
- 08:44, 24 October 2010 (diff | hist) N Minnesota-experimental/11 October 2010 (New page: '''Matt''' Preparations of transformations for FACS protein assay.) (top)
- 08:43, 24 October 2010 (diff | hist) N Minnesota-experimental/8 October 2010 (New page: '''Matt''' Ran gel to confirm digestion band. Transformation of J23105 and J23118 into Top 10 competency cells. Fifty microliters of each plated onto CM plates at thirty seven degree ove...) (top)
- 08:42, 24 October 2010 (diff | hist) N Minnesota-experimental/7 October 2010 (New page: '''Ian''' Digestion reactions of pSBK3 pLac GFP. PCR confirms DNA band sizes.) (top)
- 08:40, 24 October 2010 (diff | hist) N Minnesota-experimental/6 October 2010 (New page: '''Matt''' Minipreps of J23105 and J23118 after cell cultures. Given to Poonam for transformations. Digestion reactions for confirmation with EcoRI/PstI. Placed in thirty degree incubat...) (top)
- 08:38, 24 October 2010 (diff | hist) N Minnesota-experimental/4 October 2010 (New page: '''Ian''' Minipreps of psBLC3 eut SK. Digestion reactions of pSBLC3 with EcoRI/PstI. PCR reactions of pSBLC3 eut SK. pSBLC3 pLac GFP sent for sequencing to University of Minnesota BioMe...) (top)
- 08:35, 24 October 2010 (diff | hist) Minnesota-experimental/2 October 2010 (top)
- 08:35, 24 October 2010 (diff | hist) N Minnesota-experimental/2 October 2010 (New page: '''Matt''' Re-do minipreps of J23105 and J23118 promoters.)
- 08:34, 24 October 2010 (diff | hist) N Minnesota-experimental/1 October 2010 (New page: '''Ian''' Minipreps of Eut SK insert. Ligation reactions of insert into PsBLC3. Transformation reactions of PsBLC3 Eut SK. Stored overnight at thirty seven degrees Celsius incubator.) (top)
- 08:33, 24 October 2010 (diff | hist) N Minnesota-experimental/30 September 2010 (New page: '''Matt''' Mininpreps of overnight culture colonies. Digestion of minipreps with EcoRI/PstI. Overnight in thirty seven degree incubator. '''Tony''' Inoculated JM109 cultures in liquid LB...)
- 08:31, 24 October 2010 (diff | hist) N Minnesota-experimental/29 September 2010 (New page: '''Tony''' No colonies observed. Second attempt of transformations from 9/28. '''Matt''' Transformations of J23105 and J23118 promoters.) (top)
- 08:30, 24 October 2010 (diff | hist) N Minnesota-experimental/28 September 2010 (New page: '''Tony''' Transformations of pucBB EutS, MN, LK, MNLK, SMNLK and pacBB GFP.) (top)
- 08:29, 24 October 2010 (diff | hist) Minnesota-experimental/27 September 2010 (top)
- 08:28, 24 October 2010 (diff | hist) N Minnesota-experimental/27 September 2010 (New page: '''Matt''' PCR of 23102 and 23116 promoters.)
- 08:27, 24 October 2010 (diff | hist) N Minnesota-experimental/22 September 2010 (New page: '''Ian''' Digestion reaction failed. Attempted with PCR. Also ligation reaction with Eut SK into PsBLC3.) (top)
- 08:23, 24 October 2010 (diff | hist) N Minnesota-experimental/21 September 2010 (New page: '''Ian''' Minipreps of psBLC3 Eut SK overnight cultures. Digestion raction of psBLC3 Eut SK with EcoRI/PstI. '''Matt''' Digestion reaction of J23102 and J23116 with EcoRI/SpeI. Placed in...) (top)
- 08:19, 24 October 2010 (diff | hist) N Minnesota-experimental/20 September 2010 (New page: '''Matt''' PCR reaction of J23102 and J23116 promoters. Gel confirms correct DNA band size. .) (top)
- 08:16, 24 October 2010 (diff | hist) Minnesota-experimental/17 September 2010 (top)
- 08:15, 24 October 2010 (diff | hist) N Minnesota-experimental/17 September 2010 (New page: '''Matt''' Performed minipreps of J23102 and J23116 plasmids.)
- 08:14, 24 October 2010 (diff | hist) N Minnesota-experimental/16 September 2010 (New page: '''Tony''' S, M, N, L, K digestions with BglII/NotI and EcoRI/PstI. Ligation of S, M, N, L, K into PsBLC3 linearized plasmid.) (top)
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