User contributions
From 2010.igem.org
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- 03:58, 28 October 2010 (diff | hist) Team:Groningen/For the parents (→Synthetic biology) (top)
- 03:53, 28 October 2010 (diff | hist) Team:Groningen/Expression (→Timed expression of chaplins in a biofilm)
- 03:52, 28 October 2010 (diff | hist) N ExtractionCellWallsGR (New page: Duisrupt your cells using a French press style cell disruptor, keep all collecting parts cool to reduce protease activity. <br> Repeat 6x, pressure: 13 kPSI <br> Spin off, 5 minutes at...) (top)
- 03:44, 28 October 2010 (diff | hist) Team:Groningen/Applications (→Applications) (top)
- 03:42, 28 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 03:39, 28 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 03:38, 28 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 03:35, 28 October 2010 (diff | hist) N File:ShipGR.jpg (top)
- 03:34, 28 October 2010 (diff | hist) N File:Medicgr.jpg (top)
- 03:32, 28 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 03:19, 28 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 02:29, 28 October 2010 (diff | hist) Team:Groningen/25 October 2010 (top)
- 02:22, 28 October 2010 (diff | hist) Team:Groningen/4 October 2010 (top)
- 02:21, 28 October 2010 (diff | hist) N File:MassSpecGR.jpg (top)
- 02:20, 28 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 02:02, 28 October 2010 (diff | hist) Team:Groningen/4 October 2010
- 01:31, 28 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 01:20, 28 October 2010 (diff | hist) File:Groningen-Reel-Stage3.jpg (uploaded a new version of "Image:Groningen-Reel-Stage3.jpg": Reverted to version as of 22:44, 24 October 2010) (top)
- 01:19, 28 October 2010 (diff | hist) File:Groningen-Reel-Stage3.jpg (uploaded a new version of "Image:Groningen-Reel-Stage3.jpg")
- 01:17, 28 October 2010 (diff | hist) Team:Groningen/Biofilm (→Biofilm)
- 00:34, 28 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 00:33, 28 October 2010 (diff | hist) Team:Groningen/Expression
- 00:29, 28 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 00:27, 28 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 00:25, 28 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 00:23, 28 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:56, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:49, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:48, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:47, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:47, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:46, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:45, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:44, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Timed expression of chaplins in a biofilm)
- 23:43, 27 October 2010 (diff | hist) N File:MassSpec.jpg (top)
- 23:41, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:33, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:23, 27 October 2010 (diff | hist) N File:Chgr.jpg (top)
- 23:19, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:15, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:12, 27 October 2010 (diff | hist) N File:Egr.jpg (top)
- 23:09, 27 October 2010 (diff | hist) N File:EHgr.jpg (top)
- 23:08, 27 October 2010 (diff | hist) N File:Cgr.jpg (top)
- 23:04, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 23:02, 27 October 2010 (diff | hist) Team:Groningen/Protocols (→Protocols) (top)
- 21:57, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 21:44, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 21:43, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 21:42, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 21:41, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Subtilin induced expression of chaplins)
- 21:36, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Expression of chaplins)
- 20:38, 27 October 2010 (diff | hist) Team:Groningen/Expression (→Expression of chaplins)
- 19:48, 27 October 2010 (diff | hist) Team:Groningen/Applications (→Hydrophobic coatings)
- 02:33, 27 October 2010 (diff | hist) Team:Groningen/Applications (→Biofilm coatings)
- 02:27, 27 October 2010 (diff | hist) Team:Groningen/Applications (→Anti-fouling coatings)
- 02:17, 27 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 02:15, 27 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 02:14, 27 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 02:10, 27 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 02:08, 27 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 02:01, 27 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 22:42, 24 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 22:15, 24 October 2010 (diff | hist) Team:Groningen/Applications (→Applications)
- 22:03, 24 October 2010 (diff | hist) Team:Groningen/Applications (→Applications of biofilms)
- 17:16, 24 October 2010 (diff | hist) N File:THTGR4f.jpg (top)
- 17:15, 24 October 2010 (diff | hist) N File:THTGR4e.jpg (top)
- 17:13, 24 October 2010 (diff | hist) N File:THTGR4d.jpg (top)
- 17:13, 24 October 2010 (diff | hist) N File:THTGR4c.jpg (top)
- 17:12, 24 October 2010 (diff | hist) N File:THTGR4b.jpg (top)
- 17:11, 24 October 2010 (diff | hist) N File:THTGR4.jpg (top)
- 17:10, 24 October 2010 (diff | hist) N File:THTGR5.jpg (top)
- 17:08, 24 October 2010 (diff | hist) N File:THTGR6e.jpg (top)
- 17:08, 24 October 2010 (diff | hist) N File:THTGR6d.jpg (top)
- 17:06, 24 October 2010 (diff | hist) N File:THTGR6c.jpg (top)
- 17:05, 24 October 2010 (diff | hist) N File:THTGR6b.jpg (top)
- 17:05, 24 October 2010 (diff | hist) N File:THTGR6.jpg (top)
- 17:03, 24 October 2010 (diff | hist) N File:GrowthCurveGR2b.jpg (top)
- 17:02, 24 October 2010 (diff | hist) N File:GrowthCurveGR2.jpg (top)
- 17:02, 24 October 2010 (diff | hist) N File:GrowthCurveGR3.jpg (top)
- 17:01, 24 October 2010 (diff | hist) N File:GrowthCurveGR4.jpg (top)
- 17:00, 24 October 2010 (diff | hist) N File:GrowthCurveGR5.jpg (top)
- 16:58, 24 October 2010 (diff | hist) N File:GrowthCurveGR6.jpg (top)
- 16:55, 24 October 2010 (diff | hist) N SDS-PAGEGR (New page: '''Normal SDS-PAGE using TRIS-Glycine gels and electrphoresis buffer.''' <br> Wear vinyl gloves at all times! <br> Per gel take one small glass plate etc.) (top)
- 16:54, 24 October 2010 (diff | hist) N THTstainingGR (New page: '''THT staining''' <br> Thioflavin T is a stain that specifically binds to amyloid fibres. Since chaplins assemble into amyloids, this is a detection method worth trying. From literature...) (top)
- 16:49, 24 October 2010 (diff | hist) N LysozymePrepGR (New page: '''Lysozyme preperation''' <br> Add lysozyme, 10 mg/mL. <br> Incubate for 30 minutes at 60 degrees Celsius. <br> Freeze dry the whole sample.) (top)
- 16:47, 24 October 2010 (diff | hist) N SupernatantPrepGR (New page: '''TCA precipitation - Medium fraction''' <br> Spin down your sample asap, collect the supernatant by pipetting carefully. Pre-cool 100% TCA and 80% aceton on ice. <br> Add 100 uL of p...) (top)
- 16:38, 24 October 2010 (diff | hist) N TFAtreatmentGR (New page: '''TFA treatment''' <br> - Make sure samples a completely dried out. Add 1ml 99% extra pure TFA per max 50 mg of sample. <br> - Then vortex TFA with sample for 1 min <br> - Then spin do...) (top)
- 16:36, 24 October 2010 (diff | hist) N PelletPrepGR (New page: '''Pellet preperation, chaplin expression test''' Spin down your samples, discard the supernatant (or use it SupernatantPrepGR). Wash your pellet 3x Use the TFA treatment to extract...) (top)
- 16:20, 24 October 2010 (diff | hist) Team:Groningen/20 September 2010
- 16:17, 24 October 2010 (diff | hist) Team:Groningen/6 September 2010
- 16:16, 24 October 2010 (diff | hist) Team:Groningen/13 September 2010
- 16:14, 24 October 2010 (diff | hist) Team:Groningen/13 September 2010
- 16:13, 24 October 2010 (diff | hist) Team:Groningen/23 August 2010
- 16:11, 24 October 2010 (diff | hist) Team:Groningen/23 August 2010
- 16:11, 24 October 2010 (diff | hist) Team:Groningen/23 August 2010
- 16:10, 24 October 2010 (diff | hist) Team:Groningen/16 August 2010
- 16:09, 24 October 2010 (diff | hist) Team:Groningen/16 August 2010
- 16:03, 24 October 2010 (diff | hist) Team:Groningen/16 August 2010
- 16:01, 24 October 2010 (diff | hist) Team:Groningen/16 August 2010
- 15:58, 24 October 2010 (diff | hist) Team:Groningen/16 August 2010
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