User contributions
From 2010.igem.org
- 10:52, 26 October 2010 (diff | hist) Induction protocols for GAL1 CUP1 MET17 promoters (top)
- 14:09, 25 October 2010 (diff | hist) Galactose dose response of Gal1 Promoter in pRS415 (Removing all content from page) (top)
- 14:08, 25 October 2010 (diff | hist) N Team:Aberdeen Scotland/Galactose dose response of Gal1 Promoter in pRS415 (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <h1>Measurement of dose responsiveness of the GAL1 promoter to galactose using construct GAL1p-(Npep-GFP)</h1> <h3>Aim</h3...)
- 14:08, 25 October 2010 (diff | hist) Team:Aberdeen Scotland/Results
- 14:05, 25 October 2010 (diff | hist) Timed Induction of Gal1 Promoter in pRS415 (Removing all content from page) (top)
- 14:04, 25 October 2010 (diff | hist) N Team:Aberdeen Scotland/Timed Induction of Gal1 Promoter in pRS415 (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <h1>Measurement of induction of the GAL1 promoter over time in construct GAL1p-(Npep-GFP)</h1> <h3>Aim</h3> <p>The aim of ...)
- 14:04, 25 October 2010 (diff | hist) Team:Aberdeen Scotland/Results
- 13:59, 25 October 2010 (diff | hist) Copper Dose Response of the CUP1 Promoter Using N4 (Removing all content from page) (top)
- 13:59, 25 October 2010 (diff | hist) N Team:Aberdeen Scotland/Copper Dose Response of the CUP1 Promoter Using N4 (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <h1>Quantitative Determination of the Response of the CUP1p - [GFP] to Varying Concentration of CuSO4</h1> <h3>Aim...)
- 13:58, 25 October 2010 (diff | hist) Team:Aberdeen Scotland/Results
- 13:50, 25 October 2010 (diff | hist) Timed Induction of the CUP1 Promoter Using N4 (Removing all content from page) (top)
- 13:49, 25 October 2010 (diff | hist) N Team:Aberdeen Scotland/Timed Induction of the CUP1 Promoter Using N4 (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <h1>Timed Induction of GFP Expression by the CUP1p-[GFP]</h1> <h3>Aim</h3> <p>The aim of this experiment is to det...)
- 13:49, 25 October 2010 (diff | hist) Team:Aberdeen Scotland/Results
- 13:35, 25 October 2010 (diff | hist) Galactose dose response of Gal1 Promoter in pRS415
- 13:34, 25 October 2010 (diff | hist) Timed Induction of Gal1 Promoter in pRS415
- 13:07, 24 October 2010 (diff | hist) MS2 Coat-Protein Effect on Expression of GFP in pRS415
- 13:01, 24 October 2010 (diff | hist) N File:MS2vsGFP FACS 2.jpg (top)
- 12:58, 24 October 2010 (diff | hist) N File:MS2vsGFP FACS 1.jpg (top)
- 12:57, 24 October 2010 (diff | hist) MS2 Coat-Protein Effect on Expression of GFP in pRS415
- 12:56, 24 October 2010 (diff | hist) N File:MS2vsGFP fluorimeter.jpg (top)
- 12:55, 24 October 2010 (diff | hist) N File:MS2vsGFP fluorescent cells.jpg (top)
- 12:54, 24 October 2010 (diff | hist) MS2 Coat-Protein Effect on Expression of GFP in pRS415
- 12:50, 24 October 2010 (diff | hist) N File:Conditions for FACS analysis of MS2vsGFP.jpg (top)
- 12:44, 24 October 2010 (diff | hist) MS2 Coat-Protein Effect on Expression of GFP in pRS415
- 11:34, 24 October 2010 (diff | hist) Team:Aberdeen Scotland/Switch Characterisation
- 11:28, 24 October 2010 (diff | hist) Team:Aberdeen Scotland/Biobrick Related
- 11:26, 24 October 2010 (diff | hist) Team:Aberdeen Scotland/Biobrick Related
- 11:19, 24 October 2010 (diff | hist) Team:Aberdeen Scotland/Biobrick Related
- 11:05, 24 October 2010 (diff | hist) Team:Aberdeen Scotland/Biobrick Related
- 10:52, 24 October 2010 (diff | hist) Team:Aberdeen Scotland/Switch Characterisation
- 10:50, 24 October 2010 (diff | hist) Switch Characterisation (Removing all content from page) (top)
- 10:49, 24 October 2010 (diff | hist) N Switch Characterisation (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <h2>Prep Week (7th – 11th of June)</h2><br> 1.Confirmation of successfully integrated cassettes at the Trp1 locus of:<b...)
- 10:26, 24 October 2010 (diff | hist) Team:Aberdeen Scotland/Header
- 10:23, 24 October 2010 (diff | hist) Team:Aberdeen Scotland/Header
- 10:21, 24 October 2010 (diff | hist) Team:Aberdeen Scotland/Header
- 18:56, 8 October 2010 (diff | hist) MS2 coat-protein effect on expression of GFP in pRS415 (top)
- 18:55, 8 October 2010 (diff | hist) N File:Fluorimeter graph for Ms2 repression.jpg (top)
- 18:53, 8 October 2010 (diff | hist) N MS2 coat-protein effect on expression of GFP in pRS415 (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <h1>Characterisation of the inhibition of GFP expression in pRS415 through the binding of the MS2 coat protein to ...)
- 18:52, 8 October 2010 (diff | hist) N File:GFP fluo under MS2 repression.jpg (top)
- 18:48, 8 October 2010 (diff | hist) N File:Setup for MS2 charac.jpg (top)
- 18:43, 8 October 2010 (diff | hist) Team:Aberdeen Scotland/Results
- 18:42, 8 October 2010 (diff | hist) Team:Aberdeen Scotland/TeamMembers
- 18:41, 8 October 2010 (diff | hist) N File:Me.jpg (top)
- 18:27, 8 October 2010 (diff | hist) Copper Dose Response of the Cup1 promoter using N4
- 18:25, 8 October 2010 (diff | hist) 4. Replacing the CUP1 promoter in pRS414 with the CUP1-2 promoter from the N4 construct
- 18:24, 8 October 2010 (diff | hist) N File:Absence of CFP fluoresence.jpg (top)
- 18:22, 8 October 2010 (diff | hist) N File:PRS414 construct.jpg (top)
- 18:13, 8 October 2010 (diff | hist) 3. N-GFP Swap Experiment
- 18:12, 8 October 2010 (diff | hist) 3. N-GFP Swap Experiment
- 18:12, 8 October 2010 (diff | hist) N File:Fluor graph for N-GFP swap.jpg (top)
- 18:10, 8 October 2010 (diff | hist) N File:N-GFP swap construct.jpg (top)
- 18:10, 8 October 2010 (diff | hist) N File:Joint diagram of pRS414+pRS415.jpg (top)
- 15:46, 8 October 2010 (diff | hist) 2. Confirming the CFP sequence is functional
- 15:45, 8 October 2010 (diff | hist) N File:Evidence of CFP fluo.jpg (top)
- 15:43, 8 October 2010 (diff | hist) N File:Construct generated to test CFP.jpg (top)
- 15:38, 8 October 2010 (diff | hist) N File:Diagram of YCP lac 22 Fl.jpg (top)
- 15:34, 8 October 2010 (diff | hist) Experimental Layout
- 15:32, 8 October 2010 (diff | hist) N File:Diagram of pRS414.jpg (top)
- 15:30, 8 October 2010 (diff | hist) Copper Dose Response of the Cup1 promoter using N4
- 15:28, 8 October 2010 (diff | hist) N File:Dose response of pCUP1.jpg (top)
- 15:25, 8 October 2010 (diff | hist) Timed Induction of the Cup1 promoter using N4
- 15:24, 8 October 2010 (diff | hist) File:PCUP1 response graph.jpeg (uploaded a new version of "Image:PCUP1 response graph.jpeg") (top)
- 15:15, 8 October 2010 (diff | hist) N File:PCUP1 response graph.jpeg
- 14:54, 8 October 2010 (diff | hist) Copper Dose Response of the Cup1 promoter using N4
- 14:48, 8 October 2010 (diff | hist) Timed Induction of the Cup1 promoter using N4
- 14:42, 8 October 2010 (diff | hist) N 4. Replacing the CUP1 promoter in pRS414 with the CUP1-2 promoter from the N4 construct (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <h1>Checking the copper promoter in pRS414 by replacing it with the CUP1-2 promoter from the N4 construct</h1> <h3...)
- 14:39, 8 October 2010 (diff | hist) Experimental Layout
- 14:37, 8 October 2010 (diff | hist) N 3. N-GFP Swap Experiment (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <h1>The N-GFP Swap Experiment</h1> <h3>Aim</h3> <p>The aim of this experiment is to see if the pRS414 construct po...)
- 14:33, 8 October 2010 (diff | hist) N 2. Confirming the CFP sequence is functional (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <h1>Confirming the CFP sequence is functional</h1> <h3>Aim</h3> <p>As part of the experiments to repair the pRS414...)
- 14:26, 8 October 2010 (diff | hist) 1. Confirmation using microscope and fluorometer analysis that the pRS414 construct was not expressing CFP
- 14:25, 8 October 2010 (diff | hist) 1. Confirmation using microscope and fluorometer analysis that the pRS414 construct was not expressing CFP
- 14:24, 8 October 2010 (diff | hist) Experimental Layout
- 14:21, 8 October 2010 (diff | hist) N 1. Confirmation using microscope and fluorometer analysis that the pRS414 construct was not expressing CFP (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <h1>Confirmation using microscope and fluorometer analysis that pRS414 was not expressing CFP</h1> <h3>Aim</h3> <p...)
- 14:20, 8 October 2010 (diff | hist) N Experimental Layout (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <h1>Troubleshooting pRS414</h1> <h3>Aim</h3> <p>Following the analysis of the two promoters using N4 and N25 the ne...)
- 13:58, 13 September 2010 (diff | hist) N Experiemtal Layout (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <head> <meta http-equiv=Content-Type content="text/html; charset=windows-1252"> <meta name=Generator content="Micr...) (top)
- 13:54, 13 September 2010 (diff | hist) N Timed Induction of the Cup1 promoter using N4 (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <head> <meta http-equiv=Content-Type content="text/html; charset=windows-1252"> <meta name=Generator content="Micr...)
- 13:50, 13 September 2010 (diff | hist) Copper Dose Response of the Cup1 promoter using N4
- 13:48, 13 September 2010 (diff | hist) Copper Dose Response of the Cup1 promoter using N4
- 13:46, 13 September 2010 (diff | hist) N Copper Dose Response of the Cup1 promoter using N4 (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> <head> <meta http-equiv=Content-Type content="text/html; charset=windows-1252"> <meta name=Generator content="Micr...)
- 13:33, 13 September 2010 (diff | hist) Team:Aberdeen Scotland/Results
- 13:18, 13 September 2010 (diff | hist) Team:Aberdeen Scotland/Results
- 13:56, 23 August 2010 (diff | hist) Team:Aberdeen Scotland/Constructs
- 12:24, 19 August 2010 (diff | hist) N Team:Aberdeen Scotland/Modelling (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> </html>) (top)
- 12:24, 19 August 2010 (diff | hist) N Team:Aberdeen Scotland/BioBricks (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> </html>) (top)
- 12:24, 19 August 2010 (diff | hist) N Team:Aberdeen Scotland/Toggle Switch (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> </html>) (top)
- 12:23, 19 August 2010 (diff | hist) Team:Aberdeen Scotland/Header
- 11:28, 19 August 2010 (diff | hist) N Team:Aberdeen Scotland/General Overview (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> </html>)
- 11:26, 19 August 2010 (diff | hist) N Team:Aberdeen Scotland/Main Experiments (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> </html>)
- 11:26, 19 August 2010 (diff | hist) N Team:Aberdeen Scotland/Equipment (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> </html>) (top)
- 11:25, 19 August 2010 (diff | hist) N Team:Aberdeen Scotland/Results (New page: {{:Team:Aberdeen_Scotland/css}} {{:Team:Aberdeen_Scotland/Title}} <html> </html>)
- 11:24, 19 August 2010 (diff | hist) Team:Aberdeen Scotland/Header
- 11:21, 19 August 2010 (diff | hist) Team:Aberdeen Scotland/Header