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2024-03-29T14:47:05Z
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http://2010.igem.org/Team:Queens-Canada/4_August_2010
Team:Queens-Canada/4 August 2010
2010-10-28T01:19:17Z
<p>ThaiPhi: </p>
<hr />
<div><h2>August 4, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Basia and Thai<br />
<br />
Used 0.3 g agarose in 45 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao and Basia<br />
<br />
Re-performed heat shock from [[Team:Queens-Canada/3 August 2010|03/08/10]] with leftover TOP10 cells and additional 5 µL of ligation product. Prepared new LB plates with Chlor antibiotics.<br />
Before plating, tubes were spun down at 13000g for 2 mins, the supernatant was discarded, and pellets were resuspended in 200µL of 2XTY.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Hao and Thai<br />
<br />
'''pStr-1'''::1A2, '''unc-54 3'UTR'''::1A3, '''pRab-7'''::1A3<br />
<br />
HB Buffer was not used (not crucial).<br />
<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Thai and Hao<br />
<br />
'''pHsp-3''', '''pSra-10'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 33 s and annealing temperature of 59 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Thai and Mary<br />
<br />
Cut with EcoRI and PstI.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Thai and Mary<br />
<br />
'''pStr-1::1A2''', '''unc-54 3'UTR::1A3''', '''pRab-7::1A3'''<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 110 V.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/5_August_2010
Team:Queens-Canada/5 August 2010
2010-10-28T01:17:56Z
<p>ThaiPhi: </p>
<hr />
<div><h2>August 5, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Thai and Basia<br />
<br />
'''pMec-7''', '''pStr-1'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C. <br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Basia and Thai<br />
<br />
'''pStr-1''', '''pMec-7''', '''pStr-1::1A2''', '''pSra-10''', '''pHsp-3'''<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Chris<br />
<br />
'''pRab-7''', '''pSip-1''', '''pStr-1''', '''pHsp-3'''<br />
<br />
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 110 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Chris<br />
<br />
'''pSra-10''', '''pHsp-3'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/6_August_2010
Team:Queens-Canada/6 August 2010
2010-10-28T01:15:56Z
<p>ThaiPhi: </p>
<hr />
<div><h2>August 6, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao<br />
<br />
'''pSra-10''', '''pHsp-3'''<br />
<br />
Used 0.3 g agarose in 40 mL TBE. Gel run for 50 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Hao and Thai<br />
<br />
'''ChR2''', '''eCFP''', '''eYFP''', '''mCherry'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 15 s and annealing temperature of 61 °C.<br />
<br />
'''mCherry''' was run for 5 cycles without primers, then for 35 cycles with primers.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Hao and Steve and Thai<br />
<br />
'''NpHR''', '''pOdr-10''', '''eYFP''', '''pStr-220''', '''mCherry''', '''pOsm-10''', '''pOdr-1''', '''eCFP''', '''pMec-7''', '''pFlp-1''', '''pCeh-12''', '''pIC518''', '''ChR2''', '''pSrb-6''', '''pSB1C3'''<br />
<br />
All inserts ligated into '''pSB1C3'''. T4 ligase used.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao and Thai<br />
<br />
Ligation products from [[Team:Queens-Canada/6 August 2010|06/08/10]]<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Thai<br />
<br />
'''eYFP''','''ChR2''', '''mCherry'''<br />
<br />
Used 0.3 g agarose in 45 mL TBE. Gel run for 45 mins at 100 V.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/6_August_2010
Team:Queens-Canada/6 August 2010
2010-10-28T01:15:35Z
<p>ThaiPhi: </p>
<hr />
<div><h2>August 6, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao<br />
<br />
'''pSra-10''', '''pHsp-3'''<br />
<br />
Used 0.3 g agarose in 40 mL TBE. Gel run for 50 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Hao and Thai<br />
<br />
'''ChR2''', '''eCFP''', '''eYFP''', '''mCherry'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 15 s and annealing temperature of 61 °C.<br />
<br />
'''mCherry''' was run for 5 cycles without primers, then for 35 cycles with primers.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Hao and Steve and Thai<br />
<br />
'''NpHR''', '''pOdr-10''', '''eYFP''', '''pStr-220''', '''mCherry''', '''pOsm-10''', '''pOdr-1''', '''eCFP''', '''pMec-7''', '''pFlp-1''', '''pCeh-12''', '''pIC518''', '''ChR2''', '''pSrb-6''', '''pSB1C3'''<br />
<br />
All inserts ligated into '''pSB1C3'''. T4 ligase used.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao and Thai<br />
Ligation products from [[Team:Queens-Canada/6 August 2010|06/08/10]]<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Thai<br />
'''eYFP''','''ChR2''', '''mCherry'''<br />
<br />
Used 0.3 g agarose in 45 mL TBE. Gel run for 45 mins at 100 V.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/8_July_2010
Team:Queens-Canada/8 July 2010
2010-10-28T01:13:11Z
<p>ThaiPhi: </p>
<hr />
<div><h2>July 8, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Thai<br />
<br />
'''pGpd-2''', '''pFlp-1'''<br />
<br />
Program run using HIFI with an extension time of 25 s and annealing temperature of 57 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Chris<br />
<br />
'''pBluescriptII'''<br />
<br />
Plates stored in 37 °C room overnight.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Thai and Yuli<br />
<br />
Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Chris<br />
<br />
Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli<br />
<br />
'''BBa_I13522'''<br />
<br />
Cut using EcoRI and PstI.<br />
<br />
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao and Yuli<br />
<br />
Practiced injections with water.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/28_June_2010
Team:Queens-Canada/28 June 2010
2010-10-28T01:11:12Z
<p>ThaiPhi: </p>
<hr />
<div><h2>June 28, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Steve<br />
<br />
'''pSip-1''', '''pMec-7''', '''pRab-7'''<br />
<br />
For '''pSip-1''' and '''pRab-7''':<br />
Program run using HIFI with an extension time of 12 s and annealing temperature of 58 °C.<br />
<br />
For '''pMec-7''':<br />
Program run using HIFI with an extension time of 30 s and annealing temperature of 56 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Basia and Yuli<br />
<br />
'''pSip-1''':::'''eGFP''' Extract, '''pRab-7''':::'''eGFP''' Extract, '''pMec-7''':::'''eGFP''' Extract, '''unc-54 3'UTR'''<br />
<br />
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao and Yuli<br />
<br />
Practiced injections with water.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/25_June_2010
Team:Queens-Canada/25 June 2010
2010-10-28T01:09:43Z
<p>ThaiPhi: </p>
<hr />
<div><h2>June 25, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Hao and Thai<br />
<br />
'''pOdr-1''', '''pOdr-10''', '''pSrb-6''', '''pFlp-1'''<br />
<br />
Program run using TAQ with an extension time of 35 s and annealing temperature of 57 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Hao and Thai<br />
<br />
'''pSra-10''', '''pCeh-12''', '''pHsp-3'''<br />
<br />
Program run using TAQ with an extension time of 55 s and annealing temperature of 58 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]</h3><br />
Thai<br />
<br />
'''pIC576''', '''pIC517''', '''pIC518'''<br />
<br />
Used 250µL of 80% glycerol with 750 µL of culture.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Thai<br />
<br />
'''pStr-220''', '''pStr-1''', '''pOsm-10''', '''pOdr-1''', '''pOdr-10''', '''pSrb-6''', '''pFlp-1''', '''pSra-10''', '''pCeh-12''', '''pHsp-3'''<br />
<br />
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Yuli<br />
<br />
'''unc-54 3'UTR'''<br />
<br />
Program run using HIFI with an extension time of 30 s and annealing temperature of 63 °C.<br />
<br />
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao and Yuli<br />
<br />
Practiced injections with water.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/23_June_2010
Team:Queens-Canada/23 June 2010
2010-10-28T01:09:07Z
<p>ThaiPhi: </p>
<hr />
<div><h2>June 23, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Steve and Yuli<br />
<br />
'''pHsp-3'''<br />
<br />
Program run using HIFI with an extension time of 45 s and annealing temperature of 58 °C.<br />
<br />
2010 <br />
PCR<br />
Hao, Louis and Mary<br />
<br />
'''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-7'''::'''eGFP'''<br />
<br />
Program run using HIFI with an extension time of 30 s and annealing temperature of 57 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Nelson<br />
<br />
'''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-7'''::'''eGFP''', '''pHsp-3'''::'''eGFP'''<br />
<br />
Used 0.4 g agarose in 65 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao and Thai<br />
<br />
'''pIC576''', '''pIC517''', '''pIC518'''<br />
<br />
Plates stored in 37 °C room overnight.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#DNA Rehydration|DNA Rehydration]]</h3><br />
Hao and Thai<br />
<br />
'''BBa_I13522'''<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Thai, Mike and Mary<br />
<br />
'''BBa_I13522'''<br />
<br />
Plates stored in 37 °C room overnight.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Thai, Louis and Mike<br />
<br />
'''pHsp-3'''<br />
<br />
Program run using HIFI with an extension time of 90 s and annealing temperature of 58 °C.<br />
<br />
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao and Yuli<br />
<br />
Practiced injections with water.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/21_June_2010
Team:Queens-Canada/21 June 2010
2010-10-28T01:08:26Z
<p>ThaiPhi: </p>
<hr />
<div>{{:Team:Queens-Canada/head}}<br />
<br />
{{:Team:Queens-Canada/head}}<br />
<h2>June 21, 2010</h2><br />
On this day...<br />
<br />
* We got the wiki up and going!<br />
* We ran a crazy gel and cut it up a whole bunch.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao<br />
<br />
'''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-7'''::'''eGFP'''<br />
<br />
Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Extraction|Gel Extraction]]</h3><br />
Yuli and Hao<br />
<br />
'''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-7'''::'''eGFP'''<br />
<br />
Products stored at -20 °C.<br />
<br />
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao and Yuli<br />
<br />
Practiced injections with water.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/18_June_2010
Team:Queens-Canada/18 June 2010
2010-10-28T01:06:51Z
<p>ThaiPhi: </p>
<hr />
<div>{{:Team:Queens-Canada/head}}<br />
<h2>June 18, 2010</h2><br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Hao and Yuli<br />
<br />
'''pSip-1''', '''pHsp-3''', '''pUnc-47''', '''pRab-7''', '''pMec-7''' with '''BBa_I714891'''<br />
<br />
Program run using HIFI with an extension time of 3 min and annealing temperature of 57 °C without primers for 5 cycles.<br />
Primers were then added and program was run with annealing temperature of 1 min 15 s.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Yuli<br />
<br />
'''pSip-1'''::'''eGFP''', '''pHsp-3'''::'''eGFP''', '''pMec-7'''::'''eGFP''', '''pUnc-47'''::'''eGFP''', '''pRab-7'''::'''eGFP'''<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao and Yuli<br />
<br />
Practiced injections with water<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/18_June_2010
Team:Queens-Canada/18 June 2010
2010-10-28T01:06:22Z
<p>ThaiPhi: </p>
<hr />
<div>{{:Team:Queens-Canada/head}}<br />
<h2>June 18, 2010</h2><br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Hao and Yuli<br />
<br />
'''pSip-1''', '''pHsp-3''', '''pUnc-47''', '''pRab-7''', '''pMec-7''' with '''BBa_I714891'''<br />
<br />
Program run using HIFI with an extension time of 3 min and annealing temperature of 57 °C without primers for 5 cycles.<br />
Primers were then added and program was run with annealing temperature of 1 min 15 s.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Yuli<br />
<br />
'''pSip-1'''::'''eGFP''', '''pHsp-3'''::'''eGFP''', '''pMec-7'''::'''eGFP''', '''pUnc-47'''::'''eGFP''', '''pRab-7'''::'''eGFP'''<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao and Yuli<br />
Practiced injections with water<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/30_August_2010
Team:Queens-Canada/30 August 2010
2010-10-28T01:05:13Z
<p>ThaiPhi: </p>
<hr />
<div><h2>August 30, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Thai<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Hao and Thai<br />
<br />
'''pSip-1'''::1C3, '''pFlp-1'''::1C3, '''pOdr-10'''::1C3, '''mCherry'''::1C3<br />
<br />
{| border="1"<br />
| EcoRI and SpeI<br />
| XbaI and PstI<br />
| EcoRI and PstI<br />
|-<br />
| '''pStr-220'''::1C3<br />
| '''eCFP'''::'''unc-54 3'UTR'''::1T3<br />
| '''pSB1T3'''<br />
|-<br />
|mCherry::1C3<br />
| '''unc-54 3'UTR'''::1C3<br />
| <br />
|-<br />
| '''pFlp-1'''::1C3<br />
| <br />
| <br />
|-<br />
| '''pSip-1'''::1C3<br />
| <br />
| <br />
|-<br />
| '''pOdr-10'''::1C3<br />
| <br />
| <br />
|}<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Hao and Thai<br />
<br />
'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3<br />
<br />
{| border="1"<br />
| EcoRI and SpeI<br />
| XbaI and PstI<br />
| EcoRI and PstI<br />
|-<br />
| '''pStr-1'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''pStr-220'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''pFlp-1'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3 <br />
|-<br />
| '''pOdr-10'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''mCherry'''<br />
| '''unc-54 3'UTR'''<br />
| 1T3<br />
|}<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao<br />
<br />
'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3<br />
<br />
Transformed ligation products from [[Team:Queens-Canada/30 August 2010|30/08/10]]<br />
<br />
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao<br />
<br />
'''pMec-7'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
Transferred F1 transgenic worms to new plate<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/30_August_2010
Team:Queens-Canada/30 August 2010
2010-10-28T01:02:32Z
<p>ThaiPhi: </p>
<hr />
<div><h2>August 30, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Thai<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Hao and Thai<br />
<br />
'''pSip-1'''::1C3, '''pFlp-1'''::1C3, '''pOdr-10'''::1C3, '''mCherry'''::1C3<br />
<br />
{| !border="1"<br />
| EcoRI and SpeI<br />
| XbaI and PstI<br />
| EcoRI and PstI<br />
|-<br />
| '''pStr-220'''::1C3<br />
| '''eCFP'''::'''unc-54 3'UTR'''::1T3<br />
| '''pSB1T3'''<br />
|-<br />
|mCherry::1C3<br />
| '''unc-54 3'UTR'''::1C3<br />
| <br />
|-<br />
| '''pFlp-1'''::1C3<br />
| <br />
| <br />
|-<br />
| '''pSip-1'''::1C3<br />
| <br />
| <br />
|-<br />
| '''pOdr-10'''::1C3<br />
| <br />
| <br />
|}<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Hao and Thai<br />
<br />
'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3<br />
<br />
{|<br />
| EcoRI and SpeI<br />
| XbaI and PstI<br />
| EcoRI and PstI<br />
|-<br />
| '''pStr-1'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''pStr-220'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''pFlp-1'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3 <br />
|-<br />
| '''pOdr-10'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''mCherry'''<br />
| '''unc-54 3'UTR'''<br />
| 1T3<br />
|}<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao<br />
<br />
'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3<br />
<br />
Transformed ligation products from [[Team:Queens-Canada/30 August 2010|30/08/10]]<br />
<br />
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao<br />
<br />
'''pMec-7'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
Transferred F1 transgenic worms to new plate<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/11_June_2010
Team:Queens-Canada/11 June 2010
2010-10-28T00:46:04Z
<p>ThaiPhi: New page: <h3>Microinjection</h3> Hao and Yuli Practiced injections with water</p>
<hr />
<div><h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3><br />
Hao and Yuli<br />
Practiced injections with water</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/28_July_2010
Team:Queens-Canada/28 July 2010
2010-10-28T00:35:59Z
<p>ThaiPhi: </p>
<hr />
<div><h2>July 28, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Hao<br />
<br />
'''pSip-1''', '''pRab-7'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 6 s and annealing temperature of 58 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Steve<br />
<br />
Used 1 g agarose in 140 mL TBE. Gel run for 35 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Hao<br />
<br />
'''pSB1A3'''<br />
<br />
Half the samples were cut with EcoRI & SpeI, and the other half were cut with XbaI and PstI.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Steve<br />
<br />
'''pSB1A3'''<br />
<br />
Used 0.5 g agarose in 70 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion-Ligation|Digestion-Ligation]]</h3><br />
Steve and Thai<br />
<br />
'''pRab-7''', '''pSip-1''', '''pMec-7''', '''pSB1A3'''<br />
<br />
Cut with XbaI and PstI. Backbone treated with CIAP.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Hao and Nelson<br />
<br />
'''pSrb-6''', '''pCeh-12''', '''pOdr-10''', '''NpHR''', '''ChR2''', '''pOsm-10''', '''pStr-1''', '''eCFP''', '''pHsp-3''', '''pFlp-1''', '''pSB1A3''', '''pStr-220'''<br />
<br />
Cut with EcoRI and PstI.<br />
May have damaged spin column filter of '''pHsp-3''' during PCR purification.<br />
Transferred flow-through to new spin column.<br />
Old spin column may have trapped the DNA.<br />
Finished PCR purification with both columns.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/parts
Team:Queens-Canada/parts
2010-10-27T18:53:00Z
<p>ThaiPhi: </p>
<hr />
<div>{{:Team:Queens-Canada/head}}<br />
<br />
<h1>WormWorks Parts List</h1><br />
<br />
<html><div class="section"><h2>Regulatory</h2></html><br />
<br />
All but one of the regulatory elements we isolated are promoters, and are described in brief detail below, with more elaborate information on their parts registry pages. The remaining BioBrick is the 3' UTR from ''unc-54'', which is roughly equivalent to a bacterial terminator and performs a number of important regulatory functions in complement to the promoter. All complete ''C. elegans'' constructs must include some form of functional 3' UTR. Promoters were selected based on their utility, strength of expression, and ease of avoiding potentially harmful cutsites.<br />
<br />
<h3>Constitutive</h3><br />
* '''pGpd-2''': ''gpd-2'' is part of the glycolysis pathway. The gpd-2 promoter thus expresses at a very high level—AceView <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?exdb=AceView&db=worm&term=gpd-2&submit=Go">says 43.5 times the average</a></html> for a ''C. elegans'' constitutive gene.<br />
* '''pHsp-3''': HSP-3 is a protein involved in the heat shock response pathway, but it is expressed constitutively. AceView <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?exdb=AceView&db=worm&term=hsp-3&submit=Go">says this is 22.9 times the average</a></html>.<br />
* '''pRab-7''': ''rab-7'' expresses a GTPase involved in endosome trafficking, and expresses at <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?exdb=AceView&db=worm&term=rab-7&submit=Go">4.3 times the average</a></html>.<br />
<br />
<h3>Inducible</h3><br />
<br />
* '''pSip-1''': '''<html><a target="_new" href="http://wormbase.org/db/gene/gene?name=WBGene00004798;class=Gene">sip-1</a></html>''' encodes a member of the heat shock family of proteins. Accoring to AceView, '''sip-1''' is expressed at a level <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?exdb=AceView&db=worm&term=sip-1&submit=Go">17.6 times the average</a></html>, at all levels of development.<br />
<br />
<h3>Tissue-Specific</h3><br />
<br />
* '''pMec-7''':<br />
* '''pOdr-1''':<br />
* '''pStr-1''':<br />
* '''pOsm-10''':<br />
* '''pFlp-1''':<br />
* '''pSra-10''':<br />
* '''pStr-220''':<br />
<br />
<html></div><div class="section"><h2>Reporters</h2></html><br />
<br />
* '''eGFP''':<br />
* '''eCFP''':<br />
* '''eYFP''':<br />
* '''mCherry''':<br />
* ''Constructs'':<br />
<br />
<html></div><div class="section"><h2>Optogenetics Proteins</h2></html><br />
<br />
* '''ChR2''':<br />
* '''NpHR''':<br />
* ''Fusions'': ChR2::eYFP and NpHR::eCFP<br />
<br />
<html></div><div class="section"><h2>Getting the Parts</h2></html><br />
<br />
You can get our special worm parts through the standard iGEM distribution channel: the <html><a href="http://partsregistry.org" target="_new">Parts Registry</a></html>. Click on a part number below to be taken to the relevant description page.<br />
<br />
<html><script type="text/javascript"><br />
function toggle_visibility(id) {<br />
el = document.getElementById(id);<br />
if(el.style.display == "none") {<br />
el.style.display = "block";<br />
} else {<br />
el.style.display = "none";<br />
}<br />
}</script><br />
<div class="box"><br />
<div id="groupparts" style="display: block;"><br />
</html><groupparts>iGEM010 Queens-Canada</groupparts><html><br />
</div><br />
</div><br />
<br />
</div></html><br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/safety
Team:Queens-Canada/safety
2010-10-27T14:10:06Z
<p>ThaiPhi: </p>
<hr />
<div>{{:Team:Queens-Canada/head}}<br />
<br />
<h1>Risk Assessment</h1><br />
Unlike some other nematodes, ''Caenorhabditis elegans'' is fully free-living and functions primarily as a digester of detritus, posing no threat except to the microbes which it eats. The worms are non-pathogenic organisms, and Biosafety level 1 practices are simple and safe; working with ''C. elegans'' carries a very low level of risk. Accordingly, concerns about researcher, public, and environmental safety are minimal. ''C. elegans'' can be safely cultured in a BSL-1 laboratory{{:Team:Queens-Canada/footnote-anchor|1}}, just like bacteria and yeast.<br />
<br />
The engineered ''C. elegans'' do not effect their environments, as our work focuses on proteins with intracellular effects that have no significant catalytic role. Thus, a malfunction in our BioBrick parts would pose minimal threat to humans, laboratory equipment, or other organisms. Further, our alterations do not grant the worms any advantages over the wildtype, making the spread of these alleles unlikely, should engineered worms be released into the environment: in fact, the high-copy extrachromosomal arrays used in microinjection induce stress on the worm and consequently lower its biological fitness.<br />
<br />
In principle, our work does pose some long-term risks in that it aims to make more advanced forms of synthetic biology more readily accessible, as well as providing a chassis that could potentially be used to create more dangerous projects such as catalytic mechanisms. These risks naturally arise from the advancement of the field of synthetic biology, and although they necessitate dilligence and thoughtfulness from the research community as the field develops, they do not represent undue or extraordinary threats.<br />
<br />
By itself, our project poses no threat to its environment greater than that presented by the standard ''E. coli'' chassis—in fact, even less, since nematodes are not capable of horizontal gene transfer and would disseminate engineered genes more slowly.<br />
<br />
<html><div class="section"><h2>Footnotes and Citations</h2></html><br />
{{:Team:Queens-Canada/footnote|1|[http://www.ccac.ca/en/CCAC_Programs/ETCC/Module04/15.html Biosafety Guidelines and Levels of Containment]. Canadian Council on Animal Care. Accessed on 2010-06-22.}}<html></div></html><br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/20_October_2010
Team:Queens-Canada/20 October 2010
2010-10-27T06:25:04Z
<p>ThaiPhi: </p>
<hr />
<div>{{:Team:Queens-Canada/head}}<br />
<h2>October 20, 2010</h2><br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and CHris<br />
<br />
All 1C3 constructs from the previous week<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Chris<br />
<br />
Digestion products from [[Team:Queens-Canada/20 October 2010|20/10/10]]<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Parts Submission|Parts Submission]]</h3><br />
Chris<br />
<br />
Sent in 4x8-tube per tube strips<br />
<br />
{|<br />
|Part<br />
|Date<br />
|-<br />
| '''pMec-7'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''pSra-10'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''pGpd-2'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''pStr-1'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''ChR2'''::'''eYFP'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''mCherry'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pSip-1'''::1C3<br />
| N/A<br />
|-<br />
| '''ChR2'''::1C3<br />
| N/A<br />
|-<br />
| '''pSip-1'''::1C3<br />
| [[Team:Queens-Canada/25 August 2010|25/08/10]]<br />
|-<br />
| '''pOdr-10'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pFlp-1'''::1C3<br />
| [[Team:Queens-Canada/25 August 2010|25/08/10]]<br />
|-<br />
| '''pHsp-3'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''pOsm-10'''::1C3<br />
| N/A<br />
|-<br />
| '''pOdr-1'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''NpHR'''::'''eCFP'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''eGFP'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pStr-220'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''eYFP'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''NpHR'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''eCFP'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''pStr-220'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pGpd-2'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pSip-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''mCherry'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|}<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/20_October_2010
Team:Queens-Canada/20 October 2010
2010-10-27T06:24:45Z
<p>ThaiPhi: </p>
<hr />
<div><h2>October 20, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and CHris<br />
<br />
All 1C3 constructs from the previous week<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Chris<br />
<br />
Digestion products from [[Team:Queens-Canada/20 October 2010|20/10/10]]<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Parts Submission|Parts Submission]]</h3><br />
Chris<br />
<br />
Sent in 4x8-tube per tube strips<br />
<br />
{|<br />
|Part<br />
|Date<br />
|-<br />
| '''pMec-7'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''pSra-10'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''pGpd-2'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''pStr-1'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''ChR2'''::'''eYFP'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''mCherry'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pSip-1'''::1C3<br />
| N/A<br />
|-<br />
| '''ChR2'''::1C3<br />
| N/A<br />
|-<br />
| '''pSip-1'''::1C3<br />
| [[Team:Queens-Canada/25 August 2010|25/08/10]]<br />
|-<br />
| '''pOdr-10'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pFlp-1'''::1C3<br />
| [[Team:Queens-Canada/25 August 2010|25/08/10]]<br />
|-<br />
| '''pHsp-3'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''pOsm-10'''::1C3<br />
| N/A<br />
|-<br />
| '''pOdr-1'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''NpHR'''::'''eCFP'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''eGFP'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pStr-220'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''eYFP'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''NpHR'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''eCFP'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''pStr-220'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pGpd-2'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pSip-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''mCherry'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|}<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/20_October_2010
Team:Queens-Canada/20 October 2010
2010-10-27T06:23:58Z
<p>ThaiPhi: </p>
<hr />
<div><h2>October 20, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and CHris<br />
<br />
All 1C3 constructs from the last week<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Chris<br />
<br />
Digestion products from [[Team:Queens-Canada/20 October 2010|20/10/10]]<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Parts Submission|Parts Submission]]</h3><br />
Chris<br />
<br />
Sent in 4x8-tube per tube strips<br />
<br />
{|<br />
|Part<br />
|Date<br />
|-<br />
| '''pMec-7'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''pSra-10'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''pGpd-2'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''pStr-1'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''ChR2'''::'''eYFP'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''mCherry'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pSip-1'''::1C3<br />
| N/A<br />
|-<br />
| '''ChR2'''::1C3<br />
| N/A<br />
|-<br />
| '''pSip-1'''::1C3<br />
| [[Team:Queens-Canada/25 August 2010|25/08/10]]<br />
|-<br />
| '''pOdr-10'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pFlp-1'''::1C3<br />
| [[Team:Queens-Canada/25 August 2010|25/08/10]]<br />
|-<br />
| '''pHsp-3'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''pOsm-10'''::1C3<br />
| N/A<br />
|-<br />
| '''pOdr-1'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''NpHR'''::'''eCFP'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''eGFP'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pStr-220'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''eYFP'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''NpHR'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''eCFP'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''pStr-220'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pGpd-2'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pSip-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''mCherry'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|}<br />
<br />
{{:Team:Queens-Canada/head}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/20_October_2010
Team:Queens-Canada/20 October 2010
2010-10-27T06:22:08Z
<p>ThaiPhi: New page: <h2>October 20, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion</h3> Yuli and CHris All 1C3 constructs from the last week <h3>[[Team:Quee...</p>
<hr />
<div><br />
<h2>October 20, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and CHris<br />
<br />
All 1C3 constructs from the last week<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Chris<br />
<br />
Digestion products from [[Team:Queens-Canada/20 October 2010|20/10/10]]<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Parts Submission|Parts Submission]]</h3><br />
Chris<br />
<br />
Sent in 4x8-tube per tube strips<br />
<br />
{|<br />
|Part<br />
|Date<br />
|-<br />
| '''pMec-7'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''pSra-10'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''pGpd-2'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''pStr-1'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''ChR2'''::'''eYFP'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''mCherry'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pSip-1'''::1C3<br />
| N/A<br />
| '''ChR2'''::1C3<br />
| N/A<br />
| '''pSip-1'''::1C3<br />
| [[Team:Queens-Canada/25 August 2010|25/08/10]]<br />
|-<br />
| '''pOdr-10'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pFlp-1'''::1C3<br />
| [[Team:Queens-Canada/25 August 2010|25/08/10]]<br />
|-<br />
| '''pHsp-3'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''pOsm-10'''::1C3<br />
| N/A<br />
| '''pOdr-1'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''NpHR'''::'''eCFP'''::1C3<br />
| [[Team:Queens-Canada/20 August 2010|20/08/10]]<br />
|-<br />
| '''eGFP'''::1C3<br />
| [[Team:Queens-Canada/16 August 2010|16/08/10]]<br />
|-<br />
| '''pStr-220'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''eYFP'''::1C3<br />
| [[Team:Queens-Canada/19 August 2010|19/08/10]]<br />
|-<br />
| '''NpHR'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''eCFP'''::1C3<br />
| [[Team:Queens-Canada/11 August 2010|11/08/10]]<br />
|-<br />
| '''pStr-220'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pGpd-2'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pSip-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/13 October 2010|13/10/10]]<br />
|-<br />
| '''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''mCherry'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|-<br />
| '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
| [[Team:Queens-Canada/15 October 2010|15/10/10]]<br />
|}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/15_October_2010
Team:Queens-Canada/15 October 2010
2010-10-27T06:21:25Z
<p>ThaiPhi: New page: <h2>October 15, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Steve Liquid Culture from [[Team:Queens-Canada/14 October 2010|14/10/10...</p>
<hr />
<div><br />
<h2>October 15, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Steve<br />
<br />
Liquid Culture from [[Team:Queens-Canada/14 October 2010|14/10/10]]<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Steve<br />
<br />
'''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/14_October_2010
Team:Queens-Canada/14 October 2010
2010-10-27T06:21:04Z
<p>ThaiPhi: </p>
<hr />
<div><h2>October 14, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Steve<br />
<br />
'''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team:Queens-Canada/7 October 2010|07/10/10]]<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Steve<br />
<br />
'''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3, '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''eYFP'''::'''unc-54 3'UTR'''::1C3, '''mCherry'''::'''unc-54 3'UTR'''::1C3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion-Ligation|Digestion-Ligation]]</h3><br />
Steve<br />
<br />
'''pGpd-2''', '''pOdr-10''', '''pStr-220''', '''pOdr-10''', '''pSip-1''', '''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Steve<br />
<br />
Ligation products from [[Team:Queens-Canada/14 October 2010|14/10/10]]<br />
<br />
Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/14_October_2010
Team:Queens-Canada/14 October 2010
2010-10-27T06:20:34Z
<p>ThaiPhi: New page: <h2>October 14, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Heat Shock</h3> Steve '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team...</p>
<hr />
<div><br />
<h2>October 14, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Steve<br />
<br />
'''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team:Queens-Canada/07 October 2010|07/10/10]]<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Steve<br />
<br />
'''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3, '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3, '''eYFP'''::'''unc-54 3'UTR'''::1C3, '''mCherry'''::'''unc-54 3'UTR'''::1C3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion-Ligation|Digestion-Ligation]]</h3><br />
Steve<br />
<br />
'''pGpd-2''', '''pOdr-10''', '''pStr-220''', '''pOdr-10''', '''pSip-1''', '''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Steve<br />
Ligation products from [[Team:Queens-Canada/14 October 2010|14/10/10]]<br />
<br />
Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/13_October_2010
Team:Queens-Canada/13 October 2010
2010-10-27T06:20:07Z
<p>ThaiPhi: New page: <h2>October 13, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Chris '''pStr-220''', '''pOdr-10''', '''pGpd-2''', '''pSip-1'''::'''eCF...</p>
<hr />
<div><br />
<h2>October 13, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Chris<br />
<br />
'''pStr-220''', '''pOdr-10''', '''pGpd-2''', '''pSip-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/12_October_2010
Team:Queens-Canada/12 October 2010
2010-10-27T06:19:51Z
<p>ThaiPhi: New page: {{:Team:Queens-Canada/foot}} <h2>October 12, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Yuli '''pStr-220''', '''pOdr-1...</p>
<hr />
<div>{{:Team:Queens-Canada/foot}}<br />
<br />
<h2>October 12, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Yuli<br />
<br />
'''pStr-220''', '''pOdr-10''', '''pGpd-2''', '''pSip-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/9_October_2010
Team:Queens-Canada/9 October 2010
2010-10-27T06:19:27Z
<p>ThaiPhi: </p>
<hr />
<div><h2>October 9, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Yuli<br />
Products from [[Team:Queens-Canada/7 October 2010|07/10/10]].<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/9_October_2010
Team:Queens-Canada/9 October 2010
2010-10-27T06:19:12Z
<p>ThaiPhi: New page: <h2>October 9, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Heat Shock</h3> Yuli Products from 07/10/10. ...</p>
<hr />
<div><br />
<h2>October 9, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Yuli<br />
Products from [[Team:Queens-Canada/07 October 2010|07/10/10]].<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/7_October_2010
Team:Queens-Canada/7 October 2010
2010-10-27T06:18:55Z
<p>ThaiPhi: New page: <h2>October 7, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion-Ligation</h3> Thai and Hao '''eCFP'''::'''unc-54 3'UTR''', '''pOdr...</p>
<hr />
<div><br />
<h2>October 7, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Digestion-Ligation|Digestion-Ligation]]</h3><br />
Thai and Hao<br />
<br />
'''eCFP'''::'''unc-54 3'UTR''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR''', '''pGpd-2'''::'''eCFP'''::'''unc-54 3'UTR''', '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR''', '''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR''', '''ChR2'''::'''eCFP'''::'''unc-54 3'UTR''', '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR''', '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR''', '''eYFP'''::'''eCFP'''::'''unc-54 3'UTR''', '''mCherry'''::'''eCFP'''::'''unc-54 3'UTR''', '''pStr-220'''::'''eCFP'''::'''unc-54 3'UTR''', '''pSip-1'''::'''eCFP'''::'''unc-54 3'UTR''', '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR''', '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR''', '''pSB1C3'''<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/5_October_2010
Team:Queens-Canada/5 October 2010
2010-10-27T06:18:32Z
<p>ThaiPhi: New page: <h2>October 5, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Thai and Yuli '''pSra-10'''::'''NpHR'''::'''eCFP'''::'''unc-...</p>
<hr />
<div><br />
<h2>October 5, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Thai and Yuli<br />
<br />
'''pSra-10'''::'''NpHR'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pStr-220'''::'''NpHR'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pFlp-1'''::'''NpHR'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pOdr-1'''::'''NpHR'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pOdr-10'''::'''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/4_October_2010
Team:Queens-Canada/4 October 2010
2010-10-27T06:18:20Z
<p>ThaiPhi: </p>
<hr />
<div><h2>October 4, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Yuli and Steve<br />
<br />
'''ChR2'''::'''eYFP'''::'''unc-54 3'UTR''', '''NpHR'''::'''eCFP'''::'''unc-54 3'UTR''', '''pSB1A3'''<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Yuli and Steve<br />
<br />
Ligation products from [[Team:Queens-Canada/4 October 2010|04/10/10]]<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/4_October_2010
Team:Queens-Canada/4 October 2010
2010-10-27T06:17:53Z
<p>ThaiPhi: New page: <h2>October 4, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Ligation</h3> Yuli and Steve '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR''', '''NpHR'''::'...</p>
<hr />
<div><br />
<h2>October 4, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Yuli and Steve<br />
<br />
'''ChR2'''::'''eYFP'''::'''unc-54 3'UTR''', '''NpHR'''::'''eCFP'''::'''unc-54 3'UTR''', '''pSB1A3'''<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Yuli and Steve<br />
Ligation products from [[Team:Queens-Canada/04 October 2010|04/10/10]]<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/1_October_2010
Team:Queens-Canada/1 October 2010
2010-10-27T06:17:33Z
<p>ThaiPhi: New page: <h2>October 1, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion</h3> Yuli and Mary '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1T3, '''NpHR...</p>
<hr />
<div><br />
<h2>October 1, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and Mary<br />
<br />
'''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1T3, '''NpHR'''::'''eCFP'''::'''unc-54 3'UTR'''::1T3<br />
<br />
Cut with XbaI and PstI.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/29_September_2010
Team:Queens-Canada/29 September 2010
2010-10-27T06:17:04Z
<p>ThaiPhi: New page: <h2>September 29, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion</h3> Chris and Basia '''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB...</p>
<hr />
<div><h2>September 29, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Chris and Basia<br />
<br />
'''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB1A3''', '''ChR2'''::'''eYFP'''::1C3, '''pSB1T3''' from Miniprep on [[Team:Queens-Canada/22 September 2010|22/09/10]]<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Chris and Basia<br />
Digestion<br />
Chris and Basia<br />
<br />
'''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB1A3''', '''ChR2'''::'''eYFP'''::1C3, '''pSB1T3''' from Digestion on [[Team:Queens-Canada/29 September 2010|29/09/10]]<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/23_September_2010
Team:Queens-Canada/23 September 2010
2010-10-27T06:16:47Z
<p>ThaiPhi: New page: <h2>September 23, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Thai Heat Shock products from [[Team:Queens-Canada/21 Sept...</p>
<hr />
<div><br />
<h2>September 23, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Thai<br />
Heat Shock products from [[Team:Queens-Canada/21 September 2010|21/09/10]]<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/22_September_2010
Team:Queens-Canada/22 September 2010
2010-10-27T06:16:29Z
<p>ThaiPhi: New page: <h2>September 22, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Chris and Mike '''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB1A...</p>
<hr />
<div><br />
<h2>September 22, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Chris and Mike<br />
<br />
'''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB1A3''', '''ChR2'''::'''eYFP'''::1C3, '''pSB1T3''' from Liquid Culture on [[Team:Queens-Canada/21 September 2010|21/09/10]]<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/21_September_2010
Team:Queens-Canada/21 September 2010
2010-10-27T06:16:12Z
<p>ThaiPhi: New page: <h2>September 21, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Steve '''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pS...</p>
<hr />
<div><br />
<h2>September 21, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Steve<br />
<br />
'''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB1A3''', '''ChR2'''::'''eYFP'''::1C3, '''pSB1T3'''<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Yuli and Basia<br />
<br />
'''pSip-1''', '''pGpd-2''', '''pMec-7''', '''pHsp-3'''::'''mCherry'''::'''unc-54 3'UTR''', '''eYFP'''::'''unc-54 3'UTR'''<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/17_September_2010
Team:Queens-Canada/17 September 2010
2010-10-27T06:15:52Z
<p>ThaiPhi: New page: <h2>September 17, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Ligation</h3> Yuli and Mary '''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB1A3...</p>
<hr />
<div><br />
<h2>September 17, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Yuli and Mary<br />
<br />
'''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB1A3''', '''ChR2'''::'''eYFP'''::1C3, '''pSB1T3'''<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Nelson and Chris<br />
<br />
'''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB1A3''', '''ChR2'''::'''eYFP'''::1C3, '''pSB1T3'''<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/14_September_2010
Team:Queens-Canada/14 September 2010
2010-10-27T06:15:30Z
<p>ThaiPhi: New page: <h2>September 14, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion</h3> Steve and Yuli '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3 Cut...</p>
<hr />
<div><br />
<h2>September 14, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Steve and Yuli<br />
<br />
'''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
Cut with EcoRI and PstI.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Steve and Nelson<br />
<br />
'''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3, '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3 Digest<br />
<br />
Used 0.5 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and Nelson and Hao<br />
<br />
'''NpHR'''::'''eCFP'''::1C3, '''eGFP'''::1C3, '''pSB1A3''', '''ChR2'''::'''eYFP'''::1C3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/2_September_2010
Team:Queens-Canada/2 September 2010
2010-10-27T06:15:09Z
<p>ThaiPhi: New page: <h2>September 2, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Steve and Chris '''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR...</p>
<hr />
<div><br />
<h2>September 2, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Steve and Chris<br />
<br />
'''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Steve and Chris<br />
<br />
'''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Chris and Steve<br />
<br />
'''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR'''::1A3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/31_August_2010
Team:Queens-Canada/31 August 2010
2010-10-27T06:14:44Z
<p>ThaiPhi: </p>
<hr />
<div><h2>August 31, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Hao and Thai<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Hao and Thai<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Thai<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Hao and Chris<br />
'''PRF4''', '''eYFP''', '''eGFP'''<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Chris<br />
PRF4<br />
<br />
Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Hao and Chris<br />
<br />
'''eGFP'''::'''unc-54 3'UTR'''::1A3, '''eYFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1A3<br />
<br />
{| border = "1"<br />
| EcoRI and SpeI<br />
| XbaI and PstI<br />
| EcoRI and PstI<br />
|-<br />
| '''eYFP'''<br />
| '''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''eGFP'''<br />
| '''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''mCherry'''<br />
| '''unc-54 3'UTR'''<br />
| 1A3<br />
|}<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao and Chris<br />
<br />
'''eGFP'''::'''unc-54 3'UTR'''::1A3, '''eYFP'''::'''unc-54 3'UTR'''::1A3, '''mCherry'''::'''unc-54 3'UTR'''::1A3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/31_August_2010
Team:Queens-Canada/31 August 2010
2010-10-27T06:14:15Z
<p>ThaiPhi: New page: <h2>August 31, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Hao and Thai '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-...</p>
<hr />
<div><h2>August 31, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Hao and Thai<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Hao and Thai<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Thai<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Hao and Chris<br />
'''PRF4''', '''eYFP''', '''eGFP'''<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Chris<br />
PRF4<br />
<br />
Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Hao and Chris<br />
<br />
'''eGFP'''::'''unc-54 3'UTR'''::1A3, '''eYFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1A3<br />
<br />
{|<br />
| EcoRI and SpeI<br />
| XbaI and PstI<br />
| EcoRI and PstI<br />
|-<br />
| '''eYFP'''<br />
| '''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''eGFP'''<br />
| '''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''mCherry'''<br />
| '''unc-54 3'UTR'''<br />
| 1A3<br />
|}<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao and Chris<br />
<br />
'''eGFP'''::'''unc-54 3'UTR'''::1A3, '''eYFP'''::'''unc-54 3'UTR'''::1A3, '''mCherry'''::'''unc-54 3'UTR'''::1A3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/30_August_2010
Team:Queens-Canada/30 August 2010
2010-10-27T06:13:17Z
<p>ThaiPhi: New page: <h2>August 30, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Thai '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''...</p>
<hr />
<div><br />
<h2>August 30, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Thai<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Hao and Thai<br />
<br />
'''pSip-1'''::1C3, '''pFlp-1'''::1C3, '''pOdr-10'''::1C3, '''mCherry'''::1C3<br />
<br />
{|<br />
| EcoRI and SpeI<br />
| XbaI and PstI<br />
| EcoRI and PstI<br />
|-<br />
| '''pStr-220'''::1C3<br />
| '''eCFP'''::'''unc-54 3'UTR'''::1T3<br />
| '''pSB1T3'''<br />
|-<br />
|mCherry::1C3<br />
| '''unc-54 3'UTR'''::1C3<br />
| <br />
|-<br />
| '''pFlp-1'''::1C3<br />
| <br />
| <br />
|-<br />
| '''pSip-1'''::1C3<br />
| <br />
| <br />
|-<br />
| '''pOdr-10'''::1C3<br />
| <br />
| <br />
|}<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Hao and Thai<br />
<br />
'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3<br />
<br />
{|<br />
| EcoRI and SpeI<br />
| XbaI and PstI<br />
| EcoRI and PstI<br />
|-<br />
| '''pStr-1'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''pStr-220'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''pFlp-1'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3 <br />
|-<br />
| '''pOdr-10'''<br />
| '''eCFP'''::'''unc-54 3'UTR'''<br />
| 1A3<br />
|-<br />
| '''mCherry'''<br />
| '''unc-54 3'UTR'''<br />
| 1T3<br />
|}<br />
<br />
Backbone treated with CIAP for 30 mins.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao<br />
<br />
'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3<br />
<br />
Transformed ligation products from [[Team:Queens-Canada/30 August 2010|30/08/10]]<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/27_August_2010
Team:Queens-Canada/27 August 2010
2010-10-27T06:12:40Z
<p>ThaiPhi: New page: <h2>August 27, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Gel Electrophoresis</h3> Yuli and Hao '''pSip-1'''::1C3 <h3>[[Team:Queens-...</p>
<hr />
<div><br />
<h2>August 27, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Hao<br />
<br />
'''pSip-1'''::1C3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Yuli and Chris<br />
<br />
'''pSra-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/26_August_2010
Team:Queens-Canada/26 August 2010
2010-10-27T06:12:14Z
<p>ThaiPhi: New page: <h2>August 26, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Yuli and Hao '''pStr-1''', '''pMec-7''', '''pOdr-1''', '''pOsm-10''', ''...</p>
<hr />
<div><br />
<h2>August 26, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Yuli and Hao<br />
<br />
'''pStr-1''', '''pMec-7''', '''pOdr-1''', '''pOsm-10''', '''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and Hao<br />
<br />
'''pStr-1''', '''pMec-7''', '''pOdr-1''', '''pOsm-10''', '''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
Cut with EcoRI and PstI<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Hao<br />
<br />
'''pStr-1''', '''pMec-7''', '''pOdr-1''', '''pOsm-10''', '''eCFP'''::'''unc-54 3'UTR'''::1A3<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Yuli and Hao<br />
<br />
'''pGpd-2''', '''pHsp-3''', '''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pMec-7'''::'''eCFP''':'''unc-54 3'UTR'''::1C3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and Hao<br />
<br />
'''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR''', '''pGpd-2'''::'''pHsp-3'''::'''unc-54 3'UTR''', '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3. C::3::'''unc-54 3'UTR'''::1C3, '''pSB1A3'''<br />
<br />
Cut with EcoRI and PstI.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Chris<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/25_August_2010
Team:Queens-Canada/25 August 2010
2010-10-27T06:11:54Z
<p>ThaiPhi: New page: <h2>August 25, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Chris and Yuli '''pFlp-1''', '''pSip-1''', '''mCherry''', '''pOdr-10''',...</p>
<hr />
<div><br />
<h2>August 25, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Chris and Yuli<br />
<br />
'''pFlp-1''', '''pSip-1''', '''mCherry''', '''pOdr-10''', '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR''', '''pRab-7''', '''pCeh-12'''<br />
<br />
Note: [1] '''pFlp-1'''::1C3 was dropped in the waste beaker (open) before solution I was added - probably contaminated.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Chris and Yuli<br />
<br />
'''pFlp-1''', '''pSip-1''', '''mCherry''', '''pOdr-10''', '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR''', '''pRab-7''', '''pCeh-12'''<br />
<br />
Split into one 40-lane gel and one 28-lane gel.<br />
Used 1 g agarose in 140 mL TBE. Gels run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli<br />
<br />
'''pFlp-1'''::1C3, '''pSip-1'''::1C3, '''mCherry'''::1C3, '''pOdr-10'''::1C3, '''ChR2'''::'''eYFP'''::1C3 N, '''pRab-7'''::1C3, '''pCeh-12'''::1C3<br />
<br />
Cut with NotI.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Yuli<br />
<br />
'''pStr-1''', '''pGpd-2''', '''pHsp-3''', '''pSra-10''', '''pOdr-1''', '''pOsm-10''', '''pMec-7''', '''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pMec-7'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/24_August_2010
Team:Queens-Canada/24 August 2010
2010-10-27T06:11:36Z
<p>ThaiPhi: New page: <h2>August 24, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Glycerol Stock</h3> Yuli '''NpHR'''::'''eCFP''', '''unc-54 3'UTR''', '''pGpd-2''...</p>
<hr />
<div><br />
<h2>August 24, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]</h3><br />
Yuli<br />
<br />
'''NpHR'''::'''eCFP''', '''unc-54 3'UTR''', '''pGpd-2''', '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1T3, '''eCFP'''::'''unc-54 3'UTR'''::1T3<br />
<br />
Composition: 270µL 80% glycerol, 480 µL ddH2O, 750 µL cell culture.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Yuli<br />
<br />
'''pMec-7'''::1A3, '''pGpd-2'''::1C3, '''pHsp-3'''::1C3, '''pSra-10'''::1C3, '''pOdr-1'''::1C3, '''pOsm-10'''::1C3, '''pStr-1'''::1C3, '''eCFP'''::'''unc-54 3'UTR'''::1T3, '''pSB1A3''', '''pSB1C3'''<br />
<br />
Used T4 ligase. Products transformed into TOP10.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Sequencing Prep|Sequencing Prep]]</h3><br />
Chris<br />
<br />
'''eYFP''', '''eCFP''', '''NpHR''', '''pMec-7''', '''pStr-1''', '''pOsm-10''', '''unc-54 3'UTR''', '''pSra-10''', '''eGFP''', '''ChR2'''::'''eYFP'''::1C3, '''pHsp-3''', '''pGpd-2''', '''pOdr-1''', '''NpHR'''::'''eCFP''', '''pStr-220''', '''eCFP'''::'''unc-54 3'UTR'''<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Chris<br />
<br />
'''pCeh-12'''::1C3, '''pRab-7'''::1C3, '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3, '''pOdr-10'''::1C3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/23_August_2010
Team:Queens-Canada/23 August 2010
2010-10-27T06:11:11Z
<p>ThaiPhi: New page: <h2>August 23, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion</h3> Yuli '''pSra-10'''::1C3, '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1...</p>
<hr />
<div><br />
<h2>August 23, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli<br />
<br />
'''pSra-10'''::1C3, '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1C3<br />
<br />
Cut with NotI.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli<br />
<br />
'''pSip-1''', '''pRab-7''', '''mCherry''', '''pFlp-1''', '''pOdr-10''', '''pSra-10'''::1C3 (NotI digest), '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1T3 (NotI digest)<br />
<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Ligation|Ligation]]</h3><br />
Yuli<br />
<br />
'''pSip-1''', '''pRab-7''', '''mCherry''', '''pFlp-1''', '''pOdr-10''', '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR''', '''pSB1C3'''<br />
<br />
Backbone treated with CIAP for 30 mins. Used T4 ligase.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Yuli<br />
Ligation products from [[Team:Queens-Canada/23 August 2010|23/08/10]]<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
<br />
'''pMec-7''', '''pGpd-2''', '''pHsp-3''', '''pSra-10''', '''pOdr-1''', '''pOsm-10''', '''pStr-1''', '''eCFP'''::'''unc-54 3'UTR'''::1T3, '''pSB1A3'''<br />
<br />
<br />
'''pStr-1'''::1C3, '''pMec-7'''::1A3, '''pOdr-1'''::1C3, '''pOsm-10'''::1C3, '''pHsp-3'''::1C3, '''pSra-10'''::1C3 cut wih EcoRI and SpeI.<br />
<br />
'''eCFP'''::'''unc-54 3'UTR'''::1T3 cut with XbaI and PstI. Backbone treated with CIAP.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/20_August_2010
Team:Queens-Canada/20 August 2010
2010-10-27T06:10:41Z
<p>ThaiPhi: New page: <h2>August 20, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Yuli and Thai '''NpHR'''::'''eCFP'''::1C3, '''unc-54 3'UTR'''::1C3, '''p...</p>
<hr />
<div><br />
<h2>August 20, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3><br />
Yuli and Thai<br />
<br />
'''NpHR'''::'''eCFP'''::1C3, '''unc-54 3'UTR'''::1C3, '''pGpd-2'''::1C3, '''pCeh-12'''::1C3, '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1T3, 3::'''unc-54 3'UTR'''::1T3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Chris and Yuli<br />
<br />
'''NpHR'''::'''eCFP'''::1C3, '''unc-54 3'UTR'''::1C3, '''pGpd-2'''::1C3, '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1T3, '''pCeh-12'''::1C3, '''eCFP'''::'''unc-54 3'UTR'''::1T3<br />
<br />
Split into one 28-lane and one 40-lane gel.<br />
Used 1.4 g agarose in 140 mL TBE. Gels run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]</h3><br />
Chris and Yuli<br />
<br />
'''eYFP''', '''pHsp-3''', '''pMec-7''', '''pStr-220''', '''pSra-10'''<br />
<br />
Compostion: 270 µL 80% Glycerol, 480 µL ddH2O, 750 µL cell culture.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/19_August_2010
Team:Queens-Canada/19 August 2010
2010-10-27T06:10:18Z
<p>ThaiPhi: New page: <h2>August 19, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Minprep</h3> Yuli and Thai '''pHsp-3'''::1C3, '''pStr-220'''::1C3, '''pMec-7'''::1C3, '...</p>
<hr />
<div><br />
<h2>August 19, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#Minprep|Minprep]]</h3><br />
Yuli and Thai<br />
<br />
'''pHsp-3'''::1C3, '''pStr-220'''::1C3, '''pMec-7'''::1C3, '''eYFP'''::1C3, '''pSra-10'''::1C3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and Chris<br />
<br />
'''pHsp-3''', '''pStr-220''', '''pMec-7''', '''eYFP''', '''pSra-10'''<br />
<br />
Cut with EcoRI and PstI.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Yuli and Chris<br />
<br />
'''pHsp-3''', '''pStr-220''', '''pMec-7''', '''eYFP''', '''pSrb-6'''<br />
<br />
Split into one 40-lane and one 28-lane gel.<br />
Used 1.4 g agarose in 140 mL TBE. Gels run for 40 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Chris and Yuli and Thai<br />
<br />
<br />
'''NpHR'''::'''eCFP'''::1C3<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi
http://2010.igem.org/Team:Queens-Canada/18_August_2010
Team:Queens-Canada/18 August 2010
2010-10-27T06:09:28Z
<p>ThaiPhi: New page: <h2>August 18, 2010</h2> {{:Team:Queens-Canada/head}} <h3>PCR</h3> Yuli '''pGpd-2''' Program run using 9:1 FAST:HIFI blend with an extension time of...</p>
<hr />
<div><br />
<h2>August 18, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Yuli<br />
<br />
'''pGpd-2'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 3 s and annealing temperature of 57 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3><br />
Yuli and Hao<br />
<br />
<br />
'''unc-54 3'UTR''', '''pCeh-12''', 7::'''eCFP''', '''pSip-1'''::'''eGFP''', '''pGpd-2''', '''ChR2'''::'''eYFP''', 3<br />
<br />
<br />
'''unc-54 3'UTR''', '''pGpd-2''', and 1C3 cut with XbaI and PstI. All others cut with EcoRI and PstI. <br />
<br />
<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3><br />
Hao<br />
<br />
'''unc-54 3'UTR'''::1C3, '''pCeh-12'''::1C3, '''NpHR'''::'''eCFP'''::1C3, '''pGpd-2'''::1C3, '''pSip-1'''::'''eGFP'''::1T3, '''pSB1C3''', '''pSB1T3''', '''eCFP'''::'''unc-54 3'UTR'''::1T3, '''ChR2'''::'''eYFP'''::'''unc-54 3'UTR'''::1T3<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3><br />
Yuli and Hao<br />
<br />
'''eYFP''', '''pSra-10''', '''pMec-7''', '''pHsp-3'''<br />
<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Thai<br />
<br />
'''pSrb-6'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 48 s and annealing temperature of 59 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Thai<br />
<br />
'''pGpd-2'''<br />
<br />
Used 0.3 g agarose in 45 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>
ThaiPhi