http://2010.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=500&target=McCoCo&year=&month=2010.igem.org - User contributions [en]2024-03-29T01:44:21ZFrom 2010.igem.orgMediaWiki 1.16.5http://2010.igem.org/Team:MIT_safetyTeam:MIT safety2010-10-28T02:33:13Z<p>McCoCo: </p>
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<div class="bodybaby">safety</div><br><br />
<h4>Would any of your project ideas raise safety issues in terms of:</h4><br />
<h5><ul><br />
<li>researcher safety</li><br />
<li>public safety, or</li><br />
<li>environmental safety?</li><br />
</ul></h5><br />
<hr><br />
<p>This summer, our team branched off into the bacterial team and the mammalian team. The bacterial team worked in a BSL1 lab setting, and the mammalian team worked in both a BSL1 and a BSL2 lab. Both teams adhered to <a href="http://www.who.int/csr/resources/publications/biosafety/en/Biosafety7.pdf">national</a> and <a href="http://ehs.mit.edu/site/content/biosafety-resources-and-links">local</a> safety protocols. <br />
Extra care was taken to not cross-contaminate lab spaces. A limited number of students worked with bacteriophage and mammalian cells. Work with phage and all materials involved with phage were confined to one fume hood, and the mammalian team worked in a BSL2 lab, separate from the bacterial team. Cross contamination from these settings were minimized by designating equipment specifically for phage, mammalian cells, and bacteria, as well as immediate change of personal protective equipment in moving between the different lab spaces.<br />
<br> <br />
<br><br />
<h4>Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? </h4><br />
<hr><br />
<p>Neither our bacteria nor our mammalian cells contain BioBrick parts that code for hazardous proteins or molecules.</p><br />
<br><br />
<br><br />
<h4>Is there a local biosafety group, committee, or review board at your institution? </h4><br />
<hr><br />
<p>The <a href="http://ehs.mit.edu/site/">EHS (Environment, Health, and Safety) Office</a> is MIT's biosafety group that enforces lab safety in all labs on campus. They provide safety training, waste management services, and resources for safe lab practices. All undergraduates were trained by the EHS to work safely in BSL1 labs, and students working with mammalian cells underwent BSL2 lab safety training. Throughout the summer, the EHS helped us safely manage our biohazard waste. </p><br />
<br><br />
<br><br />
<h4>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </h4><br />
<hr><br />
Our <a href="https://static.igem.org/mediawiki/2010/3/3e/Mammoblock_RFC_Draft.pdf">MammoBlock construction standard</a> not only aids in the construction of mammalian parts but also facilitates safer and easier storage of these parts. The MammoBlock standard uses bacterial entry vectors which allows mammalian parts to be stored in BSL1 conditions. This standard has allowed us to enter mammalian parts into the registry, and will facilitate the safe shipping and submission of mammalian parts constructed by future iGEM teams. <br><br />
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</html></div>McCoCohttp://2010.igem.org/Team:MIT_safetyTeam:MIT safety2010-10-28T02:31:49Z<p>McCoCo: </p>
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<div class="bodybaby">safety</div><br><br />
<h4>Would any of your project ideas raise safety issues in terms of:</h4><br />
<h5><ul><br />
<li>researcher safety</li><br />
<li>public safety, or</li><br />
<li>environmental safety?</li><br />
</ul></h5><br />
<hr><br />
<p>This summer, our team branched off into the bacterial team and the mammalian team. The bacterial team worked in a BSL1 lab setting, and the mammalian team worked in both a BSL1 and a BSL2 lab. Both teams adhered to <a href="http://www.who.int/csr/resources/publications/biosafety/en/Biosafety7.pdf">national</a> and <a href="http://ehs.mit.edu/site/content/biosafety-resources-and-links">local</a> safety protocols. <br />
Extra care was taken to not cross-contaminate lab spaces. A limited number of students worked with bacteriophage and mammalian cells. Work with phage and all materials involved with phage were confined to one fume hood, and the mammalian team worked in a BSL2 lab, separate from the bacterial team. Cross contamination from these settings were minimized by designating equipment specifically for phage, mammalian cells, and bacteria, as well as immediate change of personal protective equipment in moving between the different lab spaces.<br />
<br> <br />
<br><br />
<h4>Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? </h4><br />
<hr><br />
<p>Neither our bacteria nor our mammalian cells contain BioBrick parts that code for hazardous proteins or molecules.</p><br />
<br><br />
<br><br />
<h4>Is there a local biosafety group, committee, or review board at your institution? </h4><br />
<hr><br />
<p>The <a href="http://ehs.mit.edu/site/">EHS (Environment, Health, and Safety) Office</a> is MIT's biosafety group that enforces lab safety in all labs on campus. They provide safety training, waste management services, and resources for safe lab practices. All undergraduates were trained by the EHS to work safely in BSL1 labs, and students working with mammalian cells underwent BSL2 lab safety training. Throughout the summer, the EHS helped us safely manage our biohazard waste. </p><br />
<br><br />
<br><br />
<h4>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </h4><br />
<hr><br />
Our MammoBlock construction standard not only aids in the construction of mammalian parts but also facilitates safer and easier storage of these parts. The MammoBlock standard uses bacterial entry vectors which allows mammalian parts to be stored in BSL1 conditions. This standard has allowed us to enter mammalian parts into the registry, and will facilitate the safe shipping and submission of mammalian parts constructed by future iGEM teams. <br><br />
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</html></div>McCoCohttp://2010.igem.org/Team:MIT_phage_resultsTeam:MIT phage results2010-10-28T01:52:24Z<p>McCoCo: </p>
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<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tmodel">Modelling</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_composite">Characterization</a></li><br />
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</dd><br />
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<dt><b>Phage</b></dt><br />
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<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
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</ul><br />
</dd><br />
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<dt><b>Mammalian</b></dt><br />
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<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
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</ul><br />
</dd><br />
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<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">hairy cells and polymerizing phage - results</div></td><br />
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<b>Experimental Aims</b><br />
<br><br />
This summer, we pursued three main experimental aims: <br> <br />
<ol><br />
<li> to create a system for inducible polyphage formation </li><br />
<li> to display fusion proteins on the phage coat</li><br />
<li> to demonstrate cross-linking </li><br />
</ol><br />
<br><br />
<b> Polyphage formation </b><br />
<br><br />
Rakonjac and Model (1998) showed that in the absence of pIII, elongation of the phage proceeds without termination, and the assembling phage stay associated with the cells. We were able to reproduce this phenotype with cells transformed with the hyperphage phagemid.<br />
<br><br />
<br><br />
Cells that were transformed with the hyperphage phagemid show a dense mat of many long filaments extending from the cell.<br />
<br><br />
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<div>Figure 1 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" width="300px" border=0><br />
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<br/><br />
<u>Figure 1. AFM Image of Hyperphage-Transformed Cell</u></br><br />
DH5a-pro cells were transformed with hyperphage, then grown with low shaking for 10 hours, to prevent shearing of the phage. The polyphage are the long rod structures that fan out around the cell. The round artifacts immediately surrounding the cell is likely disrupted membrane, resulting from washing the cells in water instead of a salt buffered solution.<br />
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In contrast, when we transformed with hyperphage and our pIII producing plasmid, BBa_K415138, we observed that the polyphage were absent. <br />
<br><br />
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<div>Figure 2 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" width="300px" border=0><br />
</a><br />
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<p style="padding:10px"><br />
<br/><br />
<u>Figure 2. AFM Image of Hyperphage/BBa_K415138 Co-Transformed Cells</u></br><br />
DH5a-pro cells were transformed with hyperphage and our p3 generator, BBa_K415138, then grown with low shaking for 10 hours. No polyphage structures are apparent. Presumably, these cells are producing single phage.<br />
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<div>Figure 3 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" width="300px" border=0><br />
</a><br />
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<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 3. AFM Image of M13K07 transformed cells</u></br><br />
DH5a-pro cells were transformed with M13K07 then grown with low shaking for 10 hours. Note the similarity to <u>Figure 2</u>: No polyphage structures are apparent. These cells produce single phage.<br />
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<br><br />
<b> Displaying Fusion Proteins </b><br />
<br><br />
In testing for the incorporation of our fusion into the phage coat, we chose to have M13K07 package our fusion instead of hyperphage. Testing with hyperphage would be difficult because the 100-fold reduction in infectivity (Rakonjac and Model, 1998) and the property of being tethered to the membrane introduce complications in the necessary amplification and purification steps. M13K07 and Hyperphage are equivalent in all other aspects, so M13K07 makes an ideal system for testing the ability of our fusions to be incorporated into the phage coat. We transformed cells with our fusions, infected them with the M13K07 helper phage, and purified the resulting amplified phage, then western blotted with HA and Myc antibodies. We did not induce cultures because our fusions were under control of R0065, a very leaky promoter, and we postulated that the basal level of expression would be enough for incorporation.<br />
<br><br><br />
In the western blot, we observed signals from p8-Fos* and p8-GR1. The other linkers may require some tuning of induction or additional troubleshooting before we observe incorporation.<br />
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<td width="33%"><br />
<div>Figure 4 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" width="300px" border=0><br />
</a><br />
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<p style="padding:10px"><br />
<br/><br />
<u>Figure 4. Western Blot of p8-Linkers</u></br><br />
Of our fusions, p8-Fos, p8-Base, and p8-GR2 are tagged with Myc, and p8-Jun, p8-Acid and p8-GR1 are tagged with HA. Purified M13K07 and M13KE were run as negative controls (neither have fusion protiens). p8-GR1 was run on the Myc blot as an additional negative control, as p8-GR1 lacks the Myc tag. In all cases, about 10^11 phage were loaded onto the gel.<br />
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<font size = "1.5"><br />
*Side note: Interestingly, p8-Fos shows a band at the expected 13 kD and another band at 15kD. One speculation is that because we changed an Alanine to a Leucine at the cleavage site (the joining between the p8 leader sequence and the coil), we may have created a fusion that is cleaved at a lower efficiency. This speculation requires additional thought and research, however; it will be considered in the debugging of the other p8-linkers.<br />
</font><br />
<br><br />
<br><br />
<br />
<b> Cross-linking </b><br><br />
Over the course of the summer, we've found that taking AFM images is difficult and time-consuming. Our original plan was to take AFM images of cross-linking hyperphage. After repeated attempts and little success, we realized that we needed to have a fast way of debugging our system. <br />
<br><br />
To facilitate fast debugging, we designed a system that would couple the heterodimerization of GR1 and GR2 to a fluorescent output, via split GFP.<br />
<br><br />
<a href="https://static.igem.org/mediawiki/2010/a/aa/SplitGFP.gif"><img src="https://static.igem.org/mediawiki/2010/3/3e/SplitGFPdeux.gif" width=60%></a><br />
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<div style="text-align:center"><br />
&larr; <a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a><br />
&nbsp;<br />
&nbsp;<br />
&nbsp;<br />
<a href="https://2010.igem.org/Team:MIT_phage_context">Context</a> &rarr;<br />
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</html></div>McCoCohttp://2010.igem.org/File:SplitGFPdeux.gifFile:SplitGFPdeux.gif2010-10-28T01:51:06Z<p>McCoCo: </p>
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<div></div>McCoCohttp://2010.igem.org/Team:MIT_mammalian_StandardTeam:MIT mammalian Standard2010-10-28T00:19:37Z<p>McCoCo: </p>
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<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tmodel">Modelling</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tchara">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
</dl><br />
<dl id="specialnav"><br />
<dt><b>Mammalian</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
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<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">New Mammalian Assembly Standard </div></td><br />
<tr><td><br />
<br />
Mammalian promoters are difficult to biobrick. They're several kB in length, much longer then their prokaryotic counterparts. This means they're likely to contain most restriction sites used in biobrick cloning. It's difficult to avoid this by changing the promoter sequence; single base pair mutations often alter or abolish the desired function of the promoter. To get around this issue, we've created a new standardization for cloning in mammalian cells, based on the Invitrogen Gateway (c) cloning system. <A HREF="https://static.igem.org/mediawiki/2010/3/3e/Mammoblock_RFC_Draft.pdf">Read our full 'MammoBlock' standardization proposal</A> <br />
<br><br><br />
<table><tr><td><br />
<a href="https://static.igem.org/mediawiki/2010/e/ed/Gateway_image.tiff" class="thickbox" title="Multi-site LR Gateway Reaction"><img src="https://static.igem.org/mediawiki/2010/e/ed/Gateway_image.tiff" width=300px></a></td><td><br />
Gateway (c) is a fast and reliable way to create expression vectors for mammalian cells. It fulfills the same function as restriction cloning in the Biobrick standardization - it allows us to combine vectors with different 'parts' to create a whole 'circuit'. But the actual mechanism is vastly different from restriction cloning. Gateway uses recombination enzymes to combine multiple vectors, a one-step process that avoids the laborious digestion and ligation steps involved in restriction cloning.</td></tr></table><br />
<br><br />
<b>Cloning Process Overview</b><br />
<br><br />
We start out by cloning all the genes and promoters needed into pENTR vectors; the pENTR vectors contain restriction and recombination sites, so either cloning method can be used to insert the target DNA into the vectors. The next step is to combine pENTR vectors containing the relevant gene and promoter with a pDEST vector containing a lentiviral origin of replication. Gateway cloning allows us to avoid laborious digestion and ligation steps in favor of a faster, more efficient method. To obtain the expression vector, we combine all three plasmids in a recombination reaction; the step takes 12-16 hours total and yields remarkably reliable products.<br />
<br><br><br />
<b>Our Contribution</b><br />
<br><br />
We are in the process of adding two new backbones to the registry; pENTRL4R1 is a backbone for promoter parts (see map below). The location of the attL4 and attR1 recombination sites place the promoter in front of the gene of interest during Gateway recombination. This construct contains a TRE-inducible promoter between the recombination sites. <br />
<br><br />
<center><br />
<a href="https://static.igem.org/mediawiki/2010/0/0b/L4_TRE_R1_image.jpg" class="thickbox" title="MammoBlock Promoter Backbone"><img src="https://static.igem.org/mediawiki/2010/0/0b/L4_TRE_R1_image.jpg" width=400px></a></center><br />
<br><br />
The second backbone designed to support gene 'parts'. It contains attL1 and attL2 recombination sites flanking the gene of interest. <br />
<br><br />
<center><br />
<a href="https://static.igem.org/mediawiki/2010/3/33/L1_EGFP_L2_image.jpg" class="thickbox" title="MammoBlock Gene Backbone"><img src="https://static.igem.org/mediawiki/2010/3/33/L1_EGFP_L2_image.jpg" width=400px></a></center><br />
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<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
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<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
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<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">hairy cells and polymerizing phage - results</div></td><br />
<tr><td><br />
<br><br />
<b>Experimental Aims</b><br />
<br><br />
This summer, we pursued three main experimental aims: <br> <br />
<ol><br />
<li> to create a system for inducible polyphage formation </li><br />
<li> to display fusion proteins on the phage coat</li><br />
<li> to demonstrate cross-linking </li><br />
</ol><br />
<br><br />
<b> Polyphage formation </b><br />
<br><br />
Rakonjac and Model (1998) showed that in the absence of pIII, elongation of the phage proceeds without termination, and the assembling phage stay associated with the cells. We were able to reproduce this phenotype with cells transformed with the hyperphage phagemid.<br />
<br><br />
<br><br />
Cells that were transformed with the hyperphage phagemid show a dense mat of many long filaments extending from the cell.<br />
<br><br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 1 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 1. AFM Image of Hyperphage-Transformed Cell</u></br><br />
DH5a-pro cells were transformed with hyperphage, then grown with low shaking for 10 hours, to prevent shearing of the phage. The polyphage are the long rod structures that fan out around the cell. The round artifacts immediately surrounding the cell is likely disrupted membrane, resulting from washing the cells in water instead of a salt buffered solution.<br />
</td><br />
</tr><br />
</table><br />
<br><br />
In contrast, when we transformed with hyperphage and our pIII producing plasmid, BBa_K415138, we observed that the polyphage were absent. <br />
<br><br />
<br><br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 2 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 2. AFM Image of Hyperphage/BBa_K415138 Co-Transformed Cells</u></br><br />
DH5a-pro cells were transformed with hyperphage and our p3 generator, BBa_K415138, then grown with low shaking for 10 hours. No polyphage structures are apparent. Presumably, these cells are producing single phage.<br />
</td><br />
</tr><br />
</table><br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 3 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 3. AFM Image of M13K07 transformed cells</u></br><br />
DH5a-pro cells were transformed with M13K07 then grown with low shaking for 10 hours. Note the similarity to <u>Figure 2</u>: No polyphage structures are apparent. These cells produce single phage.<br />
</td><br />
</tr><br />
</table><br />
<br />
<br><br />
<b> Displaying Fusion Proteins </b><br />
<br><br />
In testing for the incorporation of our fusion into the phage coat, we chose to have M13K07 package our fusion instead of hyperphage. Testing with hyperphage would be difficult because the 100-fold reduction in infectivity (Rakonjac and Model, 1998) and the property of being tethered to the membrane introduce complications in the necessary amplification and purification steps. M13K07 and Hyperphage are equivalent in all other aspects, so M13K07 makes an ideal system for testing the ability of our fusions to be incorporated into the phage coat. We transformed cells with our fusions, infected them with the M13K07 helper phage, and purified the resulting amplified phage, then western blotted with HA and Myc antibodies. We did not induce cultures because our fusions were under control of R0065, a very leaky promoter, and we postulated that the basal level of expression would be enough for incorporation.<br />
<br><br><br />
In the western blot, we observed signals from p8-Fos* and p8-GR1. The other linkers may require some tuning of induction or additional troubleshooting before we observe incorporation.<br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 4 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 4. Western Blot of p8-Linkers</u></br><br />
Of our fusions, p8-Fos, p8-Base, and p8-GR2 are tagged with Myc, and p8-Jun, p8-Acid and p8-GR1 are tagged with HA. Purified M13K07 and M13KE were run as negative controls (neither have fusion protiens). p8-GR1 was run on the Myc blot as an additional negative control, as p8-GR1 lacks the Myc tag. In all cases, about 10^11 phage were loaded onto the gel.<br />
</td><br />
</tr><br />
</table><br />
<br />
<font size = "1.5"><br />
*Side note: Interestingly, p8-Fos shows a band at the expected 13 kD and another band at 15kD. One speculation is that because we changed an Alanine to a Leucine at the cleavage site (the joining between the p8 leader sequence and the coil), we may have created a fusion that is cleaved at a lower efficiency. This speculation requires additional thought and research, however; it will be considered in the debugging of the other p8-linkers.<br />
</font><br />
<br><br />
<br><br />
<br />
<b> Cross-linking </b><br><br />
Over the course of the summer, we've found that taking AFM images is difficult and time-consuming. Our original plan was to take AFM images of cross-linking hyperphage. After repeated attempts and little success, we realized that we needed to have a fast way of debugging our system. <br />
<br><br />
To facilitate fast debugging, we designed a system that would couple the heterodimerization of GR1 and GR2 to a fluorescent output, via split GFP.<br />
<br><br />
<a href="https://static.igem.org/mediawiki/2010/a/aa/SplitGFP.gif"><img src="https://static.igem.org/mediawiki/2010/a/aa/SplitGFP.gif" width=60%></a><br />
<br />
</div><br />
<br />
<div style="text-align:center"><br />
&larr; <a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a><br />
&nbsp;<br />
&nbsp;<br />
&nbsp;<br />
<a href="https://2010.igem.org/Team:MIT_phage_context">Context</a> &rarr;<br />
</div><br />
<br />
</td><br />
</table><br />
<br />
<br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_phage_resultsTeam:MIT phage results2010-10-27T22:31:03Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
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<head><br />
<style><br />
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<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
</dl><br />
<dl id ="specialnav"><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
</dl><br />
<dl id ="nav"><br />
<dt><b>Mammalian</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">hairy cells and polymerizing phage - results</div></td><br />
<tr><td><br />
<br><br />
<b>Experimental Aims</b><br />
<br><br />
This summer, we pursued three main experimental aims: <br> <br />
<ol><br />
<li> to create a system for inducible polyphage formation </li><br />
<li> to display fusion proteins on the phage coat</li><br />
<li> to demonstrate cross-linking </li><br />
</ol><br />
<br><br />
<b> Polyphage formation </b><br />
<br><br />
Rakonjac and Model (1998) showed that in the absence of pIII, elongation of the phage proceeds without termination, and the assembling phage stay associated with the cells. We were able to reproduce this phenotype with cells transformed with the hyperphage phagemid.<br />
<br><br />
<br><br />
Cells that were transformed with the hyperphage phagemid show a dense mat of many long filaments extending from the cell.<br />
<br><br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 1 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 1. AFM Image of Hyperphage-Transformed Cell</u></br><br />
DH5a-pro cells were transformed with hyperphage, then grown with low shaking for 10 hours, to prevent shearing of the phage. The polyphage are the long rod structures that fan out around the cell. The round artifacts immediately surrounding the cell is likely disrupted membrane, resulting from washing the cells in water instead of a salt buffered solution.<br />
</td><br />
</tr><br />
</table><br />
<br><br />
In contrast, when we transformed with hyperphage and our pIII producing plasmid, BBa_K415138, we observed that the polyphage were absent. <br />
<br><br />
<br><br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 2 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 2. AFM Image of Hyperphage/BBa_K415138 Co-Transformed Cells</u></br><br />
DH5a-pro cells were transformed with hyperphage and our p3 generator, BBa_K415138, then grown with low shaking for 10 hours. No polyphage structures are apparent. Presumably, these cells are producing single phage.<br />
</td><br />
</tr><br />
</table><br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 3 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 3. AFM Image of M13K07 transformed cells</u></br><br />
DH5a-pro cells were transformed with M13K07 then grown with low shaking for 10 hours. Note the similarity to <u>Figure 2</u>: No polyphage structures are apparent. These cells produce single phage.<br />
</td><br />
</tr><br />
</table><br />
<br />
<br><br />
<b> Displaying Fusion Proteins </b><br />
<br><br />
In testing for the incorporation of our fusion into the phage coat, we chose to have M13K07 package our fusion instead of hyperphage. Testing with hyperphage would be difficult because the 100-fold reduction in infectivity (Rakonjac and Model, 1998) and the property of being tethered to the membrane introduce complications in the necessary amplification and purification steps. M13K07 and Hyperphage are equivalent in all other aspects, so M13K07 makes an ideal system for testing the ability of our fusions to be incorporated into the phage coat. We transformed cells with our fusions, infected them with the M13K07 helper phage, and purified the resulting amplified phage, then western blotted with HA and Myc antibodies. We did not induce cultures because our fusions were under control of R0065, a very leaky promoter, and we postulated that the basal level of expression would be enough for incorporation.<br />
<br><br><br />
In the western blot, we observed signals from p8-Fos* and p8-GR1. The other linkers may require some tuning of induction or additional troubleshooting before we observe incorporation.<br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 4 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 4. Western Blot of p8-Linkers</u></br><br />
Of our fusions, p8-Fos, p8-Base, and p8-GR2 are tagged with Myc, and p8-Jun, p8-Acid and p8-GR1 are tagged with HA. Purified M13K07 and M13KE were run as negative controls (neither have fusion protiens). p8-GR1 was run on the Myc blot as an additional negative control, as p8-GR1 lacks the Myc tag. In all cases, about 10^11 phage were loaded onto the gel.<br />
</td><br />
</tr><br />
</table><br />
<br />
<font size = "1.5"><br />
*Side note: Interestingly, p8-Fos shows a band at the expected 13 kD and another band at 15kD. One speculation is that because we changed an Alanine to a Leucine at the cleavage site (the joining between the p8 leader sequence and the coil), we may have created a fusion that is cleaved at a lower efficiency. This speculation requires additional thought and research, however; it will be considered in the debugging of the other p8-linkers.<br />
</font><br />
<br><br />
<br><br />
<br />
<b> Cross-linking </b><br><br />
Over the course of the summer, we've found that taking AFM images is difficult and time-consuming. Our original plan was to take AFM images of cross-linking hyperphage, After repeated attempts and little success, we realized that we needed to have a fast way of debugging our system. <br />
<br><br />
To facilitate fast debugging, we designed a system that would couple the heterodimerization of GR1 and GR2 to a fluorescent output, via split GFP.<br />
<br><br />
<a href="https://static.igem.org/mediawiki/2010/a/aa/SplitGFP.gif"><img src="https://static.igem.org/mediawiki/2010/a/aa/SplitGFP.gif" width=60%></a><br />
<br />
</div><br />
<br />
<div style="text-align:center"><br />
&larr; <a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a><br />
&nbsp;<br />
&nbsp;<br />
&nbsp;<br />
<a href="https://2010.igem.org/Team:MIT_phage_context">Context</a> &rarr;<br />
</div><br />
<br />
</td><br />
</table><br />
<br />
<br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_phage_resultsTeam:MIT phage results2010-10-27T22:30:06Z<p>McCoCo: </p>
<hr />
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<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
</dl><br />
<dl id ="specialnav"><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
</dl><br />
<dl id ="nav"><br />
<dt><b>Mammalian</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">hairy cells and polymerizing phage - results</div></td><br />
<tr><td><br />
<br><br />
<b>Experimental Aims</b><br />
<br><br />
This summer, we pursued three main experimental aims: <br> <br />
<ol><br />
<li> to create a system for inducible polyphage formation </li><br />
<li> to display fusion proteins on the phage coat</li><br />
<li> to demonstrate cross-linking </li><br />
</ol><br />
<br><br />
<b> Polyphage formation </b><br />
<br><br />
Rakonjac and Model (1998) showed that in the absence of pIII, elongation of the phage proceeds without termination, and the assembling phage stay associated with the cells. We were able to reproduce this phenotype with cells transformed with the hyperphage phagemid.<br />
<br><br />
<br><br />
Cells that were transformed with the hyperphage phagemid show a dense mat of many long filaments extending from the cell.<br />
<br><br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 1 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 1. AFM Image of Hyperphage-Transformed Cell</u></br><br />
DH5a-pro cells were transformed with hyperphage, then grown with low shaking for 10 hours, to prevent shearing of the phage. The polyphage are the long rod structures that fan out around the cell. The round artifacts immediately surrounding the cell is likely disrupted membrane, resulting from washing the cells in water instead of a salt buffered solution.<br />
</td><br />
</tr><br />
</table><br />
<br><br />
In contrast, when we transformed with hyperphage and our pIII producing plasmid, BBa_K415138, we observed that the polyphage were absent. <br />
<br><br />
<br><br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 2 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 2. AFM Image of Hyperphage/BBa_K415138 Co-Transformed Cells</u></br><br />
DH5a-pro cells were transformed with hyperphage and our p3 generator, BBa_K415138, then grown with low shaking for 10 hours. No polyphage structures are apparent. Presumably, these cells are producing single phage.<br />
</td><br />
</tr><br />
</table><br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 3 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 3. AFM Image of M13K07 transformed cells</u></br><br />
DH5a-pro cells were transformed with M13K07 then grown with low shaking for 10 hours. Note the similarity to <u>Figure 2</u>: No polyphage structures are apparent. These cells produce single phage.<br />
</td><br />
</tr><br />
</table><br />
<br />
<br><br />
<b> Displaying Fusion Proteins </b><br />
<br><br />
In testing for the incorporation of our fusion into the phage coat, we chose to have M13K07 package our fusion instead of hyperphage. Testing with hyperphage would be difficult because the 100-fold reduction in infectivity (Rakonjac and Model, 1998) and the property of being tethered to the membrane introduce complications in the necessary amplification and purification steps. M13K07 and Hyperphage are equivalent in all other aspects, so M13K07 makes an ideal system for testing the ability of our fusions to be incorporated into the phage coat. We transformed cells with our fusions, infected them with the M13K07 helper phage, and purified the resulting amplified phage, then western blotted with HA and Myc antibodies. We did not induce cultures because our fusions were under control of R0065, a very leaky promoter, and we postulated that the basal level of expression would be enough for incorporation.<br />
<br><br><br />
In the western blot, we observed signals from p8-Fos* and p8-GR1. The other linkers may require some tuning of induction or additional troubleshooting before we observe incorporation.<br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 4 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 4. Western Blot of p8-Linkers</u></br><br />
Of our fusions, p8-Fos, p8-Base, and p8-GR2 are tagged with Myc, and p8-Jun, p8-Acid and p8-GR1 are tagged with HA. Purified M13K07 and M13KE were run as negative controls (neither have fusion protiens). p8-GR1 was run on the Myc blot as an additional negative control, as p8-GR1 lacks the Myc tag. In all cases, about 10^11 phage were loaded onto the gel.<br />
</td><br />
</tr><br />
</table><br />
<br />
<font size = "1.5"><br />
*Side note: Interestingly, p8-Fos shows a band at the expected 13 kD and another band at 15kD. One speculation is that because we changed an Alanine to a Leucine at the cleavage site (the joining between the p8 leader sequence and the coil), we may have created a fusion that is cleaved at a lower efficiency. This speculation requires additional thought and research, however; it will be considered in the debugging of the other p8-linkers.<br />
</font><br />
<br><br />
<br><br />
<br />
<b> Cross-linking </b><br><br />
Over the course of the summer, we've found that taking AFM images is difficult and time-consuming. Our original plan was to take AFM images of cross-linking hyperphage, After repeated attempts and little success, we realized that we needed to have a fast way of debugging our system. <br />
<br><br />
To facilitate fast debugging, we designed a system that would couple the heterodimerization of GR1 and GR2 to a fluorescent output, via split GFP.<br />
<br><br />
<img src="https://static.igem.org/mediawiki/2010/a/aa/SplitGFP.gif" width=60%><br />
<br />
</div><br />
<br />
<div style="text-align:center"><br />
&larr; <a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a><br />
&nbsp;<br />
&nbsp;<br />
&nbsp;<br />
<a href="https://2010.igem.org/Team:MIT_phage_context">Context</a> &rarr;<br />
</div><br />
<br />
</td><br />
</table><br />
<br />
<br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_phage_resultsTeam:MIT phage results2010-10-27T22:29:23Z<p>McCoCo: </p>
<hr />
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<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
</dl><br />
<dl id ="specialnav"><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
</dl><br />
<dl id ="nav"><br />
<dt><b>Mammalian</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">hairy cells and polymerizing phage - results</div></td><br />
<tr><td><br />
<br><br />
<b>Experimental Aims</b><br />
<br><br />
This summer, we pursued three main experimental aims: <br> <br />
<ol><br />
<li> to create a system for inducible polyphage formation </li><br />
<li> to display fusion proteins on the phage coat</li><br />
<li> to demonstrate cross-linking </li><br />
</ol><br />
<br><br />
<b> Polyphage formation </b><br />
<br><br />
Rakonjac and Model (1998) showed that in the absence of pIII, elongation of the phage proceeds without termination, and the assembling phage stay associated with the cells. We were able to reproduce this phenotype with cells transformed with the hyperphage phagemid.<br />
<br><br />
<br><br />
Cells that were transformed with the hyperphage phagemid show a dense mat of many long filaments extending from the cell.<br />
<br><br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 1 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/1/17/Phage_2.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 1. AFM Image of Hyperphage-Transformed Cell</u></br><br />
DH5a-pro cells were transformed with hyperphage, then grown with low shaking for 10 hours, to prevent shearing of the phage. The polyphage are the long rod structures that fan out around the cell. The round artifacts immediately surrounding the cell is likely disrupted membrane, resulting from washing the cells in water instead of a salt buffered solution.<br />
</td><br />
</tr><br />
</table><br />
<br><br />
In contrast, when we transformed with hyperphage and our pIII producing plasmid, BBa_K415138, we observed that the polyphage were absent. <br />
<br><br />
<br><br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 2 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/27/Phage_1.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 2. AFM Image of Hyperphage/BBa_K415138 Co-Transformed Cells</u></br><br />
DH5a-pro cells were transformed with hyperphage and our p3 generator, BBa_K415138, then grown with low shaking for 10 hours. No polyphage structures are apparent. Presumably, these cells are producing single phage.<br />
</td><br />
</tr><br />
</table><br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 3 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/e/e2/Phage_3.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 3. AFM Image of M13K07 transformed cells</u></br><br />
DH5a-pro cells were transformed with M13K07 then grown with low shaking for 10 hours. Note the similarity to <u>Figure 2</u>: No polyphage structures are apparent. These cells produce single phage.<br />
</td><br />
</tr><br />
</table><br />
<br />
<br><br />
<b> Displaying Fusion Proteins </b><br />
<br><br />
In testing for the incorporation of our fusion into the phage coat, we chose to have M13K07 package our fusion instead of hyperphage. Testing with hyperphage would be difficult because the 100-fold reduction in infectivity (Rakonjac and Model, 1998) and the property of being tethered to the membrane introduce complications in the necessary amplification and purification steps. M13K07 and Hyperphage are equivalent in all other aspects, so M13K07 makes an ideal system for testing the ability of our fusions to be incorporated into the phage coat. We transformed cells with our fusions, infected them with the M13K07 helper phage, and purified the resulting amplified phage, then western blotted with HA and Myc antibodies. We did not induce cultures because our fusions were under control of R0065, a very leaky promoter, and we postulated that the basal level of expression would be enough for incorporation.<br />
<br><br><br />
In the western blot, we observed signals from p8-Fos* and p8-GR1. The other linkers may require some tuning of induction or additional troubleshooting before we observe incorporation.<br />
<br />
<table style="padding:10px;color:#000000"><br />
<tr><br />
<td width="33%"><br />
<div>Figure 4 &nbsp; <a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/><br />
<a href="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" id="single_image"><br />
<img src="https://static.igem.org/mediawiki/igem.org/b/bf/1023western2.jpg" width="300px" border=0><br />
</a><br />
</td><br />
<td style="vertical-align:top"><br />
<p style="padding:10px"><br />
<br/><br />
<u>Figure 4. Western Blot of p8-Linkers</u></br><br />
Of our fusions, p8-Fos, p8-Base, and p8-GR2 are tagged with Myc, and p8-Jun, p8-Acid and p8-GR1 are tagged with HA. Purified M13K07 and M13KE were run as negative controls (neither have fusion protiens). p8-GR1 was run on the Myc blot as an additional negative control, as p8-GR1 lacks the Myc tag. In all cases, about 10^11 phage were loaded onto the gel.<br />
</td><br />
</tr><br />
</table><br />
<br />
<font size = "1.5"><br />
*Side note: Interestingly, p8-Fos shows a band at the expected 13 kD and another band at 15kD. One speculation is that because we changed an Alanine to a Leucine at the cleavage site (the joining between the p8 leader sequence and the coil), we may have created a fusion that is cleaved at a lower efficiency. This speculation requires additional thought and research, however; it will be considered in the debugging of the other p8-linkers.<br />
</font><br />
<br><br />
<br><br />
<br />
<b> Cross-linking </b><br><br />
Over the course of the summer, we've found that taking AFM images is difficult and time-consuming. Our original plan was to take AFM images of cross-linking hyperphage, After repeated attempts and little success, we realized that we needed to have a fast way of debugging our system. <br />
<br><br />
To facilitate fast debugging, we designed a system that would couple the heterodimerization of GR1 and GR2 to a fluorescent output, via split GFP.<br />
<br><br />
<img scr="https://static.igem.org/mediawiki/2010/a/aa/SplitGFP.gif" width=60%><br />
<br />
</div><br />
<br />
<div style="text-align:center"><br />
&larr; <a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a><br />
&nbsp;<br />
&nbsp;<br />
&nbsp;<br />
<a href="https://2010.igem.org/Team:MIT_phage_context">Context</a> &rarr;<br />
</div><br />
<br />
</td><br />
</table><br />
<br />
<br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/File:SplitGFP.gifFile:SplitGFP.gif2010-10-27T22:10:34Z<p>McCoCo: </p>
<hr />
<div></div>McCoCohttp://2010.igem.org/Team:MIT_acknowledgementsTeam:MIT acknowledgements2010-10-27T18:16:21Z<p>McCoCo: </p>
<hr />
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<title>MIT iGEM 2010 Abstract</title><br />
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<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
<dt><b>Mammalian</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">acknowledgments</div></td><br />
<tr><td><br />
<br><br />
<h4>Thank You</h4><br />
<br><br />
<strong>Donation of the Collins toggle</strong><br />
<hr><br />
<ul><br />
<li>Collins Lab at Boston University</li><br />
</ul><br />
<br><br />
<br><br />
<strong>Participation in Bioethics Video</strong><br />
<hr><br />
<ul><br />
<li>Christopher Anderson</li><br />
<li>George Church</li><br />
<li>Drew Endy</li><br />
<li>...Evans</li><br />
<li>Wendell Lim</li><br />
<li>Jay Keasling</li><br />
<br />
</ul><br />
<br><br />
<br><br />
<br />
<strong>Help and Lab Equipment</strong><br />
<hr><br />
<ul><br />
<li>Dongyan Tan from the Walz Lab</li><br />
<li>Oksana Sergeeva and the King Lab</li><br />
</ul><br />
<br><br><br />
<br />
<strong>Use of Lab Space and Materials</strong><br />
<hr><br />
<ul><br />
<li>Belcher Lab</li><br />
<li>King Lab</li><br />
<li>Knight Lab</li><br />
<li>Walz Lab</li><br />
<li>Weiss Lab</li><br />
</ul><br />
<br><br />
<br><br />
<br />
<br />
<br />
</td><br />
</table><br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_bioethicsTeam:MIT bioethics2010-10-27T18:13:27Z<p>McCoCo: </p>
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<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="specialnav"><br />
<dt><b>Contributions</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_parts">Mammalian Standard</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_original">Bioethics</a></li><br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
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<div class="bodybaby">Bioethics</div><br><br />
<center><iframe src="http://player.vimeo.com/video/16235379" width="400" height="225" frameborder="0"></iframe><p><a href="http://vimeo.com/16235379">Patents and SynBio a Video by MIT iGEM2010</a> from <a href="http://vimeo.com/user5070380">Paul Muir</a> on <a href="http://vimeo.com">Vimeo</a>.</p></center><br />
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<br><br />
<nowiki><strong>== Patents and Synthetic Biology==</strong><br />
<br><br />
<br />
The last half century has witnessed pivotal advances in the life sciences, ranging from the elucidation of DNA's structure to the mapping of the human genome. <br />
<br />
It can be argued, however, that none has had a greater impact on our standard of living or economy than the discovery of restriction enzyme technology and its product, recombinant DNA. Facilitating innovations ranging from pharmaceutical products synthesis to gene therapy, these tools of genetic engineering birthed the biotechnology industry. <br />
<br />
Yet, the industry and its life changing products would not have been possible without the input of pioneering entrepreneurs and their own key enabling technology- patents. Through the provision of a limited monopoly, patents provided incentives not only for further research by inventors but also development from early entrepreneurs to bring their products to market. <br />
<br />
<br />
Thus secured a financial foundation, pioneering entrepreneurs quickly subscribed to the motto “clone a gene, make a million,” anticipating support from biopharmaceuticals and the traditional venture capital community. Indeed, commercial biotechnology started with high hopes. "There's no question that [...] the entrepreneurs who began biotechnology businesses had very broad, grand ideas and big visions," says Jim Vincent, former chairman and CEO of Biogen, Inc. <br />
<br />
With patents securing compensation on the most promising products, money poured into biotechnology startups such as Amgen, Genzyme, and Biogen, forming a $30 billion industry with over 160 drugs and vaccines on the market and another 370 currently undergoing clinical trials. With a market capitalization of over $300 billion and 200,000 employees working for 1,500 companies in the US alone, biotechnology's rise directly follows from the increased mapping and staking of biological intellectual property space. <br />
<br />
Co-founder, president, and COO of Advanced Tissue Sciences, Inc., Gail Naughton made it a point to secure core technology. "We have very strong patents that we defend," says Naughton. "Getting the patents was always among the first three issues on which we spent a lot of time and talent. We kept abreast of all the new inventions at universities.” <br />
<br />
However, the very same bioprospecting that made biotechnology so spectacularly successful cannot be said about its latest conception: synthetic biology. The life sciences' next transformative innovation, synthetic biology facilitates not just the transferring of segments of DNA among organisms but the complete redesign and construction of biological systems via entirely artificial genetic circuits from a toolkit of standardized biological parts. <br />
<br />
Through the rational and systematic design of biological systems to display functions not found in nature, synthetic biology promises to produce pharmaceuticals, cure cancer, and generate post-petroleum fuels- all remarkable assertions reminiscent of those made a generation ago at the start of the biotechnology boom. This time, however, inventors and entrepreneurs cannot simply rely on patents to facilitate industry growth. <br />
<br />
The emergence of such a new technology raises the question of whether the patent system, historically designed to protect mechanical and chemical products, is suitable to protect inventions in fields as new as synthetic biology. Adopting common practice in other engineering disciplines, synthetic biology standardizes genetic analogues to logic gates, oscillators, and circuit components. <br />
<br />
This process of standardization lays the template for the creation of truly artificial, man-made biological circuits to act in a rationally designed way. DNA is used to make parts; parts are assembled into devices; devices are assembled to make systems. This hierarchy in design introduces roadblocks in a product's path to commercialization. “A product generated by synthetic biology [...] can involve hundreds of different parts that [...] might all be protected by different patents that are probably held by several rights holders, thereby creating a so-called patent thicket,” wrote Berthold Rutz, a patent examiner specializing in synthetic biology. <br />
<br />
Specifically, as new technologies build upon old technologies, they become necessarily more complex, and as a result, are often subject to the protection of multiple patents, spanning both the new and old technologies. This effect, known as the tragedy of the anticommons, guarantees that everyone- the patent holders, manufacturers, and consumers- who could have benefited from the technology loses. <br />
<br />
Moreover, the interdisciplinary nature of synthetic biology requires patent offices to be proficient in a multitude of technical domains from computer science to nanotechnology, resulting not only in a shortage of synthetic biology examiners but also a general confusion and concern as to what constitutes patentable material. Rick Johnson, who heads up an OECD group on synthetic biology, has called the field “an IP law professor’s dream final examination problem,” arguing for a complete overhaul of US patent laws regarding the field. <br />
<br />
Legal experts also agree that something needs to be done. "Synthetic biology presents a particularly revealing example of a difficulty that the law has frequently faced-the assimilation of a new technology into... existing intellectual-property rights," wrote Arti Rai and James Boyle, from Duke University in North Carolina, in a recent paper. "The way that US law has handled software on the one hand and biotechnology on the other could come together in a 'perfect storm' that would impede the potential of the technology." <br />
<br />
The confusion clouding synthetic biology has led groups, such as Synthetic Genomics, founded by scientist and industrialist Craig Venter, to file patents on the very foundations and principles on which the field is built, potentially freezing progress in both research and commercialization by others. “Some of the patents being filed are astoundingly basic, the equivalent of patenting Boolean algebra right at the birth of computer science,” wrote Boyle. <br />
<br />
The disturbing ease of this sort of patenting has garnered the attention of synthetic biology's founding fathers. "You can take any device from the Texas instruments TTL catalogue, put 'genetically coded' in front of it without actually demonstrating it in practice, and you have a good chance of getting a patent," says Drew Endy, professor of bioengineering at Stanford. <br />
<br />
Tom Knight of MIT calls such patent claims, “absurdly, ridiculously broad.” The problem is aggravated by the fact that unlike in the biotechnology industry, the one molecule-one patent rule does not apply to synthetic biology. Instead, because it so intrinsically relies on a single standard upon which all future improvements are made, the field is particularly susceptible to patent trolls and entities who are seeking financial gain, but not pursuing product development. <br />
<br />
As it was throughout the biotechnology industry's growth, patents will play a key role in determinating the future of its latest product, synthetic biology. However, because of the inescapable differences between traditional biotechnology and synthetic biology, what was once a prime mover in the direction of innovation and commercialization has proven to be a double-edged sword. <br />
<br />
The premises originally established to stimulate progress may result in the very opposite effect. When synthetic biology's potential paths of innovation or stalemate are juxtaposed, there is an inescapable coupling of disappointment and appreciation of how patent law intersects our most promising sciences. Patents then, may indeed be our most transformative invention of all. <br />
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</html></div>McCoCohttp://2010.igem.org/Team:MIT_safetyTeam:MIT safety2010-10-27T18:12:21Z<p>McCoCo: </p>
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<div class="bodybaby">safety</div><br><br />
<h4>Would any of your project ideas raise safety issues in terms of:</h4><br />
<h5><ul><br />
<li>researcher safety</li><br />
<li>public safety, or</li><br />
<li>environmental safety?</li><br />
</ul></h5><br />
<hr><br />
<p>This summer, our team branched off into the bacterial team and the mammalian team. The bacterial team worked in a BSL1 lab setting, and the mammalian team worked in both a BSL1 and a BSL2 lab. Both teams adhered to <a href="http://www.who.int/csr/resources/publications/biosafety/en/Biosafety7.pdf">national</a> and <a href="http://ehs.mit.edu/site/content/biosafety-resources-and-links">local</a> safety protocols. <br />
Extra care was taken to not cross-contaminate lab spaces. A limited number of students worked with bacteriophage and mammalian cells. Work with phage and all materials involved with phage were confined to one fume hood, and the mammalian team worked in a BSL2 lab, separate from the bacterial team. Cross contamination from these settings were minimized by designating equipment specifically for phage, mammalian cells, and bacteria, as well as immediate change of personal protective equipment in moving between the different lab spaces.<br />
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<h4>Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? </h4><br />
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<p>Neither our bacteria nor our mammalian cells contain BioBrick parts that code for hazardous proteins or molecules.</p><br />
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<h4>Is there a local biosafety group, committee, or review board at your institution? </h4><br />
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<p>The <a href="http://ehs.mit.edu/site/">EHS (Environment, Health, and Safety) Office</a> is MIT's biosafety group that enforces lab safety in all labs on campus. They provide safety training, waste management services, and resources for safe lab practices. All undergraduates were trained by the EHS to work safely in BSL1 labs, and students working with mammalian cells underwent BSL2 lab safety training. Throughout the summer, the EHS helped us safely manage our biohazard waste. </p><br />
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<h4>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </h4><br />
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<dt><b>Parts</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_parts">All Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_composite">Composite</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_original">Original</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_backbones">Backbones</a></li><br />
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<table style="text-align: center;"><tr><td><br />
<a href="https://2010.igem.org/Team:MIT_psb1k15"><b>pSB1K15</b><br />
<br><img src="https://static.igem.org/mediawiki/2010/0/0b/L4_TRE_R1_image.jpg" width=200px></a></td><br />
<td><br />
<a href="https://2010.igem.org/Team:MIT_psb1k16"><b>pSB1k16</b><br />
<br><img src="https://static.igem.org/mediawiki/2010/3/33/L1_EGFP_L2_image.jpg" width=200px></a></td><br />
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<a href="https://2010.igem.org/Team:MIT_psb6c5"><b>K415049 and pSB6C5</b><br />
<br><img src="https://static.igem.org/mediawiki/2010/1/17/K415049.jpg" width=200px></a></td></table><br />
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</html></div>McCoCohttp://2010.igem.org/Team:MIT_originalTeam:MIT original2010-10-27T18:11:19Z<p>McCoCo: </p>
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<li><a href="https://2010.igem.org/Team:MIT_parts">All Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_composite">Composite</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_original">Original</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_backbones">Backbones</a></li><br />
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<a href="https://2010.igem.org/Team:MIT_K415300"><b>K415300</b><br><br />
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<a href="https://2010.igem.org/Team:MIT_k415301"><b>K415301</b><br><br />
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<a href="https://2010.igem.org/Team:MIT_k415031"><b>K415031</b><br><br />
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<a href="https://2010.igem.org/Team:MIT_k415032"><b>K415032</b><br><br />
<img height=50px src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br />
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<a href="https://2010.igem.org/Team:MIT_k415108"><b>K415108</b><br><br />
<img height=50px src="http://partsregistry.org/wiki/images/7/7c/K415100.png"></a><br><br />
</td><td colspan="1"><br />
<a href="https://2010.igem.org/Team:MIT_k415138"><b>K415138</b><br><br />
<img height=50px src="https://static.igem.org/mediawiki/2010/1/1f/K415138.png"></a><br><br />
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</html></div>McCoCohttp://2010.igem.org/Team:MIT_compositeTeam:MIT composite2010-10-27T18:10:45Z<p>McCoCo: </p>
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<li><a href="https://2010.igem.org/Team:MIT_parts">All Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_composite">Composite</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_original">Original</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_backbones">Backbones</a></li><br />
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<a href="https://2010.igem.org/Team:MIT_k415000"><b>BBa_K415000 <!--(AmpR PUC-19-pMB1)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/9/9e/K415000.png"></a><br />
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<a href="https://2010.igem.org/Team:MIT_k415006"><b>BBa_K415006 <!--(AmpR PUC-19-pMB1)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/e/e3/K415006.png"></a><br />
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<a href="https://2010.igem.org/Team:MIT_k415010"><b>BBa_K415010 <!--(AmpR PUC-19-pMB1)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/e/e4/K415010.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415019"><b>BBa_K415019 <!--(AmpR PUC-19-pMB1)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/b/bc/K415019.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415021"><b>BBa_K415021 <!--(KanR p15a)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/f/f8/K415021.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415022"><b>BBa_K415022 <!--(KanR p15a)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/6/6f/K415022.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415023"><b>BBa_K415023 <!--(KanR p15a)--!></b><br />
<br><img width=100% src="https://static.igem.org/mediawiki/igem.org/2/2d/K415023.png"></a><br />
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<a href="https://2010.igem.org/Team:MIT_k415069"><b>BBa_K415069 </b><br />
<br><img width=100% src="https://static.igem.org/mediawiki/2010/7/7d/K415069.png"></a><br />
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<a href="https://2010.igem.org/Team:MIT_k415151"><b>BBa_K415151 </b><br />
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</html></div>McCoCohttp://2010.igem.org/Team:MIT_partsTeam:MIT parts2010-10-27T18:10:09Z<p>McCoCo: </p>
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<dt><b style="width:180px;">Parts</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_parts">All Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_composite">Composite</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_original">Original</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_backbones">Backbones</a></li><br />
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<groupparts>iGEM010 MIT</groupparts></div>McCoCohttp://2010.igem.org/Team:MIT_summaryTeam:MIT summary2010-10-27T17:23:28Z<p>McCoCo: </p>
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<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
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<dt><b>Phage</b></dt><br />
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<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
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<dt><b>Mammalian</b></dt><br />
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<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
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<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">summary</div></td><br />
<tr><td><br><br />
<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/02/Overview-illustration_03.png" width=600px><br><br />
Living materials are powerful organic tools; they’re defined by their ability to adapt, to grow, to respond. Your skeleton becomes denser in regions of higher pressure; imagine a skyscraper or a bridge built of material with same capabilites!! <br><br><br />
<br />
When summer began, we imagined creating tiny biomaterial factories. We wanted to touch a plate of cells and watch them turn to bone. We envisioned a bacterial colony, sensing a pattern of radiation and synthesizing a 3D biomaterial composed only of interlinked particles of virus.<br><Br><br />
<br />
<b>So what have we accomplished towards these goals?</b><br><br />
<ul><li> We have a working synthetic circuit, capable of sensing radiation in bacterial cells, one that we’ve tinkered with to minimize cell death.<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/18/Screen_shot_2010-10-27_at_11.43.36_AM.png" width=600px><br />
</li><br />
<li>We’ve infected bacteria with modified phage to create ‘hairy’ cells; we’ve added pairs of linkers to phage proteins, that could allow them to polymerize.</li><br />
<li>In the mammalian system, we have cloned mechano-sensitive promoters and tested them in microfluidic devices.</li><br />
<li>We’ve induced stem cells to differentiate into bone.<br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/19/MammalianMaterial2.gif" width = 600px></li><br><br />
<li>We have a working synthetic circuit, capable of turning a small sensory pulse into a long term differentiation program. </li></ul><br><br />
<br />
This project is just the beginning; we’ve created the groundwork for a toolkit that anybody can use, one that will allow us to link sensory input to biomaterial creation. The system that we’ve built is meant to be expanded on, and our results can act as the basis for future exploration into of world of 3D organic material creation. </td><br />
</table><br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/File:MammalianMaterial2.gifFile:MammalianMaterial2.gif2010-10-27T17:22:56Z<p>McCoCo: </p>
<hr />
<div></div>McCoCohttp://2010.igem.org/Team:MIT_overviewTeam:MIT overview2010-10-27T15:53:12Z<p>McCoCo: </p>
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<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
<dt><b>Mammalian</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
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<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">abstract</div></td><br />
<tr><td><br>Materials technology is a rapidly advancing field with research focusing on new methods of nanomaterial design. The biggest problem with nanomaterials is that the creators (us) are on a completely different length scale when compared to the materials we venture to create. Our project strives to take small steps in the direction of nanomaterials by utilizing cells--both bacterial and mammalian--and bacteriophage as units in developing a living, self-assembling, dynamic biomaterial.<br />
<br><img src="https://static.igem.org/mediawiki/2010/0/02/Overview-illustration_03.png" width=100%><br><br />
Our goal is to create a system within cells that can convert a 2D design visible to the human eye into a 3D biostructure of phage or bone with the cross section of that same design. Our cells will be able to sense elements of the macro, human world, and output a living, self-assembled structure. Our mammalian team was motivated by the idea of the cellular “touch pad,” and is utilizing mechano-sensing promoters to allow mammalian cells to sense pressure and produce a controlled mineralization in response. The bacterial team is using the S.O.S. response from UV radiation and quorum sensing as stimuli to have bacteria secrete bacteriophage. Coated with zipper proteins, these bacteriophage can polymerize, cross-link and eventually form a living structure. Both teams are integrating a toggle switch into the system, allowing us to consistently control the cell’s response to the stimuli.<br />
<br><br><br />
By the end of the summer, we want our project to be able to showcase the capability of indirectly controlling the production of an organized biostructure. With the integration of multiple visible markers, user-directed design will be able to stimulate the production of a multichromatic output on a bacterial lawn, along with a tangible biostructure, and mechanical stimulation of our mammalian cell line will induce controlled differentiation of our cells into bone. We hope to have developed two systems with the ability to form <i>living</i> three-dimensional biomaterials that retain their ability to reform into a different structure if given the correct input. </td><br />
</table><br />
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</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_summaryTeam:MIT summary2010-10-27T15:52:20Z<p>McCoCo: </p>
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<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
<dt><b>Mammalian</b></dt><br />
<br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">summary</div></td><br />
<tr><td><br><br />
<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/02/Overview-illustration_03.png" width=600px><br><br />
Living materials are powerful organic tools; they’re defined by their ability to adapt, to grow, to respond. Your skeleton becomes denser in regions of higher pressure; imagine a skyscraper or a bridge built of material with same capabilites!! <br><br><br />
<br />
When summer began, we imagined creating tiny biomaterial factories. We wanted to touch a plate of cells and watch them turn to bone. We envisioned a bacterial colony, sensing a pattern of radiation and synthesizing a 3D biomaterial composed only of interlinked particles of virus.<br><Br><br />
<br />
<b>So what have we accomplished towards these goals?</b><br><br />
<ul><li> We have a working synthetic circuit, capable of sensing radiation in bacterial cells, one that we’ve tinkered with to minimize cell death.<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/18/Screen_shot_2010-10-27_at_11.43.36_AM.png" width=600px><br />
</li><br />
<li>We’ve infected bacteria with modified phage to create ‘hairy’ cells; we’ve added pairs of linkers to phage proteins, that could allow them to polymerize.</li><br />
<li>In the mammalian system, we have cloned mechano-sensitive promoters and tested them in microfluidic devices.</li><br />
<li>We’ve induced stem cells to differentiate into bone.<br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 600px></li><br><br />
<li>We have a working synthetic circuit, capable of turning a small sensory pulse into a long term differentiation program. </li></ul><br><br />
<br />
This project is just the beginning; we’ve created the groundwork for a toolkit that anybody can use, one that will allow us to link sensory input to biomaterial creation. The system that we’ve built is meant to be expanded on, and our results can act as the basis for future exploration into of world of 3D organic material creation. </td><br />
</table><br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_summaryTeam:MIT summary2010-10-27T15:51:59Z<p>McCoCo: </p>
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<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
<dt><b>Mammalian</b></dt><br />
<br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">summary</div></td><br />
<tr><td><br><br />
<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/02/Overview-illustration_03.png" width=500px><br><br />
Living materials are powerful organic tools; they’re defined by their ability to adapt, to grow, to respond. Your skeleton becomes denser in regions of higher pressure; imagine a skyscraper or a bridge built of material with same capabilites!! <br><br><br />
<br />
When summer began, we imagined creating tiny biomaterial factories. We wanted to touch a plate of cells and watch them turn to bone. We envisioned a bacterial colony, sensing a pattern of radiation and synthesizing a 3D biomaterial composed only of interlinked particles of virus.<br><Br><br />
<br />
<b>So what have we accomplished towards these goals?</b><br><br />
<ul><li> We have a working synthetic circuit, capable of sensing radiation in bacterial cells, one that we’ve tinkered with to minimize cell death.<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/18/Screen_shot_2010-10-27_at_11.43.36_AM.png" width=600px><br />
</li><br />
<li>We’ve infected bacteria with modified phage to create ‘hairy’ cells; we’ve added pairs of linkers to phage proteins, that could allow them to polymerize.</li><br />
<li>In the mammalian system, we have cloned mechano-sensitive promoters and tested them in microfluidic devices.</li><br />
<li>We’ve induced stem cells to differentiate into bone.<br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 600px></li><br><br />
<li>We have a working synthetic circuit, capable of turning a small sensory pulse into a long term differentiation program. </li></ul><br><br />
<br />
This project is just the beginning; we’ve created the groundwork for a toolkit that anybody can use, one that will allow us to link sensory input to biomaterial creation. The system that we’ve built is meant to be expanded on, and our results can act as the basis for future exploration into of world of 3D organic material creation. </td><br />
</table><br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_summaryTeam:MIT summary2010-10-27T15:51:33Z<p>McCoCo: </p>
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<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
<dt><b>Mammalian</b></dt><br />
<br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
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<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">summary</div></td><br />
<tr><td><br><br />
<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/02/Overview-illustration_03.png" width=500px><br />
Living materials are powerful organic tools; they’re defined by their ability to adapt, to grow, to respond. Your skeleton becomes denser in regions of higher pressure; imagine a skyscraper or a bridge built of material with same capabilites!! <br><br><br />
<br />
When summer began, we imagined creating tiny biomaterial factories. We wanted to touch a plate of cells and watch them turn to bone. We envisioned a bacterial colony, sensing a pattern of radiation and synthesizing a 3D biomaterial composed only of interlinked particles of virus.<br><Br><br />
<br />
<b>So what have we accomplished towards these goals?</b><br><br />
<ul><li> We have a working synthetic circuit, capable of sensing radiation in bacterial cells, one that we’ve tinkered with to minimize cell death.<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/18/Screen_shot_2010-10-27_at_11.43.36_AM.png" width=600px><br />
</li><br />
<li>We’ve infected bacteria with modified phage to create ‘hairy’ cells; we’ve added pairs of linkers to phage proteins, that could allow them to polymerize.</li><br />
<li>In the mammalian system, we have cloned mechano-sensitive promoters and tested them in microfluidic devices.</li><br />
<li>We’ve induced stem cells to differentiate into bone.<br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 600px></li><br><br />
<li>We have a working synthetic circuit, capable of turning a small sensory pulse into a long term differentiation program. </li></ul><br><br />
<br />
This project is just the beginning; we’ve created the groundwork for a toolkit that anybody can use, one that will allow us to link sensory input to biomaterial creation. The system that we’ve built is meant to be expanded on, and our results can act as the basis for future exploration into of world of 3D organic material creation. </td><br />
</table><br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/File:Overview-illustration_03.pngFile:Overview-illustration 03.png2010-10-27T15:50:40Z<p>McCoCo: </p>
<hr />
<div></div>McCoCohttp://2010.igem.org/Team:MIT_summaryTeam:MIT summary2010-10-27T15:47:30Z<p>McCoCo: </p>
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<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
<dt><b>Mammalian</b></dt><br />
<br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">summary</div></td><br />
<tr><td><br><br />
Living materials are powerful organic tools; they’re defined by their ability to adapt, to grow, to respond. Your skeleton becomes denser in regions of higher pressure; imagine a skyscraper or a bridge built of material with same capabilites!! <br><br><br />
<br />
When summer began, we imagined creating tiny biomaterial factories. We wanted to touch a plate of cells and watch them turn to bone. We envisioned a bacterial colony, sensing a pattern of radiation and synthesizing a 3D biomaterial composed only of interlinked particles of virus.<br><Br><br />
<br />
<b>So what have we accomplished towards these goals?</b><br><br />
<ul><li> We have a working synthetic circuit, capable of sensing radiation in bacterial cells, one that we’ve tinkered with to minimize cell death.<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/18/Screen_shot_2010-10-27_at_11.43.36_AM.png" width=600px><br />
</li><br />
<li>We’ve infected bacteria with modified phage to create ‘hairy’ cells; we’ve added pairs of linkers to phage proteins, that could allow them to polymerize.</li><br />
<li>In the mammalian system, we have cloned mechano-sensitive promoters and tested them in microfluidic devices.</li><br />
<li>We’ve induced stem cells to differentiate into bone.<br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 600px></li><br><br />
<li>We have a working synthetic circuit, capable of turning a small sensory pulse into a long term differentiation program. </li></ul><br><br />
<br />
This project is just the beginning; we’ve created the groundwork for a toolkit that anybody can use, one that will allow us to link sensory input to biomaterial creation. The system that we’ve built is meant to be expanded on, and our results can act as the basis for future exploration into of world of 3D organic material creation. </td><br />
</table><br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/File:Screen_shot_2010-10-27_at_11.43.36_AM.pngFile:Screen shot 2010-10-27 at 11.43.36 AM.png2010-10-27T15:44:54Z<p>McCoCo: </p>
<hr />
<div></div>McCoCohttp://2010.igem.org/Team:MIT_summaryTeam:MIT summary2010-10-27T15:44:17Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
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<head><br />
<style><br />
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background-color: #2e2e91;<br />
}<br />
#topnav li.academics ul {<br />
display: block;<br />
}<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/a/ae/Peacock_Drop.jpg');<br />
}<br />
</style><br />
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<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
<dt><b>Mammalian</b></dt><br />
<br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">summary</div></td><br />
<tr><td><br><br />
Living materials are powerful organic tools; they’re defined by their ability to adapt, to grow, to respond. Your skeleton becomes denser in regions of higher pressure; imagine a skyscraper or a bridge built of material with same capabilites!! <br><br><br />
<br />
When summer began, we imagined creating tiny biomaterial factories. We wanted to touch a plate of cells and watch them turn to bone. We envisioned a bacterial colony, sensing a pattern of radiation and synthesizing a 3D biomaterial composed only of interlinked particles of virus.<br><Br><br />
<br />
<b>So what have we accomplished towards these goals?</b><br><br />
<ul><li> We have a working synthetic circuit, capable of sensing radiation in bacterial cells, one that we’ve tinkered with to minimize cell death.</li><br />
<li>We’ve infected bacteria with modified phage to create ‘hairy’ cells; we’ve added pairs of linkers to phage proteins, that could allow them to polymerize.</li><br />
<li>In the mammalian system, we have cloned mechano-sensitive promoters and tested them in microfluidic devices.</li><br />
<li>We’ve induced stem cells to differentiate into bone.<br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 550px></li><br><br />
<li>We have a working synthetic circuit, capable of turning a small sensory pulse into a long term differentiation program. </li></ul><br><br />
<br />
This project is just the beginning; we’ve created the groundwork for a toolkit that anybody can use, one that will allow us to link sensory input to biomaterial creation. The system that we’ve built is meant to be expanded on, and our results can act as the basis for future exploration into of world of 3D organic material creation. </td><br />
</table><br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_summaryTeam:MIT summary2010-10-27T15:42:43Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
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<head><br />
<style><br />
#topnav li.academics a {<br />
background-color: #2e2e91;<br />
}<br />
#topnav li.academics ul {<br />
display: block;<br />
}<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/a/ae/Peacock_Drop.jpg');<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
<dt><b>Mammalian</b></dt><br />
<br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">summary</div></td><br />
<tr><td><br><br />
Living materials are powerful organic tools; they’re defined by their ability to adapt, to grow, to respond. Your skeleton becomes denser in regions of higher pressure; imagine a skyscraper or a bridge built of material with same capabilites!! <br><br><br />
<br />
When summer began, we imagined creating tiny biomaterial factories. We wanted to touch a plate of cells and watch them turn to bone. We envisioned a bacterial colony, sensing a pattern of radiation and synthesizing a 3D biomaterial composed only of interlinked particles of virus.<br><Br><br />
<br />
<b>So what have we accomplished towards these goals?</b><br><br />
<ul><li> We have a working synthetic circuit, capable of sensing radiation in bacterial cells, one that we’ve tinkered with to minimize cell death.</li><br />
<li>We’ve infected bacteria with modified phage to create ‘hairy’ cells; we’ve added pairs of linkers to phage proteins, that could allow them to polymerize.</li><br />
<li>In the mammalian system, we have cloned mechano-sensitive promoters and tested them in microfluidic devices.</li><br />
<li>We’ve induced stem cells to differentiate into bone.<br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 550px></li><br />
<li>We have a working synthetic circuit, capable of turning a small sensory pulse into a long term differentiation program. </li></ul><br><br />
<br />
This project is just the beginning; we’ve created the groundwork for a toolkit that anybody can use, one that will allow us to link sensory input to biomaterial creation. The system that we’ve built is meant to be expanded on, and our results can act as the basis for future exploration into of world of 3D organic material creation. </td><br />
</table><br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_summaryTeam:MIT summary2010-10-27T15:40:53Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
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background-color: #2e2e91;<br />
}<br />
#topnav li.academics ul {<br />
display: block;<br />
}<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/a/ae/Peacock_Drop.jpg');<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="nav"><br />
<dt><b>Bacteria</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li><br />
<li><a href="#">Characterization</a></li><br />
</ul><br />
</dd><br />
<dt><b>Phage</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li><br />
<br />
</ul><br />
</dd><br />
<dt><b>Mammalian</b></dt><br />
<br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby">summary</div></td><br />
<tr><td><br><br />
Living materials are powerful organic tools; they’re defined by their ability to adapt, to grow, to respond. Your skeleton becomes denser in regions of higher pressure; imagine a skyscraper or a bridge built of material with same capabilites!! <br><br><br />
<br />
When summer began, we imagined creating tiny biomaterial factories. We wanted to touch a plate of cells and watch them turn to bone. We envisioned a bacterial colony, sensing a pattern of radiation and synthesizing a 3D biomaterial composed only of interlinked particles of virus.<br><Br><br />
<br />
<b>So what have we accomplished towards these goals?</b><br><br />
<ul><li> We have a working synthetic circuit, capable of sensing radiation in bacterial cells, one that we’ve tinkered with to minimize cell death.</li><br />
<li>We’ve infected bacteria with modified phage to create ‘hairy’ cells; we’ve added pairs of linkers to phage proteins, that could allow them to polymerize.</li><br />
<li>In the mammalian system, we have cloned mechano-sensitive promoters and tested them in microfluidic devices.</li><br />
<li>We’ve induced stem cells to differentiate into bone.</li><br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 550px><br><br />
<li>We have a working synthetic circuit, capable of turning a small sensory pulse into a long term differentiation program. </li></ul><br><br />
<br />
This project is just the beginning; we’ve created the groundwork for a toolkit that anybody can use, one that will allow us to link sensory input to biomaterial creation. The system that we’ve built is meant to be expanded on, and our results can act as the basis for future exploration into of world of 3D organic material creation. </td><br />
</table><br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MITTeam:MIT2010-10-27T15:38:30Z<p>McCoCo: </p>
<hr />
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#topnav li.about ul {<br />
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background-image: url('https://static.igem.org/mediawiki/2010/d/d9/Whiteflowah.jpg');<br />
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</head><br />
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<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<a href="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" class="thickbox" title="The team. Not pictured, Crystal McKenzie, Arvind Thiagarajan, Lauren McGough, Jason Stevens."><img src="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" width=100%></a>The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.<br />
</div><br />
<br />
<br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; height: 700 px; margin-top:5px; padding: 10px;"><tr><td><div class="body">Programmable, Self-constructing Biomaterials</div></td><br />
<tr><td><br>The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.<br />
<div style="display:inline;"<a href="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" class="thickbox" title="Pretty materials. Ours are programmable."><img style="float: right; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" height=147px></a></div> <br />
<br><br><br />
We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.<br />
<br />
</td></table><br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MITTeam:MIT2010-10-27T15:34:11Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
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<html><br />
<head><br />
<title>MIT iGEM 2010</title><br />
<style><br />
#topnav li.about a {<br />
background-color: #8b0000;<br />
}<br />
#topnav li.about ul {<br />
display: block;<br />
}<br />
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#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/d/d9/Whiteflowah.jpg');<br />
background-repeat: no-repeat;<br />
}<br />
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</head><br />
<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<a href="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" class="thickbox" title="The team. Not pictured, Crystal McKenzie, Arvind Thiagarajan, Lauren McGough, Jason Stevens."><img src="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" width=100%></a>The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.<br />
</div><br />
<br />
<br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">Programmable, Self-constructing Biomaterials</div></td><br />
<tr><td><br>The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.<br />
<div style="display:inline;"<a href="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" class="thickbox" title="Pretty materials. Ours are programmable."><img style="float: right; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" height=147px></a></div> <br />
<br><br><br />
We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.<br />
<br />
<br><br><br />
<h5>Our Dynamic Living Materials</h5><br />
<br />
<strong>Mammalian</strong><br />
<hr><br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 500px><br />
<br><br />
<br><br><br><br />
<strong>Phage Material</strong><br />
<hr><br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/d/dc/Uvoverview.png" width = 500px><br />
<br><br />
<br />
<br />
</td></table><br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MITTeam:MIT2010-10-27T15:33:46Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
<br />
<html><br />
<head><br />
<title>MIT iGEM 2010</title><br />
<style><br />
#topnav li.about a {<br />
background-color: #8b0000;<br />
}<br />
#topnav li.about ul {<br />
display: block;<br />
}<br />
<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/d/d9/Whiteflowah.jpg');<br />
background-repeat: no-repeat;<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<a href="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" class="thickbox" title="The team. Not pictured, Crystal McKenzie, Arvind Thiagarajan, Lauren McGough, Jason Stevens."><img src="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" width=100%></a>The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.<br />
</div><br />
<br />
<br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">Programmable, Self-constructing Biomaterials</div></td><br />
<tr><td><br>The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.<br />
<div style="display:inline;"<a href="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" class="thickbox" title="Pretty materials. Ours are programmable."><img style="float: right; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" height=147px></a></div> <br />
<br><br><br />
We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.<br />
<br />
<br><br><br />
<h5>Our Dynamic Living Materials</h5><br />
<br />
<strong>Mammalian</strong><br />
<hr><br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 500px><br />
<br><br />
<br />
<strong>Phage Material</strong><br />
<hr><br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/d/dc/Uvoverview.png" width = 500px><br />
<br><br />
<br />
<br />
</td></table><br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MITTeam:MIT2010-10-27T15:32:41Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
<br />
<html><br />
<head><br />
<title>MIT iGEM 2010</title><br />
<style><br />
#topnav li.about a {<br />
background-color: #8b0000;<br />
}<br />
#topnav li.about ul {<br />
display: block;<br />
}<br />
<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/d/d9/Whiteflowah.jpg');<br />
background-repeat: no-repeat;<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<a href="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" class="thickbox" title="The team. Not pictured, Crystal McKenzie, Arvind Thiagarajan, Lauren McGough, Jason Stevens."><img src="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" width=100%></a>The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.<br />
</div><br />
<br />
<br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">Programmable, Self-constructing Biomaterials</div></td><br />
<tr><td><br>The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.<br />
<div style="display:inline;"<a href="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" class="thickbox" title="Pretty materials. Ours are programmable."><img style="float: right; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" height=147px></a></div> <br />
<br><br><br />
We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.<br />
<br />
<br><br><br />
<h5>Our Dynamic Living Materials</h5><br />
<br />
<strong>Mammalian</strong><br />
<hr><br><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 500px><br />
<br><br />
<br />
<strong>Phage Material</strong><br />
<br />
<br />
</td></table><br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MITTeam:MIT2010-10-27T15:27:35Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
<br />
<html><br />
<head><br />
<title>MIT iGEM 2010</title><br />
<style><br />
#topnav li.about a {<br />
background-color: #8b0000;<br />
}<br />
#topnav li.about ul {<br />
display: block;<br />
}<br />
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#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/d/d9/Whiteflowah.jpg');<br />
background-repeat: no-repeat;<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<a href="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" class="thickbox" title="The team. Not pictured, Crystal McKenzie, Arvind Thiagarajan, Lauren McGough, Jason Stevens."><img src="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" width=100%></a>The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.<br />
</div><br />
<br />
<br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">Programmable, Self-constructing Biomaterials</div></td><br />
<tr><td><br>The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.<br />
<div style="display:inline;"<a href="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" class="thickbox" title="Pretty materials. Ours are programmable."><img style="float: right; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" height=147px></a></div> <br />
<br><br><br />
We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.<br />
<br />
<br><br><br />
<h5>Our Dynamic Living Materials</h5><br />
<br />
<strong>Mammalian</strong><br />
<hr><br<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 500px><br />
<br><br />
<br />
</td></table><br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MITTeam:MIT2010-10-27T15:27:07Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
<br />
<html><br />
<head><br />
<title>MIT iGEM 2010</title><br />
<style><br />
#topnav li.about a {<br />
background-color: #8b0000;<br />
}<br />
#topnav li.about ul {<br />
display: block;<br />
}<br />
<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/d/d9/Whiteflowah.jpg');<br />
background-repeat: no-repeat;<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<a href="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" class="thickbox" title="The team. Not pictured, Crystal McKenzie, Arvind Thiagarajan, Lauren McGough, Jason Stevens."><img src="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" width=100%></a>The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.<br />
</div><br />
<br />
<br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">Programmable, Self-constructing Biomaterials</div></td><br />
<tr><td><br>The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.<br />
<div style="display:inline;"<a href="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" class="thickbox" title="Pretty materials. Ours are programmable."><img style="float: right; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" height=147px></a></div> <br />
<br><br><br />
We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.<br />
<br />
<br><br><br />
<h5>Our Dynamic Living Materials</h5><br />
<br />
<strong>Mammalian</strong><br />
<hr><br<br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 300px><br />
<br><br />
<br />
</td></table><br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MITTeam:MIT2010-10-27T15:26:30Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
<br />
<html><br />
<head><br />
<title>MIT iGEM 2010</title><br />
<style><br />
#topnav li.about a {<br />
background-color: #8b0000;<br />
}<br />
#topnav li.about ul {<br />
display: block;<br />
}<br />
<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/d/d9/Whiteflowah.jpg');<br />
background-repeat: no-repeat;<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<a href="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" class="thickbox" title="The team. Not pictured, Crystal McKenzie, Arvind Thiagarajan, Lauren McGough, Jason Stevens."><img src="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" width=100%></a>The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.<br />
</div><br />
<br />
<br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">Programmable, Self-constructing Biomaterials</div></td><br />
<tr><td><br>The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.<br />
<div style="display:inline;"<a href="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" class="thickbox" title="Pretty materials. Ours are programmable."><img style="float: right; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" height=147px></a></div> <br />
<br><br><br />
We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.<br />
<br />
<br><br><br />
<h5>Our Dynamic Living Materials</h5><br />
<br />
<strong>Mammalian</strong><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif" width = 300px><br />
<br />
</td></table><br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MITTeam:MIT2010-10-27T15:26:01Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
<br />
<html><br />
<head><br />
<title>MIT iGEM 2010</title><br />
<style><br />
#topnav li.about a {<br />
background-color: #8b0000;<br />
}<br />
#topnav li.about ul {<br />
display: block;<br />
}<br />
<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/d/d9/Whiteflowah.jpg');<br />
background-repeat: no-repeat;<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<a href="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" class="thickbox" title="The team. Not pictured, Crystal McKenzie, Arvind Thiagarajan, Lauren McGough, Jason Stevens."><img src="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" width=100%></a>The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.<br />
</div><br />
<br />
<br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">Programmable, Self-constructing Biomaterials</div></td><br />
<tr><td><br>The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.<br />
<div style="display:inline;"<a href="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" class="thickbox" title="Pretty materials. Ours are programmable."><img style="float: right; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" height=147px></a></div> <br />
<br><br><br />
We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.<br />
<br />
<br><br><br />
<h5>Our Dynamic Living Materials</h5><br />
<br />
<strong>Mammalian</strong><br />
<img style="float: left; padding: 10px" src="https://static.igem.org/mediawiki/2010/1/13/MammalianAnimation.gif"><br />
<br />
</td></table><br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/File:MammalianAnimation.gifFile:MammalianAnimation.gif2010-10-27T15:16:02Z<p>McCoCo: </p>
<hr />
<div></div>McCoCohttp://2010.igem.org/File:Screen_shot_2010-10-22_at_9.45.25_PM.pngFile:Screen shot 2010-10-22 at 9.45.25 PM.png2010-10-27T13:37:46Z<p>McCoCo: </p>
<hr />
<div></div>McCoCohttp://2010.igem.org/Team:MIT_backbonesTeam:MIT backbones2010-10-27T08:37:02Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
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<html><br />
<head><br />
<style><br />
#topnav li.notebook a {<br />
background-color: #016b9d;<br />
}<br />
#topnav li.notebook ul {<br />
display: block;<br />
}<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/3/3b/Blueflowah.jpg');<br />
background-attachment: fixed;<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header" ><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="specialnav"><br />
<dt><b>Parts</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_parts">All Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_composite">Composite</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_original">Original</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_backbones">Backbones</a></li><br />
</ul><br />
</dd><br />
</dl><br />
<dl id="nav"><br />
<dt><b>Data</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_gels">Gels</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_experimental">Experimental</a></li><br />
<br />
</ul><br />
</dd><br />
<br />
</dl><br />
</div><br />
<br />
<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><br />
<tr><td><div class="bodybaby">New Standard Backbones</div></td><br />
<tr><td><br><br />
<br />
<table style="text-align: center;"><tr><td><br />
<a href="https://2010.igem.org/Team:MIT_psb1k15"><b>pSB1K15</b><br />
<br><img src="https://static.igem.org/mediawiki/2010/0/0b/L4_TRE_R1_image.jpg" width=200px></a></td><br />
<td><br />
<a href="https://2010.igem.org/Team:MIT_psb1k16"><b>pSB1k16</b><br />
<br><img src="https://static.igem.org/mediawiki/2010/3/33/L1_EGFP_L2_image.jpg" width=200px></a></td><br />
<td><br />
<a href="https://2010.igem.org/Team:MIT_psb6c5"><b>pSB6C5</b><br />
<br><img src="https://static.igem.org/mediawiki/2010/b/b1/Plasmidz_yo.png" width=200px></a></td></table><br />
<br><br />
<br />
<br />
<br />
</td><br />
</table><br />
<br />
<br />
<br />
<br />
</div><br />
</div><br />
</body><br />
</html></div>McCoCohttp://2010.igem.org/Team:MIT_originalTeam:MIT original2010-10-27T08:36:28Z<p>McCoCo: </p>
<hr />
<div>{{CM_css}}<br />
<br />
<html><br />
<head><br />
<style><br />
#topnav li.notebook a {<br />
background-color: #016b9d;<br />
}<br />
#topnav li.notebook ul {<br />
display: block;<br />
}<br />
#content {<br />
background-image: url('https://static.igem.org/mediawiki/2010/3/3b/Blueflowah.jpg');<br />
background-attachment: fixed;<br />
}<br />
</style><br />
</head><br />
<body><br />
<div class="header" ><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<dl id="specialnav"><br />
<dt><b>Parts</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_parts">All Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_composite">Composite</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_original">Original</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_backbones">Backbones</a></li><br />
</ul><br />
</dd><br />
</dl><br />
<dl id="nav"><br />
<dt><b>Data</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_gels">Gels</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_experimental">Experimental</a></li><br />
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</ul><br />
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<tr><td><br />
<div class="bodybaby">Original Parts</div><br />
<br />
<center><table style="text-align:center"><tr><br />
<td colspan="2"><br />
<a href="https://2010.igem.org/Team:MIT_K415300"><b>K415300</b><br><br />
<img src="https://static.igem.org/mediawiki/2010/f/f4/Ptsma.png" width=200px></a><br><br />
</td><td colspan="2"><br />
<a href="https://2010.igem.org/Team:MIT_k415301"><b>K415301</b><br><br />
<img width=190px src="https://static.igem.org/mediawiki/2010/1/1e/Plpta.png"></a><br><br />
</td><br />
<tr><td colspan="1"><br />
<a href="https://2010.igem.org/Team:MIT_k415031"><b>K415031</b><br><br />
<img height=50px src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br />
</td><td colspan="1"><br />
<a href="https://2010.igem.org/Team:MIT_k415032"><b>K415032</b><br><br />
<img height=50px src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br />
</td><td colspan="1"><br />
<a href="https://2010.igem.org/Team:MIT_k415108"><b>K415108</b><br><br />
<img height=50px src="http://partsregistry.org/wiki/images/7/7c/K415100.png"></a><br><br />
</td><td colspan="1"><br />
<a href="https://2010.igem.org/Team:MIT_k415138"><b>K415138</b><br><br />
<img height=50px src="https://static.igem.org/mediawiki/2010/1/1f/K415138.png"></a><br><br />
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</html></div>McCoCohttp://2010.igem.org/Team:MIT_compositeTeam:MIT composite2010-10-27T08:35:56Z<p>McCoCo: </p>
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<dl id="specialnav"><br />
<dt><b>Parts</b></dt><br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_parts">All Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_composite">Composite</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_original">Original</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_backbones">Backbones</a></li><br />
</ul><br />
</dd><br />
</dl><br />
<dl id="nav"><br />
<dt><b>Data</b></dt><br />
<br />
<dd><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:MIT_gels">Gels</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_experimental">Experimental</a></li><br />
<br />
</ul><br />
</dd><br />
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</dl><br />
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<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><br />
<tr><td><div class="bodybaby">new composite biobricks</div></td><br />
<tr><td><br><br />
<a href="https://2010.igem.org/Team:MIT_k415000"><b>BBa_K415000 <!--(AmpR PUC-19-pMB1)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/9/9e/K415000.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415006"><b>BBa_K415006 <!--(AmpR PUC-19-pMB1)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/e/e3/K415006.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415010"><b>BBa_K415010 <!--(AmpR PUC-19-pMB1)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/e/e4/K415010.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415019"><b>BBa_K415019 <!--(AmpR PUC-19-pMB1)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/b/bc/K415019.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415021"><b>BBa_K415021 <!--(KanR p15a)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/f/f8/K415021.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415022"><b>BBa_K415022 <!--(KanR p15a)--!></b><br />
<br><img src="https://static.igem.org/mediawiki/igem.org/6/6f/K415022.png"></a><br />
<br><br><br />
<a href="https://2010.igem.org/Team:MIT_k415023"><b>BBa_K415023 <!--(KanR p15a)--!></b><br />
<br><img width=100% src="https://static.igem.org/mediawiki/igem.org/2/2d/K415023.png"></a><br />
<a href="https://2010.igem.org/Team:MIT_k415069"><b>BBa_K415069 </b><br />
<br><img width=100% src="https://static.igem.org/mediawiki/2010/7/7d/K415069.png"></a><br />
<a href="https://2010.igem.org/Team:MIT_k415151"><b>BBa_K415151 </b><br />
<br><img width=100% src="https://static.igem.org/mediawiki/2010/d/d4/K415151.png"></a><br />
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</html></div>McCoCohttp://2010.igem.org/Team:MIT_undergraduatesTeam:MIT undergraduates2010-10-27T08:16:17Z<p>McCoCo: </p>
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<div>{{CM_css}}<br />
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#topnav li.people a {<br />
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background-image: url('https://static.igem.org/mediawiki/2010/4/44/Orangeflowah.jpg');<br />
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<div class="header"><br />
<br />
<div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"><br />
<a href="https://static.igem.org/mediawiki/2010/a/a7/Student.JPG" class="thickbox"><img width=100% src="https://static.igem.org/mediawiki/2010/a/a7/Student.JPG"></a>Paul pondering.<br />
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<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"><br />
<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><br />
<div class="bodybaby" style="width: 70%;">the undergraduates</div><br />
<ul id="freedom" class="idTabs"> <br />
<li><a class="selected" href="#Andrew">Andrew</a></li> <br />
<li><a href="#Arvind">Arvind</a></li> <br />
<li><a href="#Crystal">Crystal</a></li> <br />
<li><a href="#Grant">Grant</a></li> <br />
<li><a href="#Jason">Jason</a></li> <br />
<li><a href="#Joy">Joy</a></li><br />
<li><a href="#Kevinw">Kevin</a></li><br />
<li><a href="#Laura">Laura</a></li><br />
<li><a href="#Lauren">Lauren</a></li><br />
<li><a href="#Leanna">Leanna</a></li><br />
<li><a href="#Paul">Paul</a></li><br />
<li><a href="#Shawn">Shawn</a></li><br />
<li><a href="#Shirley">Shirley</a></li><br />
</ul> <br />
<br />
<div class="tabContainer" style="width: 500px; padding: 10px; display: block; float:left; position: relative; top: -325px; left: 110px; border: none;"> <br />
<p id="Andrew"><a href="https://static.igem.org/mediawiki/2010/9/95/080210Andrew.jpg" class="thickbox" title="He's the tallest asian I've ever met."><img src="https://static.igem.org/mediawiki/2010/9/95/080210Andrew.jpg" style="display: inline; float:left; padding-right: 10px;" height=300px width=225px></a>This is Andrew. He's pretty much a <del>baller</del> joke. Nah, just messin'. Andrew, better known to the team as Andre, has an intense bromance with another team member, Paul Muir.<br />
</p> <br />
<p id="Arvind"><a href="https://static.igem.org/mediawiki/2010/e/eb/080210Arvind.jpg" class="thickbox" title="He's the secret weapon."><img src="https://static.igem.org/mediawiki/2010/e/eb/080210Arvind.jpg" style="display: inline; float:left; padding-right: 10px;" height=225px width=300px></a>This is Arvind. He's our secret weapon!<br />
</p> <br />
<p id="Crystal"><a href="https://static.igem.org/mediawiki/2010/e/ed/Crystal.jpg" class="thickbox" title="Bakes the best cookies ever."><img src="https://static.igem.org/mediawiki/2010/e/ed/Crystal.jpg" style="display: inline; float:left; padding-right: 10px;"width=200px"></a>This is Crystal. She is a rising junior in Course 10. <br />
</p><br />
<p id="Grant"><a href="https://static.igem.org/mediawiki/2010/8/88/080210Grant.jpg" class="thickbox" title="Mug shot."><img src="https://static.igem.org/mediawiki/2010/8/88/080210Grant.jpg" style="display: inline; float:left; padding-right: 10px;" height=225px width=250px></a>This is Grant :cough: ...I mean, Abdul. As you can tell, he's a pretty scary guy. Fun fact: Toward the middle of the summer, Grant started literally living in the lab.<br />
</p><br />
<p id="Jason"><a href="https://static.igem.org/mediawiki/2010/f/f0/080210Jason.jpg" class="thickbox" title="Todo..."><img src="https://static.igem.org/mediawiki/2010/f/f0/080210Jason.jpg" style="display: inline; float:left; padding-right: 10px;" height=300px width=210px></a>This is Jstev. He's a rising senior from... wait for it... the University of Kansas! But we consider him to be an honorary MIT baller. Jstev gets paid more than we do and also has free housing. :( Chances are that Jstev is older than you, and he is known to some as Mr. Potatohead. <br />
<br />
...JSTEV!!!<br />
</p><br />
<p id="Joy"><a href="https://static.igem.org/mediawiki/2010/f/f1/080210Joy.jpg" class="thickbox" title="MSC expert"><img src="https://static.igem.org/mediawiki/2010/f/f1/080210Joy.jpg" style="display: inline; float:left; padding-right: 10px;" height=300px width=200px></a>This is Joy. She's Asian!<br />
</p><br />
<p id="Kevinw"><a href="https://static.igem.org/mediawiki/2010/4/47/Kevinw.jpg" class="thickbox" title="Highschooler."><img src="https://static.igem.org/mediawiki/2010/4/47/Kevinw.jpg" style="display: inline; float:left; padding-right: 10px;" height=200px></a>This is Kevin. He's a minor!<br />
</p><br />
<p id="Laura"><a href="https://static.igem.org/mediawiki/2010/f/f9/080210Laura.jpg" class="thickbox" title="Mammalian Champ."><img src="https://static.igem.org/mediawiki/2010/f/f9/080210Laura.jpg" style="display: inline; float:left; padding-right: 10px;" height=250px width=200px></a>This is Laura. She works with mammals! <br />
</p><br />
<p id="Lauren"><a href="http://sphotos.ak.fbcdn.net/hphotos-ak-snc4/hs700.snc4/62018_1434380899738_1239390081_31193707_5264798_n.jpg" class="thickbox" title="Computationer."><img src="http://sphotos.ak.fbcdn.net/hphotos-ak-snc4/hs700.snc4/62018_1434380899738_1239390081_31193707_5264798_n.jpg" style="display: inline; float:left; padding-right: 10px;" height=200px></a>This is Lauren. She's Course 6.<br />
</p><br />
<p id="Leanna"><a href="https://static.igem.org/mediawiki/2010/9/98/Leanna.jpg" class="thickbox" title="blahh"><img src="https://static.igem.org/mediawiki/2010/9/98/Leanna.jpg" style="display: inline; float:left; padding-right: 10px;" height=300px width=160px></a>This is Leanna. She is a rising junior in Course 20. She likes gymnastics and NCAA basketball (men's) and has an intense passion for the Situation.<br />
</p><br />
<p id="Paul"><a href="https://static.igem.org/mediawiki/2010/a/a3/Paul.jpg" class="thickbox" title="Fanboy."><img src="https://static.igem.org/mediawiki/2010/a/a3/Paul.jpg" style="display: inline; float:left; padding-right: 10px;" height=300px width=225px></a>This is Paul. He lived in Switzerland for years, and yes, he's related to John Muir. <br />
</p><br />
<p id="Shawn"><a href="https://static.igem.org/mediawiki/2010/5/5f/080210Shawn.jpg" class="thickbox" title="Does everything."><img src="https://static.igem.org/mediawiki/2010/5/5f/080210Shawn.jpg" style="display: inline; float:left; padding-right: 10px;" height=300px width=225px></a>This is Shawn. His unorthodox protocols may one day culminate in several BioBrick standards.<br />
</p><br />
<p id="Shirley"><a href="https://static.igem.org/mediawiki/2010/e/ef/080210Shirley.jpg" class="thickbox" title="Phage!!"><img src="https://static.igem.org/mediawiki/2010/e/ef/080210Shirley.jpg" style="display: inline; float:left; padding-right: 10px;" height=300px width=225px></a>This is Shirley. She's a vegan, but apparently wouldn't mind feasting on her fellow humans. She also enjoys the artist Joanna Newsom, implying she has a few screws loose in her head. Most importantly, however, Shirley is *hungry.*<br />
</p></div><br />
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