Restriction Enzyme Digests

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Revision as of 19:44, 16 October 2010 by Bzhang89 (Talk | contribs)

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http://openwetware.org/wiki/Knight:Restriction_Digest

http://openwetware.org/wiki/Endy:Restriction_Digest

After heat-killing restriction enzymes, add dpni to rid of background plasmids (circular template plasmids that are supposed to be linearized) - dpni works in most buffers

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