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Restriction Enzyme Digests
From 2010.igem.org
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After heat-killing restriction enzymes, add dpni to rid of background plasmids (circular template plasmids that are supposed to be linearized) - dpni works in most buffers | After heat-killing restriction enzymes, add dpni to rid of background plasmids (circular template plasmids that are supposed to be linearized) - dpni works in most buffers | ||
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| + | Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</span>]] | ||
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| + | [[Category:Protocol]] | ||
Revision as of 19:44, 16 October 2010
CHOOOSE
http://openwetware.org/wiki/Knight:Restriction_Digest
http://openwetware.org/wiki/Endy:Restriction_Digest
After heat-killing restriction enzymes, add dpni to rid of background plasmids (circular template plasmids that are supposed to be linearized) - dpni works in most buffers
Back to Protocol
