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Reagents
From 2010.igem.org
(New page: Ampicillin - sodium salt Sigma A95180-5G $50.30 Chloramphenicol - Sigma C0378-5G $29.00 Kanamycin - Sigma K4000-5G $59.60 Tetracycline hydochloride - Sigma T3383-25G $47.50 USA Scientifi...) |
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600630 Pfu Ultra Hotstart PCR Master Mix $357.00 | 600630 Pfu Ultra Hotstart PCR Master Mix $357.00 | ||
This is for 100 rxn - smallest size maybe there is something cheaper. | This is for 100 rxn - smallest size maybe there is something cheaper. | ||
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| + | The polymerase that I enjoy is called Phusion. It is a NEB polymerase, but a little pricey. If you are using small (<5kb) plasmids, I've had luck with Vent (also NEB). Peggy would be a good person to ask about this. -THUS SPAKE AVI | ||
Revision as of 02:59, 29 June 2010
Ampicillin - sodium salt Sigma A95180-5G $50.30 Chloramphenicol - Sigma C0378-5G $29.00 Kanamycin - Sigma K4000-5G $59.60 Tetracycline hydochloride - Sigma T3383-25G $47.50
USA Scientific PO Box 3565 Ocala, FL 34478 ph 800-522-8477 fax 352-351-2057 FEIN V592244579 A for tips 1 BG 1111-1006 Yellow beveled tips, bulk bag $10.80 1 BG 1111-2021 blue tips , bulk bag $12.95 1 BG 1111-3000 clear tips (1-10 ul) bulk bag
LabSource
7350 S. Madison Street
Willowbrook, IL 60527
ph 630 413 5662
fax 630 325 5662
FEIN V363631684 1
Vendor Note: toll free 800-545-8823
Microcentrifuge tubes
BX T56-950 1.5 ml boil proof microcentrifuge tubes $27.95
Agilent Technologies P.O. Box 75265 Charlotte, NC 28275-0265 ph 800-227-9770 fax 302-633-8954 FEIN V770518772 E for Taq polymerase 600630 Pfu Ultra Hotstart PCR Master Mix $357.00 This is for 100 rxn - smallest size maybe there is something cheaper.
The polymerase that I enjoy is called Phusion. It is a NEB polymerase, but a little pricey. If you are using small (<5kb) plasmids, I've had luck with Vent (also NEB). Peggy would be a good person to ask about this. -THUS SPAKE AVI
