Protocol/8

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TEAM ALBERTA

Protocol 8: Ligation


What you will need:

  • Vector
  • Insert
  • Milli Q buffer
  • 5X ligase buffer
  • T4 DNA ligase

Volumes of vector and insert may vary but a typical 20 ul reaction might be

milliQ water 11 ul
5x ligase buffer 4 ul
vector (200 ng/ul) 1 ul
insert (200 ng/ul) 3 ul
ligase 1 ul

Incubate at R/T for 1 hrs or overnight at 12-16 C


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