From 2010.igem.org
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| - | Protocol 4: Plasmid Miniprep
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| - | What you will need:
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| - | * Overnight culture
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| - | * Qiagen plasmid miniprep kit
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| - | Procedure:
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| - | * Transfer 1.5 ml of culture to Eppendorf.
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| - | * Spin down at 13 000 rpm.
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| - | * Discard supernatant and follow procedure on p. 22 of [http://www.qiagen.com/literature/render.aspx?id=370 QIAprep Miniprep Handbook].
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| - | * Determine concentration of purified plasmid with nanodrop
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| - | ==Notes==
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| - | * Low speed pellet is easier to resuspend.
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| - | * To resuspend, take an empty micro centrifuge rack and rub the tube quickly over the holes, so it makes a "quacking" sound. It's pretty fun and works pretty good. Vortex after too.
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| - | * Step 2 in Qiagen, the alkaline lysis with buffer P2, should be brief to avoid damaging DNA.
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| - | [[Team:Alberta/Notebook/protocols| Back]]
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Latest revision as of 17:16, 26 October 2010
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