From 2010.igem.org
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| - | Protocol 3: Glycerol Stock
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| - | Reagents:
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| - | * Overnight with bacterial growth (probably 1.5mL)
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| - | * Screw cap tubes
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| - | * Glycerol
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| - | Procedure:
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| - | * Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
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| - | * Add 350uL of overnight culture to each tube.
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| - | * Pipet up and down to gently mix.
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| - | * Flash freeze in liquid N2 or dry ice/methanol bath.
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| - | * Place in -80<sup>o</sup>C freezer when frozen.
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| - | Notes:
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| - | * If we make extra we have them if we need to send them out or want to store in several locations.
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| - | [[Team:Alberta/Notebook/protocols| Back]]
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| - | {{Team:Alberta/endMainContent}}
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Latest revision as of 17:16, 26 October 2010
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