Protocol/3

From 2010.igem.org

(Difference between revisions)
Line 14: Line 14:
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What you will need:
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Reagents:
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* Overnight with bacterial growth (probably 1.5 mL)
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* Overnight with bacterial growth (probably 1.5mL)
* Screw cap tubes
* Screw cap tubes
* Glycerol  
* Glycerol  
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Procedure:
Procedure:
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* Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
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* Pipet 150 uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
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* Add 350uL of overnight culture to each tube.
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* Add 350 uL of overnight culture to each tube.
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* Pipet up and down to gently mix.
* Pipet up and down to gently mix.
* Flash freeze in liquid N2 or dry ice/methanol bath
* Flash freeze in liquid N2 or dry ice/methanol bath
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* Place in -80 C freezer when frozen.
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* Place in -80<sup>o</sup>C freezer when frozen.
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Notes:
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==Notes==
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* If we make extra we have them if we need to send them out or want to store in several locations.
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* If we make extra we have them if we need to send them out or want to store in several locations.
 
[[Team:Alberta/Notebook/protocols| Back]]
[[Team:Alberta/Notebook/protocols| Back]]
{{Team:Alberta/endMainContent}}
{{Team:Alberta/endMainContent}}

Revision as of 01:02, 26 October 2010

TEAM ALBERTA

Protocol 3: Glycerol Stock


Reagents:

  • Overnight with bacterial growth (probably 1.5mL)
  • Screw cap tubes
  • Glycerol


Procedure:

  • Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
  • Add 350uL of overnight culture to each tube.
  • Pipet up and down to gently mix.
  • Flash freeze in liquid N2 or dry ice/methanol bath
  • Place in -80oC freezer when frozen.

Notes:

  • If we make extra we have them if we need to send them out or want to store in several locations.


Back