"
Protocol/0
From 2010.igem.org
Contents |
Metaprotocol for adding and modifying University of Alberta iGEM protocols
Abstract
This protocol (or metaprotocol really, since it is a protocol for protocols) provides 1) a template for iGEM lab protocols and 2) a process for creating and modifying those protocols.
Materials
The Materials section should list everything necessary to perform the protocol here. Include all information about suppliers, ordering details, etc. Links to the suppliers' page on that material are also appropriate and encouraged. Protocol 0 requires no materials.
Reagents
The Reagents section lists biological resources e.g. cell lines, buffers (link to a method for making them), enzymes, chemicals, kits, etc. Protocol 0 requires no reagents.
Equipment
The Equipment section lists equipment used to perform the protocol (link to a method for using them). Protocol 0 requires a computer linked to the World Wide Web.
Procedure
The procedure section contains a step by step guide to the experimental procedure.
New protocol
To create a new protocol, carry out the following steps:
- add an entry to the list of protocols, giving your protocol a name and a number
- copy the source from this protocol and paste it into your new protocol
- modify every section
Modify an existing protocol
Click "edit", and make changes. If they are substantive, add an explanation to the History section.
Critical steps
Referenced from the main protocol, a more thorough explanation of particularly important steps in the protocol.
Troubleshooting
Referenced from the main protocol, an explanation of what can cause things to go wrong with the protocol.
Notes
Referenced from the main protocol, any comments about the protocol should be made here; i.e. how it was developed. Any comments added should be signed (by adding *'''~~~~''': in front) and explained. Links to FAQs/tips provided by other sources such as the manufacturer or other websites would be best made here.
Anecdotal observations that might be of use to others can also be posted here; e.g. 'my cells were still floating'.
It might also be good to add an image to show the workflow and timescales for experiment planning.
Acknowledgments
Acnkowledge any help you had in development, testing, writing this protocol. The template was stolen wholesale from openwetware.org.
References
See OpenWetWare:Biblio for information on how to reference within a wiki.
Specific Protocols
Add links to all the OWW protocols that have been used in making the consensus.
Author(s) and Revision History
Initial version --Dridgway 16:34, 19 March 2009 (MDT)
