O/N Culture

From 2010.igem.org

Revision as of 17:36, 14 June 2010 by Asamelson (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


  • Take 5mL LB and put in a round bottom tube
  • If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube MAKE SURE YOU ADD THE RIGHT ONE!
  • Take a tip on a p200 and lightly touch a colony
  • Eject the tip into the culture and put the cap on
  • For a miniprep, grow O/N for 12-16 hours NOT LONGER