O/N Culture

From 2010.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 8: Line 8:
*Eject the tip into the culture and put the cap on
*Eject the tip into the culture and put the cap on
*For a miniprep, grow O/N for 12-16 hours NOT LONGER
*For a miniprep, grow O/N for 12-16 hours NOT LONGER
-
[http://openwetware.org/wiki/Bacterial_cell_culture]
+
[http://openwetware.org/wiki/Bacterial_cell_culture]Openwetware
 +
 
 +
Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</span>]]
 +
 
 +
[[Category:Protocol]]

Latest revision as of 19:24, 16 October 2010

REMEMBER TO BE STERILE


  • Take 5mL LB and put in a round bottom tube
  • If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube
  *MAKE SURE YOU ADD THE RIGHT ONE!
  • Take a tip on a p200 and lightly touch a colony
  • Eject the tip into the culture and put the cap on
  • For a miniprep, grow O/N for 12-16 hours NOT LONGER

[1]Openwetware

Back to Protocol