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Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer
From 2010.igem.org
Wear gloves at all times!
Requirements:
- 30% acrylamide(Biorad) NEUROTOXIN!
- 4X separation gel buffer: 1.5M Tris.HCl, 0.4% SDS pH6.8
- 4X stacking gel buffer: 0.5M Tris.HCl, 1.92M glycine, 1% SDS water saturated isobutanol
- TEMED( in yellow cabinet )
- 10% APS (Ammonium persulfate) ; prepare 10 ml and keep cold in fridge
Hoeffer Mighty gel system
