http://2010.igem.org/wiki/index.php?title=LB(_TY)_medium&feed=atom&action=historyLB( TY) medium - Revision history2024-03-29T13:25:11ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=LB(_TY)_medium&diff=56210&oldid=prevNeima: New page: '''Ingredients''' -10 g Bacto-tryptone -5 g yeast extract -10 g NaCl '''Protocol''' -Mix dry ingredients and add distilled water up to 1 Liet -Pour into 2 L flask (or greater) -Auto...2010-08-24T10:14:07Z<p>New page: '''Ingredients''' -10 g Bacto-tryptone -5 g yeast extract -10 g NaCl '''Protocol''' -Mix dry ingredients and add distilled water up to 1 Liet -Pour into 2 L flask (or greater) -Auto...</p>
<p><b>New page</b></p><div>'''Ingredients'''<br />
<br />
-10 g Bacto-tryptone<br />
<br />
-5 g yeast extract<br />
<br />
-10 g NaCl<br />
<br />
'''Protocol'''<br />
<br />
-Mix dry ingredients and add distilled water up to 1 Liet<br />
<br />
-Pour into 2 L flask (or greater)<br />
<br />
-Autoclave (liquid cycle)<br />
<br />
(250 oF,22 psi, 30 min)<br />
<br />
''Note:'' <br />
There asre two formulations of LB, Miller and Lennox, that differ in the amount of NaCl. Lenox has less salt, only 5 g/L. The Qiagen miniprep kit recommends LB with 10 g NaCl for highest plasmid yields.<br />
<br />
''Notes''<br />
We don't adjust pH of medium when we make it on the fly. However, if it is really important, pH the medium to 7.0 with 5M NaOH (~ 200 μl ).</div>Neima