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Korea U Seoul/27 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: ===Ligation=== <br> # Ligation of each parts : PyodA-mAAA, PzntA-RFP and pSB1A2 <br> {| style="border: 1px solid black;" |'''Reagent''' |'''Volume (μL)''' |- |10x T4 DNA ligase reaction ...) |
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Latest revision as of 13:34, 20 October 2010
Ligation
- Ligation of each parts : PyodA-mAAA, PzntA-RFP and pSB1A2
| Reagent | Volume (μL) |
| 10x T4 DNA ligase reaction buffer | 2 |
| T4 DNA ligase | 2 |
| Each of the digests | 2 + 2 + 2 = 8 |
| D.W. | 8 / total = 20 |
| Incubation at room temperature for 30min and stop at 80˚C for 20min | |
|---|---|
- Transformation to E. coli DH5α
