INSA-Lyon/9 September 2010
From 2010.igem.org
September 9th
DNA extraction from the liquid cultur and cultur on plate :
- All liquid culturs : 50µL
- Phasin and Curli :150µL
Digestion :
- Curli : E+S, E, negative witness (without enzyme)
- Phasin :X+P, E, negative witness
- Ligation of the 07/09 : X+P
Verification of the digestion with an agarose electrophoris gel :
- Curli and Phasin : all is OK
- Ligations : we failed the revelation of the gel because of the BET
Measure of fluorescence and DO of the liquid cultur of PHL1273.
Start of 6 new liquid culturs of PHL1273 : waterbath at 28°C, 3 tubes at 60rpm, 3 tubes at 180rpm
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