INSA-Lyon/31 August 2010
From 2010.igem.org
August 31st
Verification of the PCR :
- pha A : OK
- pha B : wrong temperature of hybridation
New PCR for pha B with a new temperature of hybridation
PCR with universel primers in order to check the ligation 1N/L2
Agarose gel of verification reveal it didn't work
Extraction of 7P and 22B
Transformation of Curli, Phasin , 7B, 24M, 22B
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