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INSA-Lyon/30 September 2010
From 2010.igem.org
September 30th
Genomic library of I0500, 10L and 4O
Agarose gel electrophoresis :
- 8C/14K :ok ->Genomic library
- 8C : nothing (!?)
- pha B : OK, purification of the band
Digestion :
- 18A, phaA : with E+S
- 14/12/13, pha B and L2/1N : with X+P
Agarose gel electrophoresis for purification : we can't see anything so we will do it again later
DNA extraction, digestion with E and agarose gel electrophoris for the 10 clones of 1F/24A/14K : the higher weights were not separated, to do again
Gel verification for plasmid backbones PCR (about 0,7%): nothing on the gel!! Maybe, PCR on the plasmid is better.
From transformation negative control, we purified XL-Blue Cells on GL, at 37°C overnight.
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