"
INSA-Lyon/30 September 2010
From 2010.igem.org
(Difference between revisions)
| Line 72: | Line 72: | ||
<p>(mise en collection) of I0500, 10L and 4O </p><br> | <p>(mise en collection) of I0500, 10L and 4O </p><br> | ||
| - | <p>Agarose gel | + | <p>Agarose gel electrophoresis : <br> |
<ol id="list_extra"> | <ol id="list_extra"> | ||
<li>8C/14K :ok (mise en collection)</li> | <li>8C/14K :ok (mise en collection)</li> | ||
| Line 83: | Line 83: | ||
<li>14/12/13, pha B and L2/1N : with X+P</li> | <li>14/12/13, pha B and L2/1N : with X+P</li> | ||
</ol><br></p> | </ol><br></p> | ||
| - | <p>Agarose gel | + | <p>Agarose gel electrophoresis for purification : we can't see anything so we will do it again later</p><br> |
| - | <p>DNA extraction, digestion with E and agarose gel electrophoris for the 10 clones of 1F/24A/14K : the higher | + | <p>DNA extraction, digestion with E and agarose gel electrophoris for the 10 clones of 1F/24A/14K : the higher weights were not separated, to do again</p><br><br> |
| + | <p>Gel verification for plasmid backbones PCR (about 0,7%): nothing on the gel!! Maybe, PCR on the plasmid is better.</p><br> | ||
| + | <p>From transformation negative control, we purified XL-Blue Cells on GL, at 37°C overnight.</p><br> | ||
| + | |||
<br><br><br><br><br><br><br> | <br><br><br><br><br><br><br> | ||
</html> | </html> | ||
Revision as of 12:30, 8 October 2010
September 30th
(mise en collection) of I0500, 10L and 4O
Agarose gel electrophoresis :
- 8C/14K :ok (mise en collection)
- 8C : nothing (!?)
- pha B : OK, purification of the band
Digestion :
- 18A, phaA : with E+S
- 14/12/13, pha B and L2/1N : with X+P
Agarose gel electrophoresis for purification : we can't see anything so we will do it again later
DNA extraction, digestion with E and agarose gel electrophoris for the 10 clones of 1F/24A/14K : the higher weights were not separated, to do again
Gel verification for plasmid backbones PCR (about 0,7%): nothing on the gel!! Maybe, PCR on the plasmid is better.
From transformation negative control, we purified XL-Blue Cells on GL, at 37°C overnight.
|
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
