INSA-Lyon/2 October 2010

From 2010.igem.org





October 2nd


DNA extraction of 14K, phasin, I0500, 4 clones of 1F/24A/14K and 1F/24A

Digestion :

  1. 14K with X+P
  2. Phasin, 1F/24A with S+P
  3. 1F/24A/14K with E+P
  4. I0500 with E

Purification on gel of 14K, phasin and 1F/24A

Agarose gel electrophoris for weight verification :

  1. I0500 : a dim band
  2. curli OK
  3. clones of 1F/24A/14K : none contain the insert

Ligation onvernight at 12°C of 1F/24A+14K









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