INSA-Lyon/2 October 2010
From 2010.igem.org
October 2nd
DNA extraction of 14K, phasin, I0500, 4 clones of 1F/24A/14K and 1F/24A
Digestion :
- 14K with X+P
- Phasin, 1F/24A with S+P
- 1F/24A/14K with E+P
- I0500 with E
Purification on gel of 14K, phasin and 1F/24A
Agarose gel electrophoris for weight verification :
- I0500 : a dim band
- curli OK
- clones of 1F/24A/14K : none contain the insert
Ligation onvernight at 12°C of 1F/24A+14K
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